Serological frequency of <i>Leptospira</i> spp. in buffaloes (<i>Bubalus bubalis</i>) in Paraná state, Brazil

Krueger, Louise; Biondo, Alexander W.; Kmetiuk, Louise B.; Lara, Maria do Carmo C.S.H.; Castro, Vanessa; Dib, Cristina C.; Oliveira, Rodrigo A.M.; Perotta, João H.; Barros Filho, Ivan R.

doi:10.1590/1678-5150-PVB-7147

Acessibilidade / Reportar erro

Brasil

Pesquisa Veterinária Brasileira

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Livestock Diseases • Pesq. Vet. Bras. 43 • 2023 • https://doi.org/10.1590/1678-5150-PVB-7147 copy

Serological frequency of Leptospira spp. in buffaloes (Bubalus bubalis) in Paraná state, Brazil

Frequência sorológica de Leptospira spp. em búfalos (Bubalus bubalis) no estado do Paraná, Brasil

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

Leptospirosis is a zoonosis that can infect buffaloes and other mammalians, and it’s caused by Gram-negative bacteria of the genus Leptospira spp. The infection occurs through contact with urine or a contaminated environment with the mucous membranes of the animals, and the disease has two phases: leptospiremia and leptospiruria. The objective of this study was to determine the frequency of leptospirosis in buffaloes in the state of Paraná, Brazil, as well as the main serovars present in this area. A total of 276 blood samples were collected from buffaloes from 18 properties located in different cities and mesoregions of Paraná state. A microscopic agglutination test (MAT) was performed to detect the presence of anti-Leptospira spp. antibodies and to identify its serovars. The prevalence was 77.17% (213/276) of buffaloes seropositive for at least one serovar. The most frequent serovar was Icterohaemorrhagiae, followed by the Pomona serovar and serovar Hardjo. This study seems to be the first to determine the frequency of buffaloes with leptospirosis in the state of Paraná. Prevention and control measures are important to control the disease in herds.

INDEX TERMS:
Leptospirosis; buffaloes; Bubalus bubalis; Leptospira; seroepidemiology; Icterohaemorrhagiae; Hardjo; Pomona; Brazil

Colégio Brasileiro de Patologia Animal - CBPA Pesquisa Veterinária Brasileira, Caixa Postal 74.591, 23890-000 Rio de Janeiro, RJ, Brasil, Tel./Fax: (55 21) 2682-1081 - Rio de Janeiro - RJ - Brazil
E-mail: pvb@pvb.com.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] *Corresponding author: louisekrueger@hotmail.com

Serovar	Reagent		Not reagent
Serovar	N	%	N	%
Icterohaemorrhagiae	197	92.49%	14	6.49%
Pomona	52	24.41%	161	75.59%
Hardjo	49	23.00%	164	77.00%
Autumnalis	39	18.31%	174	81.69%
Grippotyphosa	30	14.08%	183	85.92%
Bratislava	22	10.33%	191	89.67%
Canicola	3	1.41%	210	98.59%
Pyrogenes	2	0.94%	211	99.06%

MMNH^a		NAH^b	ICT^c	CAN^d	POM^e	GRIP^f	HRJ^g	AUT^h	BRAⁱ	PYR^j
Oriental Center
	Ponta Grossa/herd 1	22	13	-	8	1	7	1	3	1
	Carambeí/herd 2	20	11	-	2	-	-	-	2	-
South Center
	Laranjeira do Sul/herd 3	10	-	-	-	1	-	-	-	-
	Laranjeira do Sul/herd 4	6	1	-	-	-	-	-	-	-
Curitiba (Metropolitan)
	Campina grande do Sul/herd 5	31	29	1	5	-	5	5	5	1
	Adrianópolis/herd 6	8	8	-	-	-	8	2	-	-
	Morretes/herd 7	23	20	-	4	5	3	-	-	-
	Cerro Azul/herd 8	15	11	-	-	2	7	2	-	-
	Cerro Azul/herd 9	11	9	-	1	2	-	4	2	-
	Rio Branco do Sul/herd 10	30	27	-	1	5	15	6	2	-
	Rio Branco do Sul/herd 11	21	19	-	1	2	-	3	-	-
	Rio Branco do Sul/herd 12	26	25	1	21	5	2	9	7	-
	Campo Largo/herd 13	7	7	1	7	4	1	3	1	-
North Center
	Uniflor/herd 14	11	9	-	1	2	-	2	-	-
North Pioneer
	Pinhalão/herd 15	7	-	-	-	-	-	-	-	-
	Ibaiti/ herd 16	8	-	-	-	-	-	-	-	-
Northeast
	São Mateus do Sul/herd 17	14	8	-	1	1	1	2	-	-
	Teixeira Soares/herd 18	6	-	-	-	-	-	-	-	-
TOTAL		276	197	3	52	30	49	39	22	2

