[Standardization of the Sperm Class Analyzer<sup>®</sup> (SCA<sup>®</sup>) for the evaluation of cryopreserved equine sperm]

Snoeck, P.P.N.; Pessoa, T.H.O.; Barros, C.H.S.C.; Allaman, I.B.

doi:10.1590/1678-4162-11665

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Medicina Veterinária • Arq. Bras. Med. Vet. Zootec. 72 (04) • Jul-Aug 2020 • https://doi.org/10.1590/1678-4162-11665 copy

[Standardization of the Sperm Class Analyzer^® (SCA^®) for the evaluation of cryopreserved equine sperm]

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The objective of the first experiment was to evaluate the effect of 25, 30 and 50Hz frame acquisition rate on equine cryopreserved sperm. All frame acquisition rates tested were adequate to capture the sperm movement (P>0.05). The aim of the second experiment was to evaluate the effect of chambers, slide-coverslip, Leja^®10 and 20 on sperm movement. The use of slide-coverslip was superior to maintain LIN and WOB (P<0.05). The aim of the third experiment was to evaluate the effect of 25, 50 and 100x10⁶ sperm/mL concentration on sperm movement. Concentrations of 25 and 50x10⁶ sperm/mL were greater than 100x10⁶ to preserve LIN, STR and BCF and did not adversely affect motility (P<0.05). The aim of the fourth experiment was to evaluate the effect of BotuCrio^®, BotuSêmen^®, TALP sperm and physiological solution on sperm movement. BotuCrio^® was superior among other extenders in preserving BCF and hyperactive (P<0.05). It is concluded that the use of the frame acquisition rate between 25 and 50 Hz; the deposition of semen between slide and coverslip and new dilution in the freezing extender to 25-50x10⁶ of sperm/mL is ideal to reliably evaluate cryopreserved equine semen by SCA^®.

Keywords:
freezing; semen; stallion; computer-assisted sperm analysis (CASA)

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[1] E-mail: paolasnoeck@uesc.br

Parâmetros	Velocidade de captura de 25 imagens			Erro Padrão
	25Hz	30Hz	50Hz
MT (%)	45,0	44,2	42,2	3,06
MP (%)	21,4	21,8	19,4	2,13
VCL (µm/s)	51,4	53,1	50,5	1,93
VSL (µm/s)	27,6	28,3	27,4	1,41
VAP (µm/s)	32,7	33,0	32,4	1,49
LIN (%)	53,3	52,4	54,0	1,97
STR (%)	83,4	83,2	83,7	1,03
WOB (%)	63,3	62,9	63,9	1,65
ALH (µm)	2,4	2,4	2,3	0,04
BCF (Hz)	15,9	15,6	15,8	0,71
HIPERATIVO (%)	6,8	7,3	5,9	0,91

Parâmetros	Lâmina/lamínula	Leja^® 10µm	Leja^® 20µm	Erro Padrão
MT (%)	32,0	28,9	29,8	2,94
MP (%)	11,7^a	9,9^a	8,3^b	1,55
VCL (µm/s)	42,8^b	46,7^a	39,2^c	2,08
VSL (µm/s)	19,8^a	19,8^a	16,2^b	1,34
VAP (µm/s)	24,7^a	25,7^a	20,8^b	1,34
LIN (%)	45,4^a	41,4^b	40,5^b	1,68
STR (%)	78,4^a	74,9^b	76,2^ab	1,65
WOB (%)	57,7^a	54,5^b	52,7^b	1,17
ALH (µm)	2,2^b	2,3^a	2,2^ab	0,07
BCF (Hz)	16,8	16,7	16,6	0,77
HIPERATIVO (%)	3,1	3,0	2,2	0,61

Parâmetros	Concentração espermática			Erro Padrão
	25 x10⁶/mL	50 x10⁶/mL	100 x10⁶/mL
MT (%)	49,2^b	52,1^b	65,6^a	3,17
MP (%)	17,7^b	18,1^b	22,4^a	2,28
VCL (µm/s)	51,0	50,4	52,9	2,92
VSL (µm/s)	19,5	18,9	18,8	1,42
VAP (µm/s)	25,9	25,6	26,9	1,66
LIN (%)	36,7^a	35,6^a	33,9^b	0,95
STR (%)	72,7^a	71,0^a	67,5^b	1,33
WOB (%)	50,1	49,8	49,9	0,55
ALH (µm)	2,3	2,2	2,2	0,08
BCF (Hz)	20,5^a	20,3^a	19,1^b	0,71
HIPERATIVO (%)	4,5	4,7	5,0	0,8

	Diluidor				Erro Padrão
Parâmetros	BotuCrio^®	BotuSêmen^®	TALP	SolFi
MT (%)	22,9^a	18,7^b	20,8^ab	19,9^ab	1,82
MP (%)	7,8^a	5,3^b	5,7^ab	4,7^b	0,95
VCL (µm/s)	37,1^a	32,6^bc	35,6^ab	31,2^c	1,83
VSL (µm/s)	20,4^a	17,6^ab	19,5^a	15,1^b	1,39
VAP (µm/s)	24,5^ab	21,4^bc	25,4^a	19,1^c	1,5
LIN (%)	53,2^a	50,3^ab	53,3^a	46,7^b	1,65
STR (%)	81,5^a	78,5^ab	75,8^b	77,1^b	1,22
WOB (%)	64,7^b	63,1^bc	69,6^a	60,0^c	1,29
ALH (µm)	2,4^a	2,1^ab	2,0^b	2,2^ab	0,12
BCF (Hz)	10,5^a	9,1^b	7,9^b	8,3^b	0,46
HIPERATIVO (%)	3,5^a	2,2^b	1,8^b	2,2^b	0,46

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[Standardization of the Sperm Class Analyzer® (SCA®) for the evaluation of cryopreserved equine sperm]

ABSTRACT

[Standardization of the Sperm Class Analyzer^® (SCA^®) for the evaluation of cryopreserved equine sperm]