Pattern of mycobacterial antigen detection in leprosy

Pagliari, C.; Duarte, M. I. S.; Sotto, M. N.

doi:10.1590/S0036-46651995000100002

Abstracts

Immunohistochemistry reaction (Peroxidase anti-peroxidase - PAP) was carried out on fifty-two skin biopsies from leprosy patients with the purpose to identify the antigenic pattern in mycobacteria and to study the sensitivity of this method. Five different patterns were found: bacillar, granular, vesicular, cytoplasmatic and deposits, classified according to the antigenic material characteristics. Deposits (thinely particulate material) appeared more frequently, confirming the immunohistochemistry sensitivity to detect small amounts of antigens even when this material is not detected by histochemical stainings.

Immunohistochemistry; Leprosy; Mycobacterial antigen

Foi realizada reação imunohistoquímica (Peroxidase anti-peroxidase-PAP) em 52 biópsias de pele de pacientes com hanseníase com o objetivo de verificar quais os padrões antigênicos das micobactérias e estudar a sensibilidade deste método como auxílio diagnóstico da hanseníase. Foram encontrados cinco padrões diferentes, classificados como bacilar, granular, vesicular, citoplasmático e depósitos, de acordo com a característica do material antigênico. Os depósitos (material finamente particulado) apareceram com maior frequencia confirmando a sensibilidade da imunohistoquímica na detecção de pequenas quantidades de antígeno, cujo material não é mais visto por colorações histoquímicas usuais.

MICROBIOLOGY

Pattern of mycobacterial antigen detection in leprosy

Detecção de padrões antigênicos de micobactérias na hanseníase

C. Pagliari; M. I. S. Duarte; M. N. Sotto

Lab. Path. Infect. Dis. Univ. of São Paulo Medical School, São Paulo, Brazil

^{Correspondence to} Correspondence to: Profa. Dra. Maria Irma Seixas Duarte Faculdade de Medicina da USP, Depto. de Patologia Av. Dr. Arnaldo, 455 - 1º andar sala 26 01246-903 S. Paulo, SP, Brasil Telephone/FAX: (55)(11) 881-7799

SUMMARY

Immunohistochemistry reaction (Peroxidase anti-peroxidase - PAP) was carried out on fifty-two skin biopsies from leprosy patients with the purpose to identify the antigenic pattern in mycobacteria and to study the sensitivity of this method. Five different patterns were found: bacillar, granular, vesicular, cytoplasmatic and deposits, classified according to the antigenic material characteristics. Deposits (thinely particulate material) appeared more frequently, confirming the immunohistochemistry sensitivity to detect small amounts of antigens even when this material is not detected by histochemical stainings.

Keywords: Immunohistochemistry; Leprosy; Mycobacterial antigen.

RESUMO

Foi realizada reação imunohistoquímica (Peroxidase anti-peroxidase-PAP) em 52 biópsias de pele de pacientes com hanseníase com o objetivo de verificar quais os padrões antigênicos das micobactérias e estudar a sensibilidade deste método como auxílio diagnóstico da hanseníase.

Foram encontrados cinco padrões diferentes, classificados como bacilar, granular, vesicular, citoplasmático e depósitos, de acordo com a característica do material antigênico.

Os depósitos (material finamente particulado) apareceram com maior frequencia confirmando a sensibilidade da imunohistoquímica na detecção de pequenas quantidades de antígeno, cujo material não é mais visto por colorações histoquímicas usuais.

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ACKNOWLEDGEMENTS

This work was supported by LIM/72-HC/FMUSP.

Recebido para publicação em 22/06/1994.

Aceito para publicação em 19/10/1994.

