Four ruminally cannulated Holstein steers averaging 259 kg of body weight were assigned to a 4x4 Latin square to study the effects of replacing elephantgrass silage with cottonseed hulls on serum urea nitrogen (SUN), ruminal metabolism, urinary excretion of nitrogenous compounds, and microbial protein synthesis measured by omasal purine bases or by urinary excretion of purine derivatives. Treatments (60% of forage) contained on DM basis: 0, 10, 20 or 30% of cottonseed hulls that partially replaced elephantgrass silage in the diet. No significant differences in ruminal pH and concentration of ruminal ammonia were observed by increasing cottonseed hulls from 0 to 30% of the diet DM. However, concentration of both SUN and urinary excretion of urea (mg/kg of BW) decreased linearly whereas the opposite was observed for urinary excretion of purine derivatives and microbial protein synthesis when elephantgrass silage was partially replaced with cottonseed hulls in the diet. Microbial protein synthesis did not differ significantly either measured by omasal purine bases or estimated by urinary excretion of purine derivatives. It can be concluded that cottonseed hulls is a good forage alternative to elephantgrass silage and may supply up to 30% of the total DM in diets of dairy steers.
by-product; omasal collection; purine basis; purine derivatives; roughage; serum urea nitrogen
