Occurrence of Eulaema (Apeulaema) pseudocingulata Oliveira (Hymenoptera: Apidae: Euglossini) in the Platina Basin, Mato Grosso State, Brazil

Ocorrência de Eulaema (Apeulaema) pseudocingulata Oliveira (Hymenoptera: Apidae: Euglossini) na Bacia Platina, MT

SCIENTIFIC NOTE

Occurrence of Eulaema (Apeulaema) pseudocingulata Oliveira (Hymenoptera: Apidae: Euglossini) in the Platina Basin, Mato Grosso State, Brazil

Ocorrência de Eulaema (Apeulaema) pseudocingulata Oliveira (Hymenoptera: Apidae: Euglossini) na Bacia Platina, MT

Evandson J. dos Anjos-Silva

Depto. Biologia, UNEMAT Univ. Estado de Mato Grosso, C. postal 242, Cáceres, MT; Depto. Biologia, FFCLRP-USP – Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-USP, 14040-901, Ribeirão Preto, SP, [e-mail]

ABSTRACT

This study was conducted in the Chapada dos Guimarães National Park and in the Província Serrana of Mato Grosso state, from May 2003 to July 2005 and involved the use of chemical baits to attract male orchid bees. A total of 498 males were captured, of which 38 were Eulaema (Apeulaema) pseudocingulata Oliveira. Twenty-nine of these were collected in the national park and nine in the Província Serrana. E. pseudocingulata was reported as endemic to the Amazon Basin, however in the Platina Basin the species is sympatric to E. cingulata (Fabricius), a sibling species of similar morphology and color pattern but with a broader geographic distribution.

Key words: Apinae, orchid bee, geographic distribution, Eulaema cingulata

RESUMO

Em um estudo conduzido no Parque Nacional da Chapada dos Guimarães e na Província Serrana de Mato Grosso, iscas-odores foram utilizadas de maio de 2003 a julho de 2005 para atrair machos de Euglossini. Um total de 498 machos foi capturado, dos quais 38 eram machos de Eulaema (Apeulaema) pseudocingulata Oliveira. Foram capturados 29 machos no parque nacional e nove machos na Província Serrana. E. pseudocingulata foi recentemente descrita e, antes do presente trabalho, relatada como endêmica da Bacia Amazônica. Todavia, na Bacia Platina, ocorre em simpatria com E. cingulata (Fabricius), espécie similar na morfologia e no padrão de coloração, mas com ampla distribuição geográfica.

Palavras-chave: Apinae, abelha das orquídeas, distribuição geográfica, Eulaema cingulata

Brazil and Colombia report the highest diversity of orchid bee species in the world, with 117 and 116 species, respectively (Moure 2000, Roubik 2004, Ramírez 2005, Anjos-Silva & Rebêlo 2006), followed by Peru (79), Venezuela (70) and Panama (69) (Ramírez et al. 2002, Roubik & Hanson 2004, Rasmussen & Skov 2006, Oliveira 2006a). There are 208 valid species of euglossine bees presently known to man, 96% of which are free-living.

Of all the free-living genera, the genus Euglossa Latreille is the richest in number of species, represented by 113 species (Rebêlo & Moure 1995, Moure & Schlindwein 2002, Roubik 2004, Parra-H. et al. 2006) and ranging from Mexico to Argentina. Eufriesea Cockerell is the second largest genus of orchid bees, with over 60 described species that range from southern United States and Mexico to northern Argentina (Minckley & Reyes 1996, Búrquez 1997, Ramírez et al. 2002, Roubik & Hanson 2004).

The cleptoparasitic genus Exaerete Hoffmannsegg is represented by seven species, ranging from Mexico to southern Argentina (Anjos-Silva & Rebêlo 2006), and Aglae Lepeletier & Serville is a monotypic genus that was presumed to occur only in the Amazon Basin (Moure 1964, 1967; Michener 2000, Cameron 2004). However, the range of Aglae caerulea Lepeletier & Serville was recently documented in the Basin Platina, increasing its known geographic distribution by approximately 2,400 kilometers southward in South America (Anjos-Silva et al. 2006).

