Evolution of sarcoma 180 (ascitic tumor) in mice infected with Schistosoma mansoni

Pereira, Fausto Edmundo Lima; Raso, Pedro; Coelho, Paulo Marcos Zech

doi:10.1590/S0037-86821986000100009

Abstracts

Mice infected with 60 cercariae of Schistosoma mansoni were more resistant to the sarcoma 180 ascites tumor. Tumor inoculation was performed 50 days after schistosoma infection and the animals were observed and weighed at 48 hours intervals for development and progression of malignancy. In infected mice the weight gain (ascites formation) started later and was shorter than in uninfected Controls. Also, the number of tumor cells into the peritoneal cavity 72h after tumor implantation was shorter in infected group than incontrols. This in creased resistance against a transplantable tumor probably is related to the effect of endotoxin on tumoricidal activity of macrophages activated by the infection. The immunodepression induced by Schistosoma mansoni infection enhances the proliferation of endogenous bacteria increasing the amount of endotoxin absorbed from the gut.

Experimental Schistosomiasis; Sarcoma 180; Schistosomiasis and cancer

Camundongos infectados com 60 cercárias de Schistosoma mansoni tomaram-se mais resistentes ao sarcoma 180 na forma de tumor ascítico. A inoculação das células tumorais foi feita no 50º dia de infecção e a evolução do tumor foi acompanhada através dapesagem dos animais com intervalos de 48 horas. Nos camundongos infectados o ganho de peso (formação da ascite) começou mais tarde e foi menor do que nos controles não infectados. Também o número de células tumorais na cavidade peritoneal 72 horas após a implantação do tumor foi menor no grupo infectado. Este aumento de resistência a um tumor transplantávelpossivelmente está relacionado ao efeito de endotoxinas sobre a atividade tumoricida dos macrofagos ativados pela infecção. A imunossupressão induzida pela infecção favorece a proliferação de bactérias da flora endógena aumentando a quantidade de endotoxinas absorvidas pelo intestino.

Esquistossomose experimental; Sarcoma 180; Esquistossomose e Câncer

ARTICLES

Evolution of sarcoma 180 (ascitic tumor) in mice infected with Schistosoma mansoni

Fausto Edmundo Lima Pereira^I; Pedro Raso^II; Paulo Marcos Zech Coelho^II

^IDepartamento de Patologia Centro Biomédico UFES. Caixa Postal 780-29000 - Vitória - E. Santo - Brasil

^IIGrupo Integrado de Pesquisa em Esquistossomose ICB- UFMG

^{Adress to correspondence} Endereço para correspondência: Fausto Edmundo Lima Pereira Dept o de Patologia Centro Biomédico da Universidade Federal do Espírito Santo Caixa Postal 780 29000 Vitória - E. Santo. Brasil.

ABSTRACT

Mice infected with 60 cercariae of Schistosoma mansoni were more resistant to the sarcoma 180 ascites tumor. Tumor inoculation was performed 50 days after schistosoma infection and the animals were observed and weighed at 48 hours intervals for development and progression of malignancy. In infected mice the weight gain (ascites formation) started later and was shorter than in uninfected Controls. Also, the number of tumor cells into the peritoneal cavity 72h after tumor implantation was shorter in infected group than incontrols. This in creased resistance against a transplantable tumor probably is related to the effect of endotoxin on tumoricidal activity of macrophages activated by the infection. The immunodepression induced by Schistosoma mansoni infection enhances the proliferation of endogenous bacteria increasing the amount of endotoxin absorbed from the gut.

Keywords: Experimental Schistosomiasis. Sarcoma 180. Schistosomiasis and cancer.

RESUMO

Camundongos infectados com 60 cercárias de Schistosoma mansoni tomaram-se mais resistentes ao sarcoma 180 na forma de tumor ascítico. A inoculação das células tumorais foi feita no 50^o dia de infecção e a evolução do tumor foi acompanhada através dapesagem dos animais com intervalos de 48 horas. Nos camundongos infectados o ganho de peso (formação da ascite) começou mais tarde e foi menor do que nos controles não infectados. Também o número de células tumorais na cavidade peritoneal 72 horas após a implantação do tumor foi menor no grupo infectado. Este aumento de resistência a um tumor transplantávelpossivelmente está relacionado ao efeito de endotoxinas sobre a atividade tumoricida dos macrofagos ativados pela infecção. A imunossupressão induzida pela infecção favorece a proliferação de bactérias da flora endógena aumentando a quantidade de endotoxinas absorvidas pelo intestino.

Palavras-chave: Esquistossomose experimental. Sarcoma 180. Esquistossomose e Câncer.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Recebido para publicação em 19/8/1985.

This work was supported by CNPq and FINEP.

