Variação da composição química do meio de cultura e da bioquímica celular de Phaeodactylum tricomutum (Bohlin), em cultivos estanques

Aidar, Elizabeth; Ehrlich, Roberto; Asano, Cristina Sayuri; Sigaud, Teresa Cristina Siqueira

doi:10.1590/S0373-55241991000200004

Resumo

P. tricornutum was grown in batch cultures under 21ºC temperature and continuous irradiance of 183 µE.m-2 . s-1, provided by fluorescent light tubes. Cultures growth were followed by cell countings, "in vivo" fluorescence and cellular protein, carbohydrate and chlorophyll-α content. The maximum value of protein/carbohydrate ratio was found at the exponential phase and the minimum value at the stationary phase. This ratio characterizes the physiological stage of the culture. Cell uptake rates for nitrate, ammonium and phosphate from the media were higher during the lag-phase. Nitrite excretion by the cells were detected following nitrate uptake.

Phaeodactylum tricornutum; Algae culture; Chlorophylls; Proteins; Nutrients (mineral); Carbohydrates

Phaeodactylum tricomutum; Cultivo de algas; Clorofila-a; Proteínas; Nutrientes inorgânicos; Carboidratos

ARTIGOS

Variação da composição química do meio de cultura e da bioquímica celular de Phaeodactylum tricomutum (Bohlin), em cultivos estanques

Long term changes in the culture medium composition and cellular biochemistry of Phacodactylum tricomutum (Bohlin), in batch cultures

Elizabeth Aidar; Roberto Ehrlich; Cristina Sayuri Asano; Teresa Cristina Siqueira Sigaud

Instituto Oceanográfico da Universidade de São Paulo (Caixa Postal 9075, 01065-970 São Paulo, SP, Brasil)

Descritores: Phaeodactylum tricomutum, Cultivo de algas, Clorofila-a, Proteínas, Nutrientes inorgânicos, Carboidratos.

ABSTRACT

P. tricornutum was grown in batch cultures under 21ºC temperature and continuous irradiance of 183 µE.m^-2 . s^-1, provided by fluorescent light tubes. Cultures growth were followed by cell countings, "in vivo" fluorescence and cellular protein, carbohydrate and chlorophyll-α content. The maximum value of protein/carbohydrate ratio was found at the exponential phase and the minimum value at the stationary phase. This ratio characterizes the physiological stage of the culture. Cell uptake rates for nitrate, ammonium and phosphate from the media were higher during the lag-phase. Nitrite excretion by the cells were detected following nitrate uptake.

Descriptors: Phaeodactylum tricornutum, Algae culture, Chlorophylls, Proteins, Nutrients (mineral), Carbohydrates.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Agradecimentos

Os autores agradecem às facilidades de laboratório oferecidas pelo Instituto Oceanográfico, bem como o apoio financeiro do CNPq a um dos autores (E. Aidar).

Referências bibliográficas

AIDAR-ARAGÃO, E. 1980. Alguns aspectos da autoecologia de Skeletonema costatum (Greville) Cleve, de Cananéia (25ºS - 48ºW), com especial referência ao fator salinidade. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 2v.

AMINOT, A. & CHAUSSEPIED, M. 1983. Manuel des analyses chimiques en milieu marin. Brest, Centre National pour L'Exploitation des Oceans. 395 p.

BEN-AMOTZ, A. & GILBOA, A. 1980. Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar. Ecol.-Progr. Ser., 2:221-224.

BRAARUD, T. 1961. Cultivation of marine organisms as a means of understanding environmental influences on populations. In: Sears, M., ed. Oceanography. Washington, DC : American Association for Advancement of Science Publication, 67:271- 298.

COLLOS, Y. 1986. Time-lag algal growth dynamics: biological constraints on primary production in aquatic environments. Mar. Ecol.-Prog. Ser., 33:193-206.

DROOP, M. R. 1958. Optimun relative and actual ionic concentrations for growth of some euryhaline algae. Verh. int. Ver. Limnol., 13:722-730.

______ 1975. The nutrient status of algal cells in batch culture. J. mar. biol. Ass. U.K., 55:541-555.

DUBOIS, M.; GILLES, K. A.; HAMILTON, J. K.; REBERS, P. A. & SMITH, F. 1956. Colorimetric method for determination of sugars and related substances. Analyt. Chem., 18:350-356.

FABREGAS, J.; ABALDE, J.; HERRERO, C; CABEZAS, B. & VEIGA, M. 1984. Growth of the marine microalga Tetraselmis suecica in batch cultures with different salinities and nutrient concentrations. Aquaculture, 42:207-215.

FAWLEY, M. W. 1984. Effects of light intensity and temperature interactions on growth characteristics of Phaeodactylum tricomutum (Bacillariophyceae). J. Phycol., 20:67-72.

