Improvement of bone repair in diabetic rats subjected to ƛ780 nm low-level laser therapy

Nascimento, Marismar Fernandes do; Almeida, Bernadeth Moda de; Cunha, John Lennon Silva; Valois, Rafael Barreto Vieira; Pinheiro, Juliana Campos; Ribeiro, Maria Amália Gonzaga; Lima, Sonia Oliveira; Albuquerque-Júnior, Ricardo Luiz Cavalcanti de

doi:10.1590/S0102-865020150100000002

Acessibilidade / Reportar erro

Brasil

Acta Cirúrgica Brasileira

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Original Articles • Acta Cir. Bras. 30 (10) • Oct 2015 • https://doi.org/10.1590/S0102-865020150100000002 copy

Improvement of bone repair in diabetic rats subjected to ƛ780 nm low-level laser therapy¹ 1 Research performed at Laboratory of Morphology and Experimental Pathology, Science and Technology Institute, Universidade Tiradentes (UNIT), Aracaju-SE, Brazil.

Authorship SCIMAGO INSTITUTIONS RANKINGS

PURPOSE:

To investigate the effect of low-level laser therapy on bone healing in diabetic rats.

METHODS:

Bone cavities (19 mm diameter) were performed in the femur of 72 alloxan-induced diabetic rats, which were assigned into four groups: CTR (non-diabetic control), DBT (diabetic) CTRL (non-diabetic irradiated) and DBTL (diabetic irradiated). Low-level laser therapy was performed every 48h for seven days. Animals were euthanized at seven, 18 and 30 days. Alkaline phosphatase serum levels and bone repair were analyzed.

RESULTS:

Low-level laser therapy significantly increased alkaline phosphatase in at seven and 18 days (p<0.001), and improved bone healing at seven (p<0.01), 18 (p<0.05) and 30 (p<0.01) in diabetic animals. In addition, bone healing in irradiated diabetic group was statistically similar to control group at 30 days (p>0.05).

CONCLUSION:

Low-level laser therapy increased the serum levels of alkaline phosphatase and improved bone healing in alloxan-induced diabetic rats.

Laser Therapy; Low-Level; Diabetes Mellitus; Experimental; Osteogenesis; Alkaline Phosphatase; Rats

Groups (n=18)^a	Glycemic status	Energy density of low-level laser
CTR	normoglycemic (non-diabetic)	0 j/cm^{2 b}
DBT	hyperglycemic (diabetic)	0 j/cm^{2 b}
CTRL	normoglycemic (non-diabetic)	17,5 j/cm²2. American Diabetes Association. Diagnosis and classification of Diabetes Mellitus. Diabetes Care. 2010 Jan;33(Suppl 1):S62-S9. doi: 10.2337/dc10-S062. https://doi.org/10.2337/dc10-S062...
DBLT	hyperglycemic (diabetic)	17,5 j/cm²2. American Diabetes Association. Diagnosis and classification of Diabetes Mellitus. Diabetes Care. 2010 Jan;33(Suppl 1):S62-S9. doi: 10.2337/dc10-S062. https://doi.org/10.2337/dc10-S062...

Parameters of irradiation	Protocol of low-level laser therapy
Emission	Continuous mode
Wavelength	780±10 (infrared)
Light emitter active medium	GaAlAs
Power output	70 mW
Spot size	2.3 mm
Area of the laser beam	0.04 cm²2. American Diabetes Association. Diagnosis and classification of Diabetes Mellitus. Diabetes Care. 2010 Jan;33(Suppl 1):S62-S9. doi: 10.2337/dc10-S062. https://doi.org/10.2337/dc10-S062...
Power density	1,75 W/cm²2. American Diabetes Association. Diagnosis and classification of Diabetes Mellitus. Diabetes Care. 2010 Jan;33(Suppl 1):S62-S9. doi: 10.2337/dc10-S062. https://doi.org/10.2337/dc10-S062...
Energy density/session	17,5 J/cm²2. American Diabetes Association. Diagnosis and classification of Diabetes Mellitus. Diabetes Care. 2010 Jan;33(Suppl 1):S62-S9. doi: 10.2337/dc10-S062. https://doi.org/10.2337/dc10-S062...
Irradiation time/session	10 s
Total irradiation time	40 s
Total energy/session	0,7 J
Beam divergence perpendicular to the junction	17º
Tip angle	50º

Experimental groups	Intensity of the inflammatory reaction
Experimental groups	7 days	18 days	30 days
CTR	1.25 ± 0.40 ^a	0.41 ± 0.50 ^a	0.12 ± 0.33 ^a
CTRL	0.79 ± 0.31 ^b	0.25 ± 0.44 ^a	0.08 ± 0.28 ^a
DBT	2.70 ± 0.46 ^c	0.98 ± 0.33 ^b	0.29 ± 0.46 ^a
DBTL	2.45 ± 0.65 ^c	0.50 ± 0.51 ^a	0.20 ± 0.41 ^a
p values*	0.0001	0.001	0.245

Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia https://actacirbras.com.br/ - São Paulo - SP - Brazil
E-mail: actacirbras@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] Correspondence: Ricardo Luiz Cavalcanti de Albuquerque-Júnior Laboratório de Morfologia e Patologia Experimental Instituto de Tecnologia e Pesquisa, Universidade Tiradentes Avenida Murilo Dantas, 300 49032-490 Aracaju - SE Brasil Tel.: (55 79)3218-2190/ramal 2615ricardo.patologia@uol.com.br

Brasil

Brasil

Improvement of bone repair in diabetic rats subjected to ƛ780 nm low-level laser therapy1 1 Research performed at Laboratory of Morphology and Experimental Pathology, Science and Technology Institute, Universidade Tiradentes (UNIT), Aracaju-SE, Brazil.

PURPOSE:

METHODS:

RESULTS:

CONCLUSION:

Improvement of bone repair in diabetic rats subjected to ƛ780 nm low-level laser therapy¹ 1 Research performed at Laboratory of Morphology and Experimental Pathology, Science and Technology Institute, Universidade Tiradentes (UNIT), Aracaju-SE, Brazil.