[Linfoma extranodal em um cachorro-do-mato-vinagre (Speothos venaticus) - relato de caso]

Um cachorro-do-mato-vinagre (Speothos venaticus), de sete anos de idade, mantido em cativeiro, foi diagnosticado com linfoma que havia afetado os rins, as adrenais, o fígado e o baço. O animal desenvolveu insuficiência renal e foi submetido à eutanásia devido ao prognóstico desfavorável. Macroscopicamente, ambos os rins estavam aumentados de tamanho, com múltiplos nódulos. Histologicamente, a neoplasia era infiltrativa, pobremente delimitada e constituída por células redondas. Duas populações distintas foram observadas: células pequenas com morfologia linfocítica e células grandes e pleomórficas. A maior parte da população de células pequenas, correspondendo a aproximadamente 40% da população celular na neoplasia, foi positiva para CD3. As células neoplásicas foram positivas para CD45, CD11d e granzima B e negativas para CD20, CD79a, PAX5, CD163 e mieloperoxidase. Este é o primeiro caso de linfoma em um cachorro-do-mato-vinagre. Tal relado demonstra a utilidade de vários marcadores de superfície celular para o diagnóstico diferencial de tumores de células redondas nessa espécie.

Palavras-chave:
linfoma; imuno-histoquímica; neoplasma; animal silvestre

Autoria

T.F. Carvalho

Escola de Veterinária ˗ Universidade Federal de Minas Gerais ˗ Belo Horizonte, MGUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilEscola de Veterinária ˗ Universidade Federal de Minas Gerais ˗ Belo Horizonte, MG

https://orcid.org/0000-0002-3053-7242

Â.T. Pessanha

Fundação de Parques Municipais e Zoobotânica de Belo Horizonte ˗ Belo Horizonte, MGFundação de Parques Municipais e Zoobotânica de Belo HorizonteBrazilBelo Horizonte, MG, BrazilFundação de Parques Municipais e Zoobotânica de Belo Horizonte ˗ Belo Horizonte, MG

https://orcid.org/0000-0003-0339-1767

F. Pierezan

https://orcid.org/0000-0002-3319-8784

H.P. Tinoco

https://orcid.org/0000-0003-4270-5503

A.R. Hoffmann

College Station ˗ Texas A&M University ˗ Texas, USATexas A&M UniversityUSATexas, USACollege Station ˗ Texas A&M University ˗ Texas, USA

https://orcid.org/0000-0001-6148-2531

R. Torres Neto

Autônomo ˗ Botucatu, SPAutônomo ˗ Botucatu, SPBrazilBotucatu, SP, BrazilAutônomo ˗ Botucatu, SP

https://orcid.org/0000-0001-8523-8541

R.L. Amorim

Faculdade de Medicina Veterinária e Zootecnia ˗ Universidade Estadual Paulista ˗ Botucatu, SPUniversidade Estadual PaulistaBrazilBotucatu, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia ˗ Universidade Estadual Paulista ˗ Botucatu, SP

https://orcid.org/0000-0002-8653-7938

T.A. Paixão

Instituto de Ciências Biológicas ˗ Universidade Federal de Minas Gerais ˗ Belo Horizonte, MGUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilInstituto de Ciências Biológicas ˗ Universidade Federal de Minas Gerais ˗ Belo Horizonte, MG

https://orcid.org/0000-0001-8284-9902

R.L. Santos ^**Autor para correspondência (corresponding author) E-mail: rsantos@vet.ufmg.br

https://orcid.org/0000-0002-4830-0470

*Autor para correspondência (corresponding author) E-mail: rsantos@vet.ufmg.br

SCIMAGO INSTITUTIONS RANKINGS

College Station ˗ Texas A&M University ˗ Texas, USATexas A&M UniversityUSATexas, USACollege Station ˗ Texas A&M University ˗ Texas, USA

Autônomo ˗ Botucatu, SPAutônomo ˗ Botucatu, SPBrazilBotucatu, SP, BrazilAutônomo ˗ Botucatu, SP

Figuras | Tabelas

Figuras (3)
Tabelas (1)

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Figure 1
Kidney, lymphoma in a bush dog (Speothos venaticus). Top: left and right kidneys with nodules protruding on the organ surface. Bottom: cut surface of nodular lesions with a whitish solid tissue with extensive areas of hemorrhage.

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Figure 2
Kidney, lymphoma in a bush dog (Speothos venaticus). (A) densely cellular and infiltrative round cell tumor HE, Bar = 100μm; (B) Solid sheets of neoplastic cells characterized by larger and pleomorphic cells, with abundant eosinophilic cytoplasm and marked anisokaryosis, infiltrated with abundant smaller cells with scant cytoplasm and central round nuclei that resemble well differentiated lymphocytes. HE, Bar = 50μm.

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Figure 3
Kidney, lymphoma in a bush dog (Speothos venaticus). Immunohistochemistry panel: (A) CD3 positive cells - mostly smaller cells with lymphocyte morphology, corresponding to approximately 40% of the cell population within the neoplasm; (B) CD45 - the population of large and pleomorphic neoplastic cells stained weakly but homogeneously for CD45; (C) Granzime B - pleomorphic neoplastic cells stained strongly for granzime B; (D) CD11d - pleomorphic neoplastic cells stained strongly for CD11d; (E) Iba1 - there was a considerable number of Iba1 positive cells with a histiocytic morphology scattered within the neoplasm. Neoplastic cells were negative for CD79a (F), PAX5 (G), and myeloperoxidase (H), with a few positive pre-existing non neoplastic cells that served as internal controls. IHC; the chromogen was 3,3-diaminobenzidine tetrachloride (DAB) for all sections, except Iba1 whose chromogen was 3-amino-9-ethylcarbazole (AEC). Sections were counterstained with Mayer’s hematoxylin.

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Table 1
Immunohistochemistry markers employed in this case

Figure 1 Kidney, lymphoma in a bush dog (Speothos venaticus). Top: left and right kidneys with nodules protruding on the organ surface. Bottom: cut surface of nodular lesions with a whitish solid tissue with extensive areas of hemorrhage.

Figure 2 Kidney, lymphoma in a bush dog (Speothos venaticus). (A) densely cellular and infiltrative round cell tumor HE, Bar = 100μm; (B) Solid sheets of neoplastic cells characterized by larger and pleomorphic cells, with abundant eosinophilic cytoplasm and marked anisokaryosis, infiltrated with abundant smaller cells with scant cytoplasm and central round nuclei that resemble well differentiated lymphocytes. HE, Bar = 50μm.

Figure 3 Kidney, lymphoma in a bush dog (Speothos venaticus). Immunohistochemistry panel: (A) CD3 positive cells - mostly smaller cells with lymphocyte morphology, corresponding to approximately 40% of the cell population within the neoplasm; (B) CD45 - the population of large and pleomorphic neoplastic cells stained weakly but homogeneously for CD45; (C) Granzime B - pleomorphic neoplastic cells stained strongly for granzime B; (D) CD11d - pleomorphic neoplastic cells stained strongly for CD11d; (E) Iba1 - there was a considerable number of Iba1 positive cells with a histiocytic morphology scattered within the neoplasm. Neoplastic cells were negative for CD79a (F), PAX5 (G), and myeloperoxidase (H), with a few positive pre-existing non neoplastic cells that served as internal controls. IHC; the chromogen was 3,3-diaminobenzidine tetrachloride (DAB) for all sections, except Iba1 whose chromogen was 3-amino-9-ethylcarbazole (AEC). Sections were counterstained with Mayer’s hematoxylin.

Table 1 Immunohistochemistry markers employed in this case

Marker	Type	Clone or catalogue number	Dilution	Supplier
CD3	Monoclonal	SP7	1:150	Fisher, Waltham, MA 02451, USA.
CD11d	Monoclonal	CA12.10C12	1:50	Dr. P. Moore, UC-Davis, Davis, CA 95616, USA.
CD20	Polyclonal, rabbit	Cat# E2560	1:1,000	Spring Biosciences, Pleasanton, CA 94566, USA.
CD45	Monoclonal	CA12.10C12	1:20	Dr. P. Moore, UC-Davis, Davis, CA 95616, USA.
CD79a	Monoclonal	HM47/A9	1:50	Biocare Medical, Pacheco, CA 94553, USA.
CD163	Polyclonal, rabbit	Cat# E18680	1:200	Spring Bioscience Corp., Pleasanton, CA 94566, USA.
Granzime B	Polyclonal, rabbit	Cat# E 2580	1:1,500	Spring Bioscience Corp., Pleasanton, CA 94566, USA.
Iba1	Polyclonal, rabbit	Cat# 019-19741	1:1,000	Wako, Richmond, VA 23285, USA.
Myeloperoxidase	Polyclonal, rabbit	Cat# IR511	Ready-to-use	Dako, Glostrup 2600, Denmark.
PAX5	Monoclonal	24/Pax-5	1:1,000	BD Biosciences, Lexington, KY 40502, USA.

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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[1] *Autor para correspondência (corresponding author) E-mail: rsantos@vet.ufmg.br