Effects of Viscum coloratum (Kom.) Nakai f. Lutescens Kitag polysaccharide on fertility, longevity and antioxidant capacity of Drosophila melanogaster

YE, Wenbin; ZHANG, Long; WANG, Duli; HE, Yufeng; LI, Na; JIANG, Jing; MA, Yingli

doi:10.1590/fst.09721

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Original Article • Food Sci. Technol (Campinas) 42 • 2022 • https://doi.org/10.1590/fst.09721 copy

Effects of Viscum coloratum (Kom.) Nakai f. Lutescens Kitag polysaccharide on fertility, longevity and antioxidant capacity of Drosophila melanogaster

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The purpose of this study was to investigate the effects of Viscum coloratum (Kom.) Nakai f. Lutescens Kitag polysaccharide on fertility, longevity and antioxidant capacity of Drosophila melanogaster. The Drosophila melanogaster were randomly divided into five groups. Four generations of Drosophila melanogaster were cultured continuously in basic medium with or without polysaccharides (400, 200, 100 and 50 mg/L). The changes in fertility, lifespan and male-to-female ratio from F₁ to F₄ generation. The survival time under acute oxidative damage caused by paraquat and hydrogen peroxide (H₂O₂) and changes in superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) content were determined as well. High dose of V. coloratum polysaccharide could significantly improve the fertility and lifespans of Drosophila melanogaster from F₁ to F₄ generation, prolong the survival time under acute oxidative damage. Moreover, the MDA content was decreased sharply, while the activities of SOD and CAT were increased in high group. However, the male-to-female ratio was overall close to 1:1 for F1 to F4 generations among the four groups. V. coloratum polysaccharide improved the antioxidant capacity and vitality of Drosophila melanogaster and promoted their reproductive capacity in a dose-dependent manner.

Keywords:
Viscum coloratum (Kom.) Nakai f. Lutescens Kitag polysaccharide; Drosophila melanogaster; fertility; longevity; antioxidant capacity

Polysaccharide concentration (mg/L)	F₁		F₂		F₃		F₄
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂	♀	♂
CK (0)	298±3.13^aA	299±2.62^aA	274±1.53^aA	270±1.72^aA	244±1.92^aA	233±0.62^aA	235±3.13^aA	228±2.62^aA
50	309±2.06^aA	316±2.80^aA	295±1.43^aA	288±2.18^aA	259±1.84^aA	253±1.53^aA	251±2.06^aA	244±2.81^aA
100	331±2.61^bA	334±2.65^bA	324±1.47^bA	314±1.55^bA	302±1.53^bB	296±1.68^bB	296±2.61^bB	293±2.65^bB
200	437±2.44^cB	440±2.28^cB	431±1.65^cB	428±1.37^cB	407±2.43^cC	402±0.53^cC	409±2.44^cC	404±2.28^cC
400	487±4.18^dC	491±3.25^dC	480±3.58^dC	475±2.45^dC	497±2.8^1dD	491±2.14^dD	474±4.18^dD	477±3.25^dD

Polysaccharide concentration (mg/L)	F₁	F₂	F₃	F₄	Total
Polysaccharide concentration (mg/L)	F₁	F₂	F₃	F₄	♀	♂	♀+♂
CK (0)	597±3.70^aA	544±3.63^aA	477±3.21^aA	463±3.67^aA	1051±3.27^aA	1030±3.37^aA	2081±7.80 ^aA
50	625±3.21^bA	583±3.01 ^bA	412±3.62^bA	495±3.61^bA	1114±3.67^bB	1101±4.77 ^bB	2215±7.58 ^bB
100	665±3.61^cB	638±3.19^cB	598±3.57^cB	589±3.65^cB	1253±5.08^cB	1237±5.21^cC	2490±7.81^cC
200	877±2.80^dC	859±2.60^dC	809±3.50^dC	813±3.62^dC	1684±4.50^dC	1674±4.54^dD	3358±8.50^dD
400	978±2.97^eD	955±3.20 ^eD	988±2.99 ^eD	951±2.81^eD	1938±2.84^eD	1934±4.81^eE	3872±7.70^eE

Polysaccharide concentration (mg/L)	F₁	F₂	F₃	F₄	Mean
CK (0)	0.997	1.014	1.047	1.030	1.022
50	0.978	1.024	1.024	1.029	1.014
100	0.991	1.031	1.020	1.010	1.013
200	0.993	1.007	1.012	1.012	1.006
400	0.992	1.011	1.012	0.994	1.002