The genus Eulaema occurs from Rio Grande do Sul (Wittmann et al. 1988) (Brazil), Missiones (Argentina) and Paraguay to central Mexico (Moure 2000, Michener 2000, Cameron 2004, Oliveira 2006a). This genus was initially represented by 15 species (Moure 1950, Dressler 1979, Ospina-Torres 1998). Moure (2000) added some new species to the genus, including E. helvola Moure from the Brazilian states of Goiás and Mato Grosso, and E. basicincta Moure from Peru. Recently, Oliveira (2006b) described three new species endemic to the Amazon Basin: E. napensis Oliveira from Ecuador, E. parapolyzona Oliveira from Colombia and Bolivia, and E. pseudocingulata Oliveira, a new sibling species to E. cingulata (Fabricius). In his study, the author identifies 26 valid species of the genus Eulaema.

According to existing literature, the E. cingulata occurs from northwest Mexico to Bolivia and southern Brazil, whereas distribution of the E. pseudocingulata Oliveira is restricted to the Amazon Basin, Venezuela and Trinidad & Tobago. Both species belong to the subgenus Apeulaema Moure (Moure 1950, 2000), however E. pseudocingulata belongs to the polychroma group and E. cingulata to the nigrita group (Oliveira 2006a, b). Although several studies have been made in the Neotropical region, as yet none have documented whether the distributional gap within the polychroma and nigrita groups is a real phenomenon. The aim of this study is to record, for the first time, the presence of E. pseudocingulata outside the Amazon Basin.

The research was conducted in three study areas. The first is the gallery forest of the Véu de Noiva valley in the Chapada dos Guimarães National Park (15º24'21"S – 55º50'12"W), southern Mato Grosso state. The Planalto dos Guimarães is the natural boundary between the Amazon, Platina and Araguaia Basins and represents the extreme northeastern boundaries of the Upper Paraguay Basin, the altitude of which ranges from 300 m to 836 m, with annual rainfall ranging from 1,750 mm to 2,000 mm, and temperatures ranging from 13ºC to 30ºC (PCBAP 1997). The basement rock in the study area is entirely sandstone. The other two sites were located in the Serra do Quilombo, both located in the Província Serrana, southern Mato Grosso state. The basement rock in this area is limestone, forming a karstic landform comprised of sinkholes, sinking streams and cave passageways, commonly present in soluble rock such as limestone. The first of these sites is the Fazenda Jacobina (16º12'25"S – 57º34'30"W), and the second, the Água Milagrosa sinkhole (16º02'58"S – 57º31'49"W), with an altitude ranging from 600 to 390 m, an annual rainfall from 400 mm to 1,300 mm and temperatures between 15ºC and 35ºC (PCBAP 1997).

From May 2003 to July 2005, male euglossine bees were collected monthly with an insect collecting net as they arrived at the chemical baits. A total of eight chemical baits were used: benzyl benzoate, 1,8 - cineole, eugenol, vanillin, methyl acetate, methyl cinnamate, methyl salicylate and benzyl acetate. The baits were simultaneously applied to absorbent paper pads from 8:00 am to 4:00 pm, which were suspended from twigs by string 5 m apart and 1.5 m above the ground. The paper pads were replenished every 60 min with 1 ml of chemical to prevent loss due to their volatility. The males collected were placed in a killing jar containing ethyl acetate and then transferred to plastic vials. The specimens were collected as part of an ongoing project concerning the ecology and phylogeny of orchid bees. Voucher specimens of both E. pseudocingulata and E. cingulata were deposited in the collection of the Departamento de Biologia da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto-USP, Museu de Zoologia-USP, the entomological collection of the Instituto Nacional de Pesquisas da Amazônia-INPA, while the other specimens were stored in the author's collection.