GAETA, S. A. 1985. Comparação das respostas de crescimento e fotossíntese de três clones de Phaeodactylum tricornutum Bohlin. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 106p.

______ 1987. Efeitos do mercúrio (HgC₁₂) sobre o crescimento e fotossíntese de Phaeodactylum tricornutum Bohlin. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 234 p.

GOLDMAN, J. C. & GLIBERT, P. M. 1983. Kinetics of inorganic nitrogen uptake by phytoplankton. In: Carpenter, E. J. & Capone, D. G., eds Nitrogen in the marine environment. New York, Academic Press, p.233-274.

GRASSHOFF, K.; EHRHARDT, M. & KREMLING, K. 1983. Methods of seawater analysis. 2nd revised and extended ed. Weinhein, Verlag Chemie. 419 p.

GUILLARD, R. R. L. & RYTHER, J. H. 1962. Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol., 8:229-239.

HARVEY, H. W. 1939. Substances controlling the growth of a diatom. J. mar. biol. Ass. U.K., 23:499-520.

KAIN, J. M. & FOGG, G. E. 1958. Studies on the growth of marine phytoplankton. I. Asterionella japonica Gran. J. mar. biol. Ass. U.K., 37:397-413.

LAING, I. 1985. Growth response of Chaetoceros calcitrans (Bacillariophyceae) in batch culture to a range of initial silica concentrations. Mar. Biol., 85:37-41.

LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. 1951. Protein measurement with the folin phenol reagent. J. biol. Chem., 193:265-275.

MYKLESTAD, S. & HAUG, A. 1972. Production of carbohydrates by the marine Chaetoceros affinis var. Willei (Gran.) Hustedt. I. Effect of the concentration of nutrients in the culture medium. J. exp mar. Biol. Ecol., 9:125-136.

PRÉZELIN, B. B. 1981. Light reactions in photosynthesis. In: Platt, T. ed. Physiological bases of phytoplankton ecology. Can. Bull. Fish, aquat. Sci., (210):1-43.

PRIBIL, S. & MARVAN, P. 1979. Batch cultivation. In: Marvan, P., Pribil, S. & Lhotsy, O. eds Algal assays and monitoring eutrophication. Stuttgart, E. Schweizerbart'sche Verlagsbuchhandlung (Ngele u. Obermiller), p.175-180.

PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-428.

RAIMBAULT, P. 1986. Effect of temperature on nitrite excretion by three marine diatoms during nitrate uptake. Mar. Biol., 92 (2):149-155.

SASSI, R.; KUTNER, M. B. B. & MOURA, G. F. 1988. Studies on the decomposition of drift seaweed from the northeast Brazilian coastal reefs. Hydrobiologia, 157:187-192.

SIGAUD, T. C. S. 1990. Efeitos de temperatura e salinidade sobre as respostas de crescimento e o conteúdo de clorofila-a de algumas espécies de algas planctónicas, em cultura. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 352 p.

STEEMANN-NIELSEN, E. 1978. Principal aspects concerning the batch technique in algal assays. Mitt, int. Verein. Theor. angew. Limnol., 21:81-87.

STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):p.1-311.

VIEIRA, A. A. H. 1975. Estudos experimentais em fitoplâncton marinho. Culturas e aspectos ecofisiológicos. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 162 p.

(Manuscrito recebido 18 de Fevereiro 1991; revisto 13 Junho e 13 Setembro 1991; aceito 11 Novembro 1991)

