Linalool chemotype essential oil from Lippia alba in the anesthesia of fat snook (Centropomus parallelus): ventilatory rate, biochemical, antioxidant, and oxidative status parameters

Abstract

This study evaluated the anesthetic activity of essential oil from Lippia alba (EOLA), linalool chemotype in a euryhaline fish (fat snook Centropomus parallelus). In the first experiment, fish were exposed to 30, 80, 130, 180, 200, and 230 µL EOLA L−1. The second experiment evaluated smaller and larger fish with 180 µL EOLA L-1. In the third experiment, ventilatory rates (VR) for up to 120 min in animals during exposure to 5 and 10 µL EOLA L−1 were evaluated. In the fourth experiment, fish anesthetized with 30 and 180 µL EOLA L-1 were assessed at 0, 30, and 60 min after anesthesia recovery to evaluate biochemical and antioxidant parameters. The best mild and deep anesthesia times were obtained with 30 and 180 µL EOLA L-1, and larger fish had the highest times. The VR increased in fish exposed to EOLA. Blood glucose and whole-body cortisol levels were higher in fish anesthetized with 180 µL EOLA L-1. Fish exposed to EOLA had higher liver glutathione S-transferase and superoxide dismutase activities without affecting catalase and lipid peroxidation levels. The 180 µL EOLA L-1 is recommended for fat snook anesthesia because it increases VR, blood glucose, and whole-body cortisol levels and prevents oxidative stress.

Keywords:
Anesthesia recovery; Cortisol; Glucose; Lipid peroxidation; Ventilatory rate

Resumo

Este estudo avaliou a atividade anestésica do óleo essencial de Lippia alba (OELA), quimiotipo linalool, em um peixe eurialino (robalo-peva Centropomus parallelus). No primeiro experimento, peixes foram expostos a 30, 80, 130, 180, 200 e 230 µL OELA L−1. O segundo experimento avaliou peixes pequenos e grandes com 180 µL OELA L-1. No terceiro experimento, avaliou-se taxa ventilatória por até 120 min em animais expostos a 5 e 10 µL OELA L−1. No quarto experimento, peixes anestesiados com 30 e 180 µL OELA L-1 foram avaliados nos tempos 0, 30 e 60 min após a recuperação anestésica para verificação de parâmetros bioquímicos e antioxidantes. Os melhores tempos de anestesia leve e profunda foram obtidos com 30 e 180 µL OELA L-1. Peixes maiores apresentaram anestesia e tempos de recuperação mais elevados. A taxa ventilatória aumentou em peixes expostos para OELA comparados ao grupo controle. Os níveis de glicose sanguínea e cortisol corporal foram maiores em peixes anestesiados com 180 µL OELA L-1. Robalos-peva expostos para OELA tiveram maior atividade de glutationa S-transferase e superóxido dismutase no fígado, sem afetar os níveis de catalase e peroxidação lipídica. O uso de 180 µL OELA L-1 é recomendado para anestesia de robalo-peva, pois aumentou taxa ventilatória e níveis de glicose sanguínea, cortisol corporal, e preveniu estresse oxidativo.

Palavras chave:
Cortisol; Glicose; Peroxidação lipídica; Recuperação anestésica; Taxa de ventilação

INTRODUCTION

The use of anesthetics in fish has been encouraged to minimize stress-inducing factors, such as hypermobility and perception of adverse stimuli during fish management (Teixeira et al., 2018; Oliveira et al., 2019a,b). Anesthetics can cause the inhibition of the respiratory center in the medulla oblongata, resulting in depression of the central nervous system (CNS) and decreasing the ventilatory rate (VR) (Ross, Ross, 2009). However, some anesthetics, mainly those of synthetic origin, depending on concentration and time of exposure, can trigger stress (Parodi et al., 2014; Teixeira et al., 2017) and induce undesirable collateral effects on metabolism or gill damage in fish (Kiessling et al., 2009; Wosnick et al., 2018; Oliveira et al., 2022), arousing interest in the investigation of anesthetic compounds originating from plants.

Cortisol indicates primary stress in fish and affects secondary stress biomarkers, like blood glucose levels, which increase in response to cortisol levels (Wendelaar Bonga, 1997). Commonly, circulating cortisol levels are typically measured in fish (Sena et al., 2016). The whole-body cortisol is an alternative that can detect distinct cortisol levels and measure the physiological stress response (Sink et al., 2007) when blood volumes are insufficient to provide measurements of circulating cortisol (Baldisserotto et al., 2014; Parodi et al., 2014). A more severe consequence of increased primary and secondary stress responses is the occurrence of problems in fish development, reproduction, health, and behavior, which are part of tertiary stress responses (Lemos et al., 2018).

Oxidative stress parameters are also considered critical stress indicators in fish (Chowdhury, Saikia, 2020). Oxidative stress can increase the reactive oxygen species (ROS) levels, affecting the maintenance of the cellular redox balance, i.e., cellular homeostasis and its regulation (Lushchak, 2011). In this sense, fish have enzymatic antioxidant defense systems (e.g., glutathione-S-transferase – GST, catalase – CAT, and superoxide dismutase – SOD) that can remove excessive damaging ROS, thus reducing the damage by lipid peroxidation (LPO), playing a critical role in the self-defense system of the body (Sies, 1997; Souza et al., 2018; Copatti et al., 2019).

Lippia alba (Mill.) N. E. Brown (Verbenaceae) is a native bush of South America with several chemotypes, many aromatic and medicinal properties, and low toxicity (Azambuja et al., 2011). The essential oil from L. alba (EOLA), linalool chemotype, has shown sedative and anesthetic effects in previous studies with several freshwater fish, such as silver catfish Rhamdia quelen (Quoy & Gaimard, 1824) (Cunha et al., 2010; Heldwein et al., 2014), tambacu (Piaractus mesopotamicus × Colossoma macropomum) (Sena et al., 2016), Nile tilapia Oreochromis niloticus (Linnaeus, 1758) (Hohlenwerger et al., 2016, 2017), and tambaqui Colossoma macropomum (Cuvier, 1816) (Batista et al., 2018), and a few marine species, as sea horse Hippocampus reidi Ginsburg, 1933 (Cunha et al., 2011), gilthead sea bream Sparus aurata Linnaeus, 1758 (Toni et al., 2015), and meagre Argyrosomus regius (Asso y del Rio, 1801) (Cárdenas et al., 2016). However, anesthetics can exhibit different efficiencies in fish that can move from salt to freshwater and vice versa (Sepulchro et al., 2016). However, its effects on these euryhaline fish are still little known.

Fat snook (Centropomus parallelus Poey, 1860) is a euryhaline fish species from the family Centropomidae that inhabits a wide range of salinities in marine and estuarine waters of the western coast of the Atlantic Ocean, from southern USA (State of Florida) to southern Brazil (State of Santa Catarina) (Tsuzuki et al., 2007). It is an opportunistic carnivore, appreciated by consumers for its flesh quality and low-fat content. It presents a high-value market (Cerqueira, Tsuzuki, 2009). In addition, since fat snook is euryhaline, it can be inhabited inland, away from the coast (Wosnick et al., 2018), and using anesthetics can facilitate management and transportation procedures in this species by reducing stress.

This study aimed to evaluate the efficacy of EOLA as an anesthetic for fat snook juveniles, analyzing time to induce anesthesia, ventilatory rate (VR), and biochemical, antioxidant, and oxidative stress parameters. To our knowledge, this is the first study describing EOLA’s anesthetic activity in a euryhaline fish.

MATERIAL AND METHODS

Locations, and animals. Vouchers were deposited in the ichthyological collection of the Museu de Biologia Professor Mello Leitão of the Universidade Vila Velha UVV), Vila Velha, ES, Brazil (MBML 12877). Two hundred and thirty-two fat snook specimens were purchased from the Laboratório de Piscicultura Marinha (LAPMAR), Florianópolis, SC, Brazil, and transferred to the Laboratório de Ictiologia Apliacada at UVV. During acclimation (ten days), fish were distributed in six 500 L fiberglass tanks containing 400 L of water, with constant aeration and physical and biological filters. The animals were fed commercial feed containing 54% crude protein (INVE Aquaculture Nutrition, Salt Lake City, USA) three times a day (08:00, 12:00, and 17:00 h) until apparent satiety.

The water quality parameters were measured during the acclimatization (twice a week) and experimental (every day) periods and remained stable. The water quality parameters for dissolved oxygen (5.92 ± 0.13 mg O2 L−1; 73.98 ± 1.41% saturation) and water temperature (26.83 ± 0.05 °C) were monitored with the aid of an oximeter (YSI oximeter OD 200), pH (7.33 ± 0.04) with the assistance of a pH meter (YSI pH 100), and conductivity (33.88 ± 1.17 µS cm−1) and salinity (30.28 ± 0.05 ppt) using a conduct meter (YSI conductivity EC 300). Total ammonia (0.25 ± 0.05 mg N-NH3 L−1) was measured by the indophenol method, and alkalinity (117.34 ± 3.10 mg CaCO3 L−1), and nitrite (0.22 ± 0.08 mg N−NO2 L−1) by titration using colorimetric reactions from a commercial kit (Alcon Ltd. – Camboriú, SC, Brazil). The tanks were cleaned daily at 17:30 h with a siphon to remove excess feces and residues.

Essential oil from Lippia alba.The specimens of L. alba were cultivated in Frederico Westphalen, RS, Brazil, and its leaves were collected in January 2013 (summer). The EOLA was obtained from fresh plant leaves by hydrodistillation for 2 h using a Clevenger-type apparatus (European Pharmacopeia, 2007). The EOLA was stored at -20 °C until composition analysis and biological assays. The chemical composition of EOLA was determined using gas chromatography-mass spectrometry (GC-MS), as described in detail by Simões et al. (2017). The same EOLA investigated in the current study was used in a previous study (Simões et al., 2017); its main constituents are linalool (48.69%), eucalyptol (10.51%), β-myrcene (9.70%), and β-caryophyllene (4.19%).

Experimental procedures. Before use, EOLA was diluted 1:10 in absolute ethanol. First, pilot tests (n = 6 fish per concentration) were performed in aquariums containing 1 L of water and constant aeration under conditions similar to those of experiments to choose the most appropriate concentrations to be used in the experiments. The pilot test tested the following concentrations: 10, 20, 30, 40, 50, 60, 80, 100, 120, 130, 140, 150, 160, 180, 200, 220, 230, and 250 µL EOLA L-1. A control group was submitted to the same handling process using water only. The concentrations above 30 µL EOLA L-1 caused anesthesia, while 10 and 20 µL EOLA L-1 caused only sedation. In this study, four experiments were performed. Only small fish (6.03 ± 0.09 g; 9.30 ± 0.05 cm) were used in experiments 1, 3, and 4. In experiment 2, larger fish (38.49 ± 2.07 g; 16.55 ± 0.26 cm) were also used. The same observers accompanied the experiments. Before each experiment, the fish fasted for 24 h.

Experiment 1: Anesthetic induction. Seventy animals were used to test six different EOLA concentrations: 30, 80, 130, 180, 200, and 230 μL L−1. A control group was transferred to aquariums containing only ethanol (2,070 μL L−1) at a concentration equivalent to the dilution used for 230 μL L−1, totaling 7 treatments. The procedure involved transferring fish (n = 10 per treatment, with one fish used at a time) to aquariums containing 1 L of water and constant aeration. Mild anesthesia (partial loss of balance and erratic swimming) and deep anesthesia (complete loss of balance and cessation of swimming) were evaluated according to Small (2003).

The animals that reached deep anesthesia were rinsed in clean water and transferred to recovery aquariums containing 3 L of water without EOLA and constant aeration to estimate the recovery time (behavior similar to the fish kept in the maintenance tanks, i.e., swimming and equilibrium without alterations). The lowest concentration, capable of inducing partial loss of balance and erratic swimming without causing deep anesthesia, was indicated for mild anesthesia in fish. For deep anesthesia in fish, we chose the lowest concentration capable of causing complete loss of balance and cessation of swimming in less than 3 min and with a recovery of less than 5 min (Small, 2003; Sena et al., 2016).

Experiment 2: Test with larger fish. For this experiment, larger fish (n = 10) were exposed to the concentration indicated in experiment 1 for smaller fish, against which they were compared (i.e., 180 μL EOLA L−1). The evaluations for anesthesia and recovery were performed following the same procedures described in experiment 1, where the anesthesia and recovery times were compared between larger and smaller fish. However, aquariums containing 3 L of water were used to evaluate anesthesia, and aquariums with 6 L of water were used to assess recovery. Both aquariums had constant aeration.

Experiment 3: Ventilatory rate (VR). Tests were conducted in aquariums with 5 L of water and constant aeration. In each aquarium, squares (4.8 cm2) were marked in the background and behind to determine the VR of the fat snook exposed to 5 and 10 μL EOLA L−1. In a pilot study, these concentrations caused only sedation (decreased reactivity to external stimuli) (Small, 2003). Two control groups were also evaluated; one control group was kept only in water, and another was kept in water plus ethanol (90 μL L−1). The VR (count of opercular movements in beats min−1) was individually analyzed in 32 fat snooks (n = 8 per treatment) at 0, 20, 40, 60, and 120 min during exposure to the anesthetic solution. At one time, fish was filmed concurrently from the front and above the same test aquarium for 10 min each time.

Experiment 4: Stress responses. One hundred and twenty animals were exposed individually to 30 and 180 μL EOLA L−1 (concentrations indicated for mild and deep anesthesia, respectively; see results of the first experiment) in aquariums containing 1 L of water and constant aeration. A control group was transferred to aquariums (1 L) containing only ethanol (1,620 μL L−1). A second control group with fish kept in aquariums (1 L) containing only water was also performed. After the fish reached deep anesthesia or after 4 min (control groups), the animals were transferred to recovery aquariums (3 L) without EOLA. The fish were evaluated at 0, 30, and 60 min after anesthesia recovery for blood glucose, whole-body cortisol, antioxidant enzymes, and oxidative stress parameters.

Experimental analysis in experiment 4. As the fish were small, collecting only one drop of blood (from the caudal vein) was possible. Blood was collected in fish after transferring to recovery aquariums at 0 (no recovery), 30, and 60 min. Blood glucose levels were analyzed of 5 fish per treatment at each time using microfilm strips and a digital glucometer (Accu-Chek Active, Roche ™) immediately after blood collection and are expressed as mg dL−1. Then, fish were euthanized with lethal benzocaine hydrochloride (250 mg L−1) and frozen at -80 ºC for future analysis of whole-body cortisol.

The whole-body cortisol was extracted using the validated method described by Sink et al. (2007). This method was chosen due to inadequate blood volume to measure plasma cortisol accurately. This method was selected because the fish were too small to collect enough blood for plasma analysis. The mean detection accuracy of spiked samples was 94.3%, which was tested by calculating the recoveries from samples spiked with known amounts of cortisol (50, 25, and 12.5 ng mL−1). All values were adjusted for recovery following the “Cortisol value = measured value × 1.0604” equation. Whole-body cortisol levels were measured (in duplicate) using a commercially available enzyme-linked immunosorbent assay kit (EIAgen™ Cortisol test, BioChem Immuno Systems). The whole-body cortisol levels are expressed as ng g of tissue−1. This kit was fully validated for fish tissue extracts using the method proposed by Sink et al. (2007) and described in detail by Parodi et al. (2014). There was a strong positive correlation between the curves (R2 = 0.89), and the samples had low inter- (CV of 7–10%) and intra-assay (CV of 5–9%) variations.

