The influence of microbial bacterial proteins on metabolites in the chilled tan sheep meat

HU, Qianqian; ZHAO, Junyi; LUO, Ruiming; YOU, Liqin; ZHAO, Xiaoce; SU, Chunxia; ZHANG, Heyu

doi:10.1590/fst.24822

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Original Article • Food Sci. Technol 42 • 2022 • https://doi.org/10.1590/fst.24822 copy

The influence of microbial bacterial proteins on metabolites in the chilled tan sheep meat

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

This study aims to investigate experimentally the influence of microbial bacterial proteins on microbial metabolites in the chilled storage of Tan Sheep meat based on the surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) and gas chromatography-time of flight-mass spectrometry (GC-TOF MS). The correlation analysis of differentially abundant proteins and differential metabolites detected simultaneously in different storage periods has been carried out by heatmap analysis. The results show that 30 differential expressed metabolites were statistically significant. These metabolites were identified as potential spoilage biomarkers. Among of them, 25 differential expressed metabolites can be matched to KEGG metabolic pathways. cyano-amino-acid pathway, pentose phosphate pathway, histidine pathway, purine metabolic pathways, lanine, aspartic acid and glutamate pathway were highly active. Therefore, the differentially abundant proteins contents of microbiology in metabolism can lead to differential effects on metabolic pathways.

Keywords:
tan sheep meat; chilled storage; differentially abundant proteins; differential expressed metabolites; metabolic pathway

Mass-to-charge ratio	protein	ID	0 d	4 d	8 d	12 d
M7823_80	Growth-regulated alpha protein	A0A1V4JWG2	5.50% ± 0.21%	4.97% ± 0.18%	4.29% ± 0.18%	3.98% ± 0.17%
M8414_05	Necrosis-inducing phytophthora protein	A0A0W8CRK9	6.11% ± 0.27%	7.78% ± 0.24%	9.17% ± 0.3%	12.35% ± 0.13%
M10880_9	NADH-ubiquinone oxidoreductase 4L	P33602	6.01% ± 0.17%	8.91% ± 0.27%	9.75% ± 0.32%	10.43% ± 0.32%
M3130_58	Somatostatin-28	O46688	5.06% ± 0.19%	8.67% ± 0.23%	10.23% ± 0.46%	13.48% ± 0.21%
M5109_45	growth hormonereleasing factor	P07217	10.04% ± 0.32%	6.57% ± 0.19%	4.14% ± 0.16%	3.97% ± 0.12%
M6199_14	60S ribosomal protein L40	P14794	5.63% ± 0.22%	5.02% ± 0.14%	4.41% ± 0.17%	3.83% ± 0.15%
M7451_79	Antibacterial peptide	P85078	5.20% ± 0.16%	5.98% ± 0.19%	7.52% ± 0.21%	8.48% ± 0.22%
M7668_64	ATP synthase F(0) complex subunit C1	P68699	5.07% ± 0.17%	6.00% ± 0.11%	7.06% ± 0.39%	8.67% ± 0.17%
M7709_26	ATP synthase subunit	P0AB98	8.49% ± 0.24%	6.36% ± 0.17%	4.29% ± 0.13%	2.34% ± 0.16%
M8136_55	Apolipoprotein C-II	A0A3B4WGI6	5.25% ± 0.15%	6.75% ± 0.15%	7.93% ± 0.06%	8.78% ± 0.15%
M9074_86	prion protein	P31658	5.22% ± 0.13%	6.73% ± 0.14%	8.52% ± 0.12%	11.02% ± 0.17%
M11722_8	thioredoxin	P0AA25	6.01% ± 0.18%	5.14% ± 0.22%	4.75% ± 0.14%	3.99% ± 0.23%
M11870_3	β2microglobulin	Q6QAT4	5.50% ± 0.14%	4.92% ± 0.16%	4.19% ± 0.11%	3.86% ± 0.21%
M12826_1	40S ibosomal protein S26	Q54TL8	5.76% ± 0.16%	5.24% ± 0.21%	4.62% ± 0.08%	4.05% ± 0.19%
M34203_3	Apolipoprotein E	P23930	8.99% ± 0.21%	6.16% ± 0.24%	4.58% ± 0.15%	2.04% ± 0.17%
M38968_8	Annexin V	W5PSZ5	6.17% ± 0.16%	4.80% ± 0.16%	4.54% ± 0.04%	3.95% ± 0.23%

