Mulberry leaf polysaccharide extracted by response surface methodolog suppresses the proliferation, invasion and migration of MCF-7 breast cancer cells

Abstract

Breast cancer with highly heterogeneous characteristics is the main cause of cancer deaths in women all over the world. Chemotherapy-assisted diet therapy has become an effective way to improve the quality of life of cancer patients. Currently numerous dietary components have been found to inhibit the molecular events and signalling pathways associated with various stages of breast cancer development, but the dietary components extraction and its regulation mechanism were long-term and complex. In this study, low-temperature ultrasonic-assisted method was used to extract MLP, and through employing the Design-Expert software, the optimal conditions for MLP extraction were ultrasonic power 179.34 W, ultrasonic time 13.92 min, and ratio (v/w, mL/g) of water to raw material 23.55. Subsequent experiments evaluated the antioxidant activity of mulberry leaf polysaccharide and found that it has obvious ability to scavenge DPPH free radicals. Interestingly, the extracted MLP could inhibit the cell viability, migration and invasion of breast cancer cells in vitro. Therefore, we suggested MLP could been used as an antioxidant supplement that contains these micronutrients for cancer patients or used to develop anti-oxidant functional health food for breast cancer patients.

Keywords:
mulberry leaf polysaccharide; response surface methodolog; antioxidant; breast cancer; cell viability