The objective of this work was to evaluate the germination of pollen grain and pollen tube length of banana diploids (M53, 8987-01 e 9197-03, Calcutta, Lidi and 86B79-12). Five concentrations of sucrose (0, 5, 10, 15 and 20%) and six concentrations of boric acid (0, 100, 200, 300, 400 and 500 mg L-1) were used, in a completely randomized experimental design, with four replicates. The pollen was distributed in Petri dishes containing the following culture medium: 1.27 mM Ca(NO3)2.2H2O, 0.87 mM MgSO4.7H2O, 0.99 mM KNO3, and 0.7% agar; pH adjusted to 7.0, varying the sucrose and boric acid concentrations. The evaluations were performed 24 hours after the distribution of the pollen in the culture medium. The standard culture medium for germination of pollen grains with 15% sucrose provided the highest germination percentage for the banana diploids. The concentration of boric acid added to the culture medium for pollen grain germination of diploid banana is dependent on the genotype.
Musa acuminata; diploids; germination; pollen tube
