Antibody to human T-lymphotropic virus in a patient with Guillain-Barré syndrome (case report)

Nakauchi, C.M.; Linhares, A.C.; Gomes, M.L.C.; Maruyama, K.; Kanzaki, L.I.; Azevedo, V.N.

doi:10.1590/S0036-46651991000400015

Abstracts

Serum sample obtained from a male, 12 year old patient suffering from Guillain-Barré syndrome (GBS) was positive for human T-lymphotropic virus (HTLV-I) antibody by the enzyme-linked immunosorbent assay (ELISA) and the Western Blot analysis (WB). Attempts to isolate enteroviruses (including poliovirus) from faecal material in both tissue culture and suckling mice were unsuccessful; in addition, acute and convalescent paired serum samples did not show any evidence of recent poliovirus infection when tested against the three serotypes. Specific tests for detection of Epstein-Barr virus infection were not performed; however, the Paul-Bunnel test yielded negative results. ELISA for detection of anti-cytomegalovirus IgM was also negative. The concomitant occurrence of either adult T cell leukemia (ATL) or lymphoma was not recorded in this case.

HTLV-I; Antibody; Guillain-Barré syndrome

Amostra de soro obtida de paciente com a síndrome de Guillain-Barré revelou-se positiva quanto à presença de anticorpos para o vírus linfotrópico humano T (HTLV-I) pelo método imuno-enzimático (ELISA) e a análise por "Western-Blot". Resultaram negativos os testes visando à detecção de enterovírus (incluindo poliovírus) a partir de material fecal, tanto em cultura de tecidos como em camundongos recém-nascidos; exames com amostras de soro aguda e convalescente não exibiram qualquer evidência de infecção recente pelos três tipos de poliovírus. O teste de Paul-Bunnel, assim como o "ELISA" para a detecção de IgM anti-citomegalovírus resultaram negativos. Não foi registrada, no presente caso, quer a leucemia adulta de células T, quer linfomas.

CASE REPORTS

Antibody to human T-lymphotropic virus in a patient with Guillain-Barré syndrome (case report)

Anticorpo para o vírus linfotrópico humano T em um paciente com a síndrome de Guillain-Barré

C.M. Nakauchi^I; A.C. Linhares^I; M.L.C. Gomes^I; K. Maruyama^II; L.I. Kanzaki^III; V.N. Azevedo^III

^ISeção de Vírus do Instituto Evandro Chagas, Fundação Nacional de Saúde. Belém, Pará, Brazil

^IIDepartment of Pathology. Chiba Cancer Center Research Institute, Chiba, Japan

^IIIDisciplina de Virologia, Departamento de Patologia, Universidade Federal do Pará. Belém, Pará, Brazil

^{Address for correspondence} Address for correspondence: Dr. A.C. Linhares Seção de Vírus, Instituto Evandro Chagas Av. Almirante Barroso, 492 CEP 66.050 - Belém, Pará, Brasil

SUMMARY

Serum sample obtained from a male, 12 year old patient suffering from Guillain-Barré syndrome (GBS) was positive for human T-lymphotropic virus (HTLV-I) antibody by the enzyme-linked immunosorbent assay (ELISA) and the Western Blot analysis (WB). Attempts to isolate enteroviruses (including poliovirus) from faecal material in both tissue culture and suckling mice were unsuccessful; in addition, acute and convalescent paired serum samples did not show any evidence of recent poliovirus infection when tested against the three serotypes. Specific tests for detection of Epstein-Barr virus infection were not performed; however, the Paul-Bunnel test yielded negative results. ELISA for detection of anti-cytomegalovirus IgM was also negative. The concomitant occurrence of either adult T cell leukemia (ATL) or lymphoma was not recorded in this case.

Key words: HTLV-I; Antibody; Guillain-Barré syndrome

RESUMO

Amostra de soro obtida de paciente com a síndrome de Guillain-Barré revelou-se positiva quanto à presença de anticorpos para o vírus linfotrópico humano T (HTLV-I) pelo método imuno-enzimático (ELISA) e a análise por "Western-Blot". Resultaram negativos os testes visando à detecção de enterovírus (incluindo poliovírus) a partir de material fecal, tanto em cultura de tecidos como em camundongos recém-nascidos; exames com amostras de soro aguda e convalescente não exibiram qualquer evidência de infecção recente pelos três tipos de poliovírus. O teste de Paul-Bunnel, assim como o "ELISA" para a detecção de IgM anti-citomegalovírus resultaram negativos. Não foi registrada, no presente caso, quer a leucemia adulta de células T, quer linfomas.

