In vitro antibacterial-antibiofilm effect of <i>Hypericum atomarium</i> Boiss and chemical composition

Önem, Ebru; Özaydın, Ayşe Gül; Sarısu, Hasan Cumhur

doi:10.1590/s2175-97902023e20412

Acessibilidade / Reportar erro

Brasil

Brazilian Journal of Pharmaceutical Sciences

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Article • Braz. J. Pharm. Sci. 59 • 2023 • https://doi.org/10.1590/s2175-97902023e20412 copy

In vitro antibacterial-antibiofilm effect of Hypericum atomarium Boiss and chemical composition

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Treatment with plant is considered an effective option against increased antibiotic resistance. In this study antibiofilm activity of methanol (CH₃OH), chloroform (CHCl₃), ethyl acetate (EtOAc) and water (H₂O) extracts of Hypericum atomarium Boiss. which is member of Hypericum genus was evaluated in Pseudomonas aeruginosa PAO1 and antibacterial performance against Gram (+) and Gram (-) strains and also bioactive compounds of extract were analysed using by HPLC and GC-MS. According to antibacterial activity test results the extracts were effective all Gram (+) bacteria and Gram (-) Chromobacterium violaceum (MICs ranging from 0.42 μg/ml to 4.3 mg). Inhibition effect of biofilm formation was found to be different rate in extracts (methanol-63%, chloroform-52%). The major flavonoids were detected (−)-epicatechin (2388.93 μg/ml) and (+)-catechin (788.94 μg/ml). The main phenolic acids were appeared as caffeic acid 277.34 μg/ml and chlorogenic acid 261.79 μg/ml. And according to GC results α-pinene was found main compound for three solvent extracts methanol, chloroform and ethyl acetate 67.05, 62.69, 49.28% rate respectively.

Keywords:
Antibacterial activity; Hypericaceae; Phenolic acids; PAO1; Quorum sensing

No	Name of compound	rt (min)	EtOAc (%)	CHCl3 (%)
1	n-Decane	5.119	10.27	*
2	n-Nonane	5.127	*	17.03
3	α-pinene	6.150	49.28	62.69
4	2.6-Dimethyloctane	7.336	*	8.71
5	2-Methyleicosane	7.340	15.32	*
6	3-Methylnonane	7.365	*	*
7	Cyclohexene	9.710	15.32	*
8	Hexane 3,3 dimetyl	11.409	*	5.41
9	3-Methyoctane	11.456	*	*
10	E-3-phenyl-2propenal	23.385	21.86	*
11	3-Nonen-1-yne	32.828	*	6.16

Compounds	µg/g
*Phenolics acids*
gallic acid	17.0±2.4
protocatechuic acid	*
p-hydroxy benzoic acid	253.61±16.0
chlorogenic acid	261.79±8.1
caffeic acid	277.34±12.7
syringic acid	*
vanillin	*
p-coumaric acid	13.41±0.5
ferulic acid	14.69±1.3
sinapinic acid	*
benzoic acid	*
o-coumaric acid	516.33±20.9
rosmarinic acid	603.36±21.3
cinnamic acid	7.33±0.2
*Flavonoids*
rutin	312.55±7.3
catechin	788.94±22.2
hesperidin	*
epicatechin	2388.93±58.1
eriodictiol	3.64±0.4
quercetin	352.24±12.1
luteolin	25.06±0.03
kaempferol	*
apigenin	*

