Mycobacterium leprae and Mycobacterium lepromatosis in small mammals in Midwest Brazil

Abstract

Leprosy is a chronic infectious disease caused by the bacilli Mycobacterium leprae and Mycobacterium lepromatosis. In addition to humans, animals such as nine-banded armadillos and red squirrels are species naturally infected. The objective of this study was to investigate the presence of M. leprae and M. lepromatosis in non-volant small mammals of the order Didelphimorphia and Rodentia through Polymerase Chain Reaction (PCR) assay. During 2015 and 2018, field expeditions were carried out in three municipalities, covering biotic elements of the Amazon and Cerrado biomes, in the Mato Grosso State, Midwest of Brazil. A specific primer for repetitive sequences of the genomic DNA of M. leprae and M. lepromatosis targeting the RLEP and RLPM gene, respectively, was used to screen for these agents. The molecular detection of M. leprae DNA in the samples was 13.8%. M. lepromatosis was not detected. The present study reports a description of M. leprae in small non-volant mammals in Brazil.

Keywords
RLEP; RLPM; Amazonia; Cerrado

Leprosy, also known as hanseniasis or Hansen's disease, is an infectious disease caused by the bacterial pathogens Mycobacterium leprae and Mycobacterium lepromatosis.¹ It occurs mainly in tropical and subtropical countries and is considered a neglected tropical disease.²

Although M. leprae is considered a human-only pathogen, evidence of zoonotic cases has emerged after exposure to nine-banded armadillos (Dasypus novemcinctus) in the Americas.³ The transmission mechanisms between animals and humans are not well established; however, the probable route is aerosol transmission.⁴ Some studies have also reported the survival of this microorganism in the environment, amoebas and arthropods, which may contribute to the maintenance of the disease.⁵,⁶

As for M. lepromatosis, the global extent of infection and its effect on the development of hanseniasis are still unknown.⁷ This pathogen has already been reported in humans in Brazil, Mexico, the United States, and Southeast Asia.⁸ Recent studies have indicated that both M. leprae and M. lepromatosis may be involved in the development of leprosy.⁹

Countless questions about leprosy remain regarding its transmission and ecology, as well as its zoonotic and sapronotic reservoirs. Thus, the objective of this study was to investigate the presence of M. leprae and M. lepromatosis infections in non-volant small mammals of the orders Didelphimorphia and Rodentia in Brazil.

During 2014 and 2018, field expeditions were conducted in the municipalities of Alta Floresta (09°58′S, 56°04′W) and Sinop (11°49′S, 55°24′W), encompassing biotic elements from the Amazon, and Barra do Garças (15°54′S, 52°16′W), encompassing the Cerrado biome in Mato Grosso State in Midwestern Brazil. Small non-volant mammals were captured using wire cage traps and Sherman-like traps. The animals were sampled primarily for another project investigating the epidemiology of tick-borne diseases in small mammals¹⁰,¹¹ and the stored samples were made available for the present study. The captured mammals were anesthetized by intramuscular injection of ketamine hydrochloride/xylazine solution. All captured mammals were euthanized by increasing the anesthetic dose.

DNA extraction from 10 mg of spleen tissue was performed and PCR was used to screen the M. leprae using specific primers (R1 5′-CGC CCG GAT CCT CGA TGC AC-3 ' and R2 5′-GCA CGT AAG CTT GTC GGT GG-3′) targeting a fragment of a 372-base pair repetitive sequence corresponding to the RLEP gene region.¹² The expected amplicon sizes were purified and prepared for sanger sequencing. The obtained sequences were then queried using the Basic Local Alignment Search Tool (BLAST) to determine the closest identities with congeneric organisms available in GenBank. For the detection of M. lepromatosis, samples positive for the RLEP region were prepared using the TaqMan Universal PCR Master Mix (Applied Biosystems) with a TaqMan probe (5′-AAGTGACGCGGGCGTGGATT-3′) and specific primers (5′-TTGGTGATCGGGGTCGGCTGGA-3′; 5′-CCCCACCGGACACCACCAACC-3′) to amplify the RLPM region.¹³

Procedures in this study were approved by the Ethics Committee on Animal Research of the Federal University of Mato Grosso (CEUA protocol n° 23108.076870/2015-41) and “Instituto Chico Mendes de Conservação da Biodiversidade” (ICMBio permit n° 8863-1).

A total of 269 small non-volant mammals were surveyed for the presence of M. leprae. Overall, M. leprae was detected in 37 (13.8%) of 269 spleen samples, as evaluated using the RLEP PCR assay. Table 1 shows the detection of M. leprae in the small mammals. The DNA of a specimen of Didelphis albiventris, Cerradomys sp., Neacomys sp. and Thrichomys pachyurus was sequenced, obtaining from 99% to 100% of identity to the corresponding sequences of M. leprae (MF975706.1, MF975705.1) available on GenBank (Table 2). In the Amazon biome, three animals (1.1%) were positive for M. leprae infection, while in the Cerrado biome the positivity rate was 43.0% (n = 34). M. lepromatosis was not detected in any of the samples tested using the RLPM RT-PCR assay.

Table 1
Detection of Mycobacterium leprae in samples of marsupials and rodents in the state of Mato Grosso, Brazil.

