This work aimed to evaluate the in vitro establishment of the species Anadenanthera colubrina var. cebil, throug the morphophysiological answers of the seedlings subjected to several culture conditions. The seeds were desinfested and inoculated in Petri plates containing germtest paper, previously sterilized and imbibed with sterile water. The plates were kept in the dark for two days, until the seeds germinated. Then, they were transferred to the test tube containing WPM medium. Three types of test tube closings were evaluated - PVC film, plastic lid without PVC film and cotton lid. In the second experiment, the culture medium was supplied with activated charcoal (0,0 and 82,2mM) and different sucrose concentrations (0,0; 29,21; 58,43; 87,64mM). After 30 days, highly significant effects of the types of closure were observed on all the variables analysed - aereal part length (APL), number of leaves (NL), number of pinnas (NP), dry matter of aereal part (DMAP), leaf abscission (LA), root length (RL), root dry matter (RDM) and number of secondary roots (NSR). The LA was reduced in 7,3 times when the cotton lid was used instead of the PVC film. The greatest values for APL (102,5 mm), NP (3,0), RL (194,7 mm) and NRS (47,88) were obtained with 29,21mM of sucrose. For DMAP (59,1 mg), when it used 58,43mM, and for RDM (33,37 mg), with 87,64mM of sucrose. The activated charcoal showed significant effects only for the variable SRN, presenting the greatest value (45,79) in the presence of this compound.
Aeration; sucrose; activated charcoal
