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Survey of major weed problems, management practices and herbicide use in extensive row crops from Argentina

Abstract:

Background:

Weeds pose a significant challenge to Argentine crops, leading to herbicide resistance and environmental concerns.

Objective:

To identify key weed problems, assess management practices, and herbicide use.

Methods:

A web-based survey system, with closed questions, was distributed from March to August 2020 among 147 agricultural stakeholders related to soybean, corn, wheat, and sunflower crops in Argentina.

Results:

Notable troublesome weeds included Conyza bonariensis (L.) Cronq., Digitaria sanguinalis (L.) Scop., Eleusine indica (L.) Gaertn., Amaranthus hybridus L., and Sorghum halepense (L.) Pers. Herbicide usage was as follows: 86% used burndown fallow chemical control, 51% adhered to labeled application rates, 40% applied herbicides at the labeled growth stage, and 37% rotated modes of action. Non-chemical controls were also employed, with 67% favoring crop rotation and 36% focused on preventing weed seed production. Glyphosate was the dominant herbicide, employed by over 90% of respondents. For summer crops, frequently used herbicides included paraquat, 2,4-D (80% of respondents), atrazine (60% of respondents), sulfentrazone, flumioxazin, S-metolachlor (60% of respondents), and clethodim (65% of respondents). In winter cereals, 2,4-D, flurochloridone, flumioxazin, and pyroxasulfone were the top choices.

Conclusions:

This survey underscores the high reliance on chemical control in Argentina's major crops. The findings provide crucial insights for regional policy planning, emphasizing the importance of integrating diverse weed management tactics. It also highlights the need for proactive integrated management strategies at the field level to mitigate and prevent weed issues in Argentina, offering a valuable approach for analyzing weed problems in extensive crops worldwide.

Keywords:
Chemical Control; Integrated Weed Management; Problematic Weeds; Spring-Summer Crops; Winter Cereals

1. Introduction

Crop production in no-till (NT) systems has increased in Argentina since the mid-1990s. This increase can be attributed to the rapid adoption of glyphosate-resistant (GR) soybean varieties. This system simplifies weed management, increases crop yields, reduces weed control costs by approximately four times compared to conventional systems (Scursoni, Satorre, 2010Scursoni JA, Satorre EH. Glyphosate management strategies, weed diversity and soybean yield in Argentina. Crop Prot. 2010;29(9):957-62. Available from: https://doi.org/10.1016/j.cropro.2010.05.001
https://doi.org/10.1016/j.cropro.2010.05...
; Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
a) and lowers fuel and labor costs (Qaim, Traxler, 2005Qaim M, Traxler G. Roundup ready soybeans in Argentina: farm level and aggregate welfare effects. Agric Econ. 2005;32(1):73-86. Available from: https://doi.org/10.1111/j.0169-5150.2005.00006.x
https://doi.org/10.1111/j.0169-5150.2005...
). No-tillage systems also offer benefits such as soil water conservation and soil erosion reduction (Peiretti, 1999Peiretti R. The development and future of direct seed cropping systems in Argentina. In: Proceedings of 10th International Soil Conservation Organization Meeting; West Lafayette, USA. West Lafayette: International Soil Conservation; 1999[access January 25, 2023]. Available from: https://topsoil.nserl.purdue.edu/nserlweb-old/isco99/pdf/iscodisc/Sustaining%20the%20Global%20Farm/K002-Peiretti.pdf
https://topsoil.nserl.purdue.edu/nserlwe...
; Viglizzo et al., 2010), making soybean and corn production profitable not only in traditional cropping areas but also in marginal areas.