Peak	Similarity	Mass	MEAN 4-d	MEAN 0-d	VIP	P-VALUE	Q-VALUE	UP/DOWN
fumaric acid	955	245	0.049559603	0.148967592	1.19827	0.014203039	0.074211617	↓
D-Glyceric acid	940	189	0.452655273	0.068355163	2.09813	0.005578797	0.056722909	↑
phenylalanine 1	935	218	0.26421188	0.12826593	1.91066	0.000840454	0.028130129	↑
methionine 1	915	176	0.193085597	0.077284905	1.90914	0.000987958	0.028745611	↑
acetanilide 3	890	174	0.016042794	0.035838633	1.68182	0.011972667	0.070629781	↓
aspartic acid 1	886	232	0.078767479	0.022650788	1.87798	0.043086889	0.125072908	↑
Glucose-1-phosphate	879	217	0.199411461	0.302499655	1.65597	0.029773125	0.105513371	↓
D-(glycerol-phosphate)	869	299	0.350854272	0.217432157	1.58972	0.007540953	0.062742051	↑
Inosine-5'-monophosphate	853	169	0.145805445	0.482281053	1.65861	0.001637914	0.030241687	↓
asparagine 4	824	115	0.031170614	0.019762517	1.75942	0.007804362	0.063387527	↑
1,3-diaminopropane	817	174	9.80624E-09	1.53037E-08	1.65748	0.009422178	0.06676379	↓
lysine	792	156	0.05186895	0.019489268	1.99059	0.000609837	0.026683051	↑
2-Hydroxyvaleric acid	735	131	0.005920605	0.026883139	1.75419	0.04296625	0.124918656	↓
terephthalic acid	723	295	0.00816014	1.53037E-08	1.765	0.019103033	0.083963562	↑
5'-methylthioadenosine 1	710	236	0.004652561	0.007968691	1.8403	0.001655017	0.030266431	↓
Isomaltose 1	701	204	0.005889539	0.001352361	1.13718	3.88642E-05	0.007107354	↑

Peak	Similarity	Mass	MEAN 8d	MEAN 4d	VIP	P-VALUE	Q-VALUE	UP/DOWN
D-Glyceric acid	940	189	0.048053731	0.452655273	1.65017	0.001070469	0.032725609	↓
L-Malic acid	931	73	1.420178552	3.606278194	1.81226	0.0003746	0.022123872	↓
myo-inositol	908	305	0.644520791	0.938527873	1.42037	0.049925453	0.205160645	↓
ribitol	891	217	0.021838285	0.016814284	1.86774	0.002222764	0.043313654	↑
oxoproline	888	156	3.926525355	6.182043293	1.67271	0.025586181	0.170987658	↓
gluconic acid 1	882	73	0.104425628	0.758667886	1.94603	0.046718527	0.202243948	↓
Glucose-1-phosphate	879	217	0.10350785	0.199411461	1.44722	0.026246916	0.172471092	↓
mannose 1	868	160	0.070703305	0.528706208	1.11036	0.016089506	0.142292192	↓
inosine 5'-monophosphate	853	169	0.031438759	0.145805445	1.49111	0.020073717	0.156320768	↓
fructose-6-phosphate	847	315	0.219088157	0.628467785	1.10596	0.018654934	0.151726499	↓
asparagine 4	824	115	0.011873179	0.031170614	1.98253	0.000720952	0.026636445	↓
citric acid	823	273	0.038713261	0.00784358	1.43791	0.04983366	0.20508128	↑
trans-4-hydroxy-L-proline 2	781	140	0.20108066	0.253564403	1.60727	0.034944576	0.188208925	↓
ribose	769	217	0.053374799	0.12552007	1.6854	0.01756391	0.14790608	↓
1,5-Anhydroglucitol	717	217	0.069081618	0.001116693	1.84208	0.009992748	0.111687401	↑