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ACKNOWLEDGEMENTS

The technical advice provided by Ms. Cereja Kazuko Nakauchi, Mrs. Laura Nakauchi and Ms. Euda Galiza is gratefully acknowledged.

Recebido para publicação em 07/11/1990

Aceito para publicação em 15/01/1991

References

1. ANDRADA-SERPA, M.J.; DOBBIN, J.A.; GOMES, P.; LINHARES, D.; AZEVEDO, J.G.; HENDRICKS, J.; CLAYDEN, S.A.; RUMJANEK, V.M. & TEDDER, R.S. - Incidence of retroviruses in some Brazilian groups. Immunol. Lett., 18: 15-18, 1988.
2. BURRELLS, C. & DAWSON, Mc L. - ELISA methodology: Variations in technical procedures. In: WARDLEY, R.C., & CROWTHER, J.R. - The ELISA: Enzyme-linked immunosorbent assay in veterinary research and diagnosis. The Hague, Martinus Nijhoff, 1982. p. 1-9.
3. MARUYAMA, K.; KAWAMURA, K.; MIYAUCHI, M.; MOCHIZUKI, S.; FUKUSHIMA, T.; TAKAGI, T. & OGURO, M. - Establishment of cell lines producing both type C virus and herpestype virus and derived from adult T - cell leukemia. Proc. Jap. Cancer Ass., 41: 342, 1982.
4. MARUYAMA, K.; MIYAUCHI, M.; FUKUSHIMA, T.; MOCHIZUKI, S.; KOSHIKAWA, N.; KAWAMURA, K.; TAMAYAMA, C.; NAKANO, M.; ODO, A. & IHA, S. - Antigenic variations of retrovirus in Japanese cancer patients. Leukemia, 3: 277-281, 1989.
5. MATTOCK, C. & PARKER, N.E. - HTLV-I infection and schizophrenia. Lancet, 2: 945, 1985.
6. MERINO, F.; ROBERT-GUROFF, M.; CLARK, J.; BIONDO-BRACHO, M.; BLATNNER, W.A. & GALLO, R.C. - Natural antibodies to human T - cell leukemia/lymphoma virus in healthy Venezuelan populations. Int. J. Cancer, 34: 501-506, 1984.
7. NAKAUCHI, C.M.; LINHARES, A.C.; MARUYAMA, K.; KANZAKI, L.I.; MACEDO, J.E.; AZEVEDO, V.N. & CASSEB, J.S.R. - Prevalence of human T - cell leukemia - virus -I (HTLV-I) antibody among populations living in the Amazon region of Brazil. Mem. Inst. Oswaldo Cruz, 85: 29-33, 1990.
8. OSAME, M.; USUKU, K.; IZUMO, S.; IJICHI, N.; AMITANI, H.; IGATA, A.; MATSUMOTO, M. & TARA, M. - HTLV-I associated myelopathy, a new clinical entity. Lancet, 1: 1031-1032, 1986.
9. POIESZ, B.J.; RUSCETTI, F.N.; GAZDAR, A.F.; BUNN, P.A.; MINNA, J.D. & GALLO, R.C. - Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T - cell lymphoma. Proc. nat. Acad. Sci. (Wash.), 77: 7415-7419, 1980.
10. ROMAN, G.C.; SPENCER, P.S.; SCHOENBERG, B.S.; MADDEN, D.; SEVER, J.; HUGON, J. & LUDOPH, A. - Tropical spastic paraparesis: HTLV-I antibodies in patients from the Seychelles. New Engl. J. Med., 316: 51, 1987.
11. SCHOOLEY, R.T. & DOLIN, R. - Epstein-Barr virus (infectious mononucleosis). In: MANDELL, G.L., DOUGLAS JR., R.G. & BENNETT, J.E. ed. Principles and practice of infectious diseases. New York, John Wiley & Sons, 1979. p. 1324-1341.
12. YOSHIDA, M.; MIYOSHI, I. & HINUMA, Y. - Isolation and characterization of retrovirus from cell lines of human adult T - cell leukemia and its implication in the disease. Proc. nat. Acad. Sci. (Wash.), 79: 2031-2035, 1982.

Address for correspondence:

Dr. A.C. Linhares

Seção de Vírus, Instituto Evandro Chagas

Av. Almirante Barroso, 492

CEP 66.050 - Belém, Pará, Brasil

Publication Dates

Publication in this collection
12 Sept 2006
Date of issue
Aug 1991

History

Received
07 Nov 1990
Accepted
15 Jan 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ANDRADA-SERPA, M.J.; DOBBIN, J.A.; GOMES, P.; LINHARES, D.; AZEVEDO, J.G.; HENDRICKS, J.; CLAYDEN, S.A.; RUMJANEK, V.M. & TEDDER, R.S. - Incidence of retroviruses in some Brazilian groups. Immunol. Lett., 18: 15-18, 1988.

[2] 2. BURRELLS, C. & DAWSON, Mc L. - ELISA methodology: Variations in technical procedures. In: WARDLEY, R.C., & CROWTHER, J.R. - The ELISA: Enzyme-linked immunosorbent assay in veterinary research and diagnosis. The Hague, Martinus Nijhoff, 1982. p. 1-9.

[3] 3. MARUYAMA, K.; KAWAMURA, K.; MIYAUCHI, M.; MOCHIZUKI, S.; FUKUSHIMA, T.; TAKAGI, T. & OGURO, M. - Establishment of cell lines producing both type C virus and herpestype virus and derived from adult T - cell leukemia. Proc. Jap. Cancer Ass., 41: 342, 1982.

[4] 4. MARUYAMA, K.; MIYAUCHI, M.; FUKUSHIMA, T.; MOCHIZUKI, S.; KOSHIKAWA, N.; KAWAMURA, K.; TAMAYAMA, C.; NAKANO, M.; ODO, A. & IHA, S. - Antigenic variations of retrovirus in Japanese cancer patients. Leukemia, 3: 277-281, 1989.

[5] 5. MATTOCK, C. & PARKER, N.E. - HTLV-I infection and schizophrenia. Lancet, 2: 945, 1985.

[6] 6. MERINO, F.; ROBERT-GUROFF, M.; CLARK, J.; BIONDO-BRACHO, M.; BLATNNER, W.A. & GALLO, R.C. - Natural antibodies to human T - cell leukemia/lymphoma virus in healthy Venezuelan populations. Int. J. Cancer, 34: 501-506, 1984.

[7] 7. NAKAUCHI, C.M.; LINHARES, A.C.; MARUYAMA, K.; KANZAKI, L.I.; MACEDO, J.E.; AZEVEDO, V.N. & CASSEB, J.S.R. - Prevalence of human T - cell leukemia - virus -I (HTLV-I) antibody among populations living in the Amazon region of Brazil. Mem. Inst. Oswaldo Cruz, 85: 29-33, 1990.

[8] 8. OSAME, M.; USUKU, K.; IZUMO, S.; IJICHI, N.; AMITANI, H.; IGATA, A.; MATSUMOTO, M. & TARA, M. - HTLV-I associated myelopathy, a new clinical entity. Lancet, 1: 1031-1032, 1986.

[9] 9. POIESZ, B.J.; RUSCETTI, F.N.; GAZDAR, A.F.; BUNN, P.A.; MINNA, J.D. & GALLO, R.C. - Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T - cell lymphoma. Proc. nat. Acad. Sci. (Wash.), 77: 7415-7419, 1980.

[10] 10. ROMAN, G.C.; SPENCER, P.S.; SCHOENBERG, B.S.; MADDEN, D.; SEVER, J.; HUGON, J. & LUDOPH, A. - Tropical spastic paraparesis: HTLV-I antibodies in patients from the Seychelles. New Engl. J. Med., 316: 51, 1987.

[11] 11. SCHOOLEY, R.T. & DOLIN, R. - Epstein-Barr virus (infectious mononucleosis). In: MANDELL, G.L., DOUGLAS JR., R.G. & BENNETT, J.E. ed. Principles and practice of infectious diseases. New York, John Wiley & Sons, 1979. p. 1324-1341.

[12] 12. YOSHIDA, M.; MIYOSHI, I. & HINUMA, Y. - Isolation and characterization of retrovirus from cell lines of human adult T - cell leukemia and its implication in the disease. Proc. nat. Acad. Sci. (Wash.), 79: 2031-2035, 1982.