Simultaneously with the increased adoption of GR crops, crop rotations mainly consisted of soybean monoculture, soybean/corn, or wheat/late soybean-soybean (Fuente et al., 2021aFuente EB, Suárez SA, Lenardis AE, Oreja FH, Torcat-Fuentes M. [Changes in weed communities in the maize crops of the rolling pampa (Argentina) between 1960 and 2019]. Agron Amb. 2021a;41(2):169-78. Spanish.). These changes in production systems heightened reliance on glyphosate, leading to its multiple applications within the same crop (Vila-Aiub et al., 2007Vila-Aiub MM, Balbi MC, Gundel PE, Ghersa CM, Powles SB. Evolution of glyphosate-resistant johnsongrass (Sorghum halepense) in glyphosate-resistant soybean. Weed Sci. 2007;55(6):566-71. Available from: https://doi.org/10.1614/WS-07-053.1
https://doi.org/10.1614/WS-07-053.1...
). The high and intense selection pressure on glyphosate-susceptible species led to a shift in the weed community from glyphosate-susceptible to GR (Young, 2006Young BG. Changes in herbicide use patterns and production practices resulting from glyphosate-resistant crops. Weed Technol. 2006;20(2):301-7. Available from: https://doi.org/10.1614/WT-04-189.1
https://doi.org/10.1614/WT-04-189.1...
; Ryan et al., 2010Ryan MR, Smith RG, Mirsky SB, Mortensen DA, Seidel R. Management filters and species traits: weed community assembly in long-term organic and conventional systems. Weed Sci. 2010;58(3):265-77. Available from: https://doi.org/10.1614/WS-D-09-00054.1
https://doi.org/10.1614/WS-D-09-00054.1...
). The consequences of this oversimplification of rotations in Argentinean agroecosystems resulted in a loss of biodiversity among weedy plants in primary cropping lands, with only a few challenging-to-control species remaining (Fuente et al., 2006Fuente EB, Suarez SA, Ghersa CM. Soybean weed community composition and richness between 1995 and 2003 in the Rolling Pampas (Argentina). Agric Ecosyst Environ. 2006;115(1-4):229-36. Available from: https://doi.org/10.1016/j.agee.2006.01.009
https://doi.org/10.1016/j.agee.2006.01.0...
; 2021aFuente EB, Suárez SA, Lenardis AE, Oreja FH, Torcat-Fuentes M. [Changes in weed communities in the maize crops of the rolling pampa (Argentina) between 1960 and 2019]. Agron Amb. 2021a;41(2):169-78. Spanish.) and an increasing number of GR biotypes since 2005 (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
; Asociación Argentina de Productores en Siembra Directa, 2023Asociación Argentina de Productores en Siembra Directa – Aapresid. [Aapresid REM: weeds]. Rosario: Asociación Argentina de productores en Siembra Directa; 2023[access Jan 10, 2023]. Spanish. Available from: https://www.aapresid.org.ar/rem/malezas
https://www.aapresid.org.ar/rem/malezas...
; Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
). According to results obtained in a previous survey distributed to producers and crop consultants, among the top 15 most challenging weeds to manage, 11 were glyphosate-tolerant species or GR biotypes (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). The difficulty to manage tolerant and herbicide-resistant weeds is leading producers to increase the use of additional herbicides, in many cases increasing toxicity and environmental risks compared with glyphosate-based herbicide programs (Kniss, 2017Kniss AR. Long-term trends in the intensity and relative toxicity of herbicide use. Nat Comm. 2017;8:1-7. Available from: https://doi.org/10.1038/ncomms14865
https://doi.org/10.1038/ncomms14865...
). Nevertheless, there is a growing awareness of this issue among producers (Rodriguez et al., 2019Rodriguez S, Kruk BC, Satorre EH. [Farmer's perception of the importance of biotic adversities of extensive grain crops in the Pampas Region]. Agron Amb. 2019;39(1):16-25. Spanish.), and the adoption of integrated weed management (IWM) programs is increasing (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). Argentinean producers and consultants surveyed reported using crop rotation, row spacing, competitive crop cultivars, and planting dates as their strategies (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). Among the best management practices (BMP) aimed at reducing the risks of herbicide resistance, 75% to 95% of the surveyed producers and consultants in Argentina indicated that the rotation of multiple modes of action, following labeled herbicide recommendations (including rates and plant sizes), and managing weed seed production were the most commonly utilized BMPs (Norsworthy et al., 2012Norsworthy JK, Ward SM, Shaw DR, Llewellyn RS, Nichols RL, Webster TM et al. Reducing the risks of herbicide resistance: best management practices and recommendations. Weed Sci. 2012;60(SP1):31-62. Available from: https://doi.org/10.1614/WS-D-11-00155.1
https://doi.org/10.1614/WS-D-11-00155.1...
; Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). Since the last survey reported by Scursoni et al. (2019)Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
, some crops such as corn, wheat and sunflower increased their planted areas, by 35, 14 and 7%, respectively, while soybean area was reduced 13% (Bolsa Cereales, 2024Bolsa Cereales. [Reports and data]. Buenos Aires: Bolsa de Cereales; 2024[access May 1, 2024]. Spanish. Available from: https://www.bolsadecereales.com/estimaciones-informes
https://www.bolsadecereales.com/estimaci...
). These changes in the crop planted areas may have changed as well weed management decisions and resulting weed communities.