Peak	Similarity	Mass	MEAN 12 d	MEAN 8 d	VIP	P-VALUE	Q-VALUE	UP/DOWN
acetanilide 3	890	174	0.031038982	0.012658029	1.70764	0.004057131	0.271344843	↑
taurine	852	326	0.690828681	7.60338E-09	2.09551	0.011736368	0.447217737	↑
ribose	769	217	0.013903507	0.053374799	1.4105	0.039155019	0.58828436	↓
lactose 1	755	204	0.009624694	0.004515707	1.80219	0.002980396	0.271344843	↑
xylose 1	729	117	0.023398229	0.00447303	1.07181	0.033546708	0.575298569	↑
1,5-Anhydroglucitol	717	217	0.021858169	0.069081618	1.70569	0.030751114	0.567349788	↓

Variables	Correlation Matrix
Variables	M7823-80	M8414-05	M10880-9	M3130-58	M5109-45	M6199-14	M7451-79	M7668-64	M7709-26
D-Glyceric acid	-0.4464	-0.2714	-0.2464	-0.2571	0.4821	-0.3642	-0.5678	-0.3607	0.2964
asparagine	-0.5571	-0.3821	-0.2071	-0.3321	0.5857	-0.3714	-0.6214	-0.3892	0.3785
Glucose-1-phosphate	-0.0571	-0.9142	-0.7928	-0.8107	0.5178	-0.425	-0.4428	-0.7464	0.8642
inosine 5'-monophosphate	0.0392	-0.8821	-0.7392	-0.8	0.4892	-0.3714	-0.3928	-0.7357	0.8214
ribose	-0.375	-0.3857	-0.3071	-0.4357	0.4535	-0.3214	-0.375	-0.2428	0.5
acetanilide 3	0.2357	-0.675	-0.7357	-0.875	0.675	-0.4464	-0.2964	-0.3535	0.7785
aspartic acid 1	0.0607	0.5857	0.5214	0.4392	-0.2035	0.125	0.1928	0.4285	-0.3964
phenylalanine 1	0.078571429	0.585714286	0.517857143	0.392857143	-0.178571429	0.146428571	0.2142 85714	0.496428571	-0.453571429

Variables	Correlation Matrix
Variables	M8136-55	M9074-86	M11722-8	M11870-3	M12826-1	M34203-3	M38968-8
D-Glyceric acid	-0.5392	-0.5428	0.7607	0.6	0.5142	0.3214	-0.5107
asparagine	-0.5678	-0.5035	0.8214	0.8464	0.65	0.475	-0.7071
Glucose-1-phosphate	-0.5	-0.5928	0.4821	0.5571	0.6821	0.625	-0.3571
inosine 5'-monophosphate	-0.3428	-0.55	0.3678	0.4321	0.6214	0.5428	-0.1821
ribose	-0.5714	-0.4964	0.6642	0.6142	0.5	0.5321	-0.7071
acetanilide 3	-0.5571	-0.4571	0.3	0.1928	0.4892	0.2464	-0.3142
aspartic acid 1	0.1785	0.1642	0.1285	-0.2	-0.2678	-0.44641	-0.2642
phenylalanine 1	0.1571	0.2214	0.0785	-0.2357	-0.2464	-0.5035	-0.175

Variables	p-value
Variables	M7823-80	M8414-05	M10880-9	M3130-58	M5109-45	M6199-14	M7451-79	M7668-64	M7709-26
D-Glyceric acid	0.0972	0.3268	0.3748	0.3537	0.0711	0.1823	0.0298	0.1869	0.2826
asparagine	0.0335	0.1606	0.4577	0.2263	0.0242	0.1734	0.0155	0.1524	0.1648
Glucose-1-phosphate	0.8424	0	0.0006	0.0003	0.0506	0.1159	0.1001	0.0020	0
inosine 5'-monophosphate	0.8929	0	0.0023	0.0005	0.0665	0.1734	0.1484	0.0025	0.00025
ribose	0.1691	0.1565	0.2649	0.1062	0.0915	0.2424	0.1691	0.3819	0.0602
acetanilide 3	0.3965	0.0072	0.0025	0	0.0072	0.0972	0.2826	0.1963	0.0009
aspartic acid 1	0.8324	0.0242	0.0488	0.1031	0.4657	0.6575	0.4900	0.1126	0.1445
phenylalanine 1	0.7826	0.0242	0.0506	0.1484	0.5235	0.6023	0.4420	0.0622	0.0915

