Método de separação e determinação das bases purinas cafeína e teobrominaNOTAS
Método de separação e determinação das bases purinas cafeína e teobromina
A method for the separation and determination of the purine bases: caffeine and theobromine
Dayse Soave Spoladore; João Paulo F. Teixeira
Seção de Fito química, Instituto Agronômico, IAC
SUMMARY
A method for separation and determination of caffeine and theobromine in plant material by chromatographic column is presented.
The alkaloids were extracted from the plant material (cocoa bean) by MgO powder, and separated in chromatographic column using alumina (A12O3) as support and the solvent was 5% butanol in water washed chloroform. The compound present in the effluent was determinated by spectrofotometry at 276.5nm.
The results obtained were considered satisfactory for both, standard and plant material alkaloids solutions.
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Recebida para publicação a 20 de outubro de 1979.
Datas de Publicação
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Publicação nesta coleção
21 Dez 2007 -
Data do Fascículo
1980
