Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs

Class, Camila Souza Carvalho; Fialho, Priscila Alves; Alves, Leucio Câmara; Silveira, Renato Luiz; Amendoeira, Maria Regina Reis; Knackfuss, Fabiana Batalha; Barbosa, Alynne da Silva

doi:10.1590/S1984-29612023013

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Short Communication • Rev. Bras. Parasitol. Vet. 32 (2) • 2023 • https://doi.org/10.1590/S1984-29612023013 linkcopy

Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs

Comparação entre as técnicas de McMaster e Mini-FLOTAC para o diagnóstico de parasitos gastrointestinais de suínos

Authorship SCIMAGO INSTITUTIONS RANKINGS

This purpose of this study was to compare the efficiency of the McMaster and Mini-FLOTAC quantitative techniques in the investigation of helminths in feces of pigs. An analysis was made of 74 fecal samples from pigs raised on family farms located in Rio de Janeiro, Brazil. These were analyzed by the Mini-FLOTAC and McMaster techniques in a solution of 1,200g/mL NaCl. This investigation revealed a superiority in the frequency of all helminths detected by Mini-FLOTAC, including Ascaris suum, Trichuris suis, strongyles and Strongyloides ransomi. The Kappa index revealed substantial agreement in all comparisons made in relation to the frequency of positive samples. However, significant statistical differences in the comparison of EPGs between McMaster and Mini-FLOTAC were observed for all nematodes (p ≤0.05). Higher values of Pearson’s linear correlation coefficient (r), between the techniques in relation to EPG were observed for A. suum and T. suis, differently from what was observed for strongyles and S. ransomi. Mini-FLOTAC proved to be a more satisfactory and reliable technique both for the diagnosis of parasites and for the determination of EPG in pig feces due to the larger size of its counting chambers, thus increasing the helminth egg recovery rates.

Keywords:
Parasitological diagnosis; quantitative techniques; EPG; Nematodes

Parasites	McMaster	Mini-FLOTAC		Kappa (CI 95%)
Parasites	McMaster	Positive	Negative	Kappa (CI 95%)
Ascaris suum	Positive	20	2	0.782 (0.629 - 0.936)
	Negative	5	47	0.782 (0.629 - 0.936)
Trichuris suis	Positive	12	0	0.686 (0.481 - 0.892)
	Negative	8	54	0.686 (0.481 - 0.892)
Strongyloides ransomi	Positive	33	1	0.6547 (0.49 - 0.81)
	Negative	12	28
Strongyles	Positive	62	1	0.7691 (0.63 - 0.94)
	Negative	3	8

Quantitative technique	*Ascaris suum*			*Trichuris suis*			*Strongyloides ransomi*			Strongyles
Quantitative technique	Mean ±SD	CV (%)	Max.	Mean ±SD	CV (%)	Max.	Mean ±SD	CV (%)	Max.	Mean ±SD	CV(%)	Max.
McMaster	988 ± 3090	312.7	18744	37.5 ± 123	328	660	402 ± 1015	252.5	6006	893± 1959	219.4	10989
Mini-FLOTAC	486 ± 1475	303.5	7180	19.5 ± 75.5	387.2	505	404 ± 1512	374.2	12105	696 ± 2508	360.3	20425
Wilcoxon test (p-value)	0.001*			0.035*			0.016*			0.001*

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Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

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[1] CI: Confidence Interval; k = Kappa. Kappa <0 there wasn´t agreement, 0 to 0.20 poor agreement, 0.21 to 0.40 fair agreement, 0.41 to 0.60 moderate agreement, 0.61 to 0.80 good agreement and 0.81 to 1 almost perfect agreement (Landis & Koch, 1977).