Abstract:
The objective of this work was to assess the androgenic response, via microspore culture, of wheat genotypes with different levels of resistance to Gibberella zeae. The number of androgenic embryos per spike, and of green and albino plants was counted for the BRS 179 (moderately resistant), Frontana and Sumai 3 (resistant), and BRS 194, Embrapa 27, and Fielder (susceptible) genotypes. The degree of interference by the Fielder, Pavon 76, and Sumai 3 ovary-donor genotypes, used for co-culture with the microspore cells, was also assessed regarding androgenic response. Induction efficiency ranged from 0.33 embryo per spike for Embrapa 27 to 109.8 embryos for Frontana. Sumai 3 presented the second best response, and Embrapa 27 behaved as highly recalcitrant. The co-culture of ovaries from the Fielder genotype stands out from the others and positively interferes in the embryo induction rate. No significant differences were observed for the regeneration frequency of green plants; however, for albino plants, BRS 194 produced the highest rate. Frontana and Sumai 3 present the highest androgenic response rates and can be used in breeding programs for the rapid development of cultivars resistant to Gibberella zeae.
Index terms:
Gibberella zeae; Triticum aestivum; doubled haploids; microspore
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