The propagation of some superior black wattle individuals (Acacia mearnsii De Wild.) aiming the establishment of more productive, homogeneous forests, resistant to plagues and diseases, is in great need in our days. The objective of the present report is to find the best culture medium and analyze the use of activated charcoal in the in vitro multiplication of axillary buds of A. mearnsii. Explants of in vitro germinated plants were inoculated in different culture media: B5, MS, SP and WPM. Several concentrations of MS medium were tested: 1/4; 1/2; 3/4; 1/1 (original concentration); 5/4; 3/2; 7/4 and 2/1, with and without activated charcoal. It was used a randomized experimental design, with five, seven and four replicates, with four explants per replicate. The evaluations were performed after 30 days, through the counting of the numbers of leafs and axillary shoots and the presence of callus and chlorosis. Among the media used in the original composition, the MS promoted the best multiplication of axillary shoots (3,7 buds/explants) on the thirtieth day of culture. Among the MS medium macro-nutrient concentrations, the dilution to 3/4 of the original concentration with the addition of activated carbon promoted the best responses for the multiplication of black wattle axillary shoots (7,7 buds/explant) after 60 days of culture.
Black wattle; activated charcoal; in vitro micropropagation