Variables	p-value
Variables	M8136-55	M9074-86	M11722-8	M11870-3	M12826-1	M34203-3	M38968-8
D-Glyceric acid	0.0406	0.0391	0.0015	0.0204	0.0524	0.2424	0.0542
asparagine	0.0298	0.0581	0.0002	6.0047	0.0105	0.0758	0.0043
Glucose-1-phosphate	0.0602	0.0222	0.0711	0.0335	0.0065	0.0148	0.1916
inosine 5'-monophosphate	0.2109	0.0362	0.1778	0.1094	0.0155	0.0391	0.5150
ribose	0.0286	0.0622	0.0085	0.0170	0.0602	0.0437	0.0043
acetanilide 3	0.0335	0.0887	0.2766	0.4900	0.0665	0.3748	0.2535
aspartic acid 1	0.5235	0.5579	0.6482	0.4737	0.3334	0.0972	0.3401
phenylalanine 1	0.5755	0.4265	0.7826	0.3965	0.3748	0.0581	0.5320

Differential metabolites	Metabolic pathway
fumaric acid	Citrate cycle
	Arginine biosynthesis
	Alanine aspartate and glutamate methabolism
	Tyrosine methabolism
	Phenylalanine methabolism
	Butanoate methabolism
	Nicotinate and Nicotinamide methabolism
D-Glyceric acid	Pentose phosphate pathway
	Glycine serine and threonine methabolism
	Glycerolipid methobolism
	Glyoxylate and dicarboxylate methabolism
	Methane methabolism
phenylalanine 1	Phenylalanine methabolism
methionine 1	Cysteine and methionine methabolism
aspartic acid 1	Alanine aspartate and glutamate methabolism
	Histidine methabolism
	Cyanoamino acid methabolism
asparagine 4	Alanine aspartate and glutamate methabolism
asparagine 4	Cyanoamino acid methabolism
Glucose-1-phosphate	Pentose and glucuronate interconversions
Glucose-1-phosphate	Embden meyerhof parnas
sn-(glycerol 3-phosphate)	Embden meyerhof parnas
	Glycerolipid methabolism
	Amino sugar and nucleotide sugar methabolism
Inosine-5'-monophosphate	Purine methabolism
lysine	Lysine biosynthesis
1,3-diaminopropane	Glycine serine and threonine methabolism
1,3-diaminopropane	β-Alanine methabolism
terephthalic acid	Polycyclic aromatic hydrocarbon degration
5'-methylthioadenosine 1	Cysteine and methionine methabolism
Isomaltose 1	Starch and sucrose methabolism
2-Hydroxyvaleric acid	/
acetanilide 3	/
L-Malic acid	Glyoxylate and dicarboxylate methabolism
	Methane methabolism
	Citrate cycle
myo-inositol	Galactose methabolism
ribitol	Pentose and glucuronate interconversions
oxoproline	Glutathione methabolism
gluconic acid 1	Pentose phosphate pathway
mannose 1	Galactose methabolism
mannose 1	Amino sugar and nucleotide sugar methabolism
fructose-6-phosphate	Methane methabolism
	Embden meyerhof parnas
	Amino sugar and nucleotide sugar methabolism
citric acid	Citrate cycle
citric acid	Glyoxylate and dicarboxylate methabolism
ribose	Pentose phosphate pathway
trans-4-hydroxy-L-proline 2	/
1,5-Anhydroglucitol	/
taurine	/
xylose 1	Galactose methabolism
lactose 1	Pentose and glucuronate interconversions

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[1] *Corresponding author: ruimingluo.nx@163.com