Open-access PHENOLICS IN TWO Alternanthera SPECIES RESIDUES AFFECT THE GERMINATION AND EARLY SEEDLING GROWTH OF RICE (Oryza sativa)

Fenóis Totais em Resíduos de Duas Espécies de Alternanthera Afetam a Germinação e o Crescimento das Plântulas de Arroz (Oryza sativa)

ABSTRACT

Alternanthera species are invasive aquatic/semi-aquatic weeds posing a serious threat to agro-biodiversity in several countries in the world. The present study was conducted to assess the phytotoxic effects of Alternanthera philoxeroides and A. sessilis residues on emergence and early seedling growth traits of rice (Oryza sativa). Soil was prepared with 4% (w/w) Alternanthera species residues separately and allowed to decay for 0, 15 and 30 days. Rice emergence was significantly decreased but increase in mean emergence time and time to 50% emergence was observed in soils modified with Alternanthera species residues compared with seed sown in unmodified soils. Rice emergence was reduced to 50-67% and 52-75% by A. sessilis and A. philoxeroides, respectively. A significant reduction in rice root, shoot length, and biomass was also noted with Alternanthera-infested soil. Total phenolics increased with increasing residue decay time in both species thereby showing their direct interaction with emergence and seedling traits of rice. The phenolic compounds identified were namely Quercitin, Chlorogenic acid, P-Coumeric acid, Trans-4-hydroxy3-methoxy, Cinamic acid, Caffeic acid, Syringic acid, Sinapic acid, Vanillic acid, 4-hydroxy-3-methoxy benzoic acid.

Keywords: allelopathy; Alternanthera; rice; seedling growth; decomposition periods

RESUMO

As espécies do gênero Alternanthera são plantas daninhas aquáticas/semiaquáticas invasoras que representam uma ameaça grave para a agrobiodiversidade em diversos países do mundo. O presente estudo foi realizado para avaliar os efeitos fitotóxicos dos resíduos de Alternanthera philoxeroides e A. sessilis nas características de emergência e crescimento inicial de plântulas de arroz (Oryza sativa). O solo foi preparado com resíduos de espécies de Alternanthera – 4% (w/w) – separadamente, que foram submetidas a um período de decomposição de 0, 15 e 30 dias. Houve redução significativa da emergência do arroz, porém foi observado aumento no tempo médio de emergência e no tempo para emergência de 50% em solos modificados com resíduos de espécies de Alternanthera, em comparação com sementes semeadas em solos não modificados. A emergência do arroz com A. sessilis e A. philoxeroides foi reduzida para 50-67% e 52-75%, respectivamente. Uma redução significativa do comprimento da raiz do arroz, da parte aérea e da biomassa também foi observada no solo infestado com as espécies de Alternanthera. Também ocorreu aumento dos fenólicos totais à medida que aumentou o tempo de decomposição dos resíduos em ambas as espécies, mostrando, assim, interação direta com as características de emergência e crescimento de plântulas de arroz. Foram identificados os compostos fenólicos quercetina, ácido clorogênico, ácido p-cumárico, trans-4-hidroxi-3-metoxi, ácido cinâmico, ácido cafeico, ácido siríngico, ácido sinápico, ácido vanílico e ácido benzoico 4-hidroxi-3-metoxi.

Palavras-chave: alelopatia; Alternanthera; arroz; crescimento de plântulas; períodos de decomposição

INTRODUCTION

Weeds are one of the major problems in crop production (Marwat et al., 2008) as they compete with crop plants for nutrients, moisture, light, space, growth requirements, and exert allelopathic effects on crop seed germination and growth by releasing water-soluble compounds into the soil (Batish et al., 2007b; Kumar et al., 2009). A wide range of injurious effects on crop growth has been reported as a result of phytotoxic decomposing products, released from leaves, stem, roots, fruit and seeds (Khan et al., 2009). The allelopathic effect of a weed on crops can be ascertained by measuring their germination and growth, a technique known as plant bioassay. The extent of allelopathic inhibition on germination and seedling growth of crops varies with weed species (Hamayun et al., 2005). Most of the weed species have inhibitory effects on crops; yet some weed species also exhibit stimulatory effects. Studies have reported inhibitory effects of phenolics from aquatic weeds on germination, seedling growth and total biomass of wheat (Abbas et al., 2014).

