Suppression of female flowers and pollen fertility of male flowers of banana plants

Dzoyem, Camille Ulrich Dzokouo; Kapuku, Lucien Ibobondji; Ndiang, Zenabou; Tchabong, Gaetan Romaric Ngapmeu; Youmbi, Emmanuel; Bakry, Frédéric

doi:10.1590/S1678-3921.pab2023.v58.03376

Acessibilidade / Reportar erro

Brasil

Pesquisa Agropecuária Brasileira

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Genetics / Original Article • Pesq. agropec. bras. 58 • 2023 • https://doi.org/10.1590/S1678-3921.pab2023.v58.03376 copy

Suppression of female flowers and pollen fertility of male flowers of banana plants

Supressão de flores femininas e fertilidade polínica de flores masculinas de bananeira

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The objective of this work was to evaluate the effect of the suppression of female flowers on the pollen fertility of male flowers of banana plants. Two modes of female flower suppression, one partial and one complete, were applied shortly after flowering to diploid, triploid, and tetraploid banana genotypes. The effect of flower suppression on the number of male flowers and the length of their anthers was evaluated, as well as pollen viability and the size and number of pollen grains per anther. Pollination tests were performed to evaluate the number of seed obtained from the progeny of some genotypes. The total suppression of female flowers significantly increases pollen fertility, estimated by an increase in the number of pollen grains per anther (up to 80% per anther). Total female flower suppression also improves significantly seed production in the crosses (from 35 to 160% per pollinated bunch), which suggests a positive influence of the sap flowing through the bunch on the efficiency of banana pollen.