In the first year, from May 2003 to August 2004 the baits attracted 392 males distributed among four genera and 24 valid species: Eulaema (3 species), Eufriesea (3 species), Euglossa (17 species) and Exaerete (1 species). A single male of E. pseudocingulata was collected at the Fazenda Jacobina in September 2003, while six males visited the baits during April, June, September, and December in Água Milagrosa. In the Chapada dos Guimarães National Park, 25 E. pseudocingulata males were collected at the baits from January to August 2004. The E. cingulata males (n = 2) visited the baits in January 2004 at the Fazenda Jacobina, and in the Chapada dos Guimarães the males (n = 11) visited the baits in October 2003 and from January to April 2004. The E. cingulata males (n = 13) and E. pseudocingulata males (n = 32) were attracted to eugenol, vanillin, 1,8 cineole and benzyl de benzoate.

In the second year, from May 2004 to July 2005 the baits attracted 106 males distributed among five genera and 13 valid species: Aglae (1 species), Eulaema (5 species), Eufriesea (1 species), Euglossa (4 species) and Exaerete (2 species). At the Fazenda Jacobina only males of the Eulaema and Exaerete genera were collected. The E. cingulata males (n = 4) visited the baits in February and April 2005. In Água Milagrosa 14 males belonging to five valid species (and three undescribed species) and four genera were collected: Eulaema (3 species), Eufriesea (2 species), Euglossa (1 species), and Exaerete (2 species). E. pseudocingulata males (n = 2) visited the baits in January and March 2005, and E. cingulata males (n = 1) visited the baits in February 2005. In the Chapada dos Guimarães 87 males belonging to 12 valid species (and to several additional, undescribed species) and five genera were collected: Aglae (1 species), Eulaema (4 species), Eufriesea (1 species), Euglossa (4 species), Exaerete (2 species). A total of 16 males belonged to the genus Eulaema. The E. cingulata males (n = 9) were collected in October and December 2004 and in January, February, May and July 2005. The E. pseudocingulata males (n = 4) were collected in September 2004 and January and July 2005. The E. cingulata males (n = 14) and the E. pseudocingulata males (n = 6) were attracted only to benzyl acetate in the second year (Anjos-Silva 2006a).

Oliveira (2006a, b) suggested that the geographical distribution of the E. pseudocingulata is restricted to the Amazon Basin, however recent records of the species (this paper) have modified that distribution. The results reveal that (i) the geographical distribution pattern of E. pseudocingulata is not Guianan-Amazonian as previously maintained by Oliveira (2006a, b); (ii) the data now available (Anjos-Silva 2006b) suggest that their distribution areas even overlap those of E. cingulata; (iii) E. pseudocingulata has a wider distribution than Oliveira (2006a, b) previously reported; (iv) E. pseudocingulata is not a rare species, on the contrary, it is a very common species in the Platina Basin and is more abundant than its sibling E. cingulata; (v) this gap is a consequence of the poor knowledge on the cerrado euglossine fauna. For example, the distribution of E. pseudocingulata may be larger than presently understood owing to poor sampling in the cerrado and pantanal wetlands (Anjos-Silva 2006b). These two biotas constitute geographical gaps and the distribution of E. pseudocingulata may eventually be discovered to extend further southward (e.g., southern Brazilian forests). Nonetheless, further biological studies on the species will either confirm or refute this suspicion.

Before the present study, the presence of E. pseudocingulata had not been reported in the Platina Basin, where this species occurs in sympatry with E. cingulata, a species described by Fabricius in the early XIX century. Therefore, although the presence of E. pseudocingulata in the Chapada dos Guimarães National Park and Província Serrana of Mato Grosso may be seen as surprising, its occurrence there represents just one more example of the Amazon forest's influence in the composition of the Cerrado domain fauna.

Acknowledgments

I am grateful to Evandro Camillo and Carlos Alberto Garófalo for their support. Two anonymous referees reviewed the manuscript providing useful comments. Support was provided by a CAPES research grant, PQI 0053/02-3, while CNPq EU 473857/03-0 generously supported the work in the FFCLRP/USP.

