Ocorrência de rota vírus e adeno vírus em crianças de até 11 anos de idade sem sintomatologia de diarréia em Goiânia - GO

Camarota, Sandra Cristina T.; Azevedo, Marli da Silva P. de; Martins, Regina M. B.; Barbosa, Aristides J.; Ferreira Júnior, Pedro A.; Araújo, Sérgio V. de; Cardoso, Divina das Dores de Paula

doi:10.1590/S0037-86821992000100005

Resumos

Trezentas e oitenta e cinco amostras fecais provenientes de crianças na faixa etária de até 11 anos, sem sintomatologia de diarréia, foram estudadas objetivando-se a detecção de rotavirus. Desta amostragem, 268foram obtidas de crianças habitantes de creches e 117 de crianças atendidas no ambulatório do Hospital Lúcio Rebelo de Goiânia-Goiás. Todas as amostrasforam analisadas através da técnica de eletroforese em gel de poliacrilamida (EGPA- SDS), e 89 foram também analisadas pelo ensaio imunoenzimático adaptado para rotavirus e adenovirus (E1ARA). Rotavirus e adenovirus só foram detectados nas crianças atendidas no ambulatório, num percentual de 1,7% e 1,6% respectivamente, não havendo nenhuma positividade nas crianças de creches. Ambos os vírus ocorrerarh na faixa etária de 1 a 2 anos.

Rotavirus; Adenovirus; Excreção assintomática; Creches

Three hundred and eigthy five children without a diagnosis of diarrhoeal illness had faecal specimens collected in order to detect rotavirus infection. The survey was conducted in Goiânia city - CentralBrazil and the children aged 0-11 years old were recruited (268 in day care centers and 117 from a local outpatient clinic - Hospital Lucio Rebelo). Detection of rotavirus was done by Polyacrylamide gel electrophoresis (PAGE - SDS), and 89 specimens were also analyzed by commercial enzyme immunoassays for rotavirus and adenovirus (EIARA). Rotaviruses and adenoviruses were only detected among the children attending the outpatient clinic. Prevalence rates of rotavirus and adenovirus excretion were of 1.7% and 1.6%, respectively. Both viruses were found among children from 1 to 2 years old.

Rotavirus; Adenovirus; Asymptomatic excretion; Day care centers

ARTIGOS

Ocorrência de rota vírus e adeno vírus em crianças de até 11 anos de idade sem sintomatologia de diarréia em Goiânia - GO

Sandra Cristina T. Camarota; Marli da Silva P. de Azevedo; Regina M. B. Martins; Aristides J. Barbosa; Pedro A. Ferreira Júnior; Sérgio V. de Araújo; Divina das Dores de Paula Cardoso

^{Endereço para correspondência} Endereço para correspondência: Profa. Divina das Dores P. Cardoso. Depto. de Microbiologia/IPTESP/UFG. Rua Delenda Rezende de Melo esq/com 1 a Avenida S. Universitário Caixa Postal 131 74230-970 Goiânia-GO.

RESUMO

Trezentas e oitenta e cinco amostras fecais provenientes de crianças na faixa etária de até 11 anos, sem sintomatologia de diarréia, foram estudadas objetivando-se a detecção de rotavirus. Desta amostragem, 268foram obtidas de crianças habitantes de creches e 117 de crianças atendidas no ambulatório do Hospital Lúcio Rebelo de Goiânia-Goiás. Todas as amostrasforam analisadas através da técnica de eletroforese em gel de poliacrilamida (EGPA- SDS), e 89 foram também analisadas pelo ensaio imunoenzimático adaptado para rotavirus e adenovirus (E1ARA). Rotavirus e adenovirus só foram detectados nas crianças atendidas no ambulatório, num percentual de 1,7% e 1,6% respectivamente, não havendo nenhuma positividade nas crianças de creches. Ambos os vírus ocorrerarh na faixa etária de 1 a 2 anos.

Palavras-chave: Rotavirus. Adenovirus. Excreção assintomática. Creches.

ABSTRACT

Three hundred and eigthy five children without a diagnosis of diarrhoeal illness had faecal specimens collected in order to detect rotavirus infection. The survey was conducted in Goiânia city - CentralBrazil and the children aged 0-11 years old were recruited (268 in day care centers and 117 from a local outpatient clinic - Hospital Lucio Rebelo). Detection of rotavirus was done by Polyacrylamide gel electrophoresis (PAGE - SDS), and 89 specimens were also analyzed by commercial enzyme immunoassays for rotavirus and adenovirus (EIARA). Rotaviruses and adenoviruses were only detected among the children attending the outpatient clinic. Prevalence rates of rotavirus and adenovirus excretion were of 1.7% and 1.6%, respectively. Both viruses were found among children from 1 to 2 years old.

