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Essential oils (EO) have diverse applications, such as antibacterial and antifungal activity. However, they are susceptible to oxidation in the presence of air, light, and moisture. In addition, they are thermally unstable. In this sense, it is necessary to develop techniques to increase the lifespan of EO. In this work, EO was obtained from cloves and characterized by different techniques. The major compounds found were eugenol (83%), eugenol acetate (9%), and β-caryophyllene (8%). The EO was encapsulated by extrusion using a microfluidic device. The sodium alginate was used as wall material, and the CaCl2 solution was used as a crosslinking agent. The microcapsule presented sizes of 164.7 ± 0.3 μm, with an encapsulation yield of 64 ± 14%. Functional characteristic groups of EO were observed in the microcapsule by infrared and Raman spectroscopies. The microcapsule increased the thermal decomposition of the EO from 162 to 230 °C. Release kinetics of the capsule was performed, with an equilibrium time of 72 h and release of 54% of the EO. Finally, the pure EO and encapsulated EO-microcapsules were applied in the Aedes aegypti larvae control, showing mean lethal concentration necessary to eliminate 50% (LC50) values of 74.4 and 96.9 μg mL-1 and lethal concentration necessary to eliminate 90% (LC90) of 106.2 and 133.3 μg mL-1 for pure EO and encapsulated EO, respectively. Therefore, it was concluded that these microcapsules have the potential for application in the Aedes aegypti larvae control.

https://doi.org/10.21577/0103-5053.20230029

Morphological diversity among Brazilian Capsicum peppers
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Almeida, Breno Machado de

; Feitoza, Lidiane de Lima

; Lopes, Ângela Celis de Almeida

; Gomes, Regina Lúcia Ferreira

; Almeida, Rafael da Costa

; Martins, Lívia do Vale

; Silva, Verônica Brito da

Ciência Rural

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2023, Volume 53 N. 1 elocation e20210559

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Este trabalho teve como objetivo caracterizar e estimar a diversidade genética em 21 acessos de Capsicum pertencentes ao Banco Ativo de Germoplasma da Universidade Federal do Piauí (BAGC-UFPI), por análises uni e multivariadas. O experimento foi conduzido em telado, utilizando-se o delineamento inteiramente ao acaso, com quatro repetições, sendo uma planta por parcela. Realizou-se análise de variância (ANOVA) e comparação da média para as setes variáveis quantitativas, seguidas do agrupamentos de médias pelo teste Scott-Knott (P < 0,05). A análise dos sete descritores quantitativos e treze qualitativos foi estimada com base na distância de Gower. Posteriormente, foi realizada a análise de componentes principais e o método de agrupamento hierárquico UPGMA. Os resultados caracterizaram e identificaram uma ampla variabilidade genética intra e interespecífica relacionada ao tamanho, cor e formato dos frutos entre os genótipos brasileiros de Capsicum do banco de germoplasma de pimentas BAGC-UFPI. Os descritores utilizados foram eficientes na discriminação dos acessos de pimentas, especialmente para espécies proximamente relacionadas C. frutescens e C. chinense.

ABSTRACT: This research aimed to morphologically characterize and estimate the genetic diversity of 21 Capsicum accessions belonging to the Capsicum Germplasm Active Bank at the Universidade Federal do Piauí (BAGC-UFPI) using uni- and multivariate analysis. The experiment was carried out in a greenhouse, by completely randomized experimental design with four repetitions, with one plant per plot. Analysis of variance (ANOVA) and the comparison of means for seven quantitative variables were performed, followed by clustering the averages by the Scott-Knott test (P < 0.05). The analysis of the seven quantitative and thirteen qualitative descriptors was estimated based on the Gower distance. Later, it was performed the principal component analysis and the UPGMA hierarchical cluster method. Results characterized and identified a wide intra- and interspecific genetic variability related to the fruit size, colors, and shapes among the Brazilian Capsicum genotypes belonging to the BAGC-UFPI. The descriptors used in this research were effective in the discrimination of the pepper accessions, especially the closely related C. frutescens and C. chinense species.

https://doi.org/10.1590/0103-8478cr20210559

Oceanographic conditions of the continental slope and deep waters in Santos Basin: the SANSED cruise (winter 2019)
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Silveira, Ilson C. A. da

; Bernardo, Piero S.

; Lazaneo, Cauê Z.

; Amorim, João P. M.

; Borges-Silva, Milton

; Martins, Rafael C.

; Santos, Daniel M. C.

; Dottori, Marcelo

; Belo, Wellington C.

; Martins, Renato P.

; Guerra, Luiz A. A.

; Moreira, Daniel L.

Ocean and Coastal Research

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2023, Volume 71 Suppl. 3 elocation e23008

Abstract: En | Text: En | PDF: En

Abstract This work describes the circulation over the continental slope and the São Paulo Plateau in the Santos Basin during the SANSED winter 2019 survey. The cruise consisted of four legs in the period between June, 11 and August, 03 2019. The observed circulation is dominated by the Atlantic southwestern boundary current regime and remotely-generated anticyclones and cyclones. The former is composed by the Brazil Current, the Intermediate Western Boundary Current and their mesoscale meanders; the latter are 300km vortical rings with origin in the eastern side of the South Atlantic Basin. A Lagrangian scheme applied over satellite altimeter maps indicate that the origin of these rings is primarily the Cape Basin of South Africa. The interaction between the boundary currents, their cyclonic meanders, and the anticyclonic rings is complex, and varies widely. During the SANSED winter 2019 survey period, three anticyclones interacted with the Brazil Current, instabilizing it, forming dipoles with the current cyclonic meanders, leading to their downstream propagation. Ancienter cyclonic eddies within Santos Basin may interfere with the propagation of the large anticyclones further south. In addition, the continuous arrival of remotely-originated anticyclones, the larger portion over the São Paulo Plateau presented a tendency of counter-clockwise circulation during the whole cruise period.

https://doi.org/10.1590/2675-2824071.2206icas

Model Membranes and Antimicrobial Activities of pH-Sensitive Copolymers
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Saraiva, Greice K.

; Souza, Valdomiro V. de

; Oliveira, Luciana C. de

; Procópio, Alyne

; Martins, Maisa T.

; Aidar, Isabel N.

; Yi, Ronaldo C. F.

; Lacerda, Caroline D.

; Carretero, Gustavo P B.

; Lira, Rafael B. de

; Riske, Karin A.

; Salinas, Roberto K.

; Chaimovich, Hernan

; Florenzano, Fábio H.

; Cuccovia, Iolanda M.

Journal of the Brazilian Chemical Society

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2023, Volume 34 N. 12 Pages 1863 - 1876

Abstract: En | Text: En | PDF: En

Polymers are options as antimicrobials for skin protection, antifouling surfaces, and fabrics. Here we analyzed the interaction of polymers based on poly(methacrylate) (PMMA) and poly((dimethylamino ethyl) methacrylate) (PDMAEMA) with model membranes and bacteria. We used the homopolymers PMMA, PDMAEMA, and the diblock copolymer(s) prepared with different PMMAm:PDMAEMAn ratios (m/n). The interactions of PDMAEMA and PMMAm-b-PDMAEMAn with large unilamellar vesicles (LUVs) prepared with phosphatidylcholine and phosphatidylglycerol at different pHs, were analyzed by nuclear magnetic resonance (NMR), dynamic light scattering, and zeta potential. These polymers promoted LUVs leakage of a fluorescent probe (5,6-carboxyfluorescein) localized exclusively in the internal aqueous compartment. Interestingly, all copolymers exhibit a bell-shaped pH dependence for the polymer-induced LUVs leakage. The interaction of the positively charged polymers and the pH effect was also demonstrated using giant unilamellar vesicles. These copolymers inhibited bacterial growth in the micromolar range and can be used to prevent bacterial growth on surfaces.

https://doi.org/10.21577/0103-5053.20230079

Multidisciplinary Scientific Cruises for Environmental Characterization in the Santos Basin – Methods and Sampling Design
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Moreira, Daniel L.

; Dalto, Adriana G.

; Figueiredo JR, Alberto G.

; Valerio, Aline M.

; Detoni, Amalia M. S.

; Bonecker, Ana C. T.

; Signori, Camila N.

; Namiki, Cláudia

; Sasaki, Dalton K.

; Pupo, Daniel V.

; Silva, Danilo A.

; Kutner, Deborah S.

; Duque-Castaño, Diana C.

; Marcon, Eduardo H.

; Gallotta, Fabiana D. C.

; Paula, Fabiana S.

; Gallucci, Fabiane

; Roque, Gabriela C. F.

; Campos, Giulia S.

; Fonseca, Gustavo

; Mattos, Gustavo

; Lavrado, Helena P.

; Silveira, Ilson C. A. da

; Costa, Jessica O.

; Santos Filho, João R. dos

; Carneiro, Juliane C.

; Moreira, Julio C.F.

; Rozo, Laura

; Araujo, Leandro F.M.

; Lazzari, Letícia

; Silva, Letícia O. da

; Michelazzo, Luan S.

; Fernandes, Luciano F.

; Dottori, Marcelo

; Araújo Jr., Marcus A. G. de

; Chuqui, Mateus G.

; Ceccopieri, Milena

; Borges-Silva, Milton

; Kampel, Milton

; Bergo, Natascha M.

; Silva, Paulo V. M.

; Tura, Pedro M.

; Moura, Rafael B. de

; Romano, Renato G.

; Martins, Renato P.

; Carreira, Renato S.

; Toledo, Rodrigo G.A.

; Bonecker, Sérgio L.C.

; Disaró, Sibelle T.

; Rodrigues, Silvana V.

; Corbisier, Thais N.

; Vicente, Thaisa M.

; Paiva, Vitor G. de

; Pellizari, Vivian H.

; Belo, Wellington C.

; Brandini, Frederico P.

; Sousa, Silvia H.M

Ocean and Coastal Research

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2023, Volume 71 Suppl. 3 elocation e23022

Abstract: En | Text: En | PDF: En

Abstract The Santos Basin (SB) is the main petroliferous basin in the Brazilian continental margin and one of the most studied marine areas in Brazil. However, historical data suggest that new efforts should be carried out to acquire quantitative biological data, especially in the deep sea, to establish the baseline of essential ocean variables in different ecosystems for future monitoring programs. The Brazilian energy company Petrobras planned and executed 24 oceanographic cruises over a period of 2 years to assess the benthic (SANSED cruise) and pelagic (SANAGU cruise) systems of the SB (356 days at sea in 2019 and 2021/2022). These efforts were part of the Santos Project, which comprised a comprehensive environmental study aimed at investigating benthic and pelagic variables to characterize ecology, biogeochemistry, thermohaline properties of water masses, and ocean circulation patterns, geomorphology, and sedimentology, as well as organic and inorganic chemistry. Here we present the detailed sampling designs and the field methods employed on board, during the SB scientific cruises. All sampling protocols were based on standardized approaches. For the benthos analyses, triplicate sediment samples were performed using a GOMEX-type box corer (0.25 m²) or a large modified Van Veen grab (0.75 m²) at 100 stations ranging from 25 to 2400 m depth. At each station, 25 geochemical and physico-chemical parameters were analyzed in addition to micro-, meio-, and macrofauna and living foraminifera samples. For the pelagic system, 60 stations were selected to investigate the plankton community, ranging in size from pico- to macroplankton, through vertical, horizontal, and oblique net hauls (20, 200, and 500 μm mesh size), as well as 25 biogeochemical parameters collected with an aid of a CTD-rosette sampler. Part of this scientific information also serves the Regional Environmental Characterization Project (PCR-BS) in support of Petrobras’ Santos Basin drilling licensing process led by the Brazilian Environmental Agency – IBAMA. This project contributes to the sustainable development of the SB, in line with the guidelines of the United Nations Decade of Ocean Science for Sustainable Development.

https://doi.org/10.1590/2675-2824071.22072dlm

COMBINING DIFFERENT CONCURRENT TRAINING METHODS IN OLDER ADULTS WITH METABOLIC SYNDROME
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Da Silva, Marco Antônio R.

; França, Elias de

; Baptista, Liliana C.

; Neves, Rafael S.

; Loureiro, Helena

; Caperuto, Erico C.

; Veríssimo, Manuel T.

; Martins, Raul A.

Revista Brasileira de Medicina do Esporte

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aug 2022, Volume 28 N. 4 Pages 267 - 273

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introduction: Introdução: Não há estudos que comparem diferentes rotinas de treinamentos concorrentes [isto é, programa de treinamento resistido mais treinamento intervalado de alta intensidade (TR+HIIT) ao TR mais treinamento contínuo de intensidade moderada (TR+MICT)] na aptidão cardiorrespiratória (ACR) e desempenho físico em idosos com síndrome metabólica (SM). Objetivos: O objetivo deste estudo foi comparar os efeitos de duas rotinas de treinamentos concorrentes (TR+HIIT vs. TR+MICT) na ACR e aptidão física em adultos e idosos com SM. Métodos: Trinta e nove participantes (67,0 ± 6,7 anos) foram divididos randomicamente em três grupos: TR+MICT, TR+HIIT e grupo controle (CON, sem exercício). O programa de treinamento físico de 12 semanas consistiu em 3 sessões/semana (~ 50 minutos/sessão). O componente de treinamento aeróbico (HIIT ou MICT) foi adaptado para gasto energético isocalórico (P ≥ 0,217) e distribuição de isocarga (TRIMP; P ≥ 0,893), porém, o componente MICT teve tempo de treinamento aeróbico maior (P ≤ 0,01). Avaliamos a ACR pré e pós-intervenção (VO2pico), capacidade física (potência de membros inferiores [PMI], velocidade de corrida e tempo de corrida até a exaustão), lactato plasmático, percepção subjetiva de esforço (PSE), frequência cardíaca de repouso (FCrepouso) e máxima (FCpico). Resultados: No final da intervenção, somente o grupo TR+HIIT teve aumento significativo do VO2pico (P = 0,01), mas em comparação com o grupo CON, ambos os grupos TR+HIIT e TR+MICT tiveram melhora significativa do VO2pico (P ≤ 0,02). Somente o grupo TR+HIIT mostrou FCrepouso menor em comparação com o basal e com o grupo CON. Além disso, os grupos TR+HIIT e TR+MICT, mas não o grupo CON, tiveram melhora significativa da velocidade de corrida, do tempo até a exaustão e da PMI (P < 0,05). Não houve alterações significativas em FCpico, PSE e lactato em nenhum dos grupos. Conclusão: Nossos resultados sugerem que 12 semanas de TR+MICT ou TR+HIIT melhoram o desempenho físico dos membros inferiores. Além disso, nossos resultados sugerem que TR+HIIT fornece benefícios adicionais de ACR em idosos com SM. Nível de evidência II; Estudo Experimental Randomizado.

ABSTRACT Introduction: There are no studies comparing the effects of different concurrent training routines [i.e., a resistance training plus high-intensity interval training (RT+HIIT) program compared to an RT plus moderate-intensity continuous training (RT+MICT) program] on cardiorespiratory fitness (CRF) and physical performance in older adults with metabolic syndrome (MetS). Objective: This study aimed to compare the effects of two concurrent training routines, RT+HIIT versus RT+MICT, on CRF and physical fitness in adults and older adults with MetS. Methods: Thirty-nine participants (67.0±6.7 years of age) were randomly assigned into three groups: RT+ MICT, RT+ HIIT and the control group (CON, no exercise). The 12-week physical training program consisted of 3 sessions/week (~50 minutes/session). The aerobic training component was customized for iso-caloric energy expenditure (P≥0.217) and iso-load distribution (TRIMP, P≥0.893), however the MICT component had a longer aerobic training time (P≤0.01). We evaluated pre- and post-evaluation CRF (VO2peak), physical capacity [power of lower limbs (PLL), running velocity, and running time to exhaustion], plasma lactate, rate of perceived exertion (RPE), resting heart rate (HRrest), and peak heart rate (HRpeak). Results: At the end of the intervention, a significant increase in the VO2peak was observed only in the RT+HIIT group (P=0.01), but compared to the CON group both RT+HIIT and RT+MICT groups had significant improvement in the VO2peak (P≤0.02). Only the RT+HIIT group had lower HRrest as compared to the baseline and the CON group. Also, both RT+HIIT and RT+MICT groups, but not CON, significantly improved running speed, time to exhaustion, and PLL (P<0.05). There were no significant changes in HRpeak, RPE, and lactate for any of the groups. Conclusion: Our results suggest that 12 weeks of RT+MICT or RT+HIIT improve lower-limb physical performance. Also, our results suggest that RT+HIIT provides additional CRF benefits in older adults with MetS. Level of evidence II; Randomized Experimental Study.

RESUMEN Introducción: No hay estudios que comparen diferentes rutinas de entrenamientos concurrentes [es decir, programa del entrenamiento de resistencia (ER) más entrenamiento de intervalos de alta intensidad (ER+HIIT) con el ER más entrenamiento continuo de intensidad moderada (ER+ECIM) en la aptitud cardiorrespiratoria (ACR) y desempeño físico en ancianos con síndrome metabólico (SM). Objetivo: El objetivo de este estudio fue comparar los efectos de dos rutinas de entrenamientos concurrentes (ER+HIIT sobre ER+ECIM) sobre la ACR y aptitud física en ancianos con SM. Método: Treinta y nueve participantes (67,0±6,7 años) fueron divididos aleatoriamente en 3 grupos: ER+ECIM, ER+HIIT y grupo de control (CON, sin ejercicio). El programa de entrenamiento físico de 12 semanas consistió en 3 sesiones semanales (50 minutos/sesión). En componente de entrenamiento aeróbico HIIT o ECIM se igualó en cuanto al gasto energético isocalórico (P≥0,217) y la distribución de isocarga (TRIMP; P≥0,893), sin embargo, el componente ECIM tuvo un mayor tiempo de entrenamiento aeróbico (P≤0,01). Se evaluó la ACR antes y después de la intervención (VO2pico), la capacidad física (potencia de las extremidades inferiores [PEI], la velocidad, y el tiempo hasta el agotamiento), el lactato plasmático y percepción subjetiva del esfuerzo (RPE), la frecuencia cardíaca en reposo HRest) y máxima (FCpico). Resultados: Al final de la intervención, solo el grupo ER+HIIT presentó un aumento significativo del VO2pico (P=0,01), pero en comparación con el grupo CON, tanto el grupo ER+HIIT como el ER+ECIM presentaron una mejora significativa l del VO2pico (P ≤ 0,02).. Sólo el grupo ER+HIIT demostró una HRest menor en comparación con el nivel de referencia y el grupo CON. Además, los grupos ER+HIIT y ER+ECIM, pero no el grupo CON, tuvieron una mejora significativa en la velocidad de carrera, el tiempo hasta el agotamiento y la PEI (P<0,05). No hubo cambios significativos en FCpico, RPE y lactato para ninguno de los grupos. Conclusión: Nuestros resultados sugieren que 12 semanas de ER+ECIM o ER+HIIT mejoran el rendimiento físico de las extremidades inferiores. Además, sugieren que ER+HIIT proporciona beneficios adicionales en la ACR en ancianos con SM. Nivel de evidencia II;Estudio experimental aleatorio

https://doi.org/10.1590/1517-8692202228042020_0122

Brazilian practice guidelines for stroke rehabilitation: part II
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Minelli, Cesar

; Luvizutto, Gustavo José

; Cacho, Roberta de Oliveira

; Neves, Luciana de Oliveira

; Magalhães, Sheila Cristina Sayuri Abe

; Pedatella, Marco Túlio Araújo

; Mendonça, Lucia Iracema Zanotto de

; Ortiz, Karin Zazo

; Lange, Marcos Christiano

; Ribeiro, Priscila Watson

; Souza, Luciane Aparecida Pascucci Sande de

; Milani, Cristiano

; Cruz, Daniel Marinho Cezar da

; Costa, Rafael Dalle Molle da

; Conforto, Adriana Bastos

; Carvalho, Fernanda Martins Maia

; Ciarlini, Bruna Silva

; Frota, Norberto Anizio Ferreira

; Almeida, Kelson James

; Schochat, Eliane

; Oliveira, Tatiana de Paula

; Miranda, Camila

; Piemonte, Maria Elisa Pimentel

; Lopes, Laura Cardia Gomes

; Lopes, Camila Galvão

; Tosin, Michelle Hyczy de Siqueira

; Oliveira, Bianca Campos

; Oliveira, Beatriz Guitton Renaud Baptista de

; Castro, Shamyr Sulyvan de

; Andrade, João Brainier Clares de

; Silva, Gisele Sampaio

; Pontes-Neto, Octávio Marques

; Carvalho, João José Freitas de

; Martins, Sheila C. Ouriques

; Bazan, Rodrigo

Arquivos de Neuro-Psiquiatria

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jul 2022, Volume 80 N. 7 Pages 741 - 758

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract The Brazilian Practice Guidelines for Stroke Rehabilitation – Part II, developed by the Scientific Department of Neurological Rehabilitation of the Brazilian Academy of Neurology (Academia Brasileira de Neurologia, in Portuguese), focuses on specific rehabilitation techniques to aid recovery from impairment and disability after stroke. As in Part I, Part II is also based on recently available evidence from randomized controlled trials, systematic reviews, meta-analyses, and other guidelines. Part II covers disorders of communication, dysphagia, postural control and balance, ataxias, spasticity, upper limb rehabilitation, gait, cognition, unilateral spatial neglect, sensory impairments, home rehabilitation, medication adherence, palliative care, cerebrovascular events related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the future of stroke rehabilitation, and stroke websites to support patients and caregivers. Our goal is to provide health professionals with more recent knowledge and recommendations for better rehabilitation care after stroke.

Resumo As Diretrizes Brasileiras de Reabilitação do Acidente Vascular Cerebral (AVC) - Parte II, desenvolvida pelo Departamento Científico de Reabilitação Neurológica da Academia Brasileira de Neurologia é voltada para intervenções específicas de técnicas de reabilitação de déficits neurológicos e incapacidades. Seguindo o mesmo modelo da Parte I, a Parte II também se baseia em estudos randomizados, revisões sistemáticas, metanálises e outras diretrizes sobre o mesmo tema. A segunda parte aborda os distúrbios da comunicação, disfagia, controle postural e equilíbrio, ataxias, espasticidade, reabilitação do membro superior, marcha, cognição, negligência espacial unilateral, déficits sensoriais, reabilitação domiciliar, aderênciaao usode medicamentos, cuidados paliativos,ofuturodareabilitação no AVC, e websites de orientação sobre AVC para pacientes e cuidadores. Nosso objetivo é fornecer aos profissionais envolvidos na reabilitação conhecimento atualizado e recomendações para um melhor cuidado no pós-AVC.

https://doi.org/10.1055/s-0042-1757692

Selection of F2RC1 saladette-type dwarf tomato plant populations for fruit quality and whitefly resistance
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Oliveira, Camila S. de

; Maciel, Gabriel M.

; Siquieroli, Ana C. S.

; Gomes, Danilo A.

; Martins, Marcos P. do C.

; Finzi, Rafael R.

Revista Brasileira de Engenharia Agrícola e Ambiental

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jan 2022, Volume 26 N. 1 Pages 28 - 35

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O objetivo do estudo foi selecionar populações F2RC1 de tomateiro anão do tipo saladete promissoras para o desenvolvimento de linhagens baseado em características agronômicas, qualidade de fruto e resistência a mosca branca. O delineamento experimental foi em blocos casualizados contendo 13 tratamentos (10 populações F2RC1 de tomateiro anão, genitores e híbrido comercial) com quatro repetições. Foram avaliados: peso, comprimento, diâmetro, formato, espessura da polpa, número de lóculos, teores de sólidos solúveis, β-caroteno e licopeno do fruto; comprimento do entrenó da planta; teores de acilaçúcares, número de ovos, ninfas e adultos de mosca branca nos folíolos. Os dados foram analisados por ANOVA, índices de seleção e análise multivariada. O primeiro retrocruzamento aumentou as características agronômicas das populações em relação ao genitor doador, principalmente para peso de fruto (169.1%), comprimento do fruto (26.1%) e diâmetro do fruto (16.6%). As populações UFU SDi 7, UFU SDi 9 e UFU-SDi 17 foram selecionadas por dois índices de seleção e são consideradas promissoras.

ABSTRACT This study aimed to select promising F2RC1 populations of saladette-type dwarf tomato plants for the development of breeding lines based on agronomic characteristics, fruit quality, and whitefly resistance. The experimental design was randomized blocks containing 13 treatments (10 F2RC1 populations of dwarf tomato plants, both parents, and a commercial hybrid) with four replicates. The evaluations were performed included weight, length, diameter, shape, pulp thickness, number of locules, soluble solids, β-carotene, and lycopene concentration of the fruit; plant internode length; acylsugars concentration; and number of whitefly eggs, nymphs, and adults on the leaflets. The data were analyzed using ANOVA, selection indices, and multivariate analysis. The first backcross increased the agronomic characteristics of the populations in relation to the donor parent, especially for fruit weight (169.1%), fruit length (26.1%), and fruit diameter (16.6%). The UFU SDi 7, UFU SDi 9, and UFU-SDi 17 populations were selected using two selection indices and were therefore considered promising.

https://doi.org/10.1590/1807-1929/agriambi.v26n1p28-35 42 downloads

Harvesting of Chlorella sorokiniana BR001 cultivated in a low-nitrogen medium using different techniques
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Lira, Rafael de Araújo

; Corrêdo, Lucas de Paula

; Soares, Jimmy

; Rocha, Mariana Machado

; Matos, Antonio Teixeira de

; Coimbra, Jane Selia dos Reis

; Martins, Marcio Arêdes

Ciência Rural

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2022, Volume 52 N. 1 elocation e20200293

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O processo de colheita é um desafio atual para a produção comercial de microalgas porque a biomassa é diluída no meio de cultivo. Diversos métodos têm sido propostos para coletar células de microalgas, porém não existe um consenso sobre um método ótimo para tal aplicação. Neste estudo, métodos baseados em sedimentação, floculação e centrifugação foram avaliados na recuperação de Chlorella sorokiniana BR001 cultivada em um meio com baixo teor de nitrogênio. C. sorokiniana BR001 foi cultivada em um meio com baixo teor de nitrogênio para induzir ao acúmulo de lipídeos e carboidratos neutros. A biomassa de C. sorokiniana BR001 cultivada em um meio com baixo teor de nitrogênio apresentou um teor de lipídeos 1,9 vezes superior (23,8 ± 4,5%), quando comparada à biomassa produzida em um meio com alto teor de nitrogênio (12,3 ± 1,2%). Adicionalmente, a biomassa da linhagem BR001 cultivada em um meio com baixo teor de nitrogênio apresentou alto teor de carboidratos neutros (52,1 ± 1,5%). O processo baseado em sedimentação natural foi avaliado utilizando uma coluna de sedimentação e concluiu-se que C. sorokiniana BR001 é uma linhagem não floculante. Portanto, o efeito de diferentes concentrações de sulfato férrico (0,005 a 1 g L-1) ou sulfato de alumínio (0,025 a 0,83 g L-1) foram avaliados no processo de floculação de C. sorokiniana BR001, mas altas doses de floculantes foram necessárias para uma colheita de biomassa eficiente. Também foi avaliada a centrifugação em baixa velocidade (300 a 3.000 g), e foi possível concluir que este processo constituiu o mais adequado para a colheita da linhagem não floculante C. sorokiniana BR001.

ABSTRACT: The harvesting process is a current challenge for the commercial production of microalgae because the biomass is diluted in the culture medium. Several methods have been proposed to harvest microalgae cells, but there is not a consensus about the optimum method for such application. Herein, the methods based on sedimentation, flocculation, and centrifugation were evaluated on the recovery of Chlorella sorokiniana BR001 cultivated in a low-nitrogen medium. C. sorokiniana BR001 was cultivated using a low-nitrogen medium to trigger the accumulation of neutral lipids and neutral carbohydrates. The biomass of C. sorokiniana BR001 cultivated in a low-nitrogen medium showed a total lipid content of 1.9 times higher (23.8 ± 4.5%) when compared to the biomass produced in a high-nitrogen medium (12.3 ± 1.2%). In addition, the biomass of the BR001 strain cultivated in a low-nitrogen medium showed a high content of neutral carbohydrates (52.1 ± 1.5%). The natural sedimentation-based process was evaluated using a sedimentation column, and it was concluded that C. sorokiniana BR001 is a non-flocculent strain. Therefore, it was evaluated the effect of different concentrations of ferric sulfate (0.005 to 1 g L-1) or aluminum sulfate (0.025 to 0.83 g L-1) on the flocculation process of C. sorokiniana BR001, but high doses of flocculant agents were required for an efficient harvest of biomass. It was evaluated the centrifugation at low speed (300 to 3,000 g) as well, and it was possible to conclude that this process was the most adequate to harvest the non-flocculent strain C. sorokiniana BR001.

https://doi.org/10.1590/0103-8478cr20200293 300 downloads

10.

Preparation of Fluorescent Carbon-Based Dots from Waste Tire Pyrolysis
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Parra, Juliana B.

; Silva, Karina C.

; Valezin, Pedro A. S.

; Martins, Raphael G.

; Gomes, Rodolfo R.

; Pereira, Rafael S.

; Melo, Fernando M. de

; Morandim-Giannetti, Andreia de A.

; Santos, Ronaldo G. dos

; Panzarini, Luz Consuelo G. A.

; Toyama, Marcos M.

Journal of the Brazilian Chemical Society

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nov 2020, Volume 31 N. 11 Pages 2224 - 2231

Abstract: En | Text: En | PDF: En

The pyrolysis of waste tire has been proposed as an alternative route to its recycling producing residual oils that can be used as fuels. In this work, a physicochemical process to obtain carbon-based dots (CBD) from carbon black (CBL) derivatives of tires is described. For this, the CBL was treated with different acids to produce CBL1 and CBL2 particles suspension. The pH of the suspension was adjusted to 5.6 to precipitate remaining impurities. Afterwards, the supernatant was ultra-centrifuged and the yellow pale solid, the purified CBD obtained by roto evaporation, was characterized by ultraviolet-visible spectrophotometer (UV-Vis), photoluminescence (PL) at 320-380 nm, high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared microscopy (mFTIR), and X-ray photoelectron spectroscopy (XPS). This material showed the typical excitation wavelength dependent emission and a PL quantum yield of 19.29% and was constituted by a well-defined spherical shaped and single sheets of “graphene quantum dots” with undefined shape, with potential applications in optoelectronic and fluorescence imaging devices.

https://doi.org/10.21577/0103-5053.20200072 428 downloads

11.

Torres, Marie-Hélène C. (Org.). Estudos da tradução intercontinentais: Brasil – Canadá – Romênia. Florianópolis: Rafael Copetti, 2019, 306 p.
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Martins, Lia

Cadernos de Tradução

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aug 2020, Volume 40 N. 2 Pages 322 - 326

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5007/2175-7968.2020v40n2p322 140 downloads

12.

Tenofovir Disoproxil Fumarate: New Chemical Developments and Encouraging in vitro Biological Results for SARS-CoV-2
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Clososki, Giuliano C.

; Soldi, Rafael A.

; Silva, Rodrigo M. da

; Guaratini, Thais

; Lopes, José N. C.

; Pereira, Pâmela R. R.

; Lopes, João L. C.

; Santos, Thiago dos

; Martins, Ronaldo B.

; Costa, Cristina S.

; Carvalho, Andréia N. de

; da Silva, Luis L. P.

; Arruda, Eurico

; Lopes, Norberto P.

Journal of the Brazilian Chemical Society

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aug 2020, Volume 31 N. 8 Pages 1552 - 1556

Abstract: En | Text: En | PDF: En

The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led society to live with a serious public health problem. In this sense, repositioning of antiretrovirals has captured the attention of the scientific community. Tenofovir disoproxil fumarate (TDF) is an antiretroviral compound that is used to treat acquired immune deficiency syndrome (AIDS) and hepatitis B. In this short report, we present a scale-up investigation of TDF by in situ infrared spectroscopy monitoring and a forced degradation study to describe a new degradation product. Finally, we have evaluated TDF in vitro for SARS-CoV-2 for the first time foreseeing the using of this medicine in pre-clinical and clinical investigations for the COVID-19 (coronavirus disease 2019) treatment.

https://doi.org/10.21577/0103-5053.20200106 1823 downloads

13.

Seroprevalence of Corynebacterium pseudotuberculosis and Toxoplasma gondii in sheep in semi-arid region of Ceará state
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Moura, Gabriela Hémylin Ferreira

; Lelis, Ivana Cristina Nunes Gadelha

; Rocha, Célio Souza da

; Oliveira, Ilanna Vanessa Pristo de Medeiros

; Bezerra, José Artur Brilhante

; Calabuig, Cecilia Irene Pérez

; Martins, Patrícia Yoshida Faccioli

; Pinheiro, Raymundo Rizaldo

; Sousa, Maxmiana Mesquita de

; Santos, Vanderlan Warlington Souza dos

; Abreu, Antônio Rafael Albuquerque

; Antunes, João Marcelo Azevedo de Paula

Ciência Rural

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2020, Volume 50 N. 9 elocation e20190760

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: O presente estudo foi conduzido para investigar a soroprevalência em 20 fazendas de criação extensiva de ovinos quanto à presença de anticorpos para Corynebacterium pseudotuberculosis (n=402) e Toxoplasma gondii (n=228). Ensaio de imunoabsorção enzimático (ELISA) foi utilizado para a detecção de anticorpos contra C. pseudotuberculosis/T. gondii. Observou-se que C. pseudotuberculosis apresentou a maior prevalência na região (34,07%), com presença estatisticamente significante (p <0,05) nas categorias de ovelha. Anticorpos contra T. gondii foram encontrados em 14,91% dos animais estudados. Sobre a coinfecção de C. pseudotuberculosis/T. gondii, as categorias carneiro apresentaram diferenças significativas (p <0,05), sugerindo que esse gênero poderia perpetuar as doenças nos rebanhos. Concluiu-se que o conhecimento sobre as doenças na região em estudo facilitaria a execução de medidas profiláticas, principalmente contra as doenças que apresentam riscos à saúde pública e causam danos ao produtor.

ABSTRACT: The present study was conducted to investigate in 20 extensive sheep farms for the seroprevalence of Corynebacterium pseudotuberculosis (n=402) and Toxoplasma gondii (n=228). Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to C. pseudotuberculosis/T. gondii. It was observed that C. pseudotuberculosis showed the highest prevalence in the region (34.07%) with statistically significant presence (p<0.05) in ewes. Antibodies to T. gondii was reported in 14.91% of the animals studied. About C. pseudotuberculosis/T. gondii coinfection the categories of rams showed significant (p<0.05) differences, suggesting that this gender could perpetuate the diseases in the flocks. It was concluded that the knowledge about the diseases in the region under study would facilitate the execution of prophylactic measures, especially against the diseases that pose risks to the public health and cause damages to the producer.

https://doi.org/10.1590/0103-8478cr20190760 722 downloads

14.

Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis
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Fonseca, Priscilla Duarte Marques

; Corral, Marcelo Andreeta

; Meisel, Dirce Mary C. Lima

; Levi, Debora

; Nascimento, Rafael Correa

; Castro-Borges, William

; Gryschek, Ronaldo

; Costa-Cruz, Julia Maria

; Paula, Fabiana Martins de

Revista do Instituto de Medicina Tropical de São Paulo

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2020, Volume 62 elocation e23

Abstract: En | Text: En | PDF: En

ABSTRACT In experimental infection with Strongyloides venezuelensis, the acute and recovery phases can be distinguished, unlike human infections caused by Strongyloides stercoralis. The objective of this study was to evaluate the production of anti-Strongyloides IgG antibodies and the recognition of immunogenic protein bands during the acute and the recovery phases in rats experimentally infected with S. venezuelensis. Rats were infected subcutaneously with 400 or 4,000 S. venezuelensis infective larvae. The acute phase was characterized by elimination of a large number of eggs in the faeces on days 6-14 post infection; the recovery phase was characterized by the resolution of the infection between days 30 and 35 post infection. Differences in IgG levels were observed in the acute and the recovery phases. Different antigenic fractions were recognized in both phases of infection. It is concluded that proteins within the 30-40 kDa range are immunoreactive markers for both the acute and the recovery phases in rats experimentally infected with S. venezuelensis, particularly using membrane antigen.

https://doi.org/10.1590/s1678-9946202062023 373 downloads

15.

Type 2 diabetes mellitus alters cardiac mitochondrial content and function in a non-obese mice model
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YURRE, AINHOA R. DE

; MARTINS, EDUARDA G.L.

; LOPEZ-ALARCON, MICAELA

; CABRAL, BRUNO

; VERA, NARENDRA

; LOPES, JARLENE A.

; GALINA, ANTONIO

; TAKIYA, CHRISTINA M.

; LINDOSO, RAFAEL S.

; VIEYRA, ADALBERTO

; SÁENZ, OSCAR C.

; MEDEI, EMILIANO

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 2 elocation e20191340

Abstract: En | Text: En | PDF: En

Abstract Type 2 diabetes mellitus (T2DM) is associated with an increase of premature appearance of several disorders such as cardiac complications. Thus, we test the hypothesis that a combination of a high fat diet (HFD) and low doses of streptozotocin (STZ) recapitulate a suitable mice model of T2DM to study the cardiac mitochondrial disturbances induced by this disease. Animals were divided in 2 groups: the T2DM group was given a HFD and injected with 2 low doses of STZ, while the CNTRL group was given a standard chow and a buffer solution. The combination of HFD and STZ recapitulate the T2DM metabolic profile showing higher blood glucose levels in T2DM mice when compared to CNTRL, and also, insulin resistance. The kidney structure/function was preserved. Regarding cardiac mitochondrial function, in all phosphorylative states, the cardiac mitochondria from T2DM mice presented reduced oxygen fluxes when compared to CNTRL mice. Also, mitochondria from T2DM mice showed decreased citrate synthase activity and lower protein content of mitochondrial complexes. Our results show that in this non-obese T2DM model, which recapitulates the classical metabolic alterations, mitochondrial function is impaired and provides a useful model to deepen study the mechanisms underlying these alterations.

https://doi.org/10.1590/0001-3765202020191340 862 downloads

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