Rice is an important staple food and cash crop in different countries in the world. It is estimated that average crop losses caused by weeds worldwide are 9.5% (Alam, 2003). In severe cases, yield losses may amount to more than 50%, depending on density, species, time of weed germination, weed infestation duration, space available for growth and other management practices (BRRI, 2006). Some emerging invasive weeds of rice are A. sessilis and A. philoxeroides, which seem to be more proliferating and problematic than existing weeds. Alternanthera philoxeroides and A. sessilis are commonly known as alligatorweed and sessile joyweed, respectively which belong to the family Amaranthaceae. Alternanthera philoxeroides is a perennial species that rarely sets seeds (Julien, 1995). It is known as an invasive species in many parts of the world (Julien et al., 1995), and it has a tremendous potential for vegetative reproduction (Julien et al., 1995; Sainty et al. 1998; Clements et al., 2011). It has the ability to grow in both aquatic and terrestrial habitats and in conservation and agricultural systems of tropical, subtropical and temperate regions (Julien and Stanley, 1999). Alternanthera philoxeroides is a noxious weed in Brazil (Barreto and Torres, 1999), Australia (Julien and Bourne, 1988; Milvain et al., 1995; Krake et al., 1999), New Zealand, UK (Arthington and Mitchell, 1986) and USA (Rhodes and Demont, 1983). Alternanthera philoxeroides is a problem weed in 10 crops in 30 countries, a serious or principal weed in eight of these countries and a major weed in others. In China, A. philoxeroides has been recognized as an invasive and troublesome weed in rice, corn, cotton, soybean (Lu et al., 2002; Ye et al., 2003) wheat, sweet potatoes, vegetables, and fruit trees (Tan, 1994a,b).

Alternanthera philoxeroides has the potential to devastate natural systems, agricultural areas and recreational areas through its interference and spread by human activities. It is competitive with other plant species, forms monocultures, and is not constrained by natural enemies or other environmental constraints that exist in its native range (Bassett, 2009). The allelopathic potential of A. philoxeroides in its successful invasion of new areas has been reported by Xie et al. (2010). Paria and Mukharjee (1981) reported that A. philoxeroides contains allelopathic potential and complete inhibition of rice germination, and seedling growth was noted at 1:2.5 concentrations. Alternanthera philoxeroides caused 60% reduction in total biomass of wheat seedling (Abbas et al., 2014). Yong et al. (2011) reported inhibitory effects of A. philoxeroides in different field crops. In China, crop production was reduced by 20 to 63 percent due to A. philoxeroides (Ensbey, 2001) while A. philoxeroides invasion may lead to yield losses of up to 45% for some horticultural crops (Shen et al., 2005). In pasture ecosystems, A. philoxeroides steadily increases in biomass and displaces other species (Julien and Bourne, 1988). Aqueous extracts of A. philoxeroides inhibited the germination and seedling development of ryegrass (Zhang et al., 2009).

Alternanthera sessilis exists as a noxious weed in both wetlands and uplands (Soerjani et al., 1987), and it can grow on a variety of soil types. Reproduction in A. sessilis occurs by seeds. Alternanthera sessilis is the predominant weed of rice in Taiwan and causes moderate yield and quality losses, and it is economically important in certain other rice producing countries (Chiang and Leu, 1981). Abbas et al. (2014) reported that the water extract of A. philoxeroides and A. sessilis reduced up to 60% germination, seedling growth and total biomass of wheat. Information about alleopathic and residue decomposition effects of A. sessilis and A. philoxeroides on rice is lacking. It is hypothesized; that Alternanthera residues may hinder the germination and seedling growth traits of rice plants by releasing water soluble allelochemicals. Therefore, the objective of the study was to assess the phytotoxicity of A. sessilis and A. philoxeroides decomposed residues on germination and early seedling growth of rice.

MATERIALS AND METHODS

Collection of plant materials

Fully grown plants of A.philoxeroides and A. sessilis from an infested area were collected in 2013. These were separated into root, shoot, leaf, flower (only in A. sessilis) and whole plant fractions. These plant parts were cut into small pieces (2-3 cm) with a pair of scissors and air dried for a month under shade with mean temperature of 20 oC and relative humidity of 55%. The dried plant fractions were stored in plastic bags at room temperature before they were used for the experiments. For bioassay studies, certified seeds of rice variety “Basmati-515” were used.

