Growth of Nile tilapia Oreochromis niloticus fed with different levels of alcohol yeast

Crescimento das tilápias do Nilo Oreochromis niloticus alimentadas com diferentes níveis de levedura alcooleira

Growth of Nile tilapia Oreochromis niloticus fed with different levels of alcohol yeast

MEDRI, V.,¹ PEREIRA, G. V.² and LEONHARDT, J. H.³

¹ Departamento de Matemática Aplicada, Universidade Estadual de Londrina, Campus Universitário,

CEP 86051-990, Londrina, PR, Brazil

² Departamento de Química, Universidade Estadual de Londrina, Campus Universitário, CEP 86051-990, Londrina, PR, Brazil

³ Departamento de Biologia Animal e Vegetal, Universidade Estadual de Londrina, Campus Universitário, CEP 86051-990, Londrina, PR, Brazil

Correspondence to: Vandir Medri, Rua Santos, 267, apto. 502, Centro, CEP 86020-020, Londrina, PR, Brazil, e-mail: [e-mail]

Received October 21, 1998 – Accepted March 1, 1999 – Distributed February 28, 2000

(With 1 figure)

ABSTRACT

Two hundred and forty 45-day-old fingerlings of Nile tilapia (Oreochromis niloticus), sexually reverted with an initial average weight of 1.25 ± 0.14 g, distributed in a totally casualized delineation, during 330 days. The effect of the substitution of 10%, 20% and 30% of the ration by distillery yeast (Saccharomiyces cerevisae) in balanced experimental rations on the development in the breeding of tilapias did not show a harmful effect up to the maximum tested level of 30%, showing that the choice of the yeast in the ration for these fishes depends on the availability and occasional cost. The yeast provides fish with good resistance to unfavorable environmental conditions, which could be verified since there were no statistical differences among the treatments with different levels of yeast and the witness. The long growth rate in terms of length and weight can be attributed to the little available space for each specimen, not obeying the limit of populational density, as well as reduction of food ingestion, which coincide with the winter season, above of the absence of natural feeding.

Key words: yeast, Oreochromis niloticus, ration, tilapia.

RESUMO

Crescimento das tilápias do Nilo Oreochromis niloticus alimentadas com diferentes níveis de levedura alcooleira

Foram utilizados 240 alevinos de tilápia do Nilo (Oreochromis niloticus), com 45 dias, sexualmente revertidos com peso médio inicial de 1,25 ± 0,14 g, distribuídos em um delineamento inteiramente casualizado, durante 330 dias. O desempenho da substituição de 10%, 20% e 30% da ração por levedura de destilaria (Saccharomyces cerevisiae) em rações experimentais balanceadas sobre o desenvolvimento na criação de tilápia do Nilo não mostrou efeito prejudicial até o nível máximo testado de 30%, indicando que a escolha do nível de levedura na ração para estes peixes depende de sua disponibilidade e custo ocasional. A levedura proporcionou aos peixes boa resistência às condições ambientais desfavoráveis, o que foi constatado pela não diferença estatística entre os tratamentos com diferentes níveis de levedura e o controle. O baixo índice de crescimento em comprimento e peso pode ser atribuído ao pouco espaço disponível por exemplar, não obedecendo o limite de densidade populacional, e uma diminuição da ingestão de alimentos coincidindo com o período de inverno, além da ausência de alimentação natural.

Palavras-chave: levedura, Oreochromis niloticus, ração, tilápias.

INTRODUCTION

Yeast is a worth product, adequately balanced, withdrawn from the process of alcoholic fermentation, and is an important alternative of protein on formulation of animal ration, so that high levels of protein, carbohydrates, lipids, etereo extract, vitamins and minerals are obtained (Mattos, 1984; Medri, 1997).

The Brazilian population eats deficiently in terms of quantity and quality, therefore there is necessity to develop new ways of using the industrial residues, transforming them into protein when used in the animals diet, besides the reduction on the costs and the minimization of the pollutant force of these residues in rivers and lakes (Neto et al., 1988).

Unicellular protein or yeast could be used as proteic supplement in animal rations as well as in enrichment of school lunch, on account to the low rate of protein contained on the diet of most part of the population (Carneiro, 1971).

It is necessary effort to convince the industrials of the quality of a sugarcane subproduct, such as the yeast withdrawn from the fermentation process. Yeast is a worth product, but its importance is not recognized in the marked, so the industries are not stimulated to produce it.

The improving necessity in quality and quantity of food, bring the industrials and scientists to work harder on the progress of the production, through advanced methods that maximize the efficiency when using natural, human, technological and economical resources but not degrading the environment.

Tilapias present excellent characteristics, such as rusticity, fast increase of weight and ability to make use of agroindustrial residues such as yeast, besides the possibility of assimilate efficiently carbohydrates from the vegetable ingredients of rations.

Young tilapia eat mainly zooplancton and phitoplancton; while the adults, accept a variety of artificial food, vegetables, larvae and insects (Castagnolli, 1992; Wu et al., 1995).

The objective of this article is to show through statistic the inclusion of 10%, 20% and 30% of distillery yeast in experimental rations and the relative growth of the fishes in weight and length.

MATERIAL AN METHODS

Experimental conditions

Two hundred and forty fingerlings of Nile tilapia (Oreochromis niloticus), ceded by the Fish Breeding Station of the Animal and Vegetal Department of Biology Science Center of the Universidade Estadual de Londrina. The initial average weight and length of the fingerlings were respectively 1.25 ± 0.14 g and 3.84 ± 0.17 cm. The fishes were reverted by the supply of rations with 60 mg/Kg of diet of the male hormone 17a –metiltestosterone, during a period of 30 days.

A computational program was utilized to elaborate the ration BRUN10 with the needs of the mentioned species. The four isoproteic (28% PB) and isocaloric balanced rations (2,933 Kcal/kg) with 0% (standard group), 10%, 20% and 30% (tested groups) of yeast from alcoholic distillery exceeding (Table 1).

Each of the rations treatment named (T) was given to three groups of fishes (triplicate). The fingerlings were daily fed according to Wilson (1991).

The fingerlings were randomly distributed in 12 groups of 20 individuals, each group was placed in an amianthus box with capacity for 500 liters, with continuos aeration and water exchange. They were supplied with water from a semi-artesian well, with discharge of 6 liters/second/hectare and placed in a closed environment.

The box were siphoned weekly to remove residues and algae deposited on the bottom and on the walls. The fishes were daily observed in case of any uncommon behavior, morphological variation and death. The experimental period was 330 days (15/03/95 to 15/02/96). Using a paquimeter and a balance of precision, the total weight (Wt), in grams, and the total length of the fishes (Lt), in centimeters, were monthly measured.

The water temperature was checked every day with a mercury thermometer. Monthly, the alkalinity was measured through the addition method, the dissolved oxygen through Winkler method, ammonia through photometer method of Berthelot, nitrite through Griess-Hosvay, total phosphorus and dissolvable phosphorus through Murphy and pH through potentiometer. The methodology used to test these parameters was according to Lind (1979) and Standard Methods (1980).

The finality of this control is to determinate the quality and quantity of water, which is essential on the interpretation of the results of this research. Thus, it is possible to attribute any dispersion on the fish growth only to the variation of ration applied.

Quantitative analysis

The development of the tilapias related to the different treatments was analyzed through the quantitative analysis of the relation total weight/ total length (Santos, 1978), total biomass, efficiency index (Swingle, 1961; Ivlev, 1966), increment in length and weight, increase of absolute weight and total relative weight, graphical expression of growth in length and weight (Ricker, 1979), graphical expressions of biomass and production (Webb, 1978; Ricker, 1979).

RESULTS AND DISCUSSION

Tilapias growth

The results for the average total length and weight of the standard group (T₁) and the tested groups (T₂, T₃ and T₄) of tilapias are presented on Table 2.

The low index of growth, length and weight (Table 2 and Fig. 1) in the amianthus box can be associated to the absence of natural feeding, besides the little space per fish, that do not follows the population density limits, which according to Coda (1996), have great influence on the growth index of the fishes.

Physical-chemist Analysis of the Water in the Amianthus Boxes

The average values obtained for the physical-chemical variables are in the zone considered ideal for fish breeding, according to Tavares (1994). The rate of water change was kept high during the experimental period, so that the values obtained for the physical-chemical variables of the water did not represent significant statistic difference (P < 0,05) among the treatments T₁, T₂, T₃ and T₄.

The Tukey's test for the average of the physical-chemical analysis of the water in each treatment are on Table 3.

The most important variables that must be controlled in fish breeding, after Boyd (1990), are: temperature, alkalinity, dissolved oxygen, ammonia, nitrite, phosphorus and pH.

The Table 4 presents the results of the cubic regression of the weight related to the months of the biometrics.

On Table 4, the high values for the determination coefficient (r²) shows a perfect adjust of the studied variables to the mathematical model used. On the other side, the variation coefficient values (CV) lower than 10% characterize homogeneity of the results, representative average and optimum experiment. These results showed the possibility of using up to 30% of distillery yeast in the tilapias ration without influence in its growth.

Weight/length relation

The constant value related to the growth form of the fish bodies (q) was near to 3,0. After Wootton (1990), the value q = 3,0 indicates a isometric growth. A value higher than 3,0 means that the fish became lighter on account to its growth in length. A value lower than 3,0 indicates that the fish became heavier because of the weight increase. Values close to these were found by Mainardes Pinto (1989), who worked with tilapias (O. niloticus) growth.

The condition factor (f) is very used in studies of fishery biology, indicating the well being degree of the fishes in the environment they live to verify if they make good use of the ration (Weatherley & Gill, 1987).

The mathematical expressions of the curves of Weight/Total Length, Bodies Height/Total Length, Standard Length/Total Length and Barrel Length/Total Length of tilapias and the linear transformation correspondent to the treatments T₁, T₂, T₃ and T₄ are on Table 5.

The estimated values of the coefficient of linear correlation of Pearson are positive and near to 1 (one), which indicates the high association degree among the variables of the logarithmic expressions, proving analytically the validity of the adjustment expressions corroborating with the little dispersion observed of the empiric points to the theoretic curves.

Growth curve of length and weight

In this article, as there were no possibility to calculate the growth curves of length and weight of the fishes in the amianthus box by the expression of Von Bertalanffy (1938), because there were no satisfactory adjust to the experiments information. So it was choosed the experimental model described by Ricker (1979); Weatherley & Gill (1987); Melo (1993), see Table 6.

The mathematical expressions used in the study of length growth were obtained from the exponential model of curve of weight growth.

Biomass and production curves

The mathematical expressions obtained through the exponential model for biomass and production are presented on Table 7.

Although fishery is a millenary activity, the scientific studies to control and intensify the production in biomass is relatively recent. The study of the aquatic growing intend to explore more rationally the aquiculture, on account to obtain information about a higher or lower productivity of the species bred in the same environmental conditions, the optimum number of fish stocked and maximize the biomass in short time (Melo, 1993).

The expression of the curve of biomass and production were determined with knowledge of the mathematical expression of the curve of growth in weight and the survival rate.

Contribution of the experimental rations in the studied parameters

The analysis of the final results of the experiment (Table 8) show that the final length, the daily increase of length and weight, feed conversion coefficient, efficiency and survival did not present significant statistic differences (P < 0.05) among the treatments.

Although the final weight did not present significant statistic differences (P < 0.05) among the treatments, the treatment with T₄= 30% of distillery yeast presented the highest average.

The final biomass of the fishes presented significant differences (P < 0.05) among the treatments, being T₁ and T₄ higher than T₂ and T₃.

The highest increase of absolute weight was 160,3g in treatment T₄ = 30%, statistically higher (P < 0.01) than the others treatments T₁ = 0%, T₂ = 10% and T₃ = 20%, which did not present differences from each other.

The highest increase of relative weight was 12942 g in treatment T₃, statistically higher (P < 0.01) than T₁ (11,852 g) and T₄ (11,787 g), which did not differ from each other, but were higher than the treatment T₂ (10,537 g).

Averages followed by distinct capital letters differ from each other at 5% of probability level (P < 0.05) and the ones followed by distinct small letters differ from each other at 1% of probability level (P < 0.01).

The yield of the inclusion of distillery yeast (Saccharomyces cerevisiae) in balanced experimental rations related to the development of the fishes did not show damage effect until the maximum level tested of 30%, which shows that the choice of yeast level in rations for fish breeding depends only of its availability and occasional cost.

The feed efficiency (Ea) is the inverse of the feed conversion (S), which means increase of weight/ food consumed (Chiu, 1989). Efficiency refers to the ability of the animal to convert the feed consumed in eatable products or others products (Webb, 1978; Devendra, 1989). The reduction on the growth rate was followed by an increase in the feed efficiency.

Some author cited in literature, who worked with distillery yeast in fish breeding, came to these conclusions:

Ribeiro et al. (1996), who worked with Nile tilapia objecting to analyze the availability of the inclusion of increasing levels of sugarcane yeast, (18%, 36%, 54%, 72%, 90%) included in the ratons in a period of 45 days.

Pezzato et al. (1982), cited by Castagnolli (1992), substituted gradually meat flour by dry yeast at levels of A = 0%, B = 33.3%, C = 66.6% and D = 100% and concluded that the treatments that received yeast were superior to the testimony and that the substitution of 33.3% of yeast was statistically the one that propitiated the best result.

For Alves et al. (1988) the optimum level of substitution of soybean flour by yeast (Saccharomyces cerevisiae) for an increase of weight in Nile tilapias were 36.97%. The results obtained are according to Cowey (1974), when compared the nutritional worth of the yeast, and Matty et al. (1978), who attributed to the yeast the digestibility the proteic matter closed to the observed with soybean flour and that rates higher than 40% of yeast decrease the development of trout.

Pádua (1996), tested five levels (0%, 25%, 50%, 75% and 100%) of substitution of dry fish flour by distillery yeast as proteic supplement and observed that until levels of 75% of substitution did not show damage effect in the productive development and in the metabolism of young pacu.

After Roberts et al. (1988), deficient proteic supply determine a limited growth of the fishes, which can cause some symptoms such as erosion in the dorsal flipper, abnormalities in the vertebral column and darkening of the skin. Waagbo (1994) commented the importance of nutrients in diet on general metabolism and on the animal immunity, suggesting that a good knowledge of preparation of the feed improve the conditions in the breeding, reducing the losses by death.

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Publication Dates

• Publication in this collection
  21 Aug 2000
• Date of issue
  Feb 2000

History

• Received
  21 Oct 1998
• Accepted
  01 Mar 1999