Index terms
Musa; genotype; hybridization; pollen inefficiency; sap flow; seed

Accession	Ploidy and genome	Group	Origin	Accession number⁽¹⁾	Study site	For more details see
Calcutta 4	2x (AA)	Wild	Indonesia	CMR00444	Njombe	Ongagna et al. (2020)ONGAGNA, A.; DZOKOUO DZOYEM, C.U.; YOUMBI, E.; BAKRY, F. Positive effect of in vitro embryo rescue on breaking the dormancy of wild banana seeds compared to direct sowing in the greenhouse. Fruits, v.75, p.194-203, 2020. DOI: https://doi.org/10.17660/th2020/75.5.2. https://doi.org/10.17660/th2020/75.5.2...
Khae Phrae	2x (AA)	Wild	Thailand	KHA	Njombe	Martin et al. (2020)MARTIN, G.; CARDI, C.; SARAH, G.; RICCI, S.; JENNY, C.; FONDI, E.; PERRIER, X.; GLASZMANN, J.C.; D’HONT, A.; YAHIAOUI, N. Genome ancestry mosaics reveal multiple and cryptic contributors to cultivated banana. The Plant Journal, v.102, p.1008-1025, 2020. DOI: https://doi.org/10.1111/tpj.14683. https://doi.org/10.1111/tpj.14683...
Balbisiana Cameroun	2x (BB)	Wild	Unknown	BCMR	Njombe	Ongagna et al. (2020)ONGAGNA, A.; DZOKOUO DZOYEM, C.U.; YOUMBI, E.; BAKRY, F. Positive effect of in vitro embryo rescue on breaking the dormancy of wild banana seeds compared to direct sowing in the greenhouse. Fruits, v.75, p.194-203, 2020. DOI: https://doi.org/10.17660/th2020/75.5.2. https://doi.org/10.17660/th2020/75.5.2...
Pisang Lilin	2x (AA)	Cultivar	Unknown	LILI	Njombe	Martin et al. (2020)MARTIN, G.; CARDI, C.; SARAH, G.; RICCI, S.; JENNY, C.; FONDI, E.; PERRIER, X.; GLASZMANN, J.C.; D’HONT, A.; YAHIAOUI, N. Genome ancestry mosaics reveal multiple and cryptic contributors to cultivated banana. The Plant Journal, v.102, p.1008-1025, 2020. DOI: https://doi.org/10.1111/tpj.14683. https://doi.org/10.1111/tpj.14683...
Tomolo	2x (AA)	Cultivar	Papua New Guinea	TOMO	Njombe	Bakry et al. (2007)BAKRY, F.; REBERDIERE, N.P. de la; PICHOT, S.; JENNY, C. In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of monoand interspecific diploid banana clones. Fruits, v.62, p.3-12, 2007. DOI: https://doi.org/10.1051/fruits:2006043. https://doi.org/10.1051/fruits:2006043...
Mshale	2x (AA)	Cultivar	Highland of East Africa	CMR04165	Mantem	Waniale et al. (2021)WANIALE, A.; SWENNEN, R.; MUKASA, S.B.; TUGUME, A.K.; KUBIRIBA, J.; TUSHEMEREIRWE, W.K.; BATTE, M.; BROWN, A.; TUMUHIMBISE, R. Seed set patterns in East African highland cooking bananas show asymmetric distribution in bunches and fruits. Agronomy, v.11, art.763, 2021. DOI: https://doi.org/10.3390/agronomy11040763. https://doi.org/10.3390/agronomy11040763...
Mshare Mrerembo	2x (AA)	Cultivar	Highland of East Africa	CMR04167	Mantem	Perrier et al. (2019)PERRIER, X.; JENNY, C.; BAKRY, F.; KARAMURA, D.; KITAVI, M.; DUBOIS, C.; HERVOUET, C.; PHILIPPSON, G.; DE LANGHE, E. East African diploid and triploid bananas: a genetic complex transported from South-East Asia. Annals of Botany, v.123, p.19-36, 2019. DOI: https://doi.org/10.1093/aob/mcy156. https://doi.org/10.1093/aob/mcy156...
Pisang Madu	2x (AA)	Cultivar	Malaysia	MADU	Mantem	Martin et al. (2020)MARTIN, G.; CARDI, C.; SARAH, G.; RICCI, S.; JENNY, C.; FONDI, E.; PERRIER, X.; GLASZMANN, J.C.; D’HONT, A.; YAHIAOUI, N. Genome ancestry mosaics reveal multiple and cryptic contributors to cultivated banana. The Plant Journal, v.102, p.1008-1025, 2020. DOI: https://doi.org/10.1111/tpj.14683. https://doi.org/10.1111/tpj.14683...
Yangambi Km5	3x (AAA)	Cultivar	Congo (Kinshasa)	YGBI	Mantem	Fogain & Gowen (1998)FOGAIN, R.; GOWEN, S.R. “Yangambi km5” (Musa AAA, Ibota subgroup): a possible source of resistance to Radopholus similis and Pratylenchus goodeyi. Fundamental and Applied Nematology, v.21, p.75-80, 1998.
CARBAP 832	4x (AAAB)	Hybrid	Cameroon	CMR00446	Njombe	Dépigny et al. (2018)DÉPIGNY, S.; NOUPADJA, P.; TOMEKPE, K.; BONNEVIOT, P.; LESCOT, T.; BAKRY, F. 'CARBAP K74': a triploid plantain-like hybrid designed to promote sustainable plantain-based cropping systems. Acta Horticulturae, v.1196, p.63-70, 2018. DOI: https://doi.org/10.17660/ActaHortic.2018.1196.7. https://doi.org/10.17660/ActaHortic.2018...
FHIA 21	4x (AAAB)	Cultivar	Honduras	ITC1306	Mantem	Tenkouano et al. (2019)TENKOUANO, A.; LAMIEN, N.; AGOGBUA, J.; AMAH, D.; SWENNEN, R.; TRAORÉ, S.; THIEMELE, D.; ABY, N.; KOBENAN, K.; GNONHOURI, G.; YAO, N.; ASTIN, G.; SAWADOGO-KABORE, S.; TARPAGA, V.; ISSA, W.; LOKOSSOU, B.; ADJANOHOUN, A.; AMADJI, G.L.; ADANGNITODE, S.; IGUE, K.A.D.; ORTIZ, R. Promising high-yielding tetraploid plantain-bred hybrids in West Africa. International Journal of Agronomy, v.2019, art.3873198, 2019. DOI: https://doi.org/10.1155/2019/3873198. https://doi.org/10.1155/2019/3873198...
CRBP 39	4x (AAAB)	Hybrid	Cameroon	CMR00451	Mantem	Noumbissié et al. (2016)NOUMBISSIÉ, G.B.; CHABANNES, M.; BAKRY, F.; RICCI, S.; CARDI, C.; NJEMBELE, J.-C.; YOHOUME, D.; TOMEKPE, K.; ISKRA-CARUANA, M.-L.; D’HONT, A.; BAURENS, F.-C. Chromosome segregation in an allotetraploid banana hybrid (AAAB) suggests a translocation between the A and B genomes and results in eBSV-free offsprings. Molecular Breeding, v.36, art.38, 2016. DOI: https://doi.org/10.1007/s11032-016-0459-x. https://doi.org/10.1007/s11032-016-0459-...