Treatments and experimental design

There were seven treatments for the germination bioassay, arranged in a completely randomized design with four replications. The treatments included control, A. sessilis (0, 15 and 30 days decomposition) and A. philoxeroides (0, 15 and 30 days decomposition). Maximum and minimum temperatures during the course of the experiment were 35.1 oC and 33.8 oC, respectively.

Seedling emergence and growth bioassay with Alternanthera residues decomposed for 15 and 30 days

In order to simulate natural conditions, a study was conducted in which concentrations of 1, 2, 3, 4, and 5% (w/w) of A. philoxeroides and A. sessilis residues were mixed in 250 g soil for each Alternanthera species, separately. It was found that there was no rice germination at the 5% (w/w) concentration for either species. Hence a new study was planned to study the germination and seedling growth of rice using 4% residues of Alternanthera species. In the present study, 8 g residues of both Alternanthera species were mixed into the soil of filled plastic pots of 10 cm diameter and 10 cm depth to get 4% (w/w) for each Alternanthera species separately. Then 100 mL of distilled water was added to each pot and left at room temperature to decompose the residues for 0, 15, and 30 days. In control, only 200 g of unmodified soil was used. Ten seeds of rice were sown simultaneously in each plastic pot. In order to determine the type and amount of phenolic compounds in the soil with decomposed residues, separate pots with the same amount of weed residues of both weed species were placed and allowed to decompose for 15 and 30 days. All the pots were placed on a laboratory bench. The samples were taken from the pots having only weed residues of both weed species decomposed for a period of 15 and 30 days and analyzed for determination of phenolic compounds.

Data collection and statistical analysis

Mean Germination Time (MGT) was calculated with the equation of Ellis and Roberts (1981).

M G T = Σ D n / Σ n

where n is the number of seeds that had germinated on day D and D is the number of days counted from the beginning of germination.

Time taken to 50% germination (T50) was calculated by using the formula given by Coolbear et al. (1984) as modified by Farooq et al. (2004).

T 50 = t i + N 2 - n i t j - t i n j - n i

where N is the final number of germinated seeds while nj and ni are the cumulative number of seeds germinated by adjacent counts at times tj and ti, respectively, where ni< N/2 <nj. Germination index (GI) was calculated as given by the Association of Official Seed Analysis (AOSA, 1990). Germination was calculated by counting and removing the germinated seeds. Germination was observed daily in accordance with the methods of the Association of Official Seed Analysis (AOSA, 1990).

Total soluble phenolics were determined as described by Randhir and Shetty (2005) and were expressed as gallic acid equivalents (Figure 2). For identification and quantification of their suspected phytotoxins, aqueous extracts were chemically analyzed (Table 3) on Shimadzu HPLC system (Model SCL-10A, Tokyo, Japan). The peaks were detected by UV detector. Standards of suspected phytotoxins (Aldrich, St Louis, USA) were run similarly for their identification and quantification. Concentration of each isolated compound was determined by the following equation.

Concentration (ppm) = Area of the sample Area of the standard × Concentration of the standard × Dilution factor

Figure 1
Effect of duration of Alternanthera residue decomposition on emergence % of rice.

Figure 2
Release of total phenolics by Alternanthera residues after different decomposition durations.

Seedling vigor index (SVI) was calculated by using the following formula of Abdul-Baki and Anderson (1973).

S V I = g e r m i n a t i o n / e m e r g e n c e % × r a d i c l e l e n g t h

Each experiment was repeated twice. The average data obtained from each experiment were subjected to analysis of variance using the computer software statistix 8.1. The treatment means were grouped on the basis of least significant difference at the 0.05 level of probability (Steel et al., 1997).

Regression analyses as well as the figures were completed using the software Minitab 16 (Minitab, State College, PA) in which linear, quadratic and cubic components were successively tested for significance and included if the residual sum of squares were significantly reduced (p<0.05). The most appropriate regression model between decomposition duration (Alternanthera sessilis and Alternanthera philoxeroides) and mean emergence time, emergence percentage, shoot length, shoot dry weight, root length and root dry weight was the second order quadratic one (Equation 1).