Polysaccharide concentration (mg/L)	F1		F2		F3		F4
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂	♀	♂
CK (0)	50.89±0.36^aA	51.61±0.46^aA	56.94±0.44^aA	51.82±0.26^aA	54.79±0.26^aA	54.90±0.70^aA	50.54±0.64^aA	54.65±0.94^aA
50	51.88±0.58^aA	53.92±0.30^aA	57.62±0.72^aA	54.54±0.28^aA	56.81±0.35^aA	52.10±0.34^aA	53.64±0.32^aA	59.90±0.43^aA
100	70.91±0.32^bB	70.76±0.28^bB	69.81±0.43^bB	66.89±0.32^bB	70.94±0.26^bB	63.85±0.42^bB	65.91±0.34^bB	63.88±0.34^bB
200	74.63±0.40^cB	76.04±0.48^cB	78.01±0.72^cB	78.85±0.70^cB	73.94±0.19^cB	70.95±0.32^cB	73.84±0.41^cB	78.96±0.32^cB
400	79.73±0.72^dC	78.98±0.52^dC	81.01±0.32^dC	81.88±1.00^dC	82.91±0.30^dC	79.11±0.32^dC	80.18±0.43^dC	83.24±0.63^dC

Polysaccharide concentration (mg/L)	Accumulated lifespan of (F₁- F₄)		Average lifespan of (F₁- F₄)		The longest lifespan of (F₁- F₄)
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂
CK(0)	213.16±4.11^aA	213.01±4.79^aA	53.29±0.72^aA	53.25±0.65^aA	57.81±0.55^aA	58.74±0.39^aA
50	219.95±3.79^bA	220.46±2.30^bA	54.99±0.68 ^aA	55.12±0.39^aA	64.40±0.45^bB	66.76±0.28^bB
100	277.54±3.09^cB	265.38±2.82^cB	69.39±0.91^bB	66.35±0.79^bB	72.80±0.49^cC	73.73±0.43^cC
200	300.42±2.45^dC	304.80±0.75^dC	75.11±0.67^cC	76.20±0.50 ^cC	80.72±0.76^dD	77.39±0.47^dD
400	323.83±2.63^dD	323.21±0.36^dD	80.96±0.30^dD	80.80±0.49^dD	85.52±0.83^eE	84.38±0.89^eE

Polysaccharide concentration (mg/L)	F₁			F₁			F₃			F₄
Polysaccharide concentration (mg/L)	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time
CK (0)	25.12±1.04^aA	23.12±0.84^aA	38.17±0.76^aA	24.92±1.14^aA	23.39±0.54^aA	38.49±0.96^aA	25.32±1.64^aA	23.86±0.77^aA	38.21±0.19^aA	25.41±1.09^aA	23.36±0.97^aA	38.11±0.14^aA
50	27.44±1.76^aA	24.54±0.91^aA	39.32±0.59^aA	27.64±0.26^bB	24.94±0.31^aA	39.92±0.99^aA	27.86±0.39^bB	25.04±0.86^aA	40.03±0.91^aA	28.16±0.77^bB	25.64±0.38^aA	40.63±0.29^aA
100	28.28±1.74^bA	25.25±0.24^bA	39.36±0.66^aA	28.78±0.14^bB	25.55±0.26^bA	40.56±0.96^bA	29.15±0.63^cB	25.63±0.56^aA	40.96±0.66^bA	29.75±0.58^bB	25.83±0.57^aA	41.69±0.87^bA
200	30.25±1.07^cA	26.18±0.55^bA	40.25±1.09^bA	30.65±0.27^cB	26.54±0.39^bA	40.95±0.89^bA	31.55±0.87^dC	26.89±0.46^bA	41.15±0.94^bA	31.65±0.83^cC	27.19±0.34^bA	41.65±0.44^bA
400	31.12±0.86^cA	27.39±0.38^cA	40.86±0.96^bA	31.29±0.39^cB	27.61±0.68^cB	41.69±0.76^cB	31.59±0.59^dC	28.32±0.49^cB	41.85±0.51^cB	31.87±0.39^cC	28.82±0.89^cB	42.15±0.91^cB

