Standardization of the accelerated aging test for evaluation of wheat seed vigor

Favoretto, Marli de Moraes Gomes; Krzyzanowski, Francisco Carlos; Emrich, Patricia Piva; Zucareli, Claudemir

doi:10.1590/2317-1545v46279725

Abstract:

The accelerated aging test is widely used to evaluate seed vigor. However, for the wheat crop, discrepancies are found in the literature regarding temperature and period of exposure to stress. This study aimed to evaluate the efficiency of the accelerated aging test in different combinations of time and temperature in seed vigor evaluation in wheat lots and cultivars. The following cultivars were used: Tbio Sintania, Tbio Sossego, Tbio Sinuelo, LG Oro, Tbio Toruk, and CD 150 (Group 1), represented by one lot each, and five lots of the cultivar CD 150 (Group 2). Water content, germination, first count of germination, and seedling emergence in the field were evaluated for characterization of initial physiological potential. Then the accelerated aging test was applied to the lots under the following aging conditions: 41 °C / 48 and 72 h, 43 °C / 40 and 48 h, and 45 °C / 24 and 40 h in plastic boxes, with 40 mL of distilled water and seeds distributed in a single layer on a screen. After aging, the water content and germination of the seeds were determined. Analysis of variance was performed on the data, and the means were compared by Tukey’s test, at 5% probability. The simple correlation test (r) was performed between the analyzed variables and the seedling emergence in the field test. The accelerated aging test with the combinations of 41 °C / 72 h and 43 °C / 48 h is efficient for evaluating seed vigor of wheat lots and cultivars. The combination of 43 °C / 48 h provides results more quickly, saving 24 h in relation to the methodology recommended in the literature (41 °C / 72 h).

Index terms:
abiotic stresses; deterioration; physiological potential; Triticum aestivum L

[1] Corresponding author
patricia.piva.emrich@uel.br.

Cultivar	WCI	41 °C		43 °C			45 °C
Cultivar	WCI	48 h	72 h	40 h	48 h	24 h	40 h
1-Tbio Sintonia	11.0	27.4	28.7	27.0	28.7	24.5	28.2
2-Tbio Sossego	10.6	27.9	29.3	27.5	28.9	25.6	27.7
3-Tbio Sinuelo	10.0	27.2	29.1	26.1	27.2	24.3	26.7
4-LG Oro	11.4	28.6	28.8	28.1	29.3	25.9	28.6
5-Tbio Toruk	11.2	28.4	29.3	27.2	28.9	25.0	26.5
6-CD 150	10.2	26.6	28.1	26.9	27.3	24.3	28.7

Lot	WCI	41 °C		43 °C		45 °C
Lot	WCI	48 h	72 h	40 h	48 h	24 h	40 h
1	10.2	26.5	28.1	26.9	27.3	24.3	28.3
2	10.1	26.7	28.2	25.7	27.2	23.1	26.4
3	11.1	26.8	28.3	26.3	29.2	24.0	27.7
4	10.4	26.5	28.5	26.3	28.3	25.0	26.7
5	10.9	26.8	27.4	26.6	27.9	24.0	27.8

Source of Variation		Mean Squares
Source of Variation	DF	G	FC
Cultivar	5	14.34	239.58
Error	18	1.90	4.76
F-test ^(Cal)	-	7.54^ Significant at 1% probability by the F-test; ns not significant.	50.29^ Significant at 1% probability by the F-test; ns not significant.
		SEF	F-test ^(Cal)
Cultivar	5	141.07	26.12^ Significant at 1% probability by the F-test; ns not significant.
Block	3	3.83	0.71^ns
Error	15	5.40	-

Cultivar	G	FC	SEF
Cultivar	----------------------------------------%-------------------------------------
1-Tbio Sintonia	88 b^* * Mean values followed by different letters in the column differ from each other by Tukey’s test (p ≤ 0.05).	66 c	76 d
2-Tbio Sossego	92 a	85 a	84 bc
3-Tbio Sinuelo	92 a	86 a	87 ab
4-LG Oro	91 a	81 b	79 cd
5-Tbio Toruk	93 a	86 a	90 a
6-CD 150	93 a	86 a	91 a
CV (%)	1.51	2.68	2.75

Source of Variation	Mean Squares
Source of Variation	DF	G	FC
Lot	4	57.43	1754.63
Error	15	5.68	7.10
F-test ^(Cal)	-	10.10^ Significant at 1% probability by the F-test; ns not significant.	247.13^ Significant at 1% probability by the F-test; ns not significant.
		SEF	F-test ^(Cal)
Lot	4	158.55	19.45^ Significant at 1% probability by the F-test; ns not significant.
Block	3	3.73	0.46 ^ns
Error	12	8.15	-

Brasil

Brasil

Standardization of the accelerated aging test for evaluation of wheat seed vigor

Abstract:

Lot	G	FC	SEF
Lot	-----------------------------------------%----------------------------------
1	93 a^* * Mean values followed by different letters in the column differ from each other by Tukey’s test (p ≤ 0.05).	86 a	91 a
2	95 a	86 a	92 a
3	86 b	51 b	79 b
4	88 b	43 c	79 b
5	94 a	82 a	87 a
CV (%)	2.62	3.83	3.33

Variables	Seedling emergence in the field
Variables	Group 1	Group 2
Germination	0.91^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.93^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.
First Count of Germination	0.83^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.97^ significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.
Accelerated Aging 41 °C / 48 h	0.84^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.86 ^ns
Accelerated Aging 41 °C / 72 h	0.95^ significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.95^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.
Accelerated Aging 43 °C / 40 h	0.84^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.72 ^ns
Accelerated Aging 43 °C / 48 h	0.97^ significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.93^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.
Accelerated Aging 45 °C / 24 h	0.91^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.78 ^ns
Accelerated Aging 45 °C / 40 h	0.94^* ** significant at 1% probability by the t-test; * significant at 5% probability by the t-test; ns not significant.	0.83 ^ns