Y = β 0 + β 1 x + β 2 x 2 + β 3 x 3 + ε (eq. 1)

where Y is the dependent (response) variable, x is the independent variable, and the error term ε is assumed to have normal distribution with constant variance. For each response, the validity of model assumptions was verified by examining the residuals as described in Montgomery (2009). Contour plots of shoot dry weight and root dry weight for mean emergence time (days) and seedling vigour index of rice were constructed using the software Minitab 16 (Minitab, Sate College, PA)

RESULTS AND DISCUSSION

Data in Table 1 show that Alternanthera sessilis and A. philoxeroides modified soil significantly and affected the time taken to 50% seed emergence (T50) and mean emergence time (MET). Both were significantly increased in comparison with unmodified soil (Table 1). The highest emergence index (EI) value was recorded in unmodified soil, which was significantly higher than the values of all other treatments, while the lowest values of EI were recorded in treatments where Alternanthera species residues were allowed to decompose for 30 days. The contrast Alternanthera sessilis vs A. philoxeroides for MET and the contrast 0 vs 15 days weed residue decomposition for EI were non significant. The remainder of rice seed germination traits showed significant differences for A. sessilis vs A. philoxeroides, for the durations of 0 vs 15, 0 vs 30 and 15 vs 30 day residues decomposition. Figure 1 shows that emergence (%) of rice was significantly affected by both weed species residues and drastically reduced by increasing the duration of weed residue decomposition. Our study indicates that Alternanthera residues have inhibitory effects on emergence of rice seedlings and there was an increase in phytotoxicity with increased duration of Alternanthera residue decomposition. It could be related to release of more allelochemicals and their availability in soil. These results are supported by the findings of Tanveer et al. (2010).

Table 1
Effect of duration of Alternanthera residue decomposition on emergence traits of rice

They recorded significant reduction in emergence of wheat, chickpea and lentil in Euphorbia helioscopia infested soil.

Delayed emergence might be due to the production of the phenolic compound vanillic acid, which has inhibitory effect on germination (Ali et al., 2013). Although emergence was delayed with the decomposition period of 30 days, it occurred much earlier than the 15-day decomposition period. This can be attributed to the different phenolic produced at these two decomposition periods. The differences in the emergence time of rice for the two Alternanthera species can be due to production of more phenolics by A. philoxeroides than A. sessilis (Table 3).

Alternanthera sessilis and A. philoxeroides residues, after the decomposition period of 15 days, enhanced root length, shoot length, root dry weight, shoot dry weight and seedling vigor index (SVI) of rice as compared to other treatments except for the control (Table 2). A significant inhibitory effect of A. sessilis and A. philoxeroides on rice seedling root, shoot length and their dry weights were recorded when these weed residues were allowed to decompose for zero and 30 days. The contrast comparison between control vs all periods was highly significant for root, shoot length, their dry weights and SVI whereas it was non-significant between both weed species for root dry weight. The contrast comparison between 0 vs 15-day decomposition was non-significant for SVI of rice. Contrasts between 0 vs 30 and 15 vs 30-day residue decomposition were significant for all seedling growth parameters (Table 2). These results indicate that Alternanthera residues release growth retardatory substances into the soil, which accumulate in bioactive concentrations and adversely affect the growth of rice seedlings. Increase in seedling growth of rice at 15-day decomposition duration of A. sessilis and A. philoxeroides residues could be attributed to the presence of allelochemicals which had stimulatory effect (Kadioglue et al., 2005).

Table 2
Effect of duration of Alternanthera residue decomposition on seedling growth of rice

The relationship between decomposition duration (A. sessilis and A. philoxeroides) and rice responses (mean emergence time, emergence percentage, shoot length, shoot dry weight, root length and root dry weight) was adequately described by the second order polynomial regression model, whose relationship between decomposition duration and rice responses was very strong (R2 ranges from 0.84 to 0.99), suggesting that the fitted models shown in Figures 3 and 4 can be used to predict rice responses at decomposition duration from 0 to 30 days.

Figure 3
Effect of decomposition duration of Alternanthera.sessilis on mean emergence time, emergence (%), shoot/root length and shoot/root dry weight of rice. The plots were fitted with second order polynomial regression models. Equations of the fitted models, P-values and R2 values are shown within each plot.

Figure 4
Effect of decomposition duration of Alternanthera.philoxeroides on mean emergence time, emergence (%), shoot/root length and shoot/root dry weight of rice. The plots were fitted with second order polynomial regression models. Equations of the fitted models, P-values and R2 values are shown within each plot.

