Composition of media for <i>in vitro</i> slow growth storage (sgs) of Aglaonema

Nugrahani, Pangesti; Purnobasuki, Hery; Sitawati, Sitawati

doi:10.1590/2447-536X.v30.e242696

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Article • Ornam. Hortic. 30 • Jan-Dec 2024 • https://doi.org/10.1590/2447-536X.v30.e242696 copy

Composition of media for in vitro slow growth storage (sgs) of Aglaonema

Composição de meios de crescimento lento para armazenamento in vitro (sgs) de Aglaonema

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Aglaonema is one of the ornamental plant commodities often affected by falling prices in the ornamental plant market. This phenomenon requires a strategy for storing seeds of rare and exotic cultivars for the short and medium term. In vitro storage is one way to anticipate it. This study aims to obtain the suitable composition of in vitro growing media for storing Aglaonema plants by slow growth storage (SGS). This study used a completely randomized design with one factor, consisting of seven treatments, i.e., media 1/4 Murashige and Skoog (MS), 1/2 MS, and full MS, with the addition of 1.0 mg L^-1 and 2.0 mg L^-1 Benzyl aminopurine (BAP), and the addition of Indoleacetic Acid (IAA) 1.0 mg L^-1 and 2.0 mg L^-1. The results showed that the composition of the planting medium on ¼ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1 was the best for slow-growth storage of Aglaonema in vitro.

Keywords:
Aglaonema commutatum ‘Lady Valentine’; benzyl aminopurine; in vitro storage; indoleacetic acid; Murashige and Skoog

No.	Treatment code	Media composition
1	P0	MS without PGR
2	P1	MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1
3	P2	MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1
4	P3	½ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1
5	P4	¼ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1
6	P5	½ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1
7	P6	¼ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1

Media composition	average time of emergence
Media composition	shoots	roots	leaves
P0: MS without PGR	12.56 a	21.89 c	1.89
P1: MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	13.56 a	4.94 a	-
P2: MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	16.44 a	13.50 bc	-
P3: ½ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	13.67 a	17.83 c	-
P4: ¼ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	24.89 b	13.33 b	-
P5: ½ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	12.78 a	18.33 c	-
P6: ¼ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	13.11 a	14.67 bc	-
Tukey’s 5%	3.91	4.39

Media Composition	Plantlet length (cm)	Number of Shoots	Number of Roots
P0: MS without PGR	4.86 b	1.61 a	1.89 b
P1: MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	2.42 a	1.44 a	0.78 ab
P2: MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	2.83 a	2.33 ab	0.56 ab
P3: ½ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	3.56 ab	1.78 a	1.89 b
P4: ¼ MS + BAP 1.0 mg L^-1 + IAA 1.0 mg L^-1	2.17 a	1.89 ab	0.11 a
P5: ½ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	3.44 ab	3.00 b	1.11 ab
P6: ¼ MS + BAP 2.0 mg L^-1 + IAA 2.0 mg L^-1	3.94 ab	2.33 ab	0.89 ab
Tukey’s 5%	1.72	1.15	1.37

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[1] * Correspondent author: email pangesti_n@upnjatim.ac.id