The objectives of the present study were to characterize and estimate parameters of kinetics of carbohydrate degradation using alfalfa hay, elephant grass, tifton 85, and corn silage, fed to dairy goats under different forage: concentrate ratio. Kinetic parameters of dry matter and neutral detergent fiber degradation at different pH values were estimated by using cumulative gas production technique and their values contrasted with those obtained by both in vitro and in situ studies. Kinetic interpretation for specific degradability, rate of degradation and the potentially digestible insoluble fraction was done by using the logistic model V(t) = Vf / (1+exp(2+4c(L-T))). A modified McDougall buffer was used for the in vitro assay to adjust pH to the values observed in the animals, by adding 1M of citric acid solution according to the roughage to concentrate ratio studied of 100:0, 80:20, 60:40, 40:60 e 20:80. Similar pattern was found for extent and rate of degradation of the potentially digestible insoluble fraction which confirm the interference of pH on kinetic parameter of carbohydrates in the rumen. Gas production approach yielded the lowest variation coefficient for the values of dry matter degradation but lower figures were found as compared to both in situ and in vitro techniques. Similar values were found for NDF as the three approaches were compared and it was suggested that they can be used interchangeably to precisely estimate NDF degradation.
metabolic technique; fiber degradation
