Effects of maternal temperament on uterine and umbilical blood flow, expression of angiogenic proteins, and fetal growth in pregnant ossimi sheep

Elmetwally, Mohammed; Adlan, Fatma; Hendam, Basma; Samy, Alaa; Rezk, Shaymaa; Lasheen, Samah; Mohram, Heba; Shalapy, Sara; Fahmy, Reham; Montgomery, Amy Jo; Lenis, Yasser Y; Mostagir, Amira

doi:10.1590/1984-3143-AR2024-0125

person Mohammed Elmetwally *

Conceptualization · Methodology · Validation · Formal analysis · Investigation · Data curation · Writing - original draft

schoolDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptschoolCenter for Reproductive Biotechnology Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptschoolMansoura Veterinary Teaching Hospital, Mansoura University, Mansoura, Egypt

0000-0001-7486-7756

person Fatma Adlan

Conceptualization · Validation · Formal analysis · Writing - review & editing

schoolDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptschoolCenter for Reproductive Biotechnology Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0009-0007-7882-8317

person Basma Hendam

Data curation · Formal analysis · Writing - review & editing · Writing original manuscript & editing

schoolDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0000-0001-7420-6704

person Alaa Samy

Data curation · Formal analysis · Methodology · Writing – original draft

schoolDepartment of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0000-0001-5332-9828

person Shaymaa Rezk

Methodology · Writing – original draft

schoolDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0000-0002-8046-4299

person Samah Lasheen

Methodology · Writing – original draft

schoolDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0000-0003-3633-8049

person Heba Mohram

Data curation · Formal analysis · Methodology · Writing – original draft

schoolDepartment of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0009-0002-5738-8619

person Sara Shalapy

Data curation · Formal analysis · Methodology · Writing – original draft

schoolDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptschoolCenter for Reproductive Biotechnology Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0000-0002-8094-986X

person Reham Fahmy

Data curation · Formal analysis

schoolOncology Center, Faculty of Medicine; Mansoura University, Mansoura, Egypt

0009-0007-0275-0322

person Amy Jo Montgomery

Funding acquisition · Data curation · Formal analysis · Methodology · Writing – original draft

schoolSchool of Medicine, St. George’s University, West Indies, Grenada

0000-0001-9989-1381

person Yasser Y Lenis

Funding acquisition · Data curation · Writing - original draft

schoolResearch Group OHVRI, School of Veterinary Medicine, Faculty of Agrarian Science, Universidad de Antioquia, Medellín, Colombia

0000-0002-4976-9916

person Amira Mostagir

Data curation · Formal analysis · Methodology · Writing – original draft

schoolDepartment of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptschoolCenter for Reproductive Biotechnology Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

0009-0004-0300-5381

person*Corresponding author:
emailmmetwally@mans.edu.eg

Conflicts of Interest:

The authors have no conflict of interest to declare.

Editor-in-Chief:

Carlos Eduardo Ambrósio.

Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Center for Reproductive Biotechnology Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Mansoura Veterinary Teaching Hospital, Mansoura University, Mansoura, Egypt

Department of Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

Oncology Center, Faculty of Medicine; Mansoura University, Mansoura, Egypt

School of Medicine, St. George’s University, West Indies, Grenada

Research Group OHVRI, School of Veterinary Medicine, Faculty of Agrarian Science, Universidad de Antioquia, Medellín, Colombia

Feed ingredients (%)
Soybean meal	6
Cotton seed cake	16
Wheat bran	24
Yellow corn	51
Ground limestone	1.7
Common salt	0.8
Mineral-vitamin premix	0.5
Feed composition (%)
Crude protein	14
Ether extract	4
Total digestible nutrients	68
Fiber	8
Ash	6

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Accession no	Product length
NOS3	CAAGGTGACTTCCCAGAGACT	GAGAGAGGCGAGGGGAATCT	NM_001129901.1	173
VEGF	CTTGCCTTGCTGCTCTACCT	GCCCACAGGGATTTTCTTGC	AF071015.1	397
HIF-1α	ACAGCAAGAACCTGTTGCCT	TCAAGGCAGGGGAGCATACA	XM_027973633.2	151
β-actin	TGTTAGCTGCGTTACACCCT	GTCAAAGTCCTCGGCCACAT	NM_001009784.3	180

Time (wks)	TAMV-UTA-MR	TAMV-UTA-LR	PI- UTA-MR	PI- UTA-LR	RI- UTA-MR	RI- UTA-LR
2	11.36±3.33 ^a	9.27±1.75 ^b	7.79±0.61 ^a	5.19±0.6 ^b	0.90±0.12 ^a	0.77±0.08 ^b
4	14.61±3.37 ^b	19.77±1.10 ^a	6.59±0.32 ^a	3.40±0.8 ^b	0.86±0.06 ^a	0.70±0.06 ^b
6	17.3±4.07 ^b	26.95±3.40 ^a	4.61±0.82 ^a	3.39±0.88 ^b	0.83±0.01 ^a	0.70±0.05 ^b
8	23.68±4.20 ^b	31.44±3.31 ^a	3.30±0.68 ^a	2.28±0.80 ^b	0.81±0.03 ^a	0.81±0.04 ^b
10	29.17±4.25 ^b	37.8±2.01 ^a	3.10±0.38 ^a	1.45±0.39 ^b	0.81±0.06 ^a	0.64±0.07 ^b
12	32.28±3.27 ^b	40.22±1.73 ^a	2.34±0.50 ^a	1.31±0.17 ^b	0.81±0.08 ^a	0.66±0.03 ^b
14	36.72±4.02 ^b	46.12±2.40 ^a	0.98±0.40 ^b	0.91±0.19	0.72±0.05 ^a	0.57±0.04 ^b
16	42.63±6.09 ^b	54.73±2.30 ^a	0.95±0.27	0.97±0.24	0.77±0.02 ^a	0.63±0.02 ^b
18	43.91±5.24 ^b	56.86±3.77 ^a	1.23±0.10	0.98±0.15	0.77±0.02 ^a	0.63±0.04 ^b
20	48.61±4.38 ^b	60.43±3.31 ^a	2.65±0.17 ^a	1.66±0.09 ^b	1.00±0.01 ^a	0.78±0.03 ^b

Time (wks)	TAMV-um-MR	TAMV-um-LR	PI-MR	PI-LR	RI-MR	RI-LR
10	15.61±2.37 ^b	22.34±2.18^a	2.12±0.35	2.12±0.41	1.00±0.06	1.00±0.07
12	18.43±2.56 ^b	24.91±2.27 ^a	2.22±0.11^a	1.42±0.29 ^b	0.98±0.06 ^a	0.78±0.04 ^b
14	22.14±2.41 ^b	27.18±3.17	2.68±0.14 ^a	1.41±0.09 ^b	0.96±0.02 ^a	0.83±0.09 ^b
16	22.85±2.41 ^b	28.46±3.15^a	1.54±0.08	1.49±0.05	0.82±0.4 ^a	0.77±0.04 ^b
18	28.03±2.61 ^b	30.76±2.03 ^a	1.12±0.11 ^b	1.44±0.07 ^a	0.97±0.11 ^a	0.91±0.05 ^b
20	27.80±4.94	25.94±5.46	1.59±0.39 ^b	1.80±0.13 ^a	0.92±0.13 ^b	0.99±0.13 ^a

Time (wks)	AV value (MR)	AV value (LR)
2	1.55±0.12	1.6±0.16
4	2.25±0.32^b	2.77±0.45^a
6	4.15±1.6^b	4.97±1.30^a
8	5.73±1.12^b	6.74±1.44^a

Time (wks)	FTD values (MR)	FCHD values (LR)
6	3.45±0.6	3.6±0.15
8	3.47±0.8^b	3.9±0.22^a
10	3.61±0.48^b	4.5±0.33^a
12	3.68±0.55^b	4.61±0.31^a
14	3.87±0.33^b	4.67±0.23^a
16	4.05±0.31^b	4.78±0.8^a
18	4.27±0.21^b	4.82±0.9^a

Time (wks)	UMD values (MR)	UMD values (LR)
6	2.18±0.20^a	1.85±0.04^b
8	2.31±0.15 ^a	1.93±0.08 ^b
10	2.37±0.08	2.12±0.19
12	2.4±0.08	2.3±0.13
14	2.45±0.09	2.38±0.08
16	2.48±0.06	2.44±0.09
18	2.50±0.09	2.55±0.11

Time (wks)	MCL value (MR)	MCL value (LR)
6	2.34±0.22	2.7±0.13
8	2.49±0.32 ^b	2.73±0.22 ^a
10	2.5±0.81 ^b	3.04±0.15 ^a
12	2.53±0.27 ^b	3.24±0.31 ^a
14	2.66±0.15 ^b	3.26±0.34 ^a
16	2.73±0.4 ^b	3.36±0.30 ^a
18	2.75±0.33 ^b	3.4±0.31 ^a

items	More reactive (MR)	Less reactive (LR)	P-value
Gestational length	148.2 ± 1.49^b	153.7 ± 1.54^a	P > 0.05
Fetal weight	3.64 ± 1.24 ^b	3.85 ± 1.35 ^a	P > 0.05
Fetal number	1.57 ± 0.65 ^b	1.65 ± 0.50 ^a	P > 0.05

Gene	LR	MR	P-value
NOS3	1.00 ± 0.00	0.3 ± 0.0.13 ∗∗	0.03
VEGF	1.00 ± 0.00	0.19 ± 0.09∗∗	0.0001
HIF-1α	1.00 ± 0.00	0.25± 0.41∗∗	0.001

[1] NOS3 endothelial nitric acid synthase, VEGF vascular endothelial growth factor, HIF hypoxia inducing factor 1 alpha.