Keywords: Rotavirus. Adenovirus. Asymptomatic excretion. Day care centers.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido para publicação em 11/04/91.

Laboratório de Virologia, Departamento de Microbiologia do Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia-GO.

1. Albert MJ, Unicomb LE, Barnes GL, Bishop RF. Cultivation and characterization of rotavirus strains infecting newborn babies in Melbourne, Austrália, from 1975 to 1979. Journal of Clinical Microbiology 25:1635-1640, 1987.
2. Al-Frayh AR, Ramia S, Bakir TMF, Zaidi A. Rotavirus shedding by neonates and possible modes of transmission. Journal of Tropical Pediatrics 33:246-248, 1987.
3. Bishop RF, Davidson GP, Holmes UH, Ruck BJ. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. The Lancet 1:1281-1283, 1973.
4. Black RE, Brown KH, Becker S, Alin ARM A. Longitudinal studies of infectious diseases and physical growth of children in riiral Bangladesh II. Incidence of diarrhea and association with known pathogens. American Journal of Epidemiology 115:315-324, 1982.
5. Brandt CD, Kim HW, Yolken RH, Kapikian AZ, Arrobio JO, Rodrigues WJ, Wyatt RG, Chanock RM, Parrott RH. Comparative epidemiology with pediatric gastroenteritis. American Journal of Epidemiology 110:243-254, 1979.
6. Cardoso DDP, Brito WMED, Martins RMB, Kitajima EW, Souza MPM, Barbosa AJ, Oliveira SA, Rascopi SB. Ocorrência de rotavirus e adenovirus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia. Revista da Sociedade Brasileira de Medicina Tropical 22:67- 71, 1989.
7. Champsaur H, Henry-Amar M, Goldszmidt D, Bourjouane M, Bach C. Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. II. Serological response. The Journal of Infectious Diseases 149:675-682, 1984.
8. Champsaur H, Questiaux E, Prevot J, Henry-Amar M, Goldszmidt D, Bouijouane M, Bach C. Rotavirus carriage, asymptomatic infection and disease in the first two years oflife. I. Virus shedding. The Journal of Infectious Diseases 149:667-674, 1984.
9. Cravioto A, Ryes RE, Ortega R, Fernandez G, Hernandez R, Lopes D. Prospective study of diarrhoeal disease in a cohort of rural Mexican- children: incidence and isolated pathogens during the first two years oflife. Epidemiology and Infection 101:123-134, 1988.
10. Echeverria P, Taylor DN, Lexsomboon U, Bhaibulaya M, Blacklow NR, Tamura K, Sakazaki R. Case-control study of endemic diarrheal disease in thai children. The Journal of Infectious Diseases 159:543-548, 1989.
11. Fang ZY, Glass RI, Penaranda M, Dong H, Monroe SS, Wen L, Estes MK, Eiden J, Yolken RH, Saif L, Gouvea V, HungT. Purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins. Journal of Virology 63:2191- 2197, 1989.
12. Flewett TH. Rotavirus vaccines - achievements and prospects. Archives of Disease in Childhood 61:211- 212, 1986.
13. Georges-Courbot MC, Monges J, Berava-Cassel AM, Govandjika I, Georges AJ. Prospective longitudinal study of rotavirus infections in children from birth to two years of age in Central-África. Annais of Institute Pasteur/Virology 139:421-428, 1988.
14. Grimwood K, Abbott GD, Fergusson DM,Jennings LG, Allan JM. Spread of rotavirus within families: a community based study. British Medical Journal 287:575-577, 1983.
15. Gurwith M, Wenman W, Hinde D, Feltheman S, Greenberg H. A prospective study of rotavirus infection in infants and young children. The Journal of Infectious Diseases 144:218-224, 1981.
16. Ho M-S, Glass RI, Pinsky PF, Anderson LJ. Rotavirus as a cause of diarrheal morbidity and mortality in the United States. Journal of Infectious Diseases 158:1112-1116, 1988.
17. Ho M-S, Glass RI, Pinsky PF, Young-Okoh N, Sappenfield WM, Buehier JM, Gunter N, Anderson LJ. Diarrheal deaths in American children. Are they preventable? The Journal of the American Medical Association 260:3281-3285, 1988.
18. Hrdy DB. Epidemiology of rotaviral infection in adults. Reviews of Infectious Diseases 9:461-469, 1987.
19. Ishak R, Linhares AC, Gabbay YB, Ishak MOG, Cardoso DDP. Soroepidemiologia de rotavirus em utna população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26:280-284, 1984.
20. Kapikian AZ, Flores J, Hoshino Y, Glass RI, Midthun K, Gorziglia M, Chanock RM. Rotavirus the major etiologic agent of severe infantile diarrhea may be controlable by a "Jennerian"approach to vaccination. The Journal of Infectious Diseases 153:815-822, 1986.
21. Kapikian AZ, Flores J, Hoshino Y, Midthun K, Gorziglia M, Green KY, Chanock RM, Potash L, Sears SD, Clements ML, Haisey NA, Black RE, Peresschael I. Prospects for development of a rotavirus vaccine against rotavirus diarrhea in infants and young children. Reviews of Infectious Diseases 4:539-546, 1989.
22. KeswickBH, PickeringLK, DupontHL, Woodward WE. Prevalence of rotavirus in children in day care centers. Journal of Pediatrics 103:85-86, 1983.
23. Koopman JS, Monto AS. The tecumseh study XV: Rotavirus infection and pathogenicity. American Journal of Epidemiology 130:750-759, 1989.
24. Koopman JS, Monto AS, Longin IMJR. The tecumseh study XVI: Family and community sources of rotavirus infection. American Journal of Epidemiology 130:760-768, 1989.
25. LaemmliVK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-686, 1970.
26. Leite JPG, Pereira HG, Azevedo RS, Schatzmayr HG. Adenovirus in faeces of children with acute gastroenterite in Rio de Janeiro, Brazil. Journal of Medical Virology 15:203-207, 1985.
27. Louren< jo MH, Nicolas JC, Cohen J, Scherrer R, Bricout F. Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolate in France. Annals of Institute Pauster/ Virology 132:161-173, 1981.
28. Pereira HG, Azevedo RS, Leite JPG, Andrade AP, Castro LA, Combined enzyme immunoassay for rotavirus and adenovirus (EIARA). Journal of Virological Methods 10:21-28, 1985.
29. Pereira HG, Azevedo RS, Leite JPG, Barth OM, Sutmoller JF, Farias V, Vidal MMP. Comparison of polyacrylamid gel electrophoresis (PAGE), immuno- electron microscopy (IEM) and enzyme immunoassay (EIA) for the rapid diagnosis of rotavirus infection in children. Memórias do Instituto Oswaldo Cruz 78:483-490, 1983.
30. Pickering LK, Bartiett AV III, Reves RR, Morrow A. Asymptomatic excretion of rotavirus before and after rotavirus diarrhea in children in day care centers. Journal of Pediatrics 112:261-365, 1988.
31. Rácz ML, Candeias JAN, Trablusi JR, MurahowskiJ. Diarrheal disease in Brazil: Clinical features of rotavirus-associated gastroenteritis in children. European Journal of Epidemiology 4:382- 385, 1988.
32. Raphael RA, Sattar SA, Springthorpe VS. Longterm survival of human rotavirus in rawtreated river water. Canadian Journal Microbiology 31:124-128, 1985.
33. Rodrigues WJ, Kim HW, Brandt CD, Gardner MK, Parrott RH. Use of electrophoresis of RNA from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. The Journal of Infectious Diseases 148:34-40, 1983.
34. Sattar SA, Ijas MK, Johson-Lussenburg CM, Springthorpe VS. Effect of relative humidity on the airborne survival of rotavirus SA-11. Apllied and Environmental Microbiology 47:879-881, 1984.
35. Tiemessen CT, Wegerhoff FO, Erasmus MJ, Kidd AH. Infection by enteric adenovirus, rotavirus and other agents in a rural african environment. Journal of Medical Virology 28:176-182, 1989.

Endereço para correspondência:

Profa. Divina das Dores P. Cardoso.

Depto. de Microbiologia/IPTESP/UFG.

Rua Delenda Rezende de Melo esq/com 1

^a Avenida

S. Universitário

Caixa Postal 131

74230-970

Goiânia-GO.

