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Variability of Corynespora cassiicola (Berk. & Curt.) Wei isolates from Amazonas, in culture media

Corynespora cassiicola, responsible for economic losses in dozens of commercial species in the Amazon region, is the main pathogen affecting the shoot of tomato (Solanum lycopersicum L.). This study evaluated the morphological characteristics of C. cassiicola isolated from three hosts in Amazonas. The variability and the morpho-culture of C. cassiicola isolates were studied in the culture media Potato Dextrose Agar (PDA), Potato Sucrose Agar (PSA), Oatmeal Agar (VLE) and Carrot Agar (CA). Disks of 5 mm diameter, taken from colonies grown on PDA medium for 15 days, were transferred to the center of Petri dishes containing the culture media and maintained at room temperature (± 26 ºC) under continuous light. Treatments were arranged in a 4 x 15 factorial (isolates x culture media) with ten replicates. The highest mycelial growth rate occurred in CA medium and the highest sporulation in the media PDA and PSA for isolates from tomato and cucumber. The aspect of colonies varied in color between isolates from the same host and between isolates from different hosts for all culture media. The size of conidia ranged from 41.40 to 81.20 µm in length by 4.30 to 6.95 µm in width and showed 1 to 14 pseudosepta.

Corynespora spot; mycelial growth; sporulation


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