Table 3
Water soluble phenolics identified in Alternanthera residue extracts

Mean emergence time of rice increased with decomposition duration of A. sessilis from 0 to 15 days and decreased from 15 to 30 days (Figure 3A). However, in case of A. philoxeroides, mean emergence time of rice continued to increase from 0 to 30 days of decomposition duration (Figure 4A). There was a strong relationship between emergence percentage (decreased) of rice and decomposition duration of A. sessilis from 0 to 30 days analyzed through regression (Figure 3B). Decomposition duration (0 to 15 days) of A. philoxeroides increased emergence percentage of rice but emergence percentage decreased from 15 to 30-day duration (Figure 4B). Shoot length, shoot dry weight, root length and root dry weight of rice increased with the increasing decomposition duration (0-15 days) of A. sessilis and A. philoxeroides and steeply decreased from 15 to 30 days, exhibiting a strong quadratic relationship (Figs. 3C, 3D, 3E, 3F, 4C, 4D, 4E and 4F). Allelochmicals have potential to stimulate growth at their lower concentrations. It may possible that by increasing the time of decomposition, the concentration of allelochemicals was increased from lower to higher. After fifteen days, lower concentration of allelochmicals might be equated with stimulatory doses. At zero days, no allelochemicals were released,; however, 15 days after decomposition, allelopathic effects may be stimulatory or inhibitory (Torres et al., 1996); most of the allelochemicals produced inhibitory effects at higher concentrations and stimulated growth at lower concentrations (Saleh and Madany, 2013; Hernandez-aro et al., 2016)

The contour plot (Figure 5) showed how seedling vigor index (y) and mean emergence time (x) affect the shoot/root dry weight (contours) of rice. The darker regions indicate higher shoot/ root dry weight. The contour levels reveal a peak centered in the vicinity of 6 days (mean emergence time) and 390 (seedling vigor index). Shoot and root dry weights in this peak region are greater than 30 g and 8 g, respectively. The seedling exhibiting higher vigor index emerged earlier and had more time for accumulating root and shoot biomass. The increase in root and shoot biomass with increased mean emergence time has been reported by Mehmood et al. (2014).

Figure 5
Contour plot of shoot dry weight (A) and root dry weight (B) for mean emergence time (days) and seedling vigor index of rice.

The present study indicates that rice can grow well under normal conditions of soil but is sensitive to soil amendment with Alternanthera residues. The reduction in emergence, seedling length, dry weight and increase in emergence time of rice seedlings could be attributed to the presence of phytotoxic phenolics in the amended soil. These have been identified as Quercitin, Chlorogenic acid, P-Coumaric acid, Trans-4-hydroxy3-methoxy cinamic acid, Caffeic acid, Syringic acid, Vanillic acid, and 4-hydroxy-3-methoxy benzoic acid (Table 3).

Furthermore, total released phenolics significantly increased (from 25 to 55 μg g-1 in A. sessilis and 36 to 72 μg g-1 in A. philoxeroides) with increasing duration of Alternanthera residue decomposition (Figure 2). The presence of significantly higher amounts of phenolics after 30 days of decomposition of Alternanthera residues may be responsible for more reduction in rice seed emergence and seedling traits than those of 15-day decomposition. These results are in contradiction with those of Mersie and Singh (1987), who stated that toxicity of Parthenium residues to wheat diminished with increasing decomposition period and as a result, the residues decomposed for four weeks were less toxic than the undecomposed residues. This could be explained by the fact that allelopathic potential may vary in in different weed species. Abbas et al. (2014) identified chlorogenic acid, ferulic acid and m-coumaric acid in Conyza stricta; m-coumaric acid, p coumaric acid and vanilic acid in Echinocloa crus-galli; caffeic acid, chlorogenic acid, m-coumaric acid and p-coumaric acid in Polygonum barbatum. The presence of significantly higher amounts of phenolics (Figure 1 and Table 3) may be the reason for a greater effect of A. philoxeroides than A. sessilis on rice emergence and seedling traits. The results are supported by Paria and Mukherjee (1981), who recorded complete inhibition of mustard and rice seed germination and seedling growth with leaf extract of A. philoxeroides.

Thus, based on the present study, it could be concluded that phytotoxic phenolics in residues of A. philoxeroides and A. sessilis affect the emergence and growth of rice seedlings.