Datas de Publicação

Publicação nesta coleção
23 Abr 2013
Data do Fascículo
Mar 1992

Histórico

Aceito
11 Abr 1991
Recebido
11 Abr 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Albert MJ, Unicomb LE, Barnes GL, Bishop RF. Cultivation and characterization of rotavirus strains infecting newborn babies in Melbourne, Austrália, from 1975 to 1979. Journal of Clinical Microbiology 25:1635-1640, 1987.

[2] 2. Al-Frayh AR, Ramia S, Bakir TMF, Zaidi A. Rotavirus shedding by neonates and possible modes of transmission. Journal of Tropical Pediatrics 33:246-248, 1987.

[3] 3. Bishop RF, Davidson GP, Holmes UH, Ruck BJ. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. The Lancet 1:1281-1283, 1973.

[4] 4. Black RE, Brown KH, Becker S, Alin ARM A. Longitudinal studies of infectious diseases and physical growth of children in riiral Bangladesh II. Incidence of diarrhea and association with known pathogens. American Journal of Epidemiology 115:315-324, 1982.

[5] 5. Brandt CD, Kim HW, Yolken RH, Kapikian AZ, Arrobio JO, Rodrigues WJ, Wyatt RG, Chanock RM, Parrott RH. Comparative epidemiology with pediatric gastroenteritis. American Journal of Epidemiology 110:243-254, 1979.

[6] 6. Cardoso DDP, Brito WMED, Martins RMB, Kitajima EW, Souza MPM, Barbosa AJ, Oliveira SA, Rascopi SB. Ocorrência de rotavirus e adenovirus em amostras fecais de crianças com gastrenterite, na cidade de Goiânia. Revista da Sociedade Brasileira de Medicina Tropical 22:67- 71, 1989.

[7] 7. Champsaur H, Henry-Amar M, Goldszmidt D, Bourjouane M, Bach C. Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. II. Serological response. The Journal of Infectious Diseases 149:675-682, 1984.

[8] 8. Champsaur H, Questiaux E, Prevot J, Henry-Amar M, Goldszmidt D, Bouijouane M, Bach C. Rotavirus carriage, asymptomatic infection and disease in the first two years oflife. I. Virus shedding. The Journal of Infectious Diseases 149:667-674, 1984.

[9] 9. Cravioto A, Ryes RE, Ortega R, Fernandez G, Hernandez R, Lopes D. Prospective study of diarrhoeal disease in a cohort of rural Mexican- children: incidence and isolated pathogens during the first two years oflife. Epidemiology and Infection 101:123-134, 1988.

[10] 10. Echeverria P, Taylor DN, Lexsomboon U, Bhaibulaya M, Blacklow NR, Tamura K, Sakazaki R. Case-control study of endemic diarrheal disease in thai children. The Journal of Infectious Diseases 159:543-548, 1989.

[11] 11. Fang ZY, Glass RI, Penaranda M, Dong H, Monroe SS, Wen L, Estes MK, Eiden J, Yolken RH, Saif L, Gouvea V, HungT. Purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins. Journal of Virology 63:2191- 2197, 1989.

[12] 12. Flewett TH. Rotavirus vaccines - achievements and prospects. Archives of Disease in Childhood 61:211- 212, 1986.

[13] 13. Georges-Courbot MC, Monges J, Berava-Cassel AM, Govandjika I, Georges AJ. Prospective longitudinal study of rotavirus infections in children from birth to two years of age in Central-África. Annais of Institute Pasteur/Virology 139:421-428, 1988.

[14] 14. Grimwood K, Abbott GD, Fergusson DM,Jennings LG, Allan JM. Spread of rotavirus within families: a community based study. British Medical Journal 287:575-577, 1983.

[15] 15. Gurwith M, Wenman W, Hinde D, Feltheman S, Greenberg H. A prospective study of rotavirus infection in infants and young children. The Journal of Infectious Diseases 144:218-224, 1981.

[16] 16. Ho M-S, Glass RI, Pinsky PF, Anderson LJ. Rotavirus as a cause of diarrheal morbidity and mortality in the United States. Journal of Infectious Diseases 158:1112-1116, 1988.

[17] 17. Ho M-S, Glass RI, Pinsky PF, Young-Okoh N, Sappenfield WM, Buehier JM, Gunter N, Anderson LJ. Diarrheal deaths in American children. Are they preventable? The Journal of the American Medical Association 260:3281-3285, 1988.