Accession group	Genotype	Male flowers count	Anther length (mm)	Pollen size (µm)	Pollen number/anther	Pollen viability rate (%)	Potential pollen fertility
Wild diploid	Balbisiana Cameroun	26±2a	18.5±1.5f	94.79±7.09i	9,127±1,493a	98.86±1.54a	9,012±1,384a
	Calcutta 4	18±3b	20.9±2.3d	98.62±9.52h	4,629±706b	94.01±5.23a	4,347±776c
	Khae Phrae	8±2j	14.8±1.7g	104.02±6.98g	4,890±1259b	97.02±3.61a	4,745±1,127bc
Cultivated diploid	Mshale	13±2gh	20.48±2.29e	134.40±16.43c	882±188e	71.21±10.79c	629±123fg
	Mshare Mrerembo	14±1fg	24.98±1.78c	127.11±16.67e	833±211e	72.08±15.29c	602±153fg
	Pisang Lilin	16±2d	15.17±1.67g	131.44±18.33d	1573±39d	95.05±0.75a	1,495±34e
	Pisang Madu	15±4e	18.30±1.79f	110.65±12.41f	2,872±788c	78.48±8.16b	2,277±661d
	Tomolo	12±1i	-	100.37±13.17h	377±19e	79.52±2.21b	300±18g
Triploid	Yangambi km5	18±2c	27.10±2.34b	148.35±20.60a	1,516±404d	48.88±8.25e	727±182f
Tetraploid	CRBP 39	12±1hi	24.77±2.17c	140.11±19.94b	3,911±1,055b	56.85±12.09d	2,174±619d
Tetraploid	FHIA 21	14±2f	28.97±2.56a	134.02±16.73c	8,238±1,869a	69.44±10.08c	5,624±1,100b

Accession group and name	Study site	[64-74]	[74-84]	[84-94]	[94-104]	[104-114]	[114-124]	[124-134]	[134-144]	[144-154]	[154-164]	[164-174]	[174-184]	[184-194]	[194-204]	[204-214]	[214-224]	[224-234]	[234-244]	[244-254]	[254-264]	[264-274]	Total count
Seeded diploid
Calcutta 4	Njombe	3	85	220	413	314	43	2	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	1,080
Khae Phrae	Njombe	˗	4	19	148	165	23	0	1	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	360
Balbisiana Cameroun	Njombe	˗	23	113	190	34	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	360
Cultivated diploid
Tomolo	Njombe	3	48	270	333	152	101	30	15	5	2	1	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	960
Pisang Madu	Mamtem	8	49	722	2,272	2,224	2,503	1,083	263	103	10	3	˗	˗	˗	˗	˗	˗	˗	˗	˗	˗	9,240
Pisang Lilin	Njombe	˗	3	17	85	165	472	414	465	178	145	45	30	10	8	2	0	0	0	0	1	-	2,040
Mshale	Mamtem	˗	3	18	112	236	1,018	1,441	912	1,223	289	201	33	23	8	2	1	˗	˗	˗	˗	˗	5,520
Mshare Mererembo	Mamtem	1	14	14	147	158	471	554	183	280	60	25	6	4	1	0	0	1	0	0	1	˗	1,920
Cultivated triploid
Yangambi km5	Mamtem	˗	1	8	78	149	764	1,526	1,462	2,968	1,049	1,498	376	329	158	33	22	13	3	2	0	1	10,440
Tetraploid hybrid
CRBP 39	Njombe	˗	4	25	75	91	285	467	345	663	240	247	38	32	7	0	0	1	˗	˗	˗	˗	2,520
FHIA 21	Njombe	˗	1	16	149	232	580	748	699	954	206	115	14	5	1	˗	˗	˗	˗	˗	˗	˗	3,720

Accession group	Genotype	Mode of flower suppression	Male flowers count	Anther lenght (mm)	Pollen size (µm)	Pollen number/anther.	Pollen viability rate (%)	Potential pollen fertility
Cultivated diploid	Mshale	T0	13.14	20.48	134.40	881.50	71.21	629.27
		T1	-1.36^* ***** ,	0.2	-5.66^* ***** ,	+298^* ***** ,	+5.23^* ***** ,	+284^* ***** ,
		T2	-1.19^* ***** ,	0.4	-0.85	+270^* ***** ,	+2.27^* ***** ,	+211^* ***** ,

	Mshare Mrerembo	T0	13.95	24.98	127.11	833.11	72.08	601.72
		T1	-2.0^* ***** ,	-2.5^* ***** ,	+2.78^* ***** ,	+131^* ***** ,	-11.97^* ***** ,	-19
		T2	-2.33^* ***** ,	-1.9^* ***** ,	+4.79^* ***** ,	+237^* ***** ,	-4.82^* ***** ,	114

