This work aimed to determine a right substrate for acclimatization of pineapple 'Gomo de Mel' plantlets and its anatomical characterization during this process. Rooting of the shoots was done in MS medium supplemented with 30 g L-1 sucrose and 6 g L-1 agar. Cultures were maintained in growth room at 25±1º C, 36µmol m-2 s-1 during 16 hours. After 60 days, rooted shoots were removed from the bottles and distributed in tubes containing the following treatments: 1) A+X+H (sand, fern tree fiber and humus) (1: 1: 1); 2) commercial substrate Plantmax®; 3) vermiculite and 4) 1:1 combination of Plantmax® + Vermiculite. The anatomical characteristics were evaluated when the seedlings were still in vitro and in the 7th; 15th; 30th and 60th days of acclimatization. The transition leaves were also characterized. The experiment was installed in a randomized completely design, with four treatments and four replicates containing five plants. Higher lengths of roots, fresh and dry mass of roots and roots were observed with the use of sand + fern tree fiber + humus. Leaf number, fresh mass of roots and dry mass of roots did not show differences between substrates. Regarding the anatomical characteristics, the use of vermiculite substrate in the 60 days period of acclimatization promoted the highest thicknesses of the limb tissues.
Ananas comosus; plant anatomy; tissue culture