References

1. BARBOLINE, G.; BISETTI, A.; COLIZZI, V. et al. - Immunohistology analysis of mycobacterial antigens by monoclonal antibodies in tuberculosis and mycobacteriosis. Hum. Path., 20: 1078-1083, 1989.
2. HARBOE, M; MSHANA, R.N.; CLOSS, O.; KRONVALL, G. & AXELSEN, N.H. - Cross-reaction between mycobacteria. Scand. J. Immunol., 9: 115-124, 1979.
3. HIGUSHI, S.; MORITAKA, S.; DANNEMBERG Jr., A.M. et al. - Persistence of protein, carbohydrate and wax components of tubercle bacilli in dermal BCG lesions. Amer. Rev. resp. Dis., 123: 396-401, 1981.
4. HUMPHREY, D.M. & WEINER, M.H. - Mycobacterial antigens detection by immunohistochemistry in pulmonary tuberculosis. Hum. Path., 18: 701-708, 1987.
5. MC CUNE, R.M.; FELDMAN, F.M.; LAMBERT, H.P. & MC DERMOTT, W. - Microbial persistence. I. The capacity of tubercle bacilli to survive sterelization in mouse tissues. J. exp. Med., 123: 445-468, 1966.
6. MICHALANY, J. - Técnica histológica em anatomia patológica. Săo Paulo, E. P. U., 1980. p.155.
7. MSHANA, R.N.; BELEHU, A.; STONER, G.L.; HARBOE, M. & HAREGEWOIN, A. - Demonstration of mycobacterial antigens in leprosy tissues. Int. J. Leprosy, 50: 1, 1982.
8. MSHANA, R.N.; HUMBER, D.P.; HARBOE, M. & BELEHU, A. - Demonstration of mycobacterial antigens in nerve biopsies from leprosy patients using PAP immunoenzyme technique. Clin. Immunol. Immunopath., 29: 359-368, 1983.
9. ORREL, J.M.; BRETT, S.J.; IVANYI, J. et al. - Measurement of tissue distribution of immunoperoxidase staining with polyclonal anti-BCG serum in lung granulomata of mice infected with Mycobacterium tuberculosis. J. Path., 164: 41-45, 1991.
10. WILEY, E.L.; MULHOLLAN, T.J.; BECK, B. et al. - Polyclonal antibodies against Bacillus Calmette-Guerin, Mycobacterium duvalli, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques. Amer. J. clin. Path., 94: 307-312, 1990.

Correspondence to:

Profa. Dra. Maria Irma Seixas Duarte

Faculdade de Medicina da USP, Depto. de Patologia

Av. Dr. Arnaldo, 455 - 1º andar sala 26

01246-903 S. Paulo, SP, Brasil

Telephone/FAX: (55)(11) 881-7799

Publication Dates

Publication in this collection
22 Feb 2007
Date of issue
Feb 1995

History

Received
22 May 1994
Accepted
19 Oct 1994

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BARBOLINE, G.; BISETTI, A.; COLIZZI, V. et al. - Immunohistology analysis of mycobacterial antigens by monoclonal antibodies in tuberculosis and mycobacteriosis. Hum. Path., 20: 1078-1083, 1989.

[2] 2. HARBOE, M; MSHANA, R.N.; CLOSS, O.; KRONVALL, G. & AXELSEN, N.H. - Cross-reaction between mycobacteria. Scand. J. Immunol., 9: 115-124, 1979.

[3] 3. HIGUSHI, S.; MORITAKA, S.; DANNEMBERG Jr., A.M. et al. - Persistence of protein, carbohydrate and wax components of tubercle bacilli in dermal BCG lesions. Amer. Rev. resp. Dis., 123: 396-401, 1981.

[4] 4. HUMPHREY, D.M. & WEINER, M.H. - Mycobacterial antigens detection by immunohistochemistry in pulmonary tuberculosis. Hum. Path., 18: 701-708, 1987.

[5] 5. MC CUNE, R.M.; FELDMAN, F.M.; LAMBERT, H.P. & MC DERMOTT, W. - Microbial persistence. I. The capacity of tubercle bacilli to survive sterelization in mouse tissues. J. exp. Med., 123: 445-468, 1966.

[6] 6. MICHALANY, J. - Técnica histológica em anatomia patológica. Săo Paulo, E. P. U., 1980. p.155.

[7] 7. MSHANA, R.N.; BELEHU, A.; STONER, G.L.; HARBOE, M. & HAREGEWOIN, A. - Demonstration of mycobacterial antigens in leprosy tissues. Int. J. Leprosy, 50: 1, 1982.

[8] 8. MSHANA, R.N.; HUMBER, D.P.; HARBOE, M. & BELEHU, A. - Demonstration of mycobacterial antigens in nerve biopsies from leprosy patients using PAP immunoenzyme technique. Clin. Immunol. Immunopath., 29: 359-368, 1983.

[9] 9. ORREL, J.M.; BRETT, S.J.; IVANYI, J. et al. - Measurement of tissue distribution of immunoperoxidase staining with polyclonal anti-BCG serum in lung granulomata of mice infected with Mycobacterium tuberculosis. J. Path., 164: 41-45, 1991.

[10] 10. WILEY, E.L.; MULHOLLAN, T.J.; BECK, B. et al. - Polyclonal antibodies against Bacillus Calmette-Guerin, Mycobacterium duvalli, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques. Amer. J. clin. Path., 94: 307-312, 1990.