Suckling by <i>Schistosoma mansoni</i>-infected mothers restored IgG2a and TGF-β production, but not IL-6 and delayed-type hypersensitivity in IL-12/IL-23-deficient mice

Silva, Fabiana Leticia da; Silva, Maria da Conceição; Holanda, Gabriela Calixto Ribeiro de; Coutinho, Eridan de Medeiros; Montenegro, Silvia Maria Lucena; Morais, Clarice Neuenschwander Lins de; Souza, Valdênia Maria Oliveira de

doi:10.1590/0037-8682-0744-2020

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Sumário

Short Communication • Rev. Soc. Bras. Med. Trop. 54 • 2021 • https://doi.org/10.1590/0037-8682-0744-2020 linkcopy

Suckling by Schistosoma mansoni-infected mothers restored IgG2a and TGF-β production, but not IL-6 and delayed-type hypersensitivity in IL-12/IL-23-deficient mice

Authorship SCIMAGO INSTITUTIONS RANKINGS

INTRODUCTION Suckling by schistosomotic mice improves anti-ovalbumin (OA) antibody production, while delayed-type hypersensitivity (DTH) remains unaffected. This property of milk from schistosomotic mice was investigated in IL-12/IL-23-deficient mice (IL-12p40KO).

METHODS We compared anti-OA DTH, IgG2a and cytokines in wild-type and IL-12p40KO mice suckled by infected (SIM) or non-infected (CONTROL) mothers.

RESULTS SIM mice showed similar intensity and eosinophils in the DTH, which was abolished in IL-12p40KO and IL-12p40KO-SIM mice. In IL-12p40KO-SIM, IgG2a and TGF-β levels were higher, but IL-6 levels were lower.

CONCLUSIONS Milk from schistosomotic mothers may evoke IgG2a without eliciting DTH in IL-12/IL-23 deficiencies, by changing TGF-β/IL-6 levels.

Keywords:
Schistosomiasis mansoni; Suckling; Hypersensitivity

Groups^a	DTH^b	IgG2a^c	Cytokine (pg/mL)^d
			IFN-γ	IL-17	IL-5	IL-6	TGF-β	IL-10
Control	71±25	0.2±0.3	400±29.8	330±28.0	ND	65±6.0	ND	104±8.6
SIM	94±36	0.5±0.3*	600±10.4	159±8.7*	57±6.0	169±1.8*	55±2.9	144±11
IL-12p40KO	28±15*	0.1±0.01	ND	30±5.5*^#	35±5.2	46±3.9^#	ND	95±9.2
IL-12p40KO SIM	38±19^#	0.4±0.3^δ	ND	23±1.4*^#	52±3.9^δ	24±1.5^#δ	53±3.5	93±4.9

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Sociedade Brasileira de Medicina Tropical - SBMT Sociedade Brasileira de Medicina Tropical - SBMT, Núcleo de Medicina Tropical UnB, Sala 43C 70904-970, E-mails: rsbmt@sbmt.org.br | artes.rsbmt@gmail.com , WhatsApp: SBMT (61) 9.9192-6496, WhatsApp: RSBMT (34) 3317-5855 - Brasília - DF - Brazil
E-mail: rsbmt@sbmt.org.br

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[TFN1] ^a
Wild-type (CONTROL) and IL-12p40-deficient mice (IL-12p40KO) suckled by uninfected mothers and wild-type (SIM) or IL-12p40KO-SIM mice suckled by S. mansoni-infected mothers immunized with OA+CFA. ^bFootpad thickness increases at 24 h in response to OA. The results are presented as mean ±standard deviation. The mice were challenged with aggregated OA in the footpad 8 days after the OA+CFA immunization. ^cOA-specific IgG2a antibody levels 9 days after OA+CFA immunization Plasma titration was performed using ELISA. The results represent the mean OD ±standard deviation of five mice at a 1:32 dilution. ^dCytokine content in the culture supernatants was assayed using two-site sandwich ELISA, and the results represent the mean of duplicate cultures plus standard deviation. The detection limits were 9.4 pg/ml for IFN-γ; 10.9 pg/ml for IL-17; 15.6 pg/mL for IL-5; 7.8 pg/ml for IL-6; 31.2 pg/ml for TGF-β; 15.6 pg/mL for IL-10. Nine days after OA+CFA immunization spleen cells were stimulated with OA (500 mg/mL) for 48 h (5 × 10⁶ cells). ND: non-detected. *Ρ<0.05 compared with CONTROL; ^#P<0.05 compared with SIM; ^δ P<0.05 compared with IL-12p40KO.