Sample/Concentration(w/v)	B. subtilis 6633				B. cereus 11778
	1:1	1:2	1:4	1:8	1:1	1:2	1:4	1:8
H₂O	14.7^b	13.7^c	12.7^c	12.7^c	20.7^a	18.7^c	16.7^b	14.0^c
CH₃OH	20.7^a	20.0^ab	19.0^ab	19.0^ab	20.7^a	20.7^a	19.7^a	19.0^b
CHCl₃	21.0^a	21.0^a	20.7^a	20.7^a	21.0^a	20.0^b	20.0^a	19.0^b
EtOAc	21.0^a	21.0^a	18.7^b	18.0^b	20.7^a	20.7^a	20.0^a	20.0^a
α-pinene	11.7^c	ne	ne	ne	11.7^c	ne	ne	ne
(-)-epicatechin	ne	ne	ne	ne	ne	ne	ne	ne
(±)-catechin	ne	ne	ne	ne	ne	ne	ne	ne
CN (40μg/ml)	19.3^a	19.3^b	19.3^ab	19.3^ab	13.3^b	13.3^d	13.3^c	13.3^d
	***	***	***	***	***	***	***	***
	C. violaceum 12472				E. faecalis 29212
H₂O	10.7^cd	10.0^b	9.0^b	8.0^c	15.7^ab	13.0^c	11.7^c	9.0^d
CH₃OH	17.3^a	15.3^a	13.0^a	10.3^b	16.7^a	16.7^a	15.7^a	13.7^b
CHCl₃	12.0^c	10.0^b	7.7^b	7.7^c	13.7^c	13.7^c	13.0^b	12.0^c
EtOAc	9.0^d	8.7^b	ne	ne	15.0^b	13.7^c	13.7^b	12.7^c
α-pinene	ne	ne	ne	ne	9.3^d	ne	ne	ne
(-)-epicatechin	ne	ne	ne	ne	ne	ne	ne	ne
(±)-catechin	ne	ne	ne	ne	ne	ne	ne	ne
CN (40μg/ml)	14.3^b	14.3^a	14.3^a	14.3^a	15.7^ab	15.7^b	15.67^a	15.7^a
	***	***	***	***	***	***	***	***
	L. monocytogenes 7644				MRSA 43300
H₂O	12.7^d	12.7^c	10.7^b	9.0^c	17.0^b	14.0^c	12.7^b	9.0^c
CH₃OH	18.7^b	19.0^a	18.0^a	18.0^a	18.7^a	18.0^b	18.7^a	18.0^a
CHCl₃	19.7^a	18.0^ab	16.7^a	16.7^ab	18.7^a	18.0^b	17.0^a	17.0^ab
EtOAc	17.7^c	17.0^b	16.7^a	15.7^b	19.3^a	19.3^a	18.7^a	17.7^a
α-pinene	ne	ne	ne	ne	10.3^d	ne	ne	ne
(-)-epicatechin	ne	ne	ne	ne	ne	ne	ne	ne
(±)-catechin	ne	ne	ne	ne	ne	ne	ne	ne
CN (40μg/ml)	18.0^bc	18.0^ab	18.0^a	18.0^a	14.3^c	14.3^c	14.3^b	14.3^b
	***	***	***	***	***	***	***	**
	S. aureus 25923
H₂O	15.7c	13.7c	11.3c	9.0c
CH₃OH	19.0a	19.0a	18.0a	18.0a
CHCl₃	17.7b	17.7b	17.0a	16.7a
EtOAc	18.7ab	18.0ab	17.0a	17.0a
α-pinene	16.3c	ne	ne	ne
(-)-epicatechin	ne	ne	ne	ne
(±)-catechin	ne	ne	ne	ne
CN (40μg/ml)	14.3^d	14.3^c	14.3^b	14.3^b
	***	***	***	***

Extract	Yield (%)	*S.aureus*	*MRSA*	*B. subtilis*	*B. cereus*	*E. faecalis*	*C. violaceum*	*L. monocytogenes*
CHCl₃	7.94	53	53	0.42	0.42	107	1730	431
CH₃OH	18.12	265	265	1.03	1.03	66	4300	2130
EtOAc	8.16	3.4	3.4	0.43	0.43	28	1775	443
H₂O	15.78	1225	1225	2.39	2.39	153	2450	1225
Gentamicin	-	1250	1250	1250	1250	1250	6250	6250

Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Av. Prof. Lineu Prestes, n. 580, 05508-000 S. Paulo/SP Brasil, Tel.: (55 11) 3091-3824 - São Paulo - SP - Brazil
E-mail: bjps@usp.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Correspondence: E. Onem. Department of Pharmaceutical Microbiology. Faculty of Pharmacy. Süleyman Demirel University. 32100 Isparta, Turkey. E-mail: ebruonem@sdu.edu.tr.