First report of Tomato severe rugose virus infecting pepper plants in Brazil

Primeiro relato do Tomato severe rugose virus infectando plantas de pimentão no Brasil

PHYTOPATHOLOGICAL NOTES NOTAS FITOPATOLÓGICAS

First report of Tomato severe rugose virus infecting pepper plants in Brazil

Primeiro relato do Tomato severe rugose virus infectando plantas de pimentão no Brasil

Denise N. Nozaki^I; Renate Krause-Sakate^I; Jorge M. Hasegawa^I; Márcia A Cezar^I; Pedro H. Dziuba^II; Marcelo A. Pavan^I

^IDepartamento de Defesa Fitossanitária, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Universidade Estadual Paulista, Cx. Postal 237, CEP 18603-970, Botucatu, SP, e-mail: [e-mail]

^IIAPPI, Associação dos Plasticultores de Piraju, CEP 1880-000, Piraju, SP

RESUMO

Este trabalho relata a primeira ocorrência do Tomato severe rugose virus (ToSRV) em plantas de pimentão (Capsicum annum) no Brasil. O vírus foi detectado em plantas coletadas nos municípios de Paranapanema e Piraju, São Paulo. O ToSRV é um begomovírus típico, descrito originalmente em plantas de tomateiro (Lycopersicon esculentum) e até o presente não havia sido detectado em pimentão.

Viruses belonging to the Begomovirus genus, family Geminiviridae, are single stranded DNA viruses that infect dicotyledonous plants, are transmitted by a single species of whitefly (Bemisia tabaci Gennadius), and typically have genomes consisting of two components (Fauquet et al., Archives of Virology 148:405-421. 2003). These viruses cause significant crop losses worldwide, and in Brazil the most affected crops have been tomato (Lycopersicon esculentum Mill.) and bean (Phaseolus vulgaris L.).

During a survey for pepper (Capsicum annum L.) diseases in the Paranapanema and Pirajú regions of São Paulo state, plants from cultivars P36, Lilac and Mandarim showing mosaic symptoms were analyzed for the presence of a begomovirus. DNA was extracted (Dellaporta et al., Plant Molecular Biology Reporter 1:19-21. 1983) and PCR was carried out with the primer pair PrV324/PrC883, which amplifies most of the coat protein gene (Wyatt & Brown, Phytopathology 86:1288-1293. 1996). The 576 bp fragment for five isolates was sequenced and the deduced amino acid sequence identity ranged from 96-97% with Tomato severe rugose virus (ToSRV), a begomovirus species described infecting tomatoes in Minas Gerais and São Paulo. Although the definitive identity of the virus cannot be assured until the entire DNA-A is sequenced, the high degree of identity with ToSRV is a strong indication that this virus is indeed an isolate of ToSRV. Pepper plants grown under greenhouse conditions at these two different regions were infected with ToSRV.

This is the first report of ToSRV naturally infecting peppers in Brazil, and indicates that tomato-infecting begomoviruses can also be a constraint for pepper production.

Accepted for publication on 23/06/2005

Author for correspondence: Renate Krause Sakate

Publication Dates

• Publication in this collection
  04 Sept 2006
• Date of issue
  June 2006