Municipalities	Mammal species (n)	Mycobacterium leprae detected
		N° tested/N° positive (%)
Alta Floresta	Order didelphimorphia
	Caluromys philander (1)	1/0 (0)
	Cryptonanus sp. (9)	9/1 (11.1)
	Didelphis marsupialis (2)	2/0 (0)
	Glironia venusta (1)	1/0 (0)
	Marmosa constantiae (9)	9/1 (11.1)
	Marmosa murina (1)	1/0 (0)
	Marmosops bishop (7)	7/0 (0)
	Marmosops aff. pinheroi (3)	3/0 (0)
	Monodelphis glirine (3)	3/0 (0)
	Monodelphis saci (2)	2/0 (0)
	Order rodentia
	Hylaeamys megacephalu (3)	3/0 (0)
	Neacomys amoenus (5)	5/0 (0)
	Necromys Lasiurus (2)	2/0 (0)
	Oecomys cleberi (2)	2/0 (0)
	Oecomys paricola (2)	2/0 (0)
	Oecomys aff. catherinae (1)	1/0 (0)
	Oligoryzomys cf (2)	2/0 (0)
	Proechimys sp. (4)	4/0 (0)
Sinop	Order didelphimorphia
	Caluromys philander (5)	5/0 (0)
	Didelphis marsupials (28)	28/0 (0)
	Gracilinanus peruanus (3)	3/0 (0)
	Marmosa constantiae (49)	49/0 (0)
	Marmosa murina (3)	3/0 (0)
	Metachirus nudicaudatus (4)	4/0 (0)
	Order rodentia
	Mesomys hispidus (3)	3/0 (0)
	Mus musculus (1)	1/0 (0)
	Oecomys bicolor (19)	19/0 (0)
	Oecomys paricola (1)	1/0 (0)
	Oecomys roberti (4)	4/0 (0)
	Proechimys roberti (11)	11/1 (9.0)
Barra do Garças	Order didelphimorphia
	Didelphis albiventris (6)	6/3 (50)
	Gracilinanus agilis (1)	1/1 (100)
	Monodelphis domestica (1)	1/0 (0)
	Order rodentia
	Cerradomys sp. (3)	3/1 (33.3)
	Neacomys sp. (1)	1/1 (100)
	Oecomys sp. (2)	2/0 (0)
	Thrichomys pachyurus (65)	65/28 (43.1)

Table 2
Samples from rodents and marsupials positive for Mycobacterium leprae sent for sequencing.

Municipality	Species (n° of samples)	GenBank Homology (%)
Barra do Garças	Order didelphimorphia
	Didelphis albiventris (1)	271/273pb (99)
	Order rodentia
	Cerradomys sp. (1)	247/249pb (99)
	Neacomys sp. (1)	238/238pb (100)
	Thrichomys pachyurus (1)	242/242pb (100)

This study is one of the few to report the presence of M. leprae infection in small non-volant mammals in Brazil. The organ of choice was the spleen because previous studies with experimentally infected armadillos have demonstrated the spleen with high rates of recovery from bacilli, with yields about 4 to 10 times higher than that of the liver.¹⁴

The state of Mato Grosso is a historic leprosy endemic area and the sustained occurrence of leprosy patients at hyperendemic levels (> 65/100,000 inhabitants)¹⁵ in most municipalities of the state may, in part, be associated with operational improvements in health services, including better coverage and decentralization.

The Cerrado (savanna) biome has been a global biodiversity hotspot with high rates of native vegetation suppression and wildfires over the past three decades. The samples collected in the Cerrado biome were located in the Serra Azul State Park, created in 1994 aiming at its environmental conservation. The area was previously occupied by indigenous groups and after colonization there was an intense occupation by miners, demonstrating an ancient anthropic impact in the region.¹⁶ Currently visitation to the Park is constant by residents and tourists. Considering that a person with multibacillary leprosy eliminates an estimate of 107 bacilli per day through nasal secretions,¹⁷ the survival of the agent in the environment and the high rates of leprosy in the state of Mato Grosso, the historical anthropic impact in the region that remains can favor the maintenance of the microorganism in the environment and consequent infection of the individuals who live there.

Worldwide, the detection of animals infected with M. leprae is still low; however, several groups have reported the possibility of reservoirs in wildlife, including in non-human primates, margay (Leopardus wiedii), and lowland tapirs (Tapirus terrestris),¹⁸ armadillos³ and red squirrels (Sciurus vulgaris).¹⁹ Thus, a role for animals in the persistence and transmission of M. leprae is increasingly cited as a real possibility.⁸

No M. lepromatosis was detected in any of the tested samples. Although studies have reported the presence of this agent in red squirrels in Europe,¹⁹ there is little research on its presence in leprosy-endemic countries. In a study by Schilling et al.,²⁰ M. lepromatosis was not detected in rodent samples from Mexico, which is similar to the findings of the present study.

The discovery of M. leprae in small non-volant mammals and its potential relationship with high leprosy rates in Mato Grosso, Brazil, is intriguing; however, it is important to note that to accurately estimate the risk presented by non-human reservoirs in transmitting this disease, more research is needed to identify additional leprosy reservoirs. This knowledge is crucial for developing better strategies for controlling the spread of these microorganisms in the future.

Ethics approval

Procedures in this study were previously approved by the Ethics Committee on Animal Research of the Federal University of Mato Grosso (CEUA protocol n° 23108.076870/2015-41) and “Instituto Chico Mendes de Conservação da Biodiversidade” (ICMBio permit n° 8863-1). All legal requirements and guidelines in Brazil for the care and use of animals have been followed.

Acknowledgment

The authors are grateful to CAPES for financial support through a scholarship.

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Publication Dates

• Publication in this collection
  04 Nov 2024
• Date of issue
  2024

History

• Received
  8 Mar 2024
• Accepted
  8 Sept 2024
• Published
  24 Sept 2024