Y-STR diversity and ethnic admixture in White and Mulatto Brazilian population samples

HUMAN AND MEDICAL GENETICS

SHORT COMMUNICATION

Y-STR diversity and ethnic admixture in White and Mulatto Brazilian population samples

Luzitano Brandão Ferreira; Celso Teixeira Mendes-Junior; Cláudia Emília Vieira Wiezel; Marcelo Rizzatti Luizon; Aguinaldo Luiz Simões

Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil

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ABSTRACT

We investigated 50 Mulatto and 120 White Brazilians for the Y-chromosome short tandem repeat (Y-STR) markers (DYS19, DYS390, DYS391, DYS392 and DYS393) and found 79 different haplotypes in the White and 35 in the Mulatto sample. Admixture estimates based on allele frequencies showed that the admixture of the white sample was 89% European, 6% African and 5% Amerindian while the Mulatto sample was 93% European and 7% African. Results were consistent with historical records of the directional mating between European males and Amerindian or African females.

Key words: Brazil, Mulatto, White, Y-STR.

The Brazilian population is a result of interethnic crosses of Europeans, Africans and Amerindians, and is one of the most heterogeneous populations in the world. When the first European colonizers arrived (1500 AD), 1-5 million Amerindians already lived in the region that now is known as Brazil (Salzano and Callegari-Jacques, 1988). Before 1820, European colonization was almost exclusively composed of Portuguese while between 1820 and 1975 the great majority of immigrants were from Portugal and Italy, followed by a small number by people from Spain, Germany, Syria and Japan (Carvalho-Silva et al., 2001). Between the 16^th and 19th centuries approximately 3.5 million Africans were brought as slaves to Brazil, coming mainly from West, West-Central and Southeast Africa (Curtin, 1969). The colonization of Brazil involved mostly European men, many of whom produced children with Amerindian and African females.

Although the classification of races is wrong from genetic standpoint (Templeton, 1998), Brazilians are classified for census purposes based on color. According to the last Brazilian government census of the 170 million Brazilians, 84 million were males, of which 52% were White, 39% were Brown, 6% were Black and 3% were classified in other categories (IBGE, 2000). Mulatto is the term commonly used in Brazil to designate the offspring result from the union of White and Black people. We used five Y-chromosome short tandem repeat (Y-STR) markers, recognized as good markers for population studies, to investigate genetic polymorphism and ethnic admixture in White and Mulatto Brazilian population samples.

We investigated 170 healthy, unrelated, individuals seeking paternity investigation at the Ribeirão Preto University Hospital, in the city of Ribeirão Preto, São Paulo state, Southeastern Brazil. The race of the individuals in the sample was determined based on their biomedical records, 120 individuals being White and 50 Mulatto, from Ribeirão Preto and the surrounding towns.

We assessed the Y-STR loci DYS19, DYS390, DYS391, DYS392 and DYS393 (Kayser et al., 1997) using blood samples taken from the individuals in our sample, DNA being extracted by an adaptation of the protocol of Higuchi (1989). The PCR reactions were performed according to the conditions described by Kayser et al. (1997) and products separated using 10% denaturing polyacrylamide gel electrophoresis and visualized by silver staining (Sanguinetti et al., 1994). Allele designations were determined by comparison of the sample fragments with those of control DNA samples previously cloned and sequenced.

Allele and haplotype frequencies were estimated by the gene counting method and gene and haplotype diversities calculated using the ARLEQUIN software version 2.000 (Schneider et al., 2000). Admixture proportions, based on allelic frequencies, were calculated by the gene identity method (Chakraborty, 1975) using the ADMIX3 program. Parental populations used in this study were from Portugal (González-Neira et al., 2000; Carvalho et al., 2003), Italy (Biondo et al., 1998), Guinea-Bissau (Rosa et al., 2006), Angola (Corte-Real et al., 2000), Mozambique (Pereira et al., 2002) and South American Indians (Rodriguez-Delfin et al, 1997; Tarazona-Santos et al, 2001). This study was approved by the Research Ethics Committee of the Ribeirão Preto University Hospital, Ribeirão Preto School of Medicine, University of São Paulo.

New alleles were not found, the frequencies of those found being shown in Table 1. A total of 79 (62 unique) different haplotypes were identified in the White and 35 (27 unique) in the Mulatto sample. The haplotype diversity was 0.9822 ± 0.0053 in the White and 0.9747 ± 0.0118 in the Mulatto sample. The most frequent haplotype 14-24-11-13-13 (DYS19-DYS390-DYS391-DYS392-DYS393) was encountered in 11 (9%) White and 6 (17%) of Mulatto individuals. The next most frequent haplotype (14-24-10-13-13) differed from the most frequent haplotype by only a single DYS391 repeat and was shared by 9 (7%) White and 5 (14%) Mulatto individuals. Together, the two most frequent haplotypes accounted for 26% of the White and 31% of the Mulatto sample.

The Mulatto population samples analyzed for paternal lineage in the present study showed 0.93 ± 0.01 of European and 0.07 ± 0.01 of African component (Table 2). The European contribution was different to that verified by HLA. polymorphisms in the State of Paraná, where the relative contribution was 0.42 ± 0.06 (Probst et al., 2000). As observed in Mulattos, previous studies with White Brazilian populations have demonstrated that the degree of admixture varied depending on the genetic marker used. While the Y-chromosome lineages in White samples were almost exclusively from European origin (Carvalho-Silva et al., 2001), mtDNA showed similar European, African and Amerindian contributions (Alves-Silva et al., 2000). In our study, the admixture estimate for the White sample was 0.89 ± 0.01 European, 0.06 ± 0.01 African and 0.05 ± 0.01 Amerindian. As shown in Table 2, the high European component is similar to that found in the Brazilian Y-chromosome study carried out by Carvalho-Silva et al. (2001) and in the study by Marrero et al. (2005) who investigated a population sample from the southernmost Brazilian state of Rio Grande do Sul which has a predominantly White population, but greater than that verified with HLA polymorphisms (Probst et al., 2000).

Ethnic admixture based on five Y-STR loci in the present study showed a high predominance of the European component in the White and Mulatto samples. These results are consistent with historical records of the directional mating between European males and Amerindian or African females during the colonization of Brazil.

Acknowledgments

Many thanks to Maria do Carmo Tomitão Canas and Ana Lúcia Pimentel for their excellent technical assistance. This work was supported by the Brazilian Agencies CNPq, CAPES and FAPESP, Brazil.

Instituto Brasileiro de Geografia e Estatística (IBGE), www.ibge. gov.br.

Received: September 26, 2005; Accepted: March 15, 2006.

Associate Editor: Francisco Mauro Salzano

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Publication Dates

• Publication in this collection
  21 Nov 2006
• Date of issue
  2006

History

• Received
  26 Sept 2005
• Accepted
  15 Mar 2006