Histopatologia da esquistossomose mansoni em fígado de Mus musculus infectado por amostras humanas de fase aguda e crônica da periferia de Belo Horizonte, Minas Gerais

Carvalho, Amélia Dulce Vilela de; Alvarenga, Roberto Junqueira de; Melo, Alan Lane de

doi:10.1590/S0037-86821986000200007

Resumos

Exemplares de Biomphalaria glabrataforam infectados com miracídios obtidos de ovos de Schistosoma mansoni, encontrados em fezes de indivíduos de 7 a 18 anos, da região de Lagoa da Pampulha, Belo Horizonte, MG. Os pacientes de fase aguda se infectaram em uma primeira visita ao foco. Os da fase crônica eram moradores próximos aos focos. Para cada caso clínico, isolou-se a respectiva amostra do parasita. Foram infectados pela cauda 55 camundongos fêmeas com 70 ± 10 cercárias. Cortes histológicos de fígados, corados por HE, tricrômico de Gomori, impregnação metálica pela prata, e PAS foram observados à microscopia óptica. Não houve diferenças estatísticas em relação às médias das mensurações dos diâmetros dos granulomas referentes às amostras e datas de sacrifícios. Os granulomas apresentaram fase exsudativa do tipo Ha (reação de inflamação mista) e IIIa (granuloma com células epitelióides). Com amostras de pacientes em fase aguda o padrão predominante foi a Ha na 7ª semana. Na 10.ª semana predominaram granulomas do tipo IIIa. Nas amostras de pacientes em fase crônica, verificou-se uma mescla de granulomas do tipo Ha e IIIa na 7ª semana. Na 10ª semana predominou o tipo IIIa. Alguns aspectos histopatológicos de fígados foram descritos e comparados com aqueles existentes na literatura.

Schistosoma mansoni; Histopatologia; Granuloma

Specimens o/Biomphalaria glabrata were infected with miracidia obtained from eggs of Schistosoma mansoni found in the feces of patients aged 7 to 18 years old, from Lagoa da Pampulha area, Belo Horizonte, Minas Gerais, tírazil. The patients in the acnte phase were infected on their first visit to the focus. The patients with chronic phase disease were living around the focus. Egg samples were isolate from each clinical case. Fifty five female miee were infected by their tails with 70 ± 10 cercaria Seclions of lirer were stained bv HE, Gomori trichrome, silver impregnation, and PAS, and examined by optical microscopy. No statistical difference was observed between the average diameter of the granulomas and the sample and date of sacrifice of the mice (7th and 10th weeks). The granulomas were in the exudative phase; type IIa (mixed inflammatory reactions) and type IIIa (granulomas with epithelioid cells). At the 7th week samples of the patients in acute phase showed granulomas type IIa. Type IIIa were predominam at the 10th' week. At the 7th week, in samples from chronic phase patients, a mixture of type lia and IIIa granulomas was observed. Some histopathologic aspects of the liver are described and compared with those already found in the literature.

Schistosoma mansoni; Histopathology; Granuloma

ARTIGOS

Histopatologia da esquistossomose mansoni em fígado de Mus musculus infectado por amostras humanas de fase aguda e crônica da periferia de Belo Horizonte, Minas Gerais

Amélia Dulce Vilela de Carvalho^I; Roberto Junqueira de Alvarenga^II; Alan Lane de Melo^III

^IDepartamento de Morfologia do Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

^IIDepartamento de Patologia Geral, ICB/UFMG

^IIIDepartamento de Parasitologia, ICB/UFMG

^{Endereço para correspondência} Endereço para correspondência: Dra. Amélia Dulce Vilela de Carvalho Rua Santiago 118, ap. 302 Sion 30310 Belo Horizonte, MG, Brasil.

