SUBSTRATE WITH LIGNOCELLULOSIC RESIDUES FOR <i>Pycnoporus sanguineus</i> CULTIVATION

PORTZ, TATIANE MARTINAZZO; MIORANZA, THAISA MURIEL; STANGARLIN, JOSÉ RENATO; KUHN, ODAIR JOSÉ

doi:10.1590/1983-21252022v35n201rc

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Research Paper • Rev. Caatinga 35 (02) • Apr-Jun 2022 • https://doi.org/10.1590/1983-21252022v35n201rc copy

SUBSTRATE WITH LIGNOCELLULOSIC RESIDUES FOR Pycnoporus sanguineus CULTIVATION

SUBSTRATO COM RESÍDUOS LIGNOCELULÓSICOS PARA CULTIVO DE Pycnoporus sanguineus

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Basidiomycete fungi that decompose wood produce substances with promising biological activity for the alternative control of plant diseases. The production of these substances can change according to the climatic conditions and the substrate used for fungal cultivation. The objective of this study was to develop a substrate with sawdust from Eucalyptus sp. and to verify its influence on biomass and cinnabarin production by Pycnoporus sanguineus. Sawdust was used in two particle sizes: less than 500 microns (G1) and between 500–841 microns (G2). Four isolates of P. sanguineus were plated on Petri dishes containing potato broth and agar media added with 0%, 1%, 5%, 10%, and 15% sawdust for each particle size. The largest final diameter of the colony and speed of mycelial growth were observed in the substrate with G1 particle size, with the Ps14 isolate showing the highest averages. For these variables, the sawdust concentration did not influence G1 granulometry and provided the highest values in G2 granulometry. Fresh mycelium mass and cinnabarin production showed the highest values in G2, with the isolated Ps13 and Ps08 showing the highest averages, whereas in G1, Ps14 had the best performance for the analyzed variables. These results indicate that sawdust from Eucalyptus sp., at concentrations of 10% and 15%, is an alternative for the in vitro cultivation of P. sanguineus, and that particle size influences the growth speed, fresh mass production, and cinnabarin content.

Keywords
Basidiomycetes; Fungal growth; Cinnabarin

Granulometries (microns)	N¹ 1 Total nitrogen.	P	K	Cu	Zn	Mn	Fe	C² 2 Organic carbon.	Ratio C/N
Granulometries (microns)	g kg⁻¹			mg kg⁻¹				%	Ratio C/N
< 500	1.75	0.16	1.05	5.00	6.00	82.00	911.00	45.88	262/1
500-841	1.75	0.09	0.90	2.00	5.00	66.00	690.00	42.88	245/1

	Final diameter (cm)
	Granulometries
P. sanguineus isolates	< 500 microns		500 - 841 microns		Means
04	3.58	c A	2.35	b B	2.97	c
08	4.09	a A	2.10	c B	3.10	b
13	3.77	b A	2.38	b B	3.07	bc
14	4.22	a A	2.59	a B	3.41	a
Means	3.91	A	2.36	AB
			CV (%) 8.21

	Growth speed (mm day⁻¹)
	Granulometries
P. sanguineus isolates	< 500 microns		500 - 841 microns		Means
04	4.06	c A	2.86	b B	3.46	c
08	4.83	a A	3.00	b B	3.91	b
13	4.20	b A	2.91	b B	3.56	c
14	5.09	a A	3.26	a B	4.17	a
Means	4.54	A	3.01	AB
			CV (%) 10.38

	Fresh mass of mycelium (mg cm⁻²)
	Granulometries
P. sanguineus isolates	< 500 microns		500 - 841 microns		Means
04	1.51	c B	1.86	bcA	1.68	b
08	1.84	abB	2.58	abA	2.21	a
13	2.15	abB	2.59	acA	2.37	a
14	1.52	bcB	1.94	bcA	1.73	b
Means	1.75	abB	2.24	a A
			CV (%) 24.44

	Cinnabarin content (mg mL⁻¹)
	Granulometries
P. sanguineus isolates	< 500 microns		500 - 841 microns		Means
04	0.77	b B	1.11	ab A	0.94
08	0.54	b A	0.84	b A	0.69
13	0.51	b B	1.94	a A	1.23
14	1.49	a A	1.38	ab B	1.44
Means	0.83	B	1.32	A
			CV (%) 13.67

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