References

Anjos-Silva, E.J. dos. 2006a. Fenologia das abelhas Euglossini Latreille (Hymenoptera: Apidae) e a variação sazonal e geográfica na escolha e preferência por iscas-odores no Parque Nacional de Chapada dos Guimarães e na Província Serrana de Mato Grosso, Brasil. Tese de doutorado, FFCLRP/USP, Ribeirão Preto, 114p.

Anjos-Silva, E.J. dos. 2006b. Orchid bee species from Mato Grosso: An appraisal. Anais do VII Encontro sobre Abelhas, p.503-509 (CD-Rom), Ribeirão Preto, USP, 12-15 de julho.

Anjos-Silva, E.J. dos, E. Camillo & C.A. Garófalo. 2006. Occurrence of Aglae caerulea Lepeletier & Serville (Hymenoptera: Apidae: Euglossini) in the Chapada dos Guimarães National Park, Mato Grosso state, Brazil. Neotrop. Entomol. 35: 868-870.

Anjos-Silva, E.J. dos & J.M.M. Rebêlo. 2006. A new species of Exaerete Hoffmannsegg (Hymenoptera: Apidae: Euglossini) from Brazil. Zootaxa 1105: 27-35.

Búrquez, A. 1997. Distributional limits of euglossine and meliponine bees (Hymenoptera: Apidae) in northwestern Mexico. Pan-Pac. Entomol. 73: 137-140.

Cameron, S.A. 2004. Phylogeny and biology of Neotropical orchid bees (Euglossini). Annu. Rev. Entomol. 49: 377-404.

Dressler, R.L. 1979. Eulaema bombiformis, E. meriana and mullerian mimicry in related species (Hymenoptera: Apidae). Biotropica 11: 144-151.

Michener, C.D. 2000. The bees of the world. Baltimore, Johns Hopkins Univ. Press, xiv + 913p.

Minckley, R.L. & S.G. Reyes. 1996. Capture of orchid bee, Eulaema polychroma (Friese) (Apidae: Euglossini) in Arizona, with notes on northern distributions of other Mesoamerican bees. J. Kans. Entomol. Soc. 69: 102-104.

Moure, J.S. 1950. Contribuição para o conhecimento do gênero Eulaema Lepeletier. Dusenia 1: 181-200.

Moure, J.S. 1964. A key to the parasitic Euglossinae bees and a new species of Exaerete from Mexico (Hymenoptera-Apoidea). Rev. Biol. Trop. 15: 227-247.

Moure, J.S. 1967. A check-list of the know euglossine bees (Hymenoptera, Apidae). Atas Simp. Biota Amazôn. 5: 395-415.

Moure, J.S. 2000. As espécies do gênero Eulaema Lepeletier, 1841 (Hymenoptera, Apidae, Euglossinae). Acta Biol. Parana. 29: 1-70.

Moure, J.S. & C. Schlindwein. 2002. Uma nova espécie de Euglossa (Euglossella) Moure do Nordeste do Brasil (Hymenoptera, Apidae). Rev. Bras. Zool. 19: 585-588.

Oliveira, M.L. 2006a. Nova hipótese de relacionamento filogenético entre os gêneros de Euglossini e entre as espécies de Eulaema Lepeletier, 1841 (Hymenoptera: Apidae: Euglossini). Acta Amazôn. 36: 273 - 286.

Oliveira, M.L. 2006b. Três novas espécies de abelhas da Amazônia pertencentes ao gênero Eulaema (Hymenoptera: Apidae: Euglossini). Acta Amazôn. 36: 121-128.

Ospina-Torres, R. 1998. Revisión de la morfología genital masculina de Eulaema (Hymenoptera: Apidae). Rev. Biol. Trop. 40: 749-762.

Parra-H., A., R. Ospina-Torres & S. Ramírez. 2006. Euglossa natesi n. sp., a new species of orchid bee from the Chocó region of Colombia and Ecuador (Hymenoptera: Apidae). Zootaxa 1298: 29-36.