ACKNOWLEDGEMENT

The authors are thankful to the financial support of the Higher Education Commission, Government of Pakistan under the Indigenous Ph.D. Fellowship Program (5000 fellowship).

REFERENCES

  • Abbas T. et al. Allelopathic effects of aquatic weeds on germination and seedling growth of wheat. Herbologia. 2014;14:11-25.
  • Abdul-Baki B.A.A., Anderson J.D. Relationship between decarboxylation of glutamic acid and vigour in soybean seed. Crop Sci. 1973;13:222-6.
  • Alam S.M. Weeds and their ill effects on main crops. Dawn the internet edition http://DAWN.com 2003.
    » http://DAWN.com
  • Ali H.H. et al. Germination ecology of Rhynchosia capitata: an emerging summer weed in Asia. Planta Daninha. 2013;31:249-57.
  • Arthington A.H., Mitchell D.S. Aquatic invading species. In: Groves RH.; Burdon J.J., editors.). Ecology of biological invasions. London: Cambridge University Press; 1986. p.34-56.
  • Association of Official Seed Analysis - AOSA. Rules for testing seeds. J Seed Technol. 1990;12:1-112.
  • Barreto R.W., Torres A.N.L. Nimbya alternanthera and Cercospora alternanthera two new records of fungal pathogen on A. philoxeroides (alligator weed). Austr Plant Pathol J. 1999;28:103-7.
  • Bassett E.I. Ecology and management of alligator weed, Alternanthera philoxeroides [tese]. Auckland: University of Auckland; 2009.
  • Batish D.R. et al. Root-mediated allelopathic interference of nettle-leaved goosefoot (Chenopodium murale) on wheat (Triticum aestivum). J Agron Crop Sci. 2007b;193:37-44.
  • Bangladesh Rice Research Institute - BRRI. Weed identification and management in rice. Joydebpur: Bangladesh Rice Research Institute; 2006.
  • Chiang, M.Y., Leu, I.S. Weeds in paddy field and their control in Taiwan. Weeds and Weed Control in Asia. Taiwan: Food and Fertiliser Technology Center; 1981.
  • Clements D. et al. Growth of aquatic alligator weed (Alternanthera philoxeroides) over 5 years in south-east Australia. Aquatic Invas. 2011;6:77-82.
  • Coolbear P. et al. The effect of low temperature presowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. J Exper Bot. 1984;35:1609-17.
  • Ellis R.A., Roberts E.H. The quantification of aging and survival in orthodox seeds. Seed Sci Technol. 1981;9:373-409.
  • Ensbey R. Alligator weed. Agfact P7.6.46. 2nd ed. Melbourne: NSW Agriculture; 2001.
  • Farooq M. et al. Influence of high and low temperature treatments on the seed germination and seedling vigor of coarse and fine rice. Inter Rice Res Notes. 2004;29:p.69-71.
  • Hamayun M. et al. Allelopathic effects of Cyperus rotundus and Echinochloa crusgalli on seed germination, plumule and radical growth in maize (Zea mays L.). Pakistan J Weed Sci Res. 2005;11:81-4.
  • Julien M.H. Alternanthera philoxeroides (Mart.) Griseb. In: Groves RH, Shepherd RCH, Richardson RC, editors The biology of australian weeds. Frankston: R.G. and F.J. Richardson; 1995. p.1-12.
  • Julien M.H., Bourne A.S. Alligator weed is spreading in Australia. Plant Protec Quart. 1988;3:91-6.
  • Julien M.H., Stanley J.N. The management of alligator weed, a challenge for the new millennium. Proceedings of the 10th Biennial Noxious Weeds Conference, Ballina: 1999. p.2-13.
  • Kadioglu I. et al. Allelopathic effects of weeds extracts against seed germination of some plants. J Environ Biol. 2005;26:169-73.
  • Khan A.L. et al. Assessment of Allelopathic potential of selected medicinal plants of Pakistan. African J Biotechnol. 2009;8:1024-9.
  • Krake K. et al. Emerging aquatic weeds. In: Proceedings of Aquatic weeds workshop held at Keith Turnbull Research Institute, Frankston, Victoria, Australia. by the Weed Science Society of Victoria. Plant Protec Quart. 1999;14:79.
