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Protein content analysis of the aqueous humor in congenital glaucoma

SUMMARY

Quantitative and qualitative analysis of the protein content of the aqueous humor from 20 primary congenital glaucoma and 18 congenital cataract individuais was performed.

Their aqueous humor was obteined during required surgical treatment. Bradford Method showed that the mean concentration of total protein content in the aqueous humor of patients with primary congenital glaucoma, was greater and statistically significant than the patients with congenital cataract.

Qualitative analysis was performed with Phast System Unit and consisted of SDS - PAGE 8 - 25% gradient and silver staining. All gels revealed the presence of one well defined 67 KD protein band (Albumin). High and low molecular weight proteins bands, were revealed and the statistical analysis was no significant.

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