The growth of herbicide-resistant weeds poses enormous challenges for the sustainability of production systems. While significant efforts are being made in weed management at the field level, the influence of regional production context on the presence of herbicide-resistant weeds remains unknown (Garibaldi et al., 2023Garibaldi LA, Goldenberg MG, Burian A, Santibañez F, Satorre EH, Martini GD et al. Smaller agricultural fields, more edges, and natural habitats reduce herbicide-resistant weeds. Agric Ecosys Environ. 2023;342. Available from: https://doi.org/10.1016/j.agee.2022.108260
https://doi.org/10.1016/j.agee.2022.1082...
). Despite the continuous confirmation of herbicide-resistant weed biotypes in Argentina (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
; Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
), there is a positive trend towards the adoption of IWM programs (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). Some IWM practices have seen increased adoption, such as the use of cover crops (Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales, 2022Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
), the intensification and diversification of crop rotations (Fuente et al., 2021bFuente EB, Oreja FH, Lenardis AE, Fuentes MT, Agosti B, Barrio A et al. Intensification of crop rotation affecting weed communities and the use of herbicides in the rolling Pampa. Heliyon. 2021b;7(1):1-13. Available from: https://doi.org/10.1016/j.heliyon.2021.e06089
https://doi.org/10.1016/j.heliyon.2021.e...
), and the implementation of targeted herbicide applications (Arditi et al., 2023Arditi AB, Camio MI, Velazquez L, Errandosoro F. Early adoption of Industry 4.0 technologies in the agricultural sector: a phenomenological analysis. J Int Concil Small Buss Manag. 2023;4(3):1-28. Available from: https://doi.org/10.1080/26437015.2023.2201894
https://doi.org/10.1080/26437015.2023.22...
).

Given the dynamic nature of crop management practices, there has been limited documentation of recent developments in the adoption of IWM practices and herbicide use in Argentina. Despite increased interest in the environmental and economic impacts of monocultures, reports on soybean monoculture have been scarce and based mainly on the observation of area changes at the regional level (Barros et al., 2015Barros V, Vera C, Agosta E, Araneo D, Camilloni I, Carril A et al. [Third national communication]. Buenos Aires: Jefatura de Gabinete de Ministros; 2015. Spanish.; Duval et al., 2015Duval ME, Matías E, Capurro JE, Galantini JA, Andriani JM. Use of cover crops in soybean monoculture: effects on water and carbon balance. Cienc Suelo. 2015;33(2):247-61.). In the past, information on Argentinean harvested area or crop yield was only available at the regional level and the occurrence of monoculture was based on this type of information for many years (Barros et al., 2015Barros V, Vera C, Agosta E, Araneo D, Camilloni I, Carril A et al. [Third national communication]. Buenos Aires: Jefatura de Gabinete de Ministros; 2015. Spanish.; Duval et al., 2015Duval ME, Matías E, Capurro JE, Galantini JA, Andriani JM. Use of cover crops in soybean monoculture: effects on water and carbon balance. Cienc Suelo. 2015;33(2):247-61.; Sly, 2017Sly MJH. The Argentine portion of the soybean commodity chain. Palgrave Comm. 2017;3:1-11. Available from: https://doi.org/10.1057/palcomms.2017.95
https://doi.org/10.1057/palcomms.2017.95...
). Recently, high-resolution maps of annual crop were generated in Argentina, allowing the characterization of the spatial distribution of crops in large regions, and the quantification of crop rotation and monoculture at the regional level (Abelleyra et al., 2019Abelleyra D, Banchero S, Verón S, Mosciaro MJ, Volante J, Boasso M et al., 2019. [Argentina national map of crops 2018/2019]. Buenos Aires: Instituto Nacional de Tecnologia de Agropecuária; 2019[access July 5, 2023]. Spanish. Available from: https://doi.org/10.5281/zenodo.8286326
https://doi.org/10.5281/zenodo.8286326...
; Abelleyra et al., 2020Abelleyra D, Verón S, Banchero S, Mosciaro MJ, Franzoni A, Boasso M et al. [Argentina national map of crops 2019/2020]. Buenos Aires: Instituto Nacional de Tecnologia de Agropecuária; 2020[access April 3, 2023]. Spanish. Available from: https://doi.org/10.5281/zenodo.8286310
https://doi.org/10.5281/zenodo.8286310...
; Abelleyra et al., 2021Abelleyra D, Verón S, Banchero S, Iturralde Elortegui MR, Valiente S, Puig O et al. [Argentina national map of crops 2020/2021]. Buenos Aires: Instituto Nacional de Tecnologia de Agropecuária; 2021[access March 14, 2023]. Spanish. Available from: https://doi.org/10.5281/zenodo.8286318
https://doi.org/10.5281/zenodo.8286318...
; Abelleyra et al., 2022Abelleyra D, Verón S, Banchero S, Iturralde Elortegui MR, Zelaya K, Murray F et al. [Argentina national map of crops 2021/2022]. Buenos Aires: Instituto Nacional de Tecnologia de Agropecuária; 2022[access March 14, 2023]. Spanish. Available from: https://doi.org/10.5281/zenodo.8284081
https://doi.org/10.5281/zenodo.8284081...
). Given that agricultural landscapes are tending towards simplification, larger plots and monocultures, species resistant to herbicides may increase compared to more diverse and complex landscapes (Abelleyra et al., 2024Abelleyra D, Banchero S, Verón S. Characterization of crop sequences in Argentina: spatial distribution and determinants. SSRN. 2024. Available from: https://doi.org/10.2139/ssrn.4725018
https://doi.org/10.2139/ssrn.4725018...
). For example, resistance-inducing mutations are often related to fitness costs under non-herbicide-treated conditions (Vila-Aiub, 2019Vila-Aiub MM. Fitness of herbicide-resistant weeds: current knowledge and implications for management. Plants 2019;8(11):1-11. Available from: https://doi.org/10.3390/plants8110469
https://doi.org/10.3390/plants8110469...
). More diverse and complex weed communities could promote genetic variation of weeds within the crop field with those found outside in other fields and thus reduce the spread of herbicide-resistant traits. As weed community composition within a crop field changes with distance from the field edge (Bourgeois et al., 2020Bourgeois B, Gaba S, Plumejeaud C, Bretagnolle V. Weed diversity is driven by complex interplay between multi-scale dispersal and local filtering. Proc Biol Sci. 2020;287(1930):1-10. Available from: https://doi.org/10.1098/rspb.2020.1118
https://doi.org/10.1098/rspb.2020.1118...
), plots will encounter large natural and semi-natural habitats that can function as barriers to the spread of herbicide-resistant traits.

Surveys that evaluate management practices are important tools to monitor the impact on weed communities (Norsworthy et al., 2012Norsworthy JK, Ward SM, Shaw DR, Llewellyn RS, Nichols RL, Webster TM et al. Reducing the risks of herbicide resistance: best management practices and recommendations. Weed Sci. 2012;60(SP1):31-62. Available from: https://doi.org/10.1614/WS-D-11-00155.1
https://doi.org/10.1614/WS-D-11-00155.1...
). Producers and consultants have information about the main problematic weed species and the strategies applied to different situations in their respective regions (Riar et al., 2013Riar DS, Norsworthy JK, Steckel LE, Stephenson DO IV, Eubank TW, Bond JA et al. Adoption of best management practices for herbicide-resistant weeds in midsouthern United States cotton, rice and soybean. Weed Technol. 2013;27(4):788-97. Available from: https://doi.org/10.1614/WT-D-13-00087.1
https://doi.org/10.1614/WT-D-13-00087.1...
; Schwartz-Lazaro et al., 2021). However, information about weed management and herbicide use covering the main crop producing provinces in Argentina is scarce. The information derived from surveys will allow to provide information of the current weed management practices and issues in Argentinean crop production and future research needs to be based on producer concerns. Given that weed management decisions are dynamics and weed communities change continuously, we proposed to conduct a new survey to determine i) the major weed problems, ii) the adoption of weed management practices and iii) the use of herbicides in the main crops in Argentina.

2. Materials and Methods

A web-based survey system containing eight questions was distributed from March to August 2020 among agricultural stakeholders in Argentina. One hundred and forty-seven surveys were completed by producers and consultants from the main crop production areas in Argentina, which include the provinces of Buenos Aires, Chaco, Córdoba, Entre Ríos, La Pampa, Salta, San Luis, Santa Fe, Santiago del Estero, and Tucumán. The survey covered the location and the most important crops in Argentina, including soybean, corn, sunflower, and wheat, for each respondent. For questions 4 to 7, a list of predefined answers (Table 1) was provided, allowing respondents to select none, one, or more options for: i) the most problematic weed species per field and crop; ii) the frequency of use (always, often, sometimes, or never) of each farming practice; and iii) the frequency of use (always, often, sometimes, or never) of each herbicide (Table 2).

Table 1
The first part of the questionnaire included in the survey
Table 2
Second part of the questionnaire included in the survey

When considering species as the most troublesome to manage, the ranking of the most problematic weeds for each area was determined based on the number of times each weed was mentioned. