Conservação in vitro de físalis por meio do cultivo de segmentos nodais em crescimento lento

O objetivo deste trabalho foi avaliar os efeitos da temperatura e de agentes osmóticos na conservação in vitro de físalis (Physalis peruviana). Foram testadas as temperaturas de 18 e 25°C, bem como os agentes osmóticos sacarose, manitol e sorbitol. A conservação de físalis in vitro, em curto prazo, pode ser conseguida a 18°C, em meio com adição de 30 g L^-1 de sacarose.

Termos para indexação:
Physalis peruviana; conservação ex situ; cultura de tecidos.

Autoria

Renata Alves Lara Silva Rezende

Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3.037, CEP 37200-000 Lavras, MG, Brazil. E-mail: renata_vga@yahoo.com.br, filipealmendagna@yahoo.com.br, joyce.soares@dag.ufla.br, mpasqual@dag.ufla.br, franscinelyagronomia@yahoo.com.brUniversidade Federal de LavrasBrazilLavras, MG, BrazilUniversidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3.037, CEP 37200-000 Lavras, MG, Brazil. E-mail: renata_vga@yahoo.com.br, filipealmendagna@yahoo.com.br, joyce.soares@dag.ufla.br, mpasqual@dag.ufla.br, franscinelyagronomia@yahoo.com.br

Filipe Almendagna Rodrigues

Ramiro Machado Rezende

Universidade Vale do Rio Verde, Departamento de Agronomia, Avenida Castelo Branco, no 82, Chácara das Rosas, CEP 37410-000 Três Corações, MG, Brazil. E-mail: ramiromr@globo.comUniversidade Vale do Rio VerdeBrazilTrês Corações, MG, BrazilUniversidade Vale do Rio Verde, Departamento de Agronomia, Avenida Castelo Branco, no 82, Chácara das Rosas, CEP 37410-000 Três Corações, MG, Brazil. E-mail: ramiromr@globo.com

Joyce Dória Rodrigues Soares

Moacir Pasqual

Franscinely Aparecida de Assis

SCIMAGO INSTITUTIONS RANKINGS

Figuras | Tabelas

Figuras (1)
Tabelas (2)

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Figure 1
Number of leaves, leaf abscission, and shoot length of Cape gooseberry (Physalis peruviana), at the growth recovery phase, after in vitro conservation at different temperatures and osmotic agents. Means with different letters indicate significant differences between treatments, by Tukey’s test, at 5% probability. Lowercase letters in figures A, B, and C. Figure D: uppercase letters indicate differences between osmotic agents, at each temperature, and lowercase letters indicate differences between temperatures.

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Table 1.
Viability (scores), number of leaves, number of buds and leaf abscission of Cape gooseberry (Physalis peruviana), at different temperatures and osmotic regulator treatments, at 30, 60, and 90 days⁽¹⁾.

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Table 2.
Shoot length, fresh and dry weight of shoots, and fresh and dry weight of roots of Cape gooseberry (Physalis peruviana), as a function of temperature and osmotic regulators, at 90 days⁽¹⁾.

Figure 1 Number of leaves, leaf abscission, and shoot length of Cape gooseberry (Physalis peruviana), at the growth recovery phase, after in vitro conservation at different temperatures and osmotic agents. Means with different letters indicate significant differences between treatments, by Tukey’s test, at 5% probability. Lowercase letters in figures A, B, and C. Figure D: uppercase letters indicate differences between osmotic agents, at each temperature, and lowercase letters indicate differences between temperatures.

Table 1. Viability (scores), number of leaves, number of buds and leaf abscission of Cape gooseberry (Physalis peruviana), at different temperatures and osmotic regulator treatments, at 30, 60, and 90 days⁽¹⁾.

