Jabuticaba-Induced Endothelium-Independent Vasodilating Effect on Isolated Arteries

Abstract

Background:

Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified.

Objectives:

To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries.

Methods:

Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K⁺ channels and Ca²⁺ intra- and extracellular sources on JHE-stimulated response were assessed.

Results:

Arteries pre-contracted with phenylephrine showed concentration-dependent relaxation (0.380 to 1.92 mg/mL). Treatment with K⁺ channel blockers (tetraethyl-ammonium, glibenclamide, 4-aminopyridine) hindered relaxation due to JHE. In addition, phenylephrine-stimulated contraction was hindered by previous treatment with JHE. Inhibition of sarcoplasmic reticulum Ca²⁺ ATPase did not change relaxation due to JHE. In addition, JHE inhibited the contraction caused by Ca²⁺ influx stimulated by phenylephrine and KCl (75 mM).

Conclusion:

JHE induces endothelium-independent vasodilation. Activation of K⁺ channels and inhibition of Ca²⁺ influx through the membrane are involved in the JHE relaxant effect.

Keywords:
Jabuticaba (Myrciaria Cauliflora); Trees; Vasodilatation; Calcium Channels; Muscle, Smooth Vascular