[18] 18. Hrdy DB. Epidemiology of rotaviral infection in adults. Reviews of Infectious Diseases 9:461-469, 1987.

[19] 19. Ishak R, Linhares AC, Gabbay YB, Ishak MOG, Cardoso DDP. Soroepidemiologia de rotavirus em utna população infantil, Goiânia, Goiás, Brasil. Revista do Instituto de Medicina Tropical de São Paulo 26:280-284, 1984.

[20] 20. Kapikian AZ, Flores J, Hoshino Y, Glass RI, Midthun K, Gorziglia M, Chanock RM. Rotavirus the major etiologic agent of severe infantile diarrhea may be controlable by a "Jennerian"approach to vaccination. The Journal of Infectious Diseases 153:815-822, 1986.

[21] 21. Kapikian AZ, Flores J, Hoshino Y, Midthun K, Gorziglia M, Green KY, Chanock RM, Potash L, Sears SD, Clements ML, Haisey NA, Black RE, Peresschael I. Prospects for development of a rotavirus vaccine against rotavirus diarrhea in infants and young children. Reviews of Infectious Diseases 4:539-546, 1989.

[22] 22. KeswickBH, PickeringLK, DupontHL, Woodward WE. Prevalence of rotavirus in children in day care centers. Journal of Pediatrics 103:85-86, 1983.

[23] 23. Koopman JS, Monto AS. The tecumseh study XV: Rotavirus infection and pathogenicity. American Journal of Epidemiology 130:750-759, 1989.

[24] 24. Koopman JS, Monto AS, Longin IMJR. The tecumseh study XVI: Family and community sources of rotavirus infection. American Journal of Epidemiology 130:760-768, 1989.

[25] 25. LaemmliVK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-686, 1970.

[26] 26. Leite JPG, Pereira HG, Azevedo RS, Schatzmayr HG. Adenovirus in faeces of children with acute gastroenterite in Rio de Janeiro, Brazil. Journal of Medical Virology 15:203-207, 1985.

[27] 27. Louren< jo MH, Nicolas JC, Cohen J, Scherrer R, Bricout F. Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolate in France. Annals of Institute Pauster/ Virology 132:161-173, 1981.

[28] 28. Pereira HG, Azevedo RS, Leite JPG, Andrade AP, Castro LA, Combined enzyme immunoassay for rotavirus and adenovirus (EIARA). Journal of Virological Methods 10:21-28, 1985.

[29] 29. Pereira HG, Azevedo RS, Leite JPG, Barth OM, Sutmoller JF, Farias V, Vidal MMP. Comparison of polyacrylamid gel electrophoresis (PAGE), immuno- electron microscopy (IEM) and enzyme immunoassay (EIA) for the rapid diagnosis of rotavirus infection in children. Memórias do Instituto Oswaldo Cruz 78:483-490, 1983.

[30] 30. Pickering LK, Bartiett AV III, Reves RR, Morrow A. Asymptomatic excretion of rotavirus before and after rotavirus diarrhea in children in day care centers. Journal of Pediatrics 112:261-365, 1988.

[31] 31. Rácz ML, Candeias JAN, Trablusi JR, MurahowskiJ. Diarrheal disease in Brazil: Clinical features of rotavirus-associated gastroenteritis in children. European Journal of Epidemiology 4:382- 385, 1988.

[32] 32. Raphael RA, Sattar SA, Springthorpe VS. Longterm survival of human rotavirus in rawtreated river water. Canadian Journal Microbiology 31:124-128, 1985.

[33] 33. Rodrigues WJ, Kim HW, Brandt CD, Gardner MK, Parrott RH. Use of electrophoresis of RNA from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery. The Journal of Infectious Diseases 148:34-40, 1983.

[34] 34. Sattar SA, Ijas MK, Johson-Lussenburg CM, Springthorpe VS. Effect of relative humidity on the airborne survival of rotavirus SA-11. Apllied and Environmental Microbiology 47:879-881, 1984.

[35] 35. Tiemessen CT, Wegerhoff FO, Erasmus MJ, Kidd AH. Infection by enteric adenovirus, rotavirus and other agents in a rural african environment. Journal of Medical Virology 28:176-182, 1989.

Brasil

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Ocorrência de rota vírus e adeno vírus em crianças de até 11 anos de idade sem sintomatologia de diarréia em Goiânia - GO

Resumos

Endereço para correspondência:

Datas de Publicação

Histórico