RESUMO

Exemplares de Biomphalaria glabrataforam infectados com miracídios obtidos de ovos de Schistosoma mansoni, encontrados em fezes de indivíduos de 7 a 18 anos, da região de Lagoa da Pampulha, Belo Horizonte, MG. Os pacientes de fase aguda se infectaram em uma primeira visita ao foco. Os da fase crônica eram moradores próximos aos focos. Para cada caso clínico, isolou-se a respectiva amostra do parasita. Foram infectados pela cauda 55 camundongos fêmeas com 70 ± 10 cercárias. Cortes histológicos de fígados, corados por HE, tricrômico de Gomori, impregnação metálica pela prata, e PAS foram observados à microscopia óptica. Não houve diferenças estatísticas em relação às médias das mensurações dos diâmetros dos granulomas referentes às amostras e datas de sacrifícios. Os granulomas apresentaram fase exsudativa do tipo Ha (reação de inflamação mista) e IIIa (granuloma com células epitelióides). Com amostras de pacientes em fase aguda o padrão predominante foi a Ha na 7^a semana. Na 10.^a semana predominaram granulomas do tipo IIIa. Nas amostras de pacientes em fase crônica, verificou-se uma mescla de granulomas do tipo Ha e IIIa na 7^a semana. Na 10^a semana predominou o tipo IIIa. Alguns aspectos histopatológicos de fígados foram descritos e comparados com aqueles existentes na literatura.

Palavras-chave:Schistosoma mansoni. Histopatologia. Granuloma.

ABSTRACT

Specimens o/Biomphalaria glabrata were infected with miracidia obtained from eggs of Schistosoma mansoni found in the feces of patients aged 7 to 18 years old, from Lagoa da Pampulha area, Belo Horizonte, Minas Gerais, tírazil. The patients in the acnte phase were infected on their first visit to the focus. The patients with chronic phase disease were living around the focus. Egg samples were isolate from each clinical case. Fifty five female miee were infected by their tails with 70 ± 10 cercaria Seclions of lirer were stained bv HE, Gomori trichrome, silver impregnation, and PAS, and examined by optical microscopy. No statistical difference was observed between the average diameter of the granulomas and the sample and date of sacrifice of the mice (7th and 10th weeks). The granulomas were in the exudative phase; type IIa (mixed inflammatory reactions) and type IIIa (granulomas with epithelioid cells). At the 7^th week samples of the patients in acute phase showed granulomas type IIa. Type IIIa were predominam at the 10^th' week. At the 7^th week, in samples from chronic phase patients, a mixture of type lia and IIIa granulomas was observed.

Some histopathologic aspects of the liver are described and compared with those already found in the literature.

Keywords:Schistosoma mansoni. Histopathology. Granuloma.

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Recebido para publicação em 18/10/84.

Trabalho realizado com o auxilio do Conselho Nacional de Desenvolvimento Cientifico e Tecnológico - CNPq e Universidade Federal de Minas Gerais, Brasil.

