Solid phase microextraction (SPME) analysis of volatile compounds produced by in vitro shoots of Lantana camara L. under the influence of auxins and cytokinins

Abstract

The effects of the auxin indole-3-acetic acid at 0.44 µmol L-1 and the cytokinins thidiazuron at 0.44 µmol L-1 and benzyladenine at 0.44 and 4.4 µmol L-1 on growth, development and volatile compounds production of Lantana camara L. in vitro plants were investigated. The volatiles were extracted by solid phase microextraction (SPME) and the quantification was performed using GC-MS. A higher content of myrcene, alpha-phellandrene, alpha-copaene, trans-caryophyllene and beta-gurjunene, and the lower levels of alpha-pinene and beta-pinene were observed in in vitro plants cultivated on different supplementation media than in donor plants.

Lantana camara L.; volatile compounds; SPME; growth regulators; tissue culture