References

1. Andrade Z, Abreu WN. Follicular lymphoma of the spleen in patients with hepatosplenic Schistosomiasis mansoni American Journal of Tropical Medicine and Hygiene 20:237-243, 1971.
2. Araújo FG, Coelho PMZ, Pereira LH, Pellegrino J. Schistosoma mansoni: impairment of cell mediated immune response in mice. Clinical and Experimental Immunology 28:289-291, 1977.
3. Carswell EA, Old LJ, Kassel RI, Fiore N, Williamsom B. An endotoxin induced serum factor will cause necrosis of tumors. Proceedings of the National Academy of Sciences (USA) 72:3666-3670, 1975.
4. Cheever AW. Schistosomiasis and neoplasia. Journal of the National Cancer Institute 61:13-18. 1978.
5. Domingo ED, Warren KS, Stenger RJ. Increased incidence of hepatoma in mice with chronic schistosomiasis treated with a carcinogen. American Journal of Pathology 51:307-321, 1967.
6. Edington GM, von Lichtenberg F, Nawabuebo I, Taylor JR, Smith JN. Pathologic effects of schistosomiasis in Ibadan, Western State of Nigéria. I-Incidence and intensity of infection. Distribution and severity of lesions. American Journal of Tropical Medicine and Hygiene 19:982-985, 1970.
7. Haese WH, Smith DL, Bueding E. Hycanthone induced hepatic changes in mice infected with Schistosoma mansoni Journal of Pharmacology and Experimental Therapheutics 186:430-440, 1973.
8. James SL, Ladins JK, Meltzer MS, Sher A. Macrophages as effector cells of protective immunity in murine schistosomiasis. I-Activation of peritoneal macrophages during natural infection. Cellular Immunology 67:255- 266, 1982.
9. Keller R, Ogilvie BM, Simpson E. Tumor growth in nematode infected animais. Lancet 1:678-680, 1971.
10. Lubiniecki AS, Cypess RH. Quantitative studies of effect of previous Trichinella spiralis infection on sarcoma 180 ascites tumor. Tropenmedizin und Parasitologie 26:329-334, 1976.
11. Meerovitch E, Bomford R. Macrophage potentiation by Trichinella spiralis Annals of Tropical Medicine and Parasitology 71:245-251, 1977.
12. Meltzer MS, Rucco LP, Boraschi D, Nacy CA. Macrophage activation for tumor citotoxicity: analysis or intermediary factors. Journal of Reticuloendothelial Society 26:403-415, 1979.
13. Molinari JA, Garrick L, Lubiniecki AS. Influence of Trichinella spiralis infection on development of sarcoma 180 ascites tumor. Tropenmedizin und Parasitologie 30:439-441, 1979.
14. Mota-Santos TA, Gazzinelli G, Ramalho-Pinto FJ, Pellegrino J, Silva WD. Immunodepression in mice following Schistosoma mansoni infection. Revista do Instituto de Medicina Tropical de São Paulo 18:246- 250, 1976.
15. Mott KE. Possible relationship of Schistosoma infection and liver carcinoma. Transactions of the Royal Society of Tropical Medicine and Hygiene 72:552-553, 1978.
16. North RJ, Kirstein DP, Tutle RL. Subversion of host defense mechanisms by murine tumors. I. A circulating factor that suppresses macrophage mediated resistance to infection. Journal of Experimental Medicine 143:555- 562, 1976.
17. Paes AP, Marigo C. Linfoma folicular gigante do baço e esquistossomose mansônica. Revista do Instituto de Medicina Tropical de São Paulo 23:287-292, 1981.
18. Peley RP, Ruffier JJ, WarrenKS. The suppressive effect of a chronic helminthic infection Schistosomiasis mansoni on the in vitro response of spleen and lymphnode cells to the T cell mitogens PHA and Con A. Infection andlmmunity 13:1176-1183, 1976.
19. Pereira FEL, Gonçalves CS. The association of Schistosoma mansoni infection with hepatocellular carcinoma. Revista da Sociedade Brasileira de Medicina Tropical 17:73-76, 1984.
20. Wing ES, Krahenbuhl JL, Remington JS. Studies of macrophage function during Trichinella spiralis infection in mice. Immunology 36:479-485, 1979.
21. Yarinski A, Drobeck HP, Freele H, Wiland Y, Gumaer Kl. An 18 month study of the parasitologic and tumorigenic effect of hycanthone in Schistosoma mansoni infected and non infected mice. Toxicology and Applied Pharmacology 27:169-182, 1974.

Endereço para correspondência:

Fausto Edmundo Lima Pereira

Dept

^o de Patologia

Centro Biomédico da Universidade Federal do Espírito Santo

Caixa Postal 780

29000

Vitória - E. Santo. Brasil.

