Production of reactive oxygen species in Dalbergia nigra seeds under thermal stress

Produção de espécies reativas de oxigênio em sementes de Dalbergia nigra sob estresse térmico

Abstracts

Seed germination is dependent on abiotic factors, temperature being one of the main ones, whose influence causes seed damage under extreme conditions. The aim of the present study was to investigate the effect of different temperatures during germination of D. nigra seeds and their physiological and biochemical implications. We assessed germination percentage and production of superoxide anion (O2-) and hydrogen peroxide (H2O2) in seeds subjected to temperatures of 5, 15, 25, 35 and 45 ºC for different periods of time. Hydration is promoted at 45 ºC and inhibited at 5ºC, without germination in either, whereas it is minimal at 15 °C and at a maximum level at 25 °C. Superoxide production increases at higher temperatures (25 and 35 ºC) after 72 hours of hydration, coinciding with the beginning of radicle protrusion. Production of hydrogen peroxide decreases at all temperatures, except for 5 ºC, with values near each other at temperatures of 15, 25, and 35 ºC, where there was radicle protrusion.

forest; physiology; biochemistry; rosewood

A germinação de sementes é dependente de fatores abióticos, sendo a temperatura um dos principais, cuja influência, em condições extremas, causa danos às sementes. O presente trabalho teve por objetivo investigar o efeito das diferentes temperaturas durante a germinação de sementes de D. nigra e suas implicações fisiológicas e bioquímicas. Avaliaram-se o porcentual de germinação e a produção de ânion superóxido (O2-) e peróxido de hidrogênio (H2O2) em sementes submetidas às temperaturas de 5, 15, 25, 35 e 45 ºC por diferentes tempos. A hidratação é estimulada a 45 ºC e inibida a 5 ºC, não havendo germinação em ambas, enquanto é mínima a 15 ºC e máxima a 25 ºC. A produção de superóxido aumenta nas temperaturas mais altas (25 e 35 ºC) após 72 horas de hidratação, coincidindo com o início da protrusão radicular. A produção de peróxido de hidrogênio decresce em todas as temperaturas, à exceção da de 5 ºC, com valores próximos entre si nas temperaturas de 15, 25 e 35 ºC, onde houve protrusão radicular.

floresta; fisiologia; bioquímica; jacarandá-da-bahia

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Publication Dates

• Publication in this collection
  09 Sept 2014
• Date of issue
  Sept 2014

History

• Accepted
  26 May 2014
• Received
  25 Feb 2014