Treatment (days)	Culture media with osmotic regulators
Treatment (days)	Sucrose	Sucrose + mannitol	Sucrose + sorbitol
	Viability (scores)
	------------------------------------------------------------18°C------------------------------------------------------------
30	2.40aAα	3.07aAα	3.33aAα
60	3.97bAα	5.43bBα	5.07bBα
90	5.53cAα	6.87cBα	7.93cBα
	25°C
30	6.03aCβ	2.63aAα	4.33aBβ
60	7.77bBβ	5.07bAα	5.30bAα
90	12.23cBβ	7.03cAα	7.43cAα
CV (%)	21.6
	Number of leaves
	------------------------------------------------------------18°C------------------------------------------------------------
30	2.40aAα	3.07aAα	3.33aAα
60	3.97bAα	5.43bBα	5.07bBα
90	5.53cAα	6.87cBα	7.93cBα
	25°C
30	6.03aCβ	2.63aAα	4.33aBβ
60	7.77bBβ	5.07bAα	5.30bAα
90	12.23cBβ	7.03cAα	7.43cAα
CV (%)	21.6
	Number of buds
	------------------------------------------------------------18°C------------------------------------------------------------
30	2.23aAα	2.37aAα	2.90aAα
60	3.93aAα	5.20bBα	4.43bABα
90	5.40bAα	6.40cABα	7.30cBα
	------------------------------------------------------------25°C------------------------------------------------------------
30	4.73aCβ	2.43aAα	3.63aBα
60	7.00bBβ	5.40bAα	6.10bABβ
90	11.37cBβ	6.60cBAα	7.00cAα
CV (%)	24.6
	Leaf abscission (%)
	------------------------------------------------------------18°C------------------------------------------------------------
30	0.00aAα	0.00aAα	0.00aAα
60	1.39aAα	0.48aAα	11.53bBα
90	6.30aAα	2.00aAα	20.22cBα
	------------------------------------------------------------25°C------------------------------------------------------------
30	17.18aBβ	0.00aAα	16.06aBβ
60	45.00bCβ	8.10bAα	22.90bBβ
90	51.23bCβ	8.80bAα	26.40bBα
CV (%)	82.1

⁽¹⁾
Means followed by equal lower case letters, in the columns of the same temperature, with upper case letters in rows, and Greek letters between temperatures in the columns, in the same evaluations, showed no significant differences, by the Tukey’s test, at 5% probability.

Table 2. Shoot length, fresh and dry weight of shoots, and fresh and dry weight of roots of Cape gooseberry (Physalis peruviana), as a function of temperature and osmotic regulators, at 90 days⁽¹⁾.

Temperature (ºC)	Culture media with osmotic regulators
Temperature (ºC)	Sucrose	Sucrose + mannitol	Sucrose + sorbitol
	Shoot length (cm)
18	12.27aB	5.52aA	18.47bC
25	15.68bB	7.43aA	16.25aB
CV (%)	18.9
	Fresh weight of shoots (g)
18	0.1974aA	0.1368aA	0.3585aB
25	1.1983bC	0.1725aA	0.4142aB
CV (%)	30.9
	Dry weight of shoots (g)
18	0.0140aA	0.0135aA	0.0238aB
25	0.0729bB	0.0153aA	0.0235aA
CV (%)	35.0
	Fresh weight of roots (g)
18	0.0186aA	0.0054aA	0.0617aB
25	0.2433bB	0.0114aA	0.0417aA
CV (%)	68.3
	Dry weight of roots (g)
18	0.0006aA	0.0014aA	0.0042aA
25	0.0231bB	0.0020aA	0.0035aA
CV (%)	71.1

⁽¹⁾
Means followed by equal lower case letters in the columns, and upper case letters in the rows, showed no significant differences, by the Tukey’s test, at 5% probability.

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Conservação in vitro de físalis por meio do cultivo de segmentos nodais em crescimento lento

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[TFN1] ⁽¹⁾
Means followed by equal lower case letters, in the columns of the same temperature, with upper case letters in rows, and Greek letters between temperatures in the columns, in the same evaluations, showed no significant differences, by the Tukey’s test, at 5% probability.