PCBAP. 1997. Plano de conservação da bacia do Alto Paraguai. Diagnóstico dos meios físico e biótico – Meio físico. Vol. II, Tomo I. Brasília, MMA, 334p. Ramírez, S. 2005. Euglossa paisa, a new species of orchid bee from the Colombian Andes (Hymenoptera: Apidae). Zootaxa 1065: 51-60.

Ramírez, S. 2005. Euglossa paisa, a new species of orchid bee from the Colombian Andes (Hymenoptera: Apidae). Zootaxa 1065: 51-60.

Ramírez, S., R.L. Dressler & M. Ospina. 2002. Abejas euglossinas (Hymenoptera: Apidae) de la región Neotropical: Lista de especies con notas sobre su biología. Biota Colombiana 3: 7-118.

Rasmussen, C. & C. Skov. 2006. Description of a new species of Euglossa (Hymenoptera: Apidae: Euglossini) with notes on comparative biology. Zootaxa 1210: 53-67.

Rebêlo, J.M.M. & J.S. Moure. 1995. As espécies de Euglossa Latreille do nordeste do Estado de São Paulo (Apidae, Euglossinae). Rev. Bras. Zool. 12: 445-466.

Roubik, D.W. 2004. Sibling species of Glossura and Glossuropoda in the Amazon Region (Hymenoptera: Apidae: Euglossini). J. Kans. Entomol. Soc. 77: 235-253.

Roubik, D.W. & P.E. Hanson. 2004. Orchid bees from Tropical America. Biology and field guide. INBio Press, Santo Domingo de Heredia, 352p.

Wittmann, D., M. Hoffmann & E. Scholz. 1988. Southern distributional limits of euglossine bees in Brazil linked to habitats of the Atlantic- and subtropical rain forest (Hymenoptera: Apidae: Euglossini). Entomol. Gen. 14: 53-60.

Received 02/VIII/06. Accepted 19/XII/06.

