Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions

Sarno, Euzenir Nunes; Sampaio, Elizabeth Pereira; Moreira, André Luís; Alvarenga, Fátima de Barros Fonseca

doi:10.1590/S0037-86821987000400004

Abstracts

In the present study, inflammatory cells from human lepromatous lesions were isolated by enzymatic dissociation of tissue. They were maintained in culture up to five days and their morphologic, cythochemicaland functional characteristics were described.

Macrophage; Enzymatic dissociation; Leprosy; Inflammatory cells

Células inflamatórias presentes em lesões Virchowianas humanas foram isoladas do tecido por um processo de digestão enzimática. Elas foram mantidas em cultura por cerca de 5 dias e suas características morfológicas citoquímicas efuncionaisforam descritas.

Macrófago; Dissociação enzimática; Hanseníase; Células inflamatórias

ARTICLES

Isolation and functional characterization of mononuclear phagocytes from human lepromatous lesions

Euzenir Nunes Sarno; Elizabeth Pereira Sampaio; André Luís Moreira; Fátima de Barros Fonseca Alvarenga

ABSTRACT

In the present study, inflammatory cells from human lepromatous lesions were isolated by enzymatic dissociation of tissue. They were maintained in culture up to five days and their morphologic, cythochemicaland functional characteristics were described.

Keywords: Macrophage. Enzymatic dissociation. Leprosy. Inflammatory cells.

RESUMO

Células inflamatórias presentes em lesões Virchowianas humanas foram isoladas do tecido por um processo de digestão enzimática. Elas foram mantidas em cultura por cerca de 5 dias e suas características morfológicas citoquímicas efuncionaisforam descritas.

Palavras-chave: Macrófago. Dissociação enzimática. Hanseníase. Células inflamatórias.

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Recebido para publicação em 28/4/86.

Departamento de Patologia Geral da Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, UERJ, Brasil. Av. 28 de Setembro 87, Fundos 4.° Andar, Vila Isabel, 20551 Rio de Janeiro, RJ.

Trabalho financiado em parte pelo CNPq - Processo n.° 40.0927/85 e pela Universidade Rockefeller.

1. AmsdenAF, Boros DL. Fc-receptor-bearing macrophages isolated from hypersensitivity and foreign-body granulomas. The American Journal of Pathology 96: 457- 473, 1979.
2. Bonney RJ. Obtaining mononuclear phagocytes from granulomas. In: Adams DO, Edelson PJ, Koren H (eds). Methods for studying mononuclear phagocytes, 1 st edition, Academic Press, Inc. New York, pp. 111-119, 1981.
3. Bonney RJ, Gery I, Lin TX, Meyenhofer MF, Acevedo W, Davies P. Mononuclear phagocytes from carrageenan-induced granulomas: Isolation cultivation and characterization. The Journal of Experimental Medicine 148: 261-275, 1978.
4. Bozdech MJ, Baiton DF. Identification of oc-naphtyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane- bounded organelle in lymphocytes. The Journal of Experimental Medicine 153: 182-195, 1981.
5. Chilosi M, Pizzolo G, Menestrina F, Iannucci AM, Bonetti F, Fiore-Donati L. Enzyme histochemistry on normal and pathologic paraffin-embedded lymphoid tissues. American Journal of Clinical Pathology 76: 729- 736, 1981.
6. Lachman PJ, Hobart MJ. Complement Technology. In: Weir DM (ed). Handbook of Experimental Immunology Vol. 1 Immunochemistry, Blackwell, Oxford, 5A, 1-5A, 23, 1978.
7. Russel SW. Obtaining mononuclear phagocytes from disaggregated neoplasms. In: Adams DO, Edelson PJ, Koren H (eds). Methods for studying mononuclear phagocytes, 1st edition, Academic Press, Inc. New York, pp. 103-109, 1981.
8. Russel SW, Doe WF, Hoskins RG, Cochrane CG. Inflammatory cells in solid neoplasms. I. Tumor disaggregation and identification of constituent inflammatory cells. International Journal of Cancer 18: 322-330, 1976.
9. Stadecker MJ, Wiler DJ, Wright JA. Ia antigen expression and antigen-presenting function by macrophages isolated from hypersensitivity granulomas. The Journal of Immunology 128: 2739-2744, 1982.
10. Stadecker MJ, Wright JA. Distribution and kinetics of mononuclear phagocytes in granulomas elicited by eggs of Schistosoma mansoni The American Journal of Pathology 116: 245-254, 1984.
11. Williams GT. Isolated epithelioid cells from disaggregated BCG granulomas - An ultrastructural study. The Journal of Pathology 136: 1-13, 1982.
12. Williams GT. Isolated epithelioid cells from disaggregated BCG granulomas - Some functional studies. The Journal of Pathology 136: 15-25, 1982.
13. Yam LT, Li CY, Crossby NH. Cytochemical identification of monocytes and granulocytes. American Journal of Clinical Pathology 55: 283-290, 1971.