Another 5 fish per treatment each time were euthanized with the lethal concentration of benzocaine hydrochloride for liver collection. The liver was collected at the exact times of the blood collection and then preserved at -80 ºC until analysis of oxidative stress parameters.

The liver was weighed and homogenized (1:4, w/v) in Tris buffer 20 mM (pH 7.4), sucrose 0.5 mM, KCl 0.15 mM, and 1 mM protease inhibitor (PMSF). The samples were centrifuged at 10,000 x g for 20 min (4 °C). The resulting supernatant fraction was used for glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) assays. All assays (in triplicate) were carried out using a spectrophotometer (Spectramax Plus 384, Molecular Devices) at 25 °C.

Protein content was determined by the Bradford method (Bradford, 1976) adapted to the microplate. Enzymatic activities were determined at 25 °C and expressed as activity per mg of protein. The samples showed no differences in protein content.

The LPO was assessed by Fe2+ oxidation in the presence of xylenol orange (FOX, ferrous oxidation-xylenol orange assay) as described by Jiang et al. (1991). The homogenized samples were treated with 10% trichloroacetic acid and centrifuged. The supernatants were applied to a solution containing 900 mL of FOX reagent in 90% (v/v) methanol and incubated at 37 °C for color development prior to colorimetric measurement at 560 nm. The LPO concentrations were expressed as nmol mg protein-1.

The GST activity was determined by measuring the increase in absorbance at 340 nm, incubating reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates (Keen et al., 1976). The enzyme activity was calculated as µmol GS-DNB min−1 mg protein−1 using a molar extinction coefficient of 9.6 mM−1 cm−1.

The CAT activity was determined following the method described by Beutler (1975), based on the consumption of H2O2 recorded at 240 nm. The CAT activity was defined as the difference in the absorbance per unit of time (extinction coefficient 40 mM−1 cm−1) and expressed as μmol min−1 mg protein−1.

The SOD activity was determined, according to McCord, Fridovich (1969), by measuring the absorption of the reduction of cytochrome C by the xanthine oxidase/hypoxanthine system at 550 nm. One unit of SOD is the amount of the enzyme that inhibits by 50% the reduction of cytochrome C. The SOD activity was expressed as IU mg protein−1.

Statistical analyses. All data are presented as mean ± standard error of the mean (SEM). Levene’s test tested the homogeneity of variances between treatments. Experiments 1 and 2 were analyzed using one-way ANOVA, while experiments 3 and 4 were analyzed using a two-way ANOVA (time × treatment). After ANOVA, Tukey post hoc tests were performed. In addition, experiment 1 (mild and deep anesthesia) was also evaluated by power regression analysis (concentration × time). Significance was set at a critical level of 95% (P < 0.05).

RESULTS

Fish showed no mortality during or after 72 h of exposure to EOLA in the experiments.

Experiment 1: Anesthetic induction. Applying 2,070 μL L−1 of ethanol alone did not induce sedation or anesthesia. The regression results showed that higher concentrations of EOLA resulted in a shorter time for fish anesthesia. However, no significant relationship was found between the EOLA concentrations and the anesthetic recovery time. At a 30 μL EOLA L−1 concentration, the fish reached mild anesthesia at 127.4 s. Fat snooks were deeply anesthetized only at concentrations above 80 μL EOLA L−1. The concentrations of 180 μL EOLA L−1 induced the shortest deep anesthesia and recovery times, with times of 184.6 and 163.4 s, respectively (P < 0.05) (Fig. 1).

Experiment 2: Tests with larger fish. Fat snooks of larger size showed mild and deep anesthesia and recovery times significantly higher than those of smaller size when exposed to 180 μL EOLA L−1 (P < 0.05) (Fig. 2).

Experiment 3: Ventilatory rate (VR). Comparing the differences between treatments, the VR was significantly higher in fish at 10 μL EOLA L−1 at times between 20 and 120 min of recovery and at 5 μL EOLA L−1 at times of 60 and 120 min of recovery compared to the control and ethanol groups (P < 0.05).

The two tested EOLA concentrations also showed differences over time. The VR was significantly higher in fish exposed to 5 μL EOLA L−1 at times between 40 and 120 min, compared to 0 min (P < 0.05). Similarly, the VR in fish at 10 μL EOLA L−1 was significantly lower at 0 min than at other times (P < 0.05). In addition, at the time 20 min to 5 μL EOLA L−1 and at times 20 and 40 min to 10 μL EOLA L−1, the VR was significantly lower than at the time 120 min of recovery (P < 0.05) (Fig. 3).

Experiment 4: Blood glucose and whole-body cortisol. Blood glucose levels were significantly higher in fish anesthetized with 180 than with 30 μL EOLA L−1 at all the times evaluated (P < 0.05). The treatment 180 μL EOLA L−1 also showed blood glucose levels significantly higher than the ethanol group (1,620 μL L−1) at 0 min and the control group at 60 min after recovery (P < 0.05). Fish exposed to 30 μL EOLA L−1 had blood glucose levels significantly lower than other groups at times 30 and 60 min after recovery (except for the ethanol group at the time 30 min) (P < 0.05). At the time 0 min, the values of blood glucose were significantly lower in all groups than at the time 30 min, and this difference was maintained at the time 60 min for fish exposed to ethanol and 180 μL EOLA L−1 (P < 0.05) (Fig. 4A).

FIGURE 1 |
Time (s) required for mild and deep anesthesia and recovery in fat snook angelfish (Centropomus parallelus) with increasingly essential oil from Lippia alba (EOLA) concentrations. Data are presented as the mean ± SEM (n = 10 fish per treatment). Different letters indicate significant differences between treatments. One-way ANOVA and Tukey’s tests were used to determine statistical significance (P < 0.05). Mild and deep anesthesia times showed regression.

FIGURE 2 |
Time (s) required for mild and deep anesthesia and recovery in fat snook (Centropomus parallelus) exposed to essential oil from Lippia alba (180 µL L−1). Smaller fish = 6.03 ± 0.09 g; 9.30 ± 0.05 cm. Larger fish = 38.49 ± 2.07 g; 16.55 ± 0.26 cm. Data are presented as the mean ± SEM (n = 10 fish per treatment). Different letters indicate significant differences between fish body size classes. One-way ANOVA and Tukey’s tests were used to determine statistical significance (P < 0.05).

FIGURE 3 |
Ventilatory rate (VR) of fat snook (Centropomus parallelus) during exposure to the essential oil from Lippia alba (EOLA). Data are presented as the mean ± SEM (n = 8 fish per treatment). Capital letters indicate significant differences between time points within the same treatment. Lowercase letters indicate significant differences between treatments at the same time point. Two-way ANOVA and Tukey’s tests were used to determine statistical significance (P < 0.05).

The whole-body cortisol values at 0 min were significantly lower in the ethanol group than in fish exposed to 30 μL EOLA L−1 (P < 0.05). Fish exposed to 180 μL EOLA L−1 had whole-body cortisol levels significantly higher than those exposed to 30 μL EOLA L−1 at 30 min and the control group at 60 min after recovery (P < 0.05). In the control and ethanol groups, whole-body cortisol levels were significantly higher at 30 min than at 0 min (P < 0.05). In the fish anesthetized with 180 μL EOLA L−1, whole-body cortisol levels were significantly higher at times 30 and 60 min than at the time 0 min (P < 0.05) (Fig. 4B).

FIGURE 4 |
Blood glucose (A) and whole-body cortisol (B) levels after transferring to recovery aquariums of anesthetized fat snook (Centropomusparallelus) with essential oil from Lippiaalba (EOLA). Data are presented as the mean ± SEM (n = 5 fish per treatment each time). Capital letters indicate significant differences between time points within the same treatment. Lowercase letters indicate significant differences between treatments at the same time point. Two-way ANOVA and Tukey’s tests were used to determine statistical significance (P < 0.05).