AIDAR-ARAGÃO, E. 1980. Alguns aspectos da autoecologia de Skeletonema costatum (Greville) Cleve, de Cananéia (25şS - 48şW), com especial referência ao fator salinidade. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 2v.
AMINOT, A. & CHAUSSEPIED, M. 1983. Manuel des analyses chimiques en milieu marin. Brest, Centre National pour L'Exploitation des Oceans. 395 p.
BEN-AMOTZ, A. & GILBOA, A. 1980. Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar. Ecol.-Progr. Ser., 2:221-224.
BRAARUD, T. 1961. Cultivation of marine organisms as a means of understanding environmental influences on populations. In: Sears, M., ed. Oceanography. Washington, DC : American Association for Advancement of Science Publication, 67:271- 298.
COLLOS, Y. 1986. Time-lag algal growth dynamics: biological constraints on primary production in aquatic environments. Mar. Ecol.-Prog. Ser., 33:193-206.
DROOP, M. R. 1958. Optimun relative and actual ionic concentrations for growth of some euryhaline algae. Verh. int. Ver. Limnol., 13:722-730.
______ 1975. The nutrient status of algal cells in batch culture. J. mar. biol. Ass. U.K., 55:541-555.
DUBOIS, M.; GILLES, K. A.; HAMILTON, J. K.; REBERS, P. A. & SMITH, F. 1956. Colorimetric method for determination of sugars and related substances. Analyt. Chem., 18:350-356.
FABREGAS, J.; ABALDE, J.; HERRERO, C; CABEZAS, B. & VEIGA, M. 1984. Growth of the marine microalga Tetraselmis suecica in batch cultures with different salinities and nutrient concentrations. Aquaculture, 42:207-215.
FAWLEY, M. W. 1984. Effects of light intensity and temperature interactions on growth characteristics of Phaeodactylum tricomutum (Bacillariophyceae). J. Phycol., 20:67-72.
GAETA, S. A. 1985. Comparação das respostas de crescimento e fotossíntese de três clones de Phaeodactylum tricornutum Bohlin. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 106p.
¹²
______ 1987. Efeitos do mercúrio (HgC₁₂) sobre o crescimento e fotossíntese de Phaeodactylum tricornutum Bohlin. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 234 p.
GOLDMAN, J. C. & GLIBERT, P. M. 1983. Kinetics of inorganic nitrogen uptake by phytoplankton. In: Carpenter, E. J. & Capone, D. G., eds Nitrogen in the marine environment. New York, Academic Press, p.233-274.
GRASSHOFF, K.; EHRHARDT, M. & KREMLING, K. 1983. Methods of seawater analysis. 2nd revised and extended ed. Weinhein, Verlag Chemie. 419 p.
GUILLARD, R. R. L. & RYTHER, J. H. 1962. Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol., 8:229-239.
HARVEY, H. W. 1939. Substances controlling the growth of a diatom. J. mar. biol. Ass. U.K., 23:499-520.
KAIN, J. M. & FOGG, G. E. 1958. Studies on the growth of marine phytoplankton. I. Asterionella japonica Gran. J. mar. biol. Ass. U.K., 37:397-413.
LAING, I. 1985. Growth response of Chaetoceros calcitrans (Bacillariophyceae) in batch culture to a range of initial silica concentrations. Mar. Biol., 85:37-41.
LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. 1951. Protein measurement with the folin phenol reagent. J. biol. Chem., 193:265-275.
MYKLESTAD, S. & HAUG, A. 1972. Production of carbohydrates by the marine Chaetoceros affinis var. Willei (Gran.) Hustedt. I. Effect of the concentration of nutrients in the culture medium. J. exp mar. Biol. Ecol., 9:125-136.
PRÉZELIN, B. B. 1981. Light reactions in photosynthesis. In: Platt, T. ed. Physiological bases of phytoplankton ecology.
Can. Bull. Fish, aquat. Sci., (210):1-43.
PRIBIL, S. & MARVAN, P. 1979. Batch cultivation. In: Marvan, P., Pribil, S. & Lhotsy, O. eds Algal assays and monitoring eutrophication. Stuttgart, E. Schweizerbart'sche Verlagsbuchhandlung (Ngele u. Obermiller), p.175-180.
PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-428.
RAIMBAULT, P. 1986. Effect of temperature on nitrite excretion by three marine diatoms during nitrate uptake. Mar. Biol., 92 (2):149-155.
SASSI, R.; KUTNER, M. B. B. & MOURA, G. F. 1988. Studies on the decomposition of drift seaweed from the northeast Brazilian coastal reefs. Hydrobiologia, 157:187-192.
SIGAUD, T. C. S. 1990. Efeitos de temperatura e salinidade sobre as respostas de crescimento e o conteúdo de clorofila-a de algumas espécies de algas planctónicas, em cultura. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 352 p.
STEEMANN-NIELSEN, E. 1978. Principal aspects concerning the batch technique in algal assays. Mitt, int. Verein. Theor. angew. Limnol., 21:81-87.
STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):p.1-311.
VIEIRA, A. A. H. 1975. Estudos experimentais em fitoplâncton marinho. Culturas e aspectos ecofisiológicos. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 162 p.

Datas de Publicação

Publicação nesta coleção
14 Maio 2012
Data do Fascículo
1991

Histórico

Recebido
18 Fev 1991
Revisado
13 Set 1991
Aceito
11 Nov 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] AIDAR-ARAGÃO, E. 1980. Alguns aspectos da autoecologia de Skeletonema costatum (Greville) Cleve, de Cananéia (25şS - 48şW), com especial referência ao fator salinidade. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 2v.

[2] AMINOT, A. & CHAUSSEPIED, M. 1983. Manuel des analyses chimiques en milieu marin. Brest, Centre National pour L'Exploitation des Oceans. 395 p.

[3] BEN-AMOTZ, A. & GILBOA, A. 1980. Cryopreservation of marine unicellular algae. II. Induction of freezing tolerance. Mar. Ecol.-Progr. Ser., 2:221-224.

[4] BRAARUD, T. 1961. Cultivation of marine organisms as a means of understanding environmental influences on populations. In: Sears, M., ed. Oceanography. Washington, DC : American Association for Advancement of Science Publication, 67:271- 298.