	Pisang Lilin	T0	15.98	15.17	131.50	1573	95.05	1495
		T1	-0.136	+2.56^* ***** ,	-4.55^* ***** ,	^* * Significant at 0.1, 1, and 5% probability, respectively. Comparison was done by using the Dunnett’s test. -, missing data. 118.70^* ***** ,	-0.32	+107.68^* ***** ,
		T2	-0.004	+1.15^* ***** ,	1.37	+263.85^* ***** ,	+0.82^* ***** ,	+265.89^* ***** ,

	Pisang Madu	T0	15.45	18.3	110.65	2871.61	78.48	2276.65
		T1	+1.23^* ***** ,	+1.4^* ***** ,	-0.55^* * Significant at 0.1, 1, and 5% probability, respectively. Comparison was done by using the Dunnett’s test. -, missing data.	+344^* ***** ,	+1.86^* ***** ,	223
		T2	+2.6^* ***** ,	+2.0^* ***** ,	0.43	+1322^* ***** ,	+7.81^* ***** ,	+1255^* ***** ,

	Tomolo	T0	12.07	-	100.37	377	79.52	300
		T1	-0.03	-	-	+53.89^* ***** ,	-0.32	+107.68^* ***** ,
		T2	-0.16^* * Significant at 0.1, 1, and 5% probability, respectively. Comparison was done by using the Dunnett’s test. -, missing data.	-	-	+300.85^* ***** ,	+0.81^* ***** ,	+265.89^* ***** ,

Triploid	Yangambi km5	T0	17.55	27.1	148.35	1515.75	48.88	727.01
		T1	0.23	0.2	+1.15^* ***** ,	+251^* ***** ,	+2.13^* ***** ,	+135^* ***** ,
		T2	0.09	+1.7^* ***** ,	+1.30^* ***** ,	+413^* ***** ,	+5.88^* ***** ,	+309^* ***** ,

Tetraploid	CRBP 39	T0	12.29	24.77	140.11	3911.32	56.85	2173.80
		T1	-7.0^* ***** ,	0.03	-6.48^* ***** ,	499	+13.13^* ***** ,	+909^ ,
		T2	-2.0^* ***** ,	+2.2^* ***** ,	-2.37^* ***** ,	+2060^* ***** ,	-4.26^* ***** ,	+971^* ***** ,

	FHIA 21	T0	14.22	28.97	134.02	8237.51	69.44	5624.34
		T1	-2.0^* ***** ,	-2.9^* ***** ,	-1.33^ ,	-1117^* ***** ,	-0.60	-855^* * Significant at 0.1, 1, and 5% probability, respectively. Comparison was done by using the Dunnett’s test. -, missing data.
		T2	-2.0^* ***** ,	-1.1^* ***** ,	-0.21	+594^* * Significant at 0.1, 1, and 5% probability, respectively. Comparison was done by using the Dunnett’s test. -, missing data.	-0.32	381

Genotype	Number of pollen per anther T0→T2	Effect of total flower suppression
Tomolo	377→ +300	80%	Very high
Mshale	881→ + 270	30%	Medium
Mshare Mrerembo	833→ +237	28%	Medium
Yangambi km5	1,515→ +413	27%	Medium
Pisang Lilin	1,573→ +263	17%	Medium
Pisang Madu	2,871→ +1,322	46%	High
CRBP 39	3,911→ +2,060	53%	High
FHIA 21	8,327→ +594	7%	Low

Genotype	Treatment	Number of cross	Mean seed set per hand						Mean±standard deviation
Genotype	Treatment	Number of cross	Hand 1	Hand 2	Hand 3	Hand 4	Hand 5	Hand 6	Mean±standard deviation
Pisang Lilin	T0	9	49	46	47	48	55	50	49.35±4.38c
	T1	9	60	55	58	59	56	53	56.63±6.67b
	T2	9	65	66	64	68	68	69	66.67±5.00a
Tomolo	T0	9	3	2	3	2	1	2	2.28±0.81c
	T1	9	2	3	4	3	2	3	2.94±1.45b
	T2	9	6	6	7	5	7	6	6.11±1.54a

Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira Caixa Postal 040315, 70770-901 Brasília DF Brazil, Tel. +55 61 3448-1813, Fax +55 61 3340-5483 - Brasília - DF - Brazil
E-mail: pab@embrapa.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] Corresponding author