Experiment 4: Oxidative stress parameters. At 30 min after recovery, the fat snooks exposed to 30 μL EOLA L−1 had significantly higher liver GST activity than the fish submitted to the other treatments at the same time (30 min) or than this same treatment (30 μL EOLA L−1) at 0 min (P < 0.05). At 60 min, the group 180 μL EOLA L−1 had significantly higher liver GST activity than the control and ethanol groups at the same time (60 min) or than this same treatment (180 μL EOLA L−1) at other times (P < 0.05) (Fig. 5A). At 60 min of recovery, the fish from the EOLA groups had significantly higher liver SOD activity than the control group (only water). Still, they did not differ from the ethanol group (P < 0.05). In addition, fish exposed to 30 μL EOLA L−1 showed significantly higher liver SOD activity at 60 min than at 0 min (P < 0.05) (Fig. 5B). The different treatments did not change liver CAT and LPO levels (P > 0.05) (Figs. 5C, D).

FIGURE 5 |
Antioxidant and oxidative stress parameters in the liver after transferring to recovery aquariums of fat snook (Centropomusparallelus) anesthetized with the essential oil from Lippiaalba (EOLA). A = GST (glutathione S-transferase). B = SOD (superoxide dismutase). C = CAT (catalase). D = LPO (lipid peroxidation). Data are presented as the mean ± SEM (n = 5 fish per treatment each time). Capital letters indicate significant differences between time points within the same treatment. Lowercase letters indicate significant differences between treatments at the same time point. Two-way ANOVA and Tukey’s tests were used to determine statistical significance (P < 0.05).

DISCUSSION

Various studies have indicated that an anesthetic is more effective when it has fast action (< 3 min) and a short recovery time (< 5 or 10 min) (Small, 2003; Ross, Ross, 2009; Sena et al., 2016; Teixeira et al., 2017; Oliveira et al., 2019b). In addition, lower anesthetic concentrations may provide a higher safety margin for fish welfare and avoid essential oil wastage (Teixeira et al., 2017). In this sense, the present study recommends 180 μL EOLA L−1 as the minimum effective concentration for deep anesthesia of fat snook. In addition, considering that the concentration used for long-term anesthesia should be the minimum possible to avoid deep anesthesia (Oliveira et al., 2019a), 30 μL EOLA L−1 is viable for mild anesthesia in this species. In line with our results, previous studies also found EOLA as a potential anesthetic for silver catfish (300 μL EOLA L−1 (Cunha et al., 2010; Heldwein et al., 2014; Souza et al., 2018), tambacu (200 μL EOLA L−1) (Sena et al., 2016), seahorse (150 μL EOLA L−1) (Cunha et al., 2011), gilthead sea bream (100-200 μL EOLA L−1) (Toni et al., 2015), and Nile tilapia (500 μL EOLA L−1) (Hohlenwerger et al., 2016).

In the current study, the main compound of EOLA was linalool (48.69%). Linalool is a constituent of several essential oils whose depressor activities on the CNS are well-described in rodents and humans (Dobetsberger, Buchbauer, 2011). Souza et al. (2018) verified that EOLA chemotype linalool is a safe and effective anesthetic since it did not significantly change the expression of several hypothalamus-pituitary-interrenal (HPI) axis genes in silver catfish. The anesthetic effect of EOLA is related to the GABAergic system in silver catfish (Heldwein et al., 2012). Still, Heldwein et al. (2014) did not detect the direct interaction of linalool with the benzodiazepine site of GABA receptors in the same species. Eucalyptol (the second main compound in EOLA in this study; 10.51%) has anticonvulsive effects in mice (Galindo et al., 2010). Myrcene (the third main compound in EOLA in this study; 9.70%) acts at both central and peripheral sites, mediating endogenous opioids and α2-adrenoreceptors in mice (Rao et al., 1990). So, linalool could have interacted with other compounds (such as eucalyptol and β-myrcene) to cause the anesthetic effects in fat snook.

Several factors can affect the time for fish to reach anesthesia, such as water quality (Sneddon, 2012), essential oil composition (Limma Netto et al., 2016; Souza et al., 2018), and size (Sneddon, 2012; Oliveira et al., 2022). Body weight is a determining factor in defining the best time for anesthetic induction, where smaller fish would be more easily anesthetized (Tarkhani et al., 2016). This information agrees with the current study because larger fish (with higher body weight) took more time to be anesthetized than smaller fish. Therefore, 180 μL EOLA L−1 is the ideal concentration to anesthetize larger fish. The gill surface of smaller fish is proportionally more prominent to the body than in larger fish (Hoseini et al., 2013), maximizing the contact and diffusion capacity of the essential oil. This action was corroborated by our results, where larger fat snooks took about twice as long as smaller ones to be mildly and deeply anesthetized and recover from anesthesia. Similarly, larger freshwater angelfish Pterophyllum scalare (Schultze, 1823) showed longer anesthesia and recovery times than smaller ones (Oliveira et al., 2022).

Anesthetics (regardless of causing mild or deep anesthesia) at low concentrations commonly have a sedative effect (Oliveira et al., 2019b). Concentrations between 10 and 20 μL EOLA L−1 were indicated for transporting silver catfish, seahorse, Nile tilapia, and tambacu (Cunha et al., 2010, 2011; Becker et al., 2012; Hohlenwerger et al., 2016; Sena et al., 2016). These same concentrations caused sedative effects for fat snook in a pilot test, and, therefore, we investigated their impact on the VR. In fish, sedative substances can reduce VR and metabolic stress (Becker et al., 2018). Essential oils at low concentrations (causing only sedation) reduced VR: EOLA (10–20 μL EOLA L−1) in Nile tilapia (Hohlenwerger et al., 2017) and silver catfish (5–10 μL EOLA L−1) (Becker et al., 2018), essential oil from Aloysia triphylla (Aloysia citrodora Paláu) in Nile tilapia (20–30 μL EOLA L−1) (Teixeira et al., 2018), and essential oil from Lippia sidoides (syn. Lippia origanoides Kunth) in angelfish freshwater (10 and 15 mg EOLA L−1) (Oliveira et al., 2022). On the other hand, in the current study, 10–20 μL EOLA L−1 increased VR.

A possible explanation for this finding would be that the presence of the anesthetic in the water could cause transitory stress, increasing the VR (Sneddon, 2012; Becker et al., 2018). A consequence of increased VR is increased oxygen absorption from the water. Higher oxygenation should increase the tissue oxygen concentration, a precursor to ROS (Nitz et al., 2020a). In this situation, animals could increase their antioxidant defenses to avoid damage to cellular homeostasis. This was verified in the present study when the fish were anesthetized with 30 and 180 μL EOLA L−1, where exposure time and anesthetic concentration strongly influenced liver GST and SOD activity. On the other hand, the anesthetic did not change liver CAT and LPO values. GST is an important enzyme catalyzing LPO products and other metabolites and transforming xenobiotics into more easily excreted substances (Lushchak et al., 2009). SOD and CAT protect against oxidative damage (Pandey et al., 2003). The LPO acts as a cell lesion mechanism provoked by free oxygen radicals (Copatti et al., 2019). If the antioxidant system does not work well, LPO, which is highly toxic for fish, may occur (Mirzargar et al., 2022). In the current study, the cellular function must not have been impaired in fat snook exposed to EOLA, as liver LPO levels did not differ from non-anesthetized groups. The results also suggest that EOLA did not cause oxidative stress in this species. Although EOLA did not influence liver CAT activity, the increase of liver GST linked to SOD activity could minimize oxidative damage (LPO) during temporary changes resulting from physiological and biochemical adjustments of recovery from anesthesia (Souza et al., 2018), contributing to the primary antioxidant defense system.

Although few studies evaluated antioxidant responses and oxidative stress in anesthetized fish with linalool chemotype EOLA, the results found by these authors were similar to those recorded in our study. Anesthesia with EOLA showed an increase in the antioxidant capacity of silver catfish, increasing liver GST, SOD, and CAT activity, besides reducing liver LPO levels (Azambuja et al., 2011; Salbego et al., 2017; Souza et al., 2018) and cururu stingray Potamotrygon wallacei Carvalho, Rosa & Araújo, 2016, increasing brain SOD, and CAT activity, besides reducing brain LPO levels (Finamor et al., 2023). Therefore, in an integrative analysis of our study with the studies mentioned, it is demonstrated that EOLA can potentially improve antioxidant responses in fish anesthesia.

In addition, Oliveira et al. (2022) verified that the essential oil from L. sidoides (a plant of the same genus used in this study) can cause irreversible changes in gills. A commitment of branchial O2-sensitive chemoreceptors can lead to a greater VR in fish because these structures exert dominant control over ventilatory reflexes (Burleson, Smatresk, 2000). However, there is still no proof that the branchial changes provoked by anesthetics cause detrimental effects on the O2-sensitive chemoreceptors. Another possibility for the increase of VR verified in the current study would be the increases in arterial and venous O2 tension of fat snook, which can cause hyperventilation (Burleson, Smatresk, 2000). A situation that causes hyperoxygenation commonly increases the fish metabolism (Nitz et al., 2020b) because, during recovery, fish can increase VR immediately to blow off CO2 to maintain acid-base balance (Burleson, Smatresk, 2000). The fish of the present study were kept in aquariums with continuous aeration during the sedation recovery period. The aeration could have contributed to increasing gill oxygenation, with a consequent increase in VR to reinforce metabolic demand.

Similarly, Kiessling et al. (2009) verified higher VR in Atlantic salmon Salmo salar Linnaeus, 1758 during recovery from anesthesia with benzocaine, MS-222, and isoeugenol. Becker et al. (2012) showed an increase in VR in silver catfish after 30 min of sedation with EOLA or eugenol; however, the VR was reduced at 60 min of exposure. Thus, our data indicate that fish can utilize VR to compensate for gill alterations or acid-base disturbances, such as during anesthesia recovery. In addition, we evaluated only smaller fish, which should have a higher VR than larger fish.

It is recognized that using anesthetics in fish is much more complex than previously described in the literature (Readman et al., 2017). In fish, using anesthetics is usually pointed as a stress reducer (Sena et al., 2016; Hohlenwerger et al., 2017). However, their direct application can increase stress responses since unventilated anesthesia causes depression of the CNS, impairing net ion fluxes, VR, and metabolism (Ross, Ross, 2009; Teixeira et al., 2018; Oliveira et al., 2022). Cortisol is the leading indicator of primary stress responses in fish and involves a series of neuroendocrine responses. It can trigger glycogenolysis and gluconeogenesis and increase blood glucose levels (Sena et al., 2016; Teixeira et al., 2017; Oliveira et al., 2019a). Additionally, this hormone is crucial for euryhaline fish since it interacts with other hormones (e.g., growth hormone, prolactin) and stimulates an increase in the functional area of ionocytes and a decrease in the gill permeability to maintain ionic balance (McCormick et al., 2008; Copatti, Baldisserotto, 2021).

Euryhaline fish can maintain blood glucose levels constant within their optimum salinity range (Herrera et al., 2009). However, if the anesthetic does not suppress the activation of the HPI axis during stress, a rapid release of catecholamines and cortisol might occur, increasing glucose metabolism (Barton, 2002). In our study, 180 μL EOLA L−1 could not avoid stress because it increased whole-body cortisol and blood glucose levels during recovery. Whole-body cortisol techniques have been previously used to evaluate the stress of small fishes, being useful as a general indicator of stress (Ramsay et al., 2006; Baldisserotto et al., 2014). A stress event, such as handling or fish perception of anesthetic presence (Souza et al., 2018), can stimulate catecholamine and cortisol release, which induces liver glycogenolysis and an increase of blood glucose (Wendeelar Bonga, 1997; Barton, 2002) to ensure the energy supply with a possible higher metabolic demand. Another possibility for the rise in whole-body cortisol is its relationship with VR, which, when increased, can cause a more rapid recovery from anesthesia and thereby trigger an ability to react to external signals and pay the oxygen debt acquired during the anesthesia (Kiessling et al., 2009). In the present study, the anesthesia caused stress in fat snooks, increasing oxygen demand, as demonstrated by our results for VR and blood glucose levels.

Previous studies performed with fat snook found similar results. Wosnick et al. (2018) reported a relationship between the increase in blood cortisol and glucose levels after anesthesia with benzocaine (50 mg L-1), regardless of the salinity of exposure of the fish (5-30 ppt). Elevated blood glucose levels were also observed in transporting fish with essential oil from Nectandra megapotamica (Spreng.) Mez (300 μL L−1), whose values were higher in individuals adapted to seawater than freshwater (Tondolo et al., 2013). Parodi et al. (2016) verified that adding essential oil from A. triphylla (20 μL L-1), although it had reduced blood cortisol levels, increased blood glucose levels in fat snook after transport. Interestingly, both EOLA and the essential oil from A. triphylla are not aversive to silver catfish and zebrafish, Danio rerio (Hamilton, 1822) (Bandeira Junior et al., 2018) and avoided plasma cortisol rise in silver catfish (Cunha et al., 2010; Parodi et al., 2014, respectively), but EOLA was not as efficient for reduce cortisol levels in fat snooks (current study), tambacu (Sena et al., 2016), and meagre (EOLA) (Cárdenas et al., 2016), demonstrating that the effect of anesthetics can be different according to the management conditions (e.g., anesthetic chemical compounds, anesthetic concentration, salinity, ambient temperature) and species.

In conclusion, the best mild and deep anesthesia times in fat snook juveniles for EOLA were obtained with 30 and 180 μL EOLA L−1, respectively, and these times were lower in smaller than larger fish. The EOLA (mainly at the highest concentration) increased VR, whole-body cortisol, blood glucose, and liver GST and SOD values. The transfer of EOLA across the gills is presumably regulated by branchial ventilation; therefore, the increase of VR is possibly related to the rise of whole-body cortisol and blood glucose levels in fish anesthetized with 180 μL EOLA L−1. Finally, the rise of liver GST and SOD activities found in fish exposed to 30 and 180 μL EOLA L−1 demonstrated that EOLA effectively prevented oxidative stress and can be used for anesthesia in fat snook.

ACKNOWLEDGEMENTS

This research was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (finance code 001, CAPES), which provided a doctoral fellowship to L.N. Simões-Bueno and R.D. Amanajás; and Conselho Nacional de Desenvolvimento Tecnológico (CNPq), which awarded research fellowships to CEC, BHH, BOC, ALV, and BB. Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS/CAPES, process 19/2551-0000655-1); INCT-ADAPTA (CNPq process 465540/2014-7), and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM process 062.01187/2017).

