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Efficiency of different measurement techniques of sperm concentration in swine

Artificial Insemination (AI) in swines is a rising technique that has been used during the last years at the swine production systems, because it furnish several advantages to the producers just like boar high efficiency, better breeding control and easiness in introducing foreign genetic materials. Accuracy, precision and robustness for ejaculation volume of spermdensimeter (ESPERMIO) and spectrofotometer (ESPECTRO) in relation to the Neubauer Count, designed as the standard technique, were evaluated objectifying higher performance of AI technique. A total of 142 boar ejaculation was used for spermatic concentration measurement by the techniques described above. The average spermatic concentrations (10(6) sptz/mL) and average doses number were, respectively: 229.1 and 22.6, 185.0 and 18.5, 283.6 and 28.0 for Neubauer count, Spectrofotometer and Spermdensimeter. ESPECTRO mean bias (accuracy) and bias standard deviation (precision), in relation to the Neubauer count were, respectively - 44.1 and 52.6, while ESPERMIO were 54.4 and 44.8. Through these results its possible to conclude that ESPECTRO has a tendency to subestimate and ESPERMIO to superestimate the spermatic concentration in relation to the Neubauer Count, and that the ESPECTRO was more accurate and less precise than ESPERMIO. Positive effect of ejaculation volume on accuracy and precision in both techniques (ESPERMIO and ESPECTRO) was confirmed by the robustness results.

accuracy; neubauer count; precision; spectrofotometer; sperm concentration; spermdensimeter


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