Publication Dates

Publication in this collection
29 May 2013
Date of issue
Dec 1987

History

Accepted
28 Apr 1986
Received
28 Apr 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AmsdenAF, Boros DL. Fc-receptor-bearing macrophages isolated from hypersensitivity and foreign-body granulomas. The American Journal of Pathology 96: 457- 473, 1979.

[2] 2. Bonney RJ. Obtaining mononuclear phagocytes from granulomas. In: Adams DO, Edelson PJ, Koren H (eds). Methods for studying mononuclear phagocytes, 1 st edition, Academic Press, Inc. New York, pp. 111-119, 1981.

[3] 3. Bonney RJ, Gery I, Lin TX, Meyenhofer MF, Acevedo W, Davies P. Mononuclear phagocytes from carrageenan-induced granulomas: Isolation cultivation and characterization. The Journal of Experimental Medicine 148: 261-275, 1978.

[4] 4. Bozdech MJ, Baiton DF. Identification of oc-naphtyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane- bounded organelle in lymphocytes. The Journal of Experimental Medicine 153: 182-195, 1981.

[5] 5. Chilosi M, Pizzolo G, Menestrina F, Iannucci AM, Bonetti F, Fiore-Donati L. Enzyme histochemistry on normal and pathologic paraffin-embedded lymphoid tissues. American Journal of Clinical Pathology 76: 729- 736, 1981.

[6] 6. Lachman PJ, Hobart MJ. Complement Technology. In: Weir DM (ed). Handbook of Experimental Immunology Vol. 1 Immunochemistry, Blackwell, Oxford, 5A, 1-5A, 23, 1978.

[7] 7. Russel SW. Obtaining mononuclear phagocytes from disaggregated neoplasms. In: Adams DO, Edelson PJ, Koren H (eds). Methods for studying mononuclear phagocytes, 1st edition, Academic Press, Inc. New York, pp. 103-109, 1981.

[8] 8. Russel SW, Doe WF, Hoskins RG, Cochrane CG. Inflammatory cells in solid neoplasms. I. Tumor disaggregation and identification of constituent inflammatory cells. International Journal of Cancer 18: 322-330, 1976.

[9] 9. Stadecker MJ, Wiler DJ, Wright JA. Ia antigen expression and antigen-presenting function by macrophages isolated from hypersensitivity granulomas. The Journal of Immunology 128: 2739-2744, 1982.

[10] 10. Stadecker MJ, Wright JA. Distribution and kinetics of mononuclear phagocytes in granulomas elicited by eggs of Schistosoma mansoni The American Journal of Pathology 116: 245-254, 1984.

[11] 11. Williams GT. Isolated epithelioid cells from disaggregated BCG granulomas - An ultrastructural study. The Journal of Pathology 136: 1-13, 1982.

[12] 12. Williams GT. Isolated epithelioid cells from disaggregated BCG granulomas - Some functional studies. The Journal of Pathology 136: 15-25, 1982.

[13] 13. Yam LT, Li CY, Crossby NH. Cytochemical identification of monocytes and granulocytes. American Journal of Clinical Pathology 55: 283-290, 1971.