The isolation of tosigenic Corynebacterum diphtheriae from sperm is reported. The organism was identified through the investigation of fluorescence under the UV light, the presence of pirazinecarboxilamidase enzyme (Pyz), in vitro and in vivo and virulence methods (single radial immunodiffusion, cell culture, guiena pig intradermic test). The strain was initially cosnsidered montoxinogenic by single radial immunodiffusion, but its virulence was observed afterwards, when we applied the tests already mentioned. The strain could be considered a "Diphtheroid" without adequate specification.
Corynebacterium diphtheriae; diphtheroid; sperm; mass screening; cell culture