MMNH^a	NAH^b	NBPALS^c	PASIP^d	CAS^e	RP^f
Oriental Center
1*	22	18	Dogs	Bovine, horses and sheeps	Yes
2*	20	14	Dogs	Bovine and horses	Yes

South Center
3*	10	1	Dogs	Bovine, horses and sheeps	No
4*	6	1	Dogs	Bovine, horses and sheeps	No

Curitiba
5*	31	29	Dogs and cats	Swine	No
6*	8	8	-	-	No
7*	23	22	Dogs and cats	-	No
8*	15	14	Dogs and cats	Horses, sheeps and swine	No
9*	11	10	Dogs and cats	Horses and swine	Yes
10*	30	28	-	-	No
11*	21	19	Dogs and cats	Horses	No
12*	26	25	Dogs	-	Yes
13*	7	7	Dogs and cats	-	No

North Center
14*	11	9	Dogs and cats	Bovine and horses	Yes

North Pioneer
15*	7	0	Dogs	Bovine, horses and sheeps	Yes
16*	8	0	Dogs	Bovine and horses	No

Northeast
17*	14	8	Dogs and cats	Bovine, horses and sheeps	No
18*	6	0	Dogs and cats	Bovine and horses	No
TOTAL	276	213

		Positive (n=213)		Negative (n=63)		OR (95%)	p-value
		n	%	n	%	OR (95%)	p-value
Presence of dogs on the property	Yes	177	74.37%	61	25.63%	0.16 (0.03-0.69)	0.005
Presence of dogs on the property	No	36	94.74%	2	5.26%	0.16 (0.03-0.69)	0.005
Presence of cats on the property	Yes	118	84.89%	21	15.11%	2.48 (1.38-4.48)	0.002
Presence of cats on the property	No	95	69.34%	42	30.66%	2.48 (1.38-4.48)	0.002
Co-grazing with swine	Yes	53	92.98%	4	7.02%	4.89 (1.69-14.09)	0.001
Co-grazing with swine	No	160	73.06%	59	26.94%	4.89 (1.69-14.09)	0.001
Co-grazing with bovines	Yes	51	49.04%	53	50.96%	0.06 (0.03-0.12)	4.724
Co-grazing with bovines	No	162	94.18%	10	5.82%	0.06 (0.03-0.12)	4.724
Co-grazing with horses	Yes	94	62.25%	57	37.75%	0.08 (0.03-0.20)	7.955
Co-grazing with horses	No	119	95.20%	6	4.80%	0.08 (0.03-0.20)	7.955
Co-grazing with sheeps	Yes	42	56.76%	32	43.24%	0.24 (0.13-0.43)	1.001
Co-grazing with sheeps	No	171	84.65%	31	14.35	0.24 (0.13-0.43)	1.001
Reproductive problems	Yes	76	78.35%	21	21.64%	1.11 (0.61-2.01)	0.732
Reproductive problems	No	137	76.54%	42	23.46%	1.11 (0.61-2.01)	0.732

Number of buffaloes in the herd	Number of herds	Number total in the herds	Positive		Negative
Number of buffaloes in the herd	Number of herds	Number total in the herds	n	%	n	%
6-10	7	52	17	32.69%	35	67.31%
11-20	5	71	55	77.46%	16	22.54%
21-30	5	122	112	91.80%	10	8.20%
31-40	1	31	29	93.55%	2	6.45%
TOTAL	18	276	213	-	63	-