Publication Dates

Publication in this collection
06 June 2013
Date of issue
Mar 1986

History

Received
19 Aug 1985
Accepted
19 Aug 1985

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Andrade Z, Abreu WN. Follicular lymphoma of the spleen in patients with hepatosplenic Schistosomiasis mansoni American Journal of Tropical Medicine and Hygiene 20:237-243, 1971.

[2] 2. Araújo FG, Coelho PMZ, Pereira LH, Pellegrino J. Schistosoma mansoni: impairment of cell mediated immune response in mice. Clinical and Experimental Immunology 28:289-291, 1977.

[3] 3. Carswell EA, Old LJ, Kassel RI, Fiore N, Williamsom B. An endotoxin induced serum factor will cause necrosis of tumors. Proceedings of the National Academy of Sciences (USA) 72:3666-3670, 1975.

[4] 4. Cheever AW. Schistosomiasis and neoplasia. Journal of the National Cancer Institute 61:13-18. 1978.

[5] 5. Domingo ED, Warren KS, Stenger RJ. Increased incidence of hepatoma in mice with chronic schistosomiasis treated with a carcinogen. American Journal of Pathology 51:307-321, 1967.

[6] 6. Edington GM, von Lichtenberg F, Nawabuebo I, Taylor JR, Smith JN. Pathologic effects of schistosomiasis in Ibadan, Western State of Nigéria. I-Incidence and intensity of infection. Distribution and severity of lesions. American Journal of Tropical Medicine and Hygiene 19:982-985, 1970.

[7] 7. Haese WH, Smith DL, Bueding E. Hycanthone induced hepatic changes in mice infected with Schistosoma mansoni Journal of Pharmacology and Experimental Therapheutics 186:430-440, 1973.

[8] 8. James SL, Ladins JK, Meltzer MS, Sher A. Macrophages as effector cells of protective immunity in murine schistosomiasis. I-Activation of peritoneal macrophages during natural infection. Cellular Immunology 67:255- 266, 1982.

[9] 9. Keller R, Ogilvie BM, Simpson E. Tumor growth in nematode infected animais. Lancet 1:678-680, 1971.

[10] 10. Lubiniecki AS, Cypess RH. Quantitative studies of effect of previous Trichinella spiralis infection on sarcoma 180 ascites tumor. Tropenmedizin und Parasitologie 26:329-334, 1976.

[11] 11. Meerovitch E, Bomford R. Macrophage potentiation by Trichinella spiralis Annals of Tropical Medicine and Parasitology 71:245-251, 1977.

[12] 12. Meltzer MS, Rucco LP, Boraschi D, Nacy CA. Macrophage activation for tumor citotoxicity: analysis or intermediary factors. Journal of Reticuloendothelial Society 26:403-415, 1979.

[13] 13. Molinari JA, Garrick L, Lubiniecki AS. Influence of Trichinella spiralis infection on development of sarcoma 180 ascites tumor. Tropenmedizin und Parasitologie 30:439-441, 1979.

[14] 14. Mota-Santos TA, Gazzinelli G, Ramalho-Pinto FJ, Pellegrino J, Silva WD. Immunodepression in mice following Schistosoma mansoni infection. Revista do Instituto de Medicina Tropical de São Paulo 18:246- 250, 1976.

[15] 15. Mott KE. Possible relationship of Schistosoma infection and liver carcinoma. Transactions of the Royal Society of Tropical Medicine and Hygiene 72:552-553, 1978.

[16] 16. North RJ, Kirstein DP, Tutle RL. Subversion of host defense mechanisms by murine tumors. I. A circulating factor that suppresses macrophage mediated resistance to infection. Journal of Experimental Medicine 143:555- 562, 1976.

[17] 17. Paes AP, Marigo C. Linfoma folicular gigante do baço e esquistossomose mansônica. Revista do Instituto de Medicina Tropical de São Paulo 23:287-292, 1981.

[18] 18. Peley RP, Ruffier JJ, WarrenKS. The suppressive effect of a chronic helminthic infection Schistosomiasis mansoni on the in vitro response of spleen and lymphnode cells to the T cell mitogens PHA and Con A. Infection andlmmunity 13:1176-1183, 1976.

[19] 19. Pereira FEL, Gonçalves CS. The association of Schistosoma mansoni infection with hepatocellular carcinoma. Revista da Sociedade Brasileira de Medicina Tropical 17:73-76, 1984.

[20] 20. Wing ES, Krahenbuhl JL, Remington JS. Studies of macrophage function during Trichinella spiralis infection in mice. Immunology 36:479-485, 1979.

[21] 21. Yarinski A, Drobeck HP, Freele H, Wiland Y, Gumaer Kl. An 18 month study of the parasitologic and tumorigenic effect of hycanthone in Schistosoma mansoni infected and non infected mice. Toxicology and Applied Pharmacology 27:169-182, 1974.