References

• Anjos-Silva, E.J. dos. 2006a. Fenologia das abelhas Euglossini Latreille (Hymenoptera: Apidae) e a variação sazonal e geográfica na escolha e preferência por iscas-odores no Parque Nacional de Chapada dos Guimarães e na Província Serrana de Mato Grosso, Brasil. Tese de doutorado, FFCLRP/USP, Ribeirão Preto, 114p.
• Anjos-Silva, E.J. dos. 2006b. Orchid bee species from Mato Grosso: An appraisal. Anais do VII Encontro sobre Abelhas, p.503-509 (CD-Rom), Ribeirão Preto, USP, 12-15 de julho.
• Anjos-Silva, E.J. dos, E. Camillo & C.A. Garófalo. 2006. Occurrence of Aglae caerulea Lepeletier & Serville (Hymenoptera: Apidae: Euglossini) in the Chapada dos Guimarães National Park, Mato Grosso state, Brazil. Neotrop. Entomol. 35: 868-870.
• Anjos-Silva, E.J. dos & J.M.M. Rebêlo. 2006. A new species of Exaerete Hoffmannsegg (Hymenoptera: Apidae: Euglossini) from Brazil. Zootaxa 1105: 27-35.
• Búrquez, A. 1997. Distributional limits of euglossine and meliponine bees (Hymenoptera: Apidae) in northwestern Mexico. Pan-Pac. Entomol. 73: 137-140.
• Cameron, S.A. 2004. Phylogeny and biology of Neotropical orchid bees (Euglossini). Annu. Rev. Entomol. 49: 377-404.
• Dressler, R.L. 1979. Eulaema bombiformis, E. meriana and mullerian mimicry in related species (Hymenoptera: Apidae). Biotropica 11: 144-151.
• Michener, C.D. 2000. The bees of the world. Baltimore, Johns Hopkins Univ. Press, xiv + 913p.
• Minckley, R.L. & S.G. Reyes. 1996. Capture of orchid bee, Eulaema polychroma (Friese) (Apidae: Euglossini) in Arizona, with notes on northern distributions of other Mesoamerican bees. J. Kans. Entomol. Soc. 69: 102-104.
• Moure, J.S. 1950. Contribuição para o conhecimento do gênero Eulaema Lepeletier. Dusenia 1: 181-200.
• Moure, J.S. 1964. A key to the parasitic Euglossinae bees and a new species of Exaerete from Mexico (Hymenoptera-Apoidea). Rev. Biol. Trop. 15: 227-247.
• Moure, J.S. 1967. A check-list of the know euglossine bees (Hymenoptera, Apidae). Atas Simp. Biota Amazôn. 5: 395-415.
• Moure, J.S. 2000. As espécies do gênero Eulaema Lepeletier, 1841 (Hymenoptera, Apidae, Euglossinae). Acta Biol. Parana. 29: 1-70.
• Moure, J.S. & C. Schlindwein. 2002. Uma nova espécie de Euglossa (Euglossella) Moure do Nordeste do Brasil (Hymenoptera, Apidae). Rev. Bras. Zool. 19: 585-588.
• Oliveira, M.L. 2006a. Nova hipótese de relacionamento filogenético entre os gêneros de Euglossini e entre as espécies de Eulaema Lepeletier, 1841 (Hymenoptera: Apidae: Euglossini). Acta Amazôn. 36: 273 - 286.
• Oliveira, M.L. 2006b. Três novas espécies de abelhas da Amazônia pertencentes ao gênero Eulaema (Hymenoptera: Apidae: Euglossini). Acta Amazôn. 36: 121-128.
• Ospina-Torres, R. 1998. Revisión de la morfología genital masculina de Eulaema (Hymenoptera: Apidae). Rev. Biol. Trop. 40: 749-762.
• Parra-H., A., R. Ospina-Torres & S. Ramírez. 2006. Euglossa natesi n. sp., a new species of orchid bee from the Chocó region of Colombia and Ecuador (Hymenoptera: Apidae). Zootaxa 1298: 29-36.
• PCBAP. 1997. Plano de conservação da bacia do Alto Paraguai. Diagnóstico dos meios físico e biótico – Meio físico. Vol. II, Tomo I. Brasília, MMA, 334p.
• Ramírez, S. 2005. Euglossa paisa, a new species of orchid bee from the Colombian Andes (Hymenoptera: Apidae). Zootaxa 1065: 51-60.
• Ramírez, S. 2005. Euglossa paisa, a new species of orchid bee from the Colombian Andes (Hymenoptera: Apidae). Zootaxa 1065: 51-60.
• Ramírez, S., R.L. Dressler & M. Ospina. 2002. Abejas euglossinas (Hymenoptera: Apidae) de la región Neotropical: Lista de especies con notas sobre su biología. Biota Colombiana 3: 7-118.
• Rasmussen, C. & C. Skov. 2006. Description of a new species of Euglossa (Hymenoptera: Apidae: Euglossini) with notes on comparative biology. Zootaxa 1210: 53-67.
• Rebêlo, J.M.M. & J.S. Moure. 1995. As espécies de Euglossa Latreille do nordeste do Estado de São Paulo (Apidae, Euglossinae). Rev. Bras. Zool. 12: 445-466.
• Roubik, D.W. 2004. Sibling species of Glossura and Glossuropoda in the Amazon Region (Hymenoptera: Apidae: Euglossini). J. Kans. Entomol. Soc. 77: 235-253.
• Roubik, D.W. & P.E. Hanson. 2004. Orchid bees from Tropical America. Biology and field guide. INBio Press, Santo Domingo de Heredia, 352p.
• Wittmann, D., M. Hoffmann & E. Scholz. 1988. Southern distributional limits of euglossine bees in Brazil linked to habitats of the Atlantic- and subtropical rain forest (Hymenoptera: Apidae: Euglossini). Entomol. Gen. 14: 53-60.

Publication Dates

• Publication in this collection
  09 Aug 2007
• Date of issue
  June 2007

History

• Received
  02 Aug 2006
• Accepted
  19 Dec 2006