[5] COLLOS, Y. 1986. Time-lag algal growth dynamics: biological constraints on primary production in aquatic environments. Mar. Ecol.-Prog. Ser., 33:193-206.

[6] DROOP, M. R. 1958. Optimun relative and actual ionic concentrations for growth of some euryhaline algae. Verh. int. Ver. Limnol., 13:722-730.

[7] ______ 1975. The nutrient status of algal cells in batch culture. J. mar. biol. Ass. U.K., 55:541-555.

[8] DUBOIS, M.; GILLES, K. A.; HAMILTON, J. K.; REBERS, P. A. & SMITH, F. 1956. Colorimetric method for determination of sugars and related substances. Analyt. Chem., 18:350-356.

[9] FABREGAS, J.; ABALDE, J.; HERRERO, C; CABEZAS, B. & VEIGA, M. 1984. Growth of the marine microalga Tetraselmis suecica in batch cultures with different salinities and nutrient concentrations. Aquaculture, 42:207-215.

[10] FAWLEY, M. W. 1984. Effects of light intensity and temperature interactions on growth characteristics of Phaeodactylum tricomutum (Bacillariophyceae). J. Phycol., 20:67-72.

[11] GAETA, S. A. 1985. Comparação das respostas de crescimento e fotossíntese de três clones de Phaeodactylum tricornutum Bohlin. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 106p.

[12] ¹²
______ 1987. Efeitos do mercúrio (HgC₁₂) sobre o crescimento e fotossíntese de Phaeodactylum tricornutum Bohlin. Tese de doutorado. Universidade de São Paulo, Instituto Oceanográfico. 234 p.

[13] GOLDMAN, J. C. & GLIBERT, P. M. 1983. Kinetics of inorganic nitrogen uptake by phytoplankton. In: Carpenter, E. J. & Capone, D. G., eds Nitrogen in the marine environment. New York, Academic Press, p.233-274.

[14] GRASSHOFF, K.; EHRHARDT, M. & KREMLING, K. 1983. Methods of seawater analysis. 2nd revised and extended ed. Weinhein, Verlag Chemie. 419 p.

[15] GUILLARD, R. R. L. & RYTHER, J. H. 1962. Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol., 8:229-239.

[16] HARVEY, H. W. 1939. Substances controlling the growth of a diatom. J. mar. biol. Ass. U.K., 23:499-520.

[17] KAIN, J. M. & FOGG, G. E. 1958. Studies on the growth of marine phytoplankton. I. Asterionella japonica Gran. J. mar. biol. Ass. U.K., 37:397-413.

[18] LAING, I. 1985. Growth response of Chaetoceros calcitrans (Bacillariophyceae) in batch culture to a range of initial silica concentrations. Mar. Biol., 85:37-41.

[19] LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. 1951. Protein measurement with the folin phenol reagent. J. biol. Chem., 193:265-275.

[20] MYKLESTAD, S. & HAUG, A. 1972. Production of carbohydrates by the marine Chaetoceros affinis var. Willei (Gran.) Hustedt. I. Effect of the concentration of nutrients in the culture medium. J. exp mar. Biol. Ecol., 9:125-136.

[21] PRÉZELIN, B. B. 1981. Light reactions in photosynthesis. In: Platt, T. ed. Physiological bases of phytoplankton ecology.

[22] Can. Bull. Fish, aquat. Sci., (210):1-43.

[23] PRIBIL, S. & MARVAN, P. 1979. Batch cultivation. In: Marvan, P., Pribil, S. & Lhotsy, O. eds Algal assays and monitoring eutrophication. Stuttgart, E. Schweizerbart'sche Verlagsbuchhandlung (Ngele u. Obermiller), p.175-180.

[24] PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-428.

[25] RAIMBAULT, P. 1986. Effect of temperature on nitrite excretion by three marine diatoms during nitrate uptake. Mar. Biol., 92 (2):149-155.

[26] SASSI, R.; KUTNER, M. B. B. & MOURA, G. F. 1988. Studies on the decomposition of drift seaweed from the northeast Brazilian coastal reefs. Hydrobiologia, 157:187-192.

[27] SIGAUD, T. C. S. 1990. Efeitos de temperatura e salinidade sobre as respostas de crescimento e o conteúdo de clorofila-a de algumas espécies de algas planctónicas, em cultura. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 352 p.

[28] STEEMANN-NIELSEN, E. 1978. Principal aspects concerning the batch technique in algal assays. Mitt, int. Verein. Theor. angew. Limnol., 21:81-87.

[29] STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):p.1-311.

[30] VIEIRA, A. A. H. 1975. Estudos experimentais em fitoplâncton marinho. Culturas e aspectos ecofisiológicos. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico. 162 p.