Polysaccharide concentration (mg/L)	F₁			F₂			F₃			F₄
Polysaccharide concentration (mg/L)	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time	Average survival time	Half survival time	The longest survival time
CK (0)	26.00±1.07^aA	23.89±0.96^aA	38.48±0.87^aA	25.19±1.26^aA	23.69±0.65^aA	38.79±0.36^aA	25.64±1.04^aA	24.03±0.64^aA	38.36±0.39^aA	25.56±0.69^aA	23.86±0.67^aA	38.61±0.74^aA
50	29.32±1.79^aA	25.16±1.01^aA	39.63±0.70^aA	28.01±0.37^bB	25.24±0.45^aA	40.22±0.39^aA	28.16±0.33^bB	25.22±0.56^aA	40.25±0.84^aA	28.33±0.67^bB	26.13±0.38^aA	41.13±0.89^aA
100	29.16±1.56^bA	25.99±0.36^bA	39.67±0.77^aA	29.15±0.28^bB	25.83±0.35^bA	40.87±0.84^bA	29.48±0.74^cB	25.80±0.44^aA	41.14±0.96^bA	29.95±0.48^bB	26.33±0.52^aA	42.19±0.67^bA
200	31.13±1.07^cA	26.90±0.67^bA	40.59±1.01^bA	30.92±0.47^cB	26.86±0.62^bA	41.25±0.19^bA	31.88±0.39^dC	27.06±0.18^bA	41.65±0.64^bA	31.85±0.33^cC	27.69±0.64^bA	42.35±0.49^bA
400	32.02±0.86^cA	28.09±0.38^cA	41.16±0.86^bA	31.57±0.52^cB	27.94±0.61^cB	41.91±0.71^cB	31.96±0.94^dC	28.58±0.99^cB	42.35±0.81^cB	32.07±0.69^cC	29.32±0.69^cB	43.95±1.11^cB

Polysaccharide concentration (mg/L)	F₁		F₂		F₃		F₄
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂	♀	♂
CK (0)	31.42±0.34^bA	35.92±0.41^bA	32.40±0.38^bA	35.90±0.45^bA	30.91±0.70^bA	35.31±0.67^bA	31.20±0.80^bA	34.75±0.74^bA
50	25.20±0.28^aA	28.70±0.54^aA	27.43±0.41^aA	30.93±0.91^aA	27.37±0.60^aA	30.93±0.67^aA	27.34±0.48^aA	30.58±0.47^aA
100	36.51±0.47^cB	40.01±0.54^cB	38.73±0.51^cB	42.23±0.62^cB	37.41±0.58^cB	40.93±0.50^cB	37.30±0.56^cB	40.63±0.44^cB
200	41.28±0.92^dB	44.78±0.81^dB	43.20±0.40^dB	47.73±0.58^dB	43.31±0.80^dB	47.78±0.86^dB	45.25±0.38^dB	48.47±0.91^dB
400	46.21±0.87^eB	49.86±0.98^eB	48.53±0.41^eB	51.68±1.03^eB	48.43±0.81^eB	52.05±0.70^eB	48.44±0.45^eB	50.39±0.60^eB

Polysaccharide concentration (mg/L)	F₁		F₂		F₃		F₄
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂	♀	♂
CK(0)	7.73±0.32^aA	8.61±0.89^aA	7.61±0.47^aA	7.83±0.68^aA	7.65±0.75^aA	8.70±0.91^aA	8.70±0.96^aA	10.00±1.12^aA
50	9.64±0.42^bA	10.88±0.41^bA	9.81±0.53^bA	11.05±0.74^bA	9.47±0.84^aA	10.77±0.90^aA	9.14±0.79^aA	10.87±0.65^aA
100	10.17±0.42^bA	11.41±0.35^bA	10.67±0.52^bA	11.91±0.66^bA	10.15±0.55^bA	11.45±1.17^bA	10.54±0.86^bA	11.90±1.01^bA
200	11.27±0.56^bA	12.51±0.60^bA	11.37±0.87^bA	12.59±0.95^bA	11.38±0.86^bA	12.62±0.89^bA	11.16±0.41^bA	12.56±0.73^bA
400	13.37±0.79^cA	14.60±0.90^cA	15.25±0.96^cA	15.49±0.66^cA	14.44±0.89^cA	15.55±1.06^cA	15.28±0.61^cA	15.48±0.82^cA

Polysaccharide concentration (mg/L)	F₁		F₂		F₂		F₃
Polysaccharide concentration (mg/L)	♀	♂	♀	♂	♀	♂	♀	♂
CK (0)	1.18±0.06^dB	1.36±0.03^dC	1.17±0.05^cB	1.35±0.04^dC	1.20±0.04^dC	1.45±0.04^dC	1.44±0.03^dC	1.71±0.06^dC
50	0.90±0.07^bA	1.10±0.05^bA	1.01±0.07^bA	1.09±0.05^bA	0.97±0.05^bA	1.15±0.04^bA	0.89±0.05^bA	1.07±0.07^bA
100	1.13±0.03^cB	1.21±0.03^cB	1.16±0.09^cB	1.19±0.07^cB	1.02±0.06^cA	1.20±0.04^cA	0.99±0.07^cA	1.17±0.06^cB
200	0.94±0.08^bA	1.02±0.06^bA	0.99±0.05^bA	1.05±0.05^bA	0.91±0.07^bA	1.11±0.09^bA	0.84±0.05^bA	1.02±0.07^bA
400	0.84±0.07^aA	0.91±0.09^aA	0.87±0.04^aA	0.96±0.04^aA	0.79±0.04^aA	0.88±0.06^aA	0.75±0.03^aA	0.89±0.07^aA

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