REFERENCES

• Azambuja CR, Mattiazzi J, Riffel APK, Finamor IA, Garcia LO, Heldwein CG, Heinzmann BM, Baldisserotto B, Pavanato MA, Llesuy SF. Effect of the essential oil of Lippia alba on oxidative stress parameters in silver catfish (Rhamdia quelen) subjected to transport. Aquaculture. 2011; 319:156–61. https://doi.org/10.1016/j.aquaculture.2011.06.002
  » https://doi.org/10.1016/j.aquaculture.2011.06.002
• Baldisserotto B, Brinn RP, Brandão FR, Gomes LC, Abreu JS, McComb DM, Marcon JL. Ion flux and cortisol responses of cardinal tetra, Paracheirodon axelrodi (Schultz) to additives (tetracycline, tetracycline + salt or Amquel®) used during transportation: contributions to Amazonian ornamental fish trade. J Appl Ichthyol. 2014; 30(1):86–92. https://doi.org/10.1111/jai.12282
  » https://doi.org/10.1111/jai.12282
• Bandeira Junior G, de Abreu MS, da Rosa JGS, Pinheiro CG, Heinzmann BM, Caron BO, Baldisserotto B, Barcellos LJG. Lippia alba and Aloysia triphylla essential oils are anxiolytic without inducing aversiveness in fish. Aquaculture. 2018; 482:49–56. https://doi.org/10.1016/j.aquaculture.2017.09.023
  » https://doi.org/10.1016/j.aquaculture.2017.09.023
• Barton BA. Stress in fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integr Comp Biol. 2002; 42(3):517–25. https://doi.org/10.1093/icb/42.3.517
  » https://doi.org/10.1093/icb/42.3.517
• Batista ES, Brandão FR, Majolo C, Inoue LAKA, Maciel PO, Oliveira MR, Chaves FCM, Chagas EC. Lippia alba essential oil as anesthetic for tambaqui. Aquaculture. 2018; 495:545–49. https://doi.org/10.1016/j.aquaculture.2018.06.040
  » https://doi.org/10.1016/j.aquaculture.2018.06.040
• Becker AJ, Fogliarini CO, Souza CF, Becker AG, Mourão RHV, Silva LVF, Baldisserotto B. Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: A comparative approach between different essential oils. Rev Bras Zootec. 2018; 47:e20170185. https://doi.org/10.1590/rbz4720170185
  » https://doi.org/10.1590/rbz4720170185
• Becker AG, Parodi TV, Heldwein CG, Zeppenfeld CC, Heinzmann BM, Baldisserotto B. Transportation of silver catfish, Rhamdia quelen, in water with eugenol and the essential oil of Lippia alba Fish Physiol Biochem. 2012; 38:789–96. https://doi.org/10.1007/s10695-011-9562-4
  » https://doi.org/10.1007/s10695-011-9562-4
• Beutler E. Red cell metabolism. A manual of biochemical methods. Grune & Stratton Inc., New York; 1975.
• Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72(1–2):248–54. https://doi.org/10.1006/abio.1976.9999
  » https://doi.org/10.1006/abio.1976.9999
• Burleson ML, Smatresk NL. Branchial chemoreceptors mediate ventilatory responses to hypercapnic acidosis in channel catfish. Comp Biochem Physiol. A. 2000; 125(3):403–14. https://doi.org/10.1016/S1095-6433(00)00167-7
  » https://doi.org/10.1016/S1095-6433(00)00167-7
• Cárdenas C, Toni C, Martos-Sitcha JA, Cárdenas S, de las Heras V, Baldisserotto B, Heinzmann BM, Vázquez R, Mancera JM. Effects of clove oil, essential oil of Lippia alba and 2-phenoxyethanol anesthesia on juvenile meagre, Argyrosomus regius (Asso, 1801). J Appl Ichthyol. 2016; 32(4):693–700. https://doi.org/10.1111/jai.13048
  » https://doi.org/10.1111/jai.13048
• Cerqueira VR, Tsuzuki MY. A review of spawning induction, larviculture, and juvenile rearing of the fat snook, Centropomus parallelus Fish Physiol Biochem. 2009; 35:17–28. https://doi.org/10.1007/s10695-008-9245-y
  » https://doi.org/10.1007/s10695-008-9245-y
• Chowdhury S, Saikia SK. Oxidative stress in fish: A review. J Scient Res. 2020; 12(1):145–60. https://doi.org/10.3329/jsr.v12i1.41716
  » https://doi.org/10.3329/jsr.v12i1.41716
• Copatti CE, Baldisserotto B. Osmoregulation in tilapia: Environmental factors and internal mechanisms. In: López-Olmeda JF, Sánchez-Vázquez FJ, Fortes-Silva R, editors. Biology and aquaculture of tilapia. CRC Press, Boca Raton; 2021. p.104–118.
• Copatti CE, Baldisserotto B, Souza CF, Monserrat JM, Garcia L. Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu (Piaractus mesopotamicus). Neotrop Ichthyol. 2019; 17(4):190032. https://doi.org/10.1590/1982-0224-20190032
  » https://doi.org/10.1590/1982-0224-20190032
• Cunha MA, Barros FM, Garcia LO, Veeck APL, Heinzmann BM, Loro VL, Emanuelli T, Baldisserotto B. Essential oil of Lippia alba: A new anesthetic for silver catfish, Rhamdia quelen Aquaculture. 2010; 306:403–06. https://doi.org/10.1016/j.aquaculture.2010.06.014
  » https://doi.org/10.1016/j.aquaculture.2010.06.014
• Cunha MA, Silva BF, Delunardo FAC, Benovit SC, Gomes LC, Heinzmann BM, Baldisserotto B. Anesthetic induction and recovery of Hippocampus reidi exposed to the essential oil of Lippia alba Neotrop Ichthyol. 2011; 9(3):683–88. https://doi.org/10.1590/S1679-62252011000300022
  » https://doi.org/10.1590/S1679-62252011000300022
• Dobetsberger C, Buchbauer G. Actions of essential oils on the central nervous system: An updated review. Flavour Fragr J. 2011; 26(5):300–16. https://doi.org/10.1002/ffj.2045
  » https://doi.org/10.1002/ffj.2045
• European Pharmacopoeia. 6th ed. European directorate for the quality of medicines, Strasbourg; 2007.
• Finamor IA, Bressan CA, Ariotti K, Lima CL, Schmidt D, Heinzmann BM, Baldisserotto B, Marcon JL, Pavanato MA. The long-term transport of Potamotrygon wallacei increases lactate levels and triggers oxidative stress in its brain: The protective role of recovery and the essential oil of Lippia alba Aquaculture. 2023; 572:739461. https://doi.org/10.1016/j.aquaculture.2023.739461
  » https://doi.org/10.1016/j.aquaculture.2023.739461
• Galindo LA, Pultrini AM, Costa M. Biological effects of Ocimum gratissimum L. are due to synergic action among multiple compounds present in essential oil. J Nat Med. 2010; 64:436–41. https://doi.org/10.1007/s11418-010-0429-2
  » https://doi.org/10.1007/s11418-010-0429-2
• Heldwein CG, Silva LL, Gai EZ, Roman C, Parodi TV, Burger ME, Baldisserotto B, Flores EM, Heinzmann BM. S-(+)-Linalool from Lippia alba: sedative and anesthetic for silver catfish (Rhamdia quelen). Vet Anaesth Analg. 2014; 41(6):621–29. https://doi.org/10.1111/vaa.12146
  » https://doi.org/10.1111/vaa.12146
• Heldwein CG, Silva LL, Reckziegel P, Barros FM, Bürger ME, Baldisserotto B, Mallmann CA, Schmidt D, Caron BO, Heinzmann BM. Participation of the GABAergic system in the anesthetic effect of Lippia alba (Mill.) N.E. Brown essential oil. Braz. J Med Biol Res. 2012; 45(5):436–43. https://doi.org/10.1590/s0100-879x2012007500052
  » https://doi.org/10.1590/s0100-879x2012007500052
• Herrera M, Vargas-Chacoff L, Hachero I, Ruiz-Jarabo I, Rodiles A, Navas JI, Mancera JM. Osmoregulatory changes in wedge sole (Dicologoglossa cuneata Moreau, 1881) after acclimation to different environmental salinities. Aquac Res. 2009; 40(7):762–71. https://doi.org/10.1111/j.1365-2109.2008.02147.x
  » https://doi.org/10.1111/j.1365-2109.2008.02147.x
• Hohlenwerger JC, Baldisserotto B, Couto RD, Heinzmann BM, Silva DT, Caron BO, Schimidt, D, Copatti CE. Essential oil of Lippia alba in the transport of Nile tilapia. Ciênc Rural. 2017; 47:20160040. https://doi.org/10.1590/0103-8478cr20160040
  » https://doi.org/10.1590/0103-8478cr20160040
• Hohlenwerger JC, Copatti CE, Sena AC, Couto RD, Baldisserotto B, Heinzmann BM, Caron BO, Schmidt D. Could the essential oil of Lippia alba provide a readily available and cost-effective anaesthetic for Nile tilapia (Oreochromis niloticus)? Mar Freshw Behav Physiol. 2016; 49(2):119–26. https://doi.org/10.1080/10236244.2015.1123869
  » https://doi.org/10.1080/10236244.2015.1123869
• Hoseini SM, Rajabiesterabadi H, Tarkhani R. Anaesthetic efficacy of eugenol on iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) in different size classes. Aquac Res. 2013; 46(2):1–08. https://doi.org/10.1111/are.12188
  » https://doi.org/10.1111/are.12188
• Jiang ZY, Woollard ACS, Wolff SP. Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. Lipids. 1991; 26(10):853–56. https://doi.org/10.1007/BF02536169
  » https://doi.org/10.1007/BF02536169
• Keen JH, Habig WH, Jakoby WB. Mechanism for several activities of glutathione-S-transferase. J Biol Chem. 1976; 251(20):6183–88. https://doi.org/10.1016/S0021-9258(20)81842-0
  » https://doi.org/10.1016/S0021-9258(20)81842-0
• Kiessling A, Johansson D, Zahl IH, Samuelsen OB. Pharmacokinetics, plasma cortisol and effectiveness of benzocaine, MS-222 and isoeugenol measured in individual dorsal aorta-cannulated Atlantic salmon (Salmo salar) following bath administration. Aquaculture. 2009; 286(3–4):301–08. https://doi.org/10.1016/j.aquaculture.2008.09.037
  » https://doi.org/10.1016/j.aquaculture.2008.09.037
• Lemos CH da P, Chung S, Ribeiro CVM, Copatti CE. Growth and biochemical variables in Amazon catfish (Pseudoplatystoma reticulatum♀ x Leiarius marmoratus♂) under different water pH. An Acad Bras Ciênc. 2018; 90(4):3573–81. http://dx.doi.org/10.1590/0001-3765201820180241
  » http://dx.doi.org/10.1590/0001-3765201820180241
• Limma-Netto JD, Sena AC, Copatti CE. Essential oils of Ocimum basilicum and Cymbopogon flexuosus in the sedation, anesthesia and recovery of tambacu (Piaractus mesopotamicus male x Colossoma macropomum female). Bol Inst Pesca. 2016; 42(3):727–33. http://dx.doi.org/10.20950/1678-2305.2016v42n3p727
  » http://dx.doi.org/10.20950/1678-2305.2016v42n3p727
• Lushchak VI. Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol. 2011; 101(1):13–30. https://doi.org/10.1016/j.aquatox.2010.10.006
  » https://doi.org/10.1016/j.aquatox.2010.10.006
• Lushchak OV, Kubrak OI, Storey JM, Storey KB, Lushchak VI. Low toxic herbicide Roundup induces mild oxidative stress in goldfish tissues. Chemosphere. 2009; 76(7):932–37. http://dx.doi.org/10.1016/j.chemosphere.2009.04.045
  » http://dx.doi.org/10.1016/j.chemosphere.2009.04.045
• McCord JE, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969; 244(22):6049–55. https://doi.org/10.1016/S0021-9258(18)63504-5
  » https://doi.org/10.1016/S0021-9258(18)63504-5
• McCormick SD, Regish A, O’Dea MF, Shrimpton JM. Are we missing a mineralocorticoid in teleost fish? Effects of cortisol, deoxycorticosterone and aldosterone on osmoregulation, gill Na+, K+-ATPase activity and isoform mRNA levels in Atlantic salmon. Gen Comp Endocrinol. 2008; 157(1):35–40. https://doi.org/10.1016/j.ygcen.2008.03.024
  » https://doi.org/10.1016/j.ygcen.2008.03.024
• Mirzargar SS, Mirghaed AT, Hoseini SM, Ghelichpour M, Shahbazi M, Yousefi M. Biochemical responses of common carp, Cyprinus carpio, to transportation in plastic bags using thymol as a sedative agent. Aquac Res. 2022; 53:191–98. https://doi.org/10.1111/are.15564
  » https://doi.org/10.1111/are.15564
• Nitz LF, Pellegrin L, Maltez LC, Pinto DSB, Monserrat JM, Sampaio LA, Garcia L. Temperature and hypoxia on oxidative stress responses in pacu Piaractus mesopotamicus J Therm Biol. 2020a; 92:102682. https://doi.org/10.1016/j.jtherbio.2020.102682
  » https://doi.org/10.1016/j.jtherbio.2020.102682
• Nitz LF, Pellegrin L, Pinto DSB, Maltez LC, Copatti CE, Garcia L. Secondary stress responses to hypoxia and reoxygenation at different temperatures in pacu (Piaractus mesopotamicus) juveniles. Aquac Res. 2020b; 51(11):4471–81. https://doi.org/10.1111/are.14792
  » https://doi.org/10.1111/are.14792
• Oliveira CPB, Lemos CHP, Felix e Silva A, De Souza SA, Albinati AC, Lima AO, Copatti CE. Use of eugenol for the anaesthesia and transportation of freshwater angelfish (Pterophyllum scalare). Aquaculture. 2019a; 513:734409. https://doi.org/10.1016/j.aquaculture.2019.734409
  » https://doi.org/10.1016/j.aquaculture.2019.734409
• Oliveira CPB, Lemos CHP, Vidal LVO, Couto RD, Pereira DSP, Copatti CE. Anaesthesia with eugenol in hybrid Amazon catfish (Pseudoplatystoma reticulatum × Leiarius marmoratus) handling: Biochemical and haematological responses. Aquaculture. 2019b; 501:255–59. https://doi.org/10.1016/j.aquaculture.2018.11.046
  » https://doi.org/10.1016/j.aquaculture.2018.11.046
• Oliveira IC, Oliveira RSM, Lemos CHP, Oliveira CPB, Felix e Silva A, Lorenzo VP, Lima AO, Cruz AL, Copatti CE. Essential oils from Cymbopogon citratus and Lippia sidoides in the anesthetic induction and transport of ornamental fish Pterophyllum scalare Fish Physiol Biochem. 2022; 48:501–19. https://doi.org/10.1007/s10695-022-01075-3
  » https://doi.org/10.1007/s10695-022-01075-3
• Pandey S, Parvez S, Sayeed I, Haque R, Bin-Hafeez B, Raisuddin S. Biomarkers of oxidative stress: A comparative study of river Yamuna fish Wallago attu (Bl. & Schn.). Sci Tot Environ. 2003; 309:105–15. https://doi.org/10.1016/S0048-9697(03)00006-8
  » https://doi.org/10.1016/S0048-9697(03)00006-8
• Parodi TV, Cunha MA, Becker AG, Zeppenfeld CC, Martins DI, Koakoski G, Barcellos LG, Heinzmann BM, Baldisserotto B. Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen Fish Physiol Biochem. 2014; 40:323–34. https://doi.org/10.1007/s10695-013-9845-z
  » https://doi.org/10.1007/s10695-013-9845-z
• Parodi TV, Santos CAD, Veronez A, Gomes LDC, Heinzmann BM, Baldisserotto B. Anesthetic induction and recovery time of Centropomus parallelus exposed to the essential oil of Aloysia triphylla Ciênc Rural. 2016; 46(12):2142–47. https://doi.org/10.1590/0103-8478cr20160039
  » https://doi.org/10.1590/0103-8478cr20160039
• Ramsay JM, Feist GW, Varga ZM, Westerfield M, Kent ML, Schreck CB. Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio Aquaculture. 2006; 258:565–74. https://doi.org/10.1016/j.aquaculture.2006.04.020
  » https://doi.org/10.1016/j.aquaculture.2006.04.020
• Rao VSN, Menezes AMS, Viana GSB. Effect of myrcene on nociception in mice. J Pharm Pharmacol. 1990; 42(12):877–78. https://doi.org/10.1111/j.2042-7158.1990.tb07046.x
  » https://doi.org/10.1111/j.2042-7158.1990.tb07046.x
• Readman GD, Owen SF, Knowles TG, Murrell JC. Species specific anaesthetics for fish anaesthesia and euthanasia. Sci Rep. 2017; 7:7102. https://doi.org/10.1038/s41598-017-06917-2
  » https://doi.org/10.1038/s41598-017-06917-2
• Ross LG, Ross B. Anaesthetic and sedative techniques for aquatic animals. John Wiley & Sons, Hoboken. 2009. https://doi.org/10.1002/9781444302264
  » https://doi.org/10.1002/9781444302264
• Salbego J, Toni C, Becker AG, Zeppenfeld CC, Menezes CC, Loro VL, Heinzmann BM, Baldisserotto B. Biochemical parameters of silver catfish (Rhamdia quelen) after transport with eugenol or essential oil of Lippia alba added to the water. Braz J Biol. 2017; 77(4):696–702. https://doi.org/10.1590/1519-6984.16515
  » https://doi.org/10.1590/1519-6984.16515
• Sena AC, Teixeira RR, Ferreira EL, Heinzmann BM, Baldisserotto B, Caron BO, Schmidt D, Couto RD, Copatti CE. Essential oil from Lippia alba has anaesthetic activity and is effective in reducing handling and transport stress in tambacu (Piaractus mesopotamicus × Colossoma macropomum). Aquaculture. 2016; 465:374–79. https://doi.org/10.1016/j.aquaculture.2016.09.033
  » https://doi.org/10.1016/j.aquaculture.2016.09.033
• Sepulchro LCOR, Carvalho MAG, Gomes LC. Salinity does not alter the effectiveness of menthol as an anesthetic and sedative during the handling and transport of juvenile fat snook (Centropomus parallelus). Braz J Biol. 2016; 76(3):757–63. https://doi.org/10.1590/1519-6984.04115
  » https://doi.org/10.1590/1519-6984.04115
• Sies H. Oxidative stress: Oxidants and antioxidants. Exp Physiol. 1997; 82(2):291–95. https://doi.org/10.1113/expphysiol.1997.sp004024
  » https://doi.org/10.1113/expphysiol.1997.sp004024
• Simões LN, Medeiros LCC, Heinzmann BN, Loro VL, Gomes LC, Silva DT, Schmidt D, Baldisserotto B. Essential oil of Lippia alba as a sedative and anesthetic for the sea urchin Echinometra lucunter (Linnaeus, 1758). Mar Freshw Behav Physiol. 2017; 50(3):205–17. https://doi.org/10.1080/10236244.2017.1362317
  » https://doi.org/10.1080/10236244.2017.1362317
• Sink TD, Kumaran S, Lochmann RT. Development of a whole-body cortisol extraction procedure for determination of stress in golden shiners, Notemigonus crysoleucas Fish Physiol Biochem. 2007; 33:189–93. https://doi.org/10.1007/s10695-007-9130-0
  » https://doi.org/10.1007/s10695-007-9130-0
• Small BC. Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methanesulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus Aquaculture. 2003; 218:177–85. https://doi.org/10.1016/S0044-8486(02)00302-2
  » https://doi.org/10.1016/S0044-8486(02)00302-2
• Sneddon LU. Clinical anesthesia and analgesia in fish. J Exot Pet Med. 2012; 21(1):32–43. https://doi.org/10.1053/j.jepm.2011.11.009
  » https://doi.org/10.1053/j.jepm.2011.11.009
• Souza CF, Baldissera MD, Bianchini AE, Silva EG, Mourão RHV, Silva LFV, Schmidt D, Heinzmann BM, Baldisserotto B. Citral and linalool chemotypes of Lippia alba essential oil as anesthetics for fish: A detailed physiological analysis of side effects during anesthetic recovery in silver catfish (Rhamdia quelen). Fish Physiol Biochem. 2018; 44:21–34. https://doi.org/10.1007/s10695-017-0410-z
  » https://doi.org/10.1007/s10695-017-0410-z
• Tarkhani R, Imani A, Jamali H, Moghanlou KS. Anesthetic efficacy of eugenol on flowerhorn (Amphilophus labiatus x Amphilophus trimaculatus). Aquac Res. 2016; 48(6):3207–15. https://doi.org/10.1111/are.13151
  » https://doi.org/10.1111/are.13151
• Teixeira RR, Souza RC, Sena AC, Baldisserotto B, Heinzmann BM, Copatti CE. Essential oil of Aloysia triphylla is effective in Nile tilapia transport. Bol Inst Pesca. 2018; 44(1):17–24. https://doi.org/10.20950/1678-2305.2018.263
  » https://doi.org/10.20950/1678-2305.2018.263
• Teixeira RR, Souza RC, Sena AC, Baldisserotto B, Heinzmann BM, Couto RD, Copatti CE. Essential oil of Aloysia triphylla in Nile tilapia: Anaesthesia, stress parameters and sensory evaluation of fillets. Aquac Res. 2017; 48(7):3383–92. https://doi.org/10.1111/are.13165
  » https://doi.org/10.1111/are.13165
• Tondolo JSM, Amaral LP, Simões LN, Garlet QI, Schindler B, Oliveira TM, Silva BF, Gomes LC, Baldisserotto B, Mallmann CA, Heinzmann BM. Anesthesia and transport of fat snook Centropomus parallelus with essential oil of Nectandra megapotamica (Spreng.) Mez. Neotrop Ichthyol. 2013; 11(3):667–74. http://dx.doi.org/10.1590/S1679-6225201300030002
  » http://dx.doi.org/10.1590/S1679-6225201300030002
• Toni C, Martos-Sitcha JA, Baldisserotto B, Heinzmann BM, Silva LD, Martinez-Rodriguez G, Mancera JM. Sedative effect of 2-phenoxyethanol and essential oil of Lippia alba on stress response in gilthead sea bream (Sparus aurata). Res Vet Sci. 2015; 103:20–27. https://doi.org/10.1016/j.rvsc.2015.09.006
  » https://doi.org/10.1016/j.rvsc.2015.09.006
• Tsuzuki MY, Sugai JK, Maciel JC, Francisco CJ, Cerqueira VR. Survival, growth and digestive enzyme activity of juveniles of the fat Snook (Centropomus parallelus) reared at different salinities. Aquaculture. 2007; 271:319–25. https://doi.org/10.1016/j.aquaculture.2007.05.002
  » https://doi.org/10.1016/j.aquaculture.2007.05.002
• Wendelaar Bonga SE. The stress response in fish. Physiol Rev. 1997; 77(3):591–625. https://doi.org/10.1152/physrev.1997.77.3.591
  » https://doi.org/10.1152/physrev.1997.77.3.591
• Wosnick N, Bendhack F, Leite RD, Morais RN, Freire CA. Benzocaine-induced stress in the euryhaline teleost, Centropomus parallelus and its implications for anesthesia protocols. Comp Biochem Physiol. A. 2018; 226:32–37. https://doi.org/10.1016/j.cbpa.2018.07.021
  » https://doi.org/10.1016/j.cbpa.2018.07.021

ADDITIONAL NOTES

• HOW TO CITE THIS ARTICLE

  Simões-Bueno LN, Copatti CE, Gomes LC, Val AL, Amanajás RD, Caron BO, Heinzmann BM, Baldisserotto B. Linalool chemotype essential oil from Lippia alba in the anesthesia of fat snook (Centropomus parallelus): ventilatory rate, biochemical, antioxidant, and oxidative status parameters. Neotrop Ichthyol. 2024; 22(1):e230114. https://doi.org/10.1590/1982-0224-2023-0114

Edited-by

Osmar Luiz

Publication Dates

• Publication in this collection
  19 Apr 2024
• Date of issue
  2024

History

• Received
  17 Oct 2023
• Accepted
  25 Jan 2024