  • Kumar V. et al. Suppression of Powell Amaranth (Amaranthus powellii) by Buckwheat Residues: Role of Allelopathy. Weed Sci. 2009;57:66-73.
  • Lu Y.L. et al. Research status quo on alligator weed in China. J Jianshu Agric.2002;4:46-8.
  • Marwat K.B. et al. Study of various herbicides for weed control in wheat under irrigated conditions. Pakistan J Weed Sci Res. 2008;14:1-9.
  • Mehmood A. et al. Comparative allelopathic potential of metabolites of tow Alternanthera species against germination and seedling growth of rice. Planta Daninha. 2014;32:1-10.
  • Mersie W., Singh M. Allelopathic effect of Parthenium (Parh;enium hysterophorus L.) extract and residue on some agronomic crops and weeds. J. Chem. Ecol. 1987;13:1739-47.
  • Milvain H. et al. Alligator weed MIA campaign. Has it been a success? Better planning for better weed management. In: Proceedings of the 8th biennial noxious weeds conference. Goulburn: 1995. p.87-9.
  • Montgomery D.C. Design and analysis of experiments. 7th ed. New York: Wiley; 2009.
  • Paria N., Mukherjee, A. Allelopathic potenetial of a weed, Alternanthera philoxeroides (Mart.) Griseb. Bangladesh J Bot. 1981;10:86-9.
  • Randhir R., Shetty K. Developmental stimulation of total phenolics and related antioxidant activity in light and dark germinated maize by natural elicitors. Process Biochem. 2005;40:1721-32.
  • Rhodes G.N. Jr., Demont D.J. Aquatic weed management perspectives in North Carolina. In: Proceedings of the 36th Southern Weed Science Society, Annual Meeting. Southern Weed Science Society, 1983. 321p.
  • Sainty G. et al. Control and spread of alligator weed, Alternanthera philoxeroides, in Australia: lessons for other regions. Wetlands Ecol Manage. 1988;5:195-201.
  • Shen J. et al. Effect of environmental factors on shoot emergence and vegetative growth of alligator weed (Alternanthera philoxeroides). Weed Sci. 2005;53:471-8.
  • Soerjani M. et al. Weeds of rice in Indonesia. Balai Pustaka, Jakarta: 1987.716p.
  • Steel R.G.D. et al. Principles and procedures of statistics: a biometrical approach. 3rded. New York: McGraw Hill Book, 1997. p.172-7.
  • Tan W.Z. Yield losses of different crops caused by alligatorweed (Alternanthera philoxeroides. Chinese J Weed Sci. 1994a;8:28-32.
  • Tan W.Z. Horizontal and vertical distributions of alligator weed (Alternanthera philoxeroides) in china. Chinese J Weed Sci. 1994b;9:30-4.
  • Tanveer A. et al. Allelopathic potential of Euphorbia helioscopia L. against wheat (Triticum aestivum L.), chickpea (Cicer arietinum L.) and lentil (Lens culinaris Medic.). Turkian J Agric For. 2010;34:75-81.
  • Torres A. et al. First world congress on allelopathy. The science of the future. SAI (University of Cadiz), Spain. 1996: 278.
  • Xie L.J. et al. Allelochemical mediated invasion of exotic plants in China. Allelopathy J. 2010;25:31-50.
  • Ye W.H. et al. Genetic uniformity of Alternanthera philoxeroides in South China. Weed Res. 2003;43:297-302.
  • Yong X. et al. The Study on Allelopathy Mechanismof Aqueous Extracts from the Different Organizations of Alternanthera philoxeroides Griseb. on Vicia faba and Zea mays. Chinese Agric Sci Bull. 2011;27:158-63.
  • Zhang Z. et al. Allelopathic effects of tissue extract from alligator weed on seed and seedling of ryegrass. Acta Bot BorealiOccidentalia Sin. 2009;29:148-53.

Publication Dates

  • Publication in this collection
    2017

History

  • Received
    24 Nov 2015
  • Accepted
    23 June 2016
location_on
Sociedade Brasileira da Ciência das Plantas Daninhas Departamento de Fitotecnia - DFT, Universidade Federal de Viçosa - UFV, 36570-000 - Viçosa-MG - Brasil, Tel./Fax::(+55 31) 3899-2611 - Viçosa - MG - Brazil
E-mail: rpdaninha@gmail.com
rss_feed Acompanhe os números deste periódico no seu leitor de RSS
Acessibilidade / Reportar erro