1. Andrade ZA. Patologia geral da esquistossomose. In: de Paola D. (ed.). Mecanismo Básico de Doença. Livraria Athcneu. Rio de Janeiro, p. 134-137. 1977.
2. Bhattacharyya KK. Low protein diet as a factor in the production of experimental schistosomiasis hepatic fibrosis. Journal of Pathology and Bacteriology 89: 1 3-21, 1965.
3. Cheever AW. A comparative study of Schistosomiasis mansoni in mice. gcrbils, multimammate rats and hamsters. 1. The rclation of portal hypertension tosize of hepatic granulomas. The American Journal of Tropical Medicine and Hygiene 14:21 1-226. 1965.
4. Cheever AW. De Witt WB. Warren KS. Repeated infection and treatment of mice with Schistosoma mansoni: functional. anatomic and immunological observations. American Journal of Tropical Medicine and Hygiene 14:239-253. 1965.
5. Colley DG. Savage AM. Lewis FA. Host responses induced and elicited by cercariae. schistosomula and cercaria! antigenic preparations. The American Journal of Tropical Medicine and Hygiene 26:88-95. 1977.
6. de Paola D. Mecanismo Básico de Doença. Livraria Athcneu. Rio de Janeiro. 134-137, 1977.
7. Dias RP. Alvarenga RJ. Indução experimental da fibrose de Symmers em camundongo. Revista do Instituto de Medicina Tropical de São Paulo 15: 60-65, 1973.
8. Domingos EO. Warren KS. Endogenous desensitization changing host granulomatous response todifferentstagcs of infection with S. mansoni American Journal of Pathology 52: 369-377. 1968.
9. Erickson DG. Jones CE. Tang DB. Schistosomiasis mansoni hacmatobia and japonica in hamster: liver granuloma measurements. Experimental Parasitology 35: 425-433. 1974.
10. Grove Dl. Warren KS. Rclation of intensitv of infection to diseasc in hamsters with acute Schistosomiasis mansoni American Journal of Tropical Medicine and Hygiene 25: 608-612, 1976.
11. Hsu H, Li Hsu, 1958 in Warren KS. A comparison of Puerto Rican, Brazilian, Egyptian, and Tanzanian strains of Schistosoma mansoni in mice: penetration of cercariae, maturation of schistosomes and production of liver disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 61: 795-801, 1967.
12. Hsu Li, Hsu FS, Lust GL, Davis Jr, Cleveland LK. Comparative studies on the lesions caused by eggs of Schistosoma japonicum and mansoni in the liver of hamster, guinea pig and albino rats. Annals of Tropical Medicine and Parasitology 67: 349-356, 1973.
13. Magzoub M, Adam SEI. Experimental infection and hepatic changes in mice infected with Schistosoma mansoni (Sudan strain). Journal of Pathology 113: 47- 52, 1974.
14. Motta JC. II Simpósio sobre Esquistossomose. In Prata A, e Calbin e (Ed.) Faculdade de Medicina da Universidade Federal da Bahia, Brasil, 1970.
15. Mousa AH, El Garem A, El Raziki EH, El Abdel-Latif A, Selim A. Wassef SA, Habib A, Shenawy A. Prcliminary studies on prophylaxis, drug resistance and synergism in experimental schistosomiasis. Annals of the New York Acadcmy of Sciences 160: 461-485, 1969.
16. Prata A, Aboim E. 2.° Simpósio sobre Esquistossomose, Salvador, Bahia. Ed. Faculdade de Medicina da Universidade Federal da Bahia, Brasil, 1970.
17. Raso P, Bernardes RC, Tafuri WL, Bogliolo L, Neves J. As dimensões do granuloma causado pelos ovos de Schistosoma mansoni no fígado humano. Revista da Sociedade Brasileira de Medicina Tropical 12: 45-49, 1978.
18. Saoud MFA. Comparative study on the characteristics of some gcographical strains of Schistosoma mansoni in mice and hamster. Journal of Helminthology 39: 111- 112. 1965.
19. von Lichtenberg F, Erickson DG, Sadun EH. Com-parative histopathology of schistosome granulomas in the hamster. American Journal of Pathology 72: 140-178. 1973.
20. Warren KS. The pathogenesis of "clay-pipe stem cirrhosis" in mice with chronic Schistosomiasis mansoni with a note on the longevity of the schistosomes. American Journal of Pathology 49:477-485, 1966.
21. Warren KS. A comparison of Puerto Rican, Brazilian. Hgyptian. and Tanzanian strains of Schistosoma mansoni in mice: penetration of cercariae. maturation of schistosomes and production of liver disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 61: 795-801. 1967.
22. Warren KS. Berry EG. Induction of hepatosplenic discasc by single pairs of the Philippine. Formosan. Japanese. and Chinese strains of Schistosoma japonicum Journal of Inlcctious Discases 126: 482-491, 1972.

Endereço para correspondência:

Dra. Amélia Dulce Vilela de Carvalho

Rua Santiago 118, ap. 302

Sion

30310

Belo Horizonte, MG, Brasil.

Datas de Publicação

Publicação nesta coleção
06 Jun 2013
Data do Fascículo
Jun 1986

Histórico

Recebido
18 Out 1984
Aceito
18 Out 1984

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Andrade ZA. Patologia geral da esquistossomose. In: de Paola D. (ed.). Mecanismo Básico de Doença. Livraria Athcneu. Rio de Janeiro, p. 134-137. 1977.

[2] 2. Bhattacharyya KK. Low protein diet as a factor in the production of experimental schistosomiasis hepatic fibrosis. Journal of Pathology and Bacteriology 89: 1 3-21, 1965.

[3] 3. Cheever AW. A comparative study of Schistosomiasis mansoni in mice. gcrbils, multimammate rats and hamsters. 1. The rclation of portal hypertension tosize of hepatic granulomas. The American Journal of Tropical Medicine and Hygiene 14:21 1-226. 1965.

[4] 4. Cheever AW. De Witt WB. Warren KS. Repeated infection and treatment of mice with Schistosoma mansoni: functional. anatomic and immunological observations. American Journal of Tropical Medicine and Hygiene 14:239-253. 1965.

[5] 5. Colley DG. Savage AM. Lewis FA. Host responses induced and elicited by cercariae. schistosomula and cercaria! antigenic preparations. The American Journal of Tropical Medicine and Hygiene 26:88-95. 1977.

[6] 6. de Paola D. Mecanismo Básico de Doença. Livraria Athcneu. Rio de Janeiro. 134-137, 1977.

[7] 7. Dias RP. Alvarenga RJ. Indução experimental da fibrose de Symmers em camundongo. Revista do Instituto de Medicina Tropical de São Paulo 15: 60-65, 1973.

[8] 8. Domingos EO. Warren KS. Endogenous desensitization changing host granulomatous response todifferentstagcs of infection with S. mansoni American Journal of Pathology 52: 369-377. 1968.

[9] 9. Erickson DG. Jones CE. Tang DB. Schistosomiasis mansoni hacmatobia and japonica in hamster: liver granuloma measurements. Experimental Parasitology 35: 425-433. 1974.

[10] 10. Grove Dl. Warren KS. Rclation of intensitv of infection to diseasc in hamsters with acute Schistosomiasis mansoni American Journal of Tropical Medicine and Hygiene 25: 608-612, 1976.

[11] 11. Hsu H, Li Hsu, 1958 in Warren KS. A comparison of Puerto Rican, Brazilian, Egyptian, and Tanzanian strains of Schistosoma mansoni in mice: penetration of cercariae, maturation of schistosomes and production of liver disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 61: 795-801, 1967.

[12] 12. Hsu Li, Hsu FS, Lust GL, Davis Jr, Cleveland LK. Comparative studies on the lesions caused by eggs of Schistosoma japonicum and mansoni in the liver of hamster, guinea pig and albino rats. Annals of Tropical Medicine and Parasitology 67: 349-356, 1973.

[13] 13. Magzoub M, Adam SEI. Experimental infection and hepatic changes in mice infected with Schistosoma mansoni (Sudan strain). Journal of Pathology 113: 47- 52, 1974.

[14] 14. Motta JC. II Simpósio sobre Esquistossomose. In Prata A, e Calbin e (Ed.) Faculdade de Medicina da Universidade Federal da Bahia, Brasil, 1970.

[15] 15. Mousa AH, El Garem A, El Raziki EH, El Abdel-Latif A, Selim A. Wassef SA, Habib A, Shenawy A. Prcliminary studies on prophylaxis, drug resistance and synergism in experimental schistosomiasis. Annals of the New York Acadcmy of Sciences 160: 461-485, 1969.

[16] 16. Prata A, Aboim E. 2.° Simpósio sobre Esquistossomose, Salvador, Bahia. Ed. Faculdade de Medicina da Universidade Federal da Bahia, Brasil, 1970.

[17] 17. Raso P, Bernardes RC, Tafuri WL, Bogliolo L, Neves J. As dimensões do granuloma causado pelos ovos de Schistosoma mansoni no fígado humano. Revista da Sociedade Brasileira de Medicina Tropical 12: 45-49, 1978.

[18] 18. Saoud MFA. Comparative study on the characteristics of some gcographical strains of Schistosoma mansoni in mice and hamster. Journal of Helminthology 39: 111- 112. 1965.

[19] 19. von Lichtenberg F, Erickson DG, Sadun EH. Com-parative histopathology of schistosome granulomas in the hamster. American Journal of Pathology 72: 140-178. 1973.

[20] 20. Warren KS. The pathogenesis of "clay-pipe stem cirrhosis" in mice with chronic Schistosomiasis mansoni with a note on the longevity of the schistosomes. American Journal of Pathology 49:477-485, 1966.

[21] 21. Warren KS. A comparison of Puerto Rican, Brazilian. Hgyptian. and Tanzanian strains of Schistosoma mansoni in mice: penetration of cercariae. maturation of schistosomes and production of liver disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 61: 795-801. 1967.

[22] 22. Warren KS. Berry EG. Induction of hepatosplenic discasc by single pairs of the Philippine. Formosan. Japanese. and Chinese strains of Schistosoma japonicum Journal of Inlcctious Discases 126: 482-491, 1972.