Microbial diversity analysis of vineyard son the eastern foothills of the Helan Mountain region using high-throughput sequencing

Abstract

In this study, Illumina MiSeq was used to investigate the microbial diversity ofwine grapes and soil from three vineyards indifferent localities on the eastern foothills of Helan Mountain in Ningxia, China. The results of 26 samples showed thatthere were 4 fungal phyla with 1107 operational taxonomic units (OTUs) and 11 bacterial phyla with 4630 OTUs. At the genus level, the dominant fungi in the grapes included Aspergillus, Alternaria, Exobasidum, Aureobasidium, and Cladosporium, while the dominant fungi in the soil included Gibberella, Gliomastix, Coprinellus, Fusarium, and Chaetomium, Chryseobacterium, Mesorhizobium, Pseudomonas, Microbacterium and Acinetobacter were the dominant bacteria in the grapes, while Arthrobacter, Pontibacter, Massilia, Skermanella, and Candiatus-Nitrosocosmicus were the dominant bacteria in the soil. Principal component analysis showed that the microbial diversity differed in the three vineyards from different geographical locations. This study provides a theoretical basis for the utilization and development of the characteristic microorganisms in vineyards.

Keywords:
the eastern foothills of Helan Mountain wine region; high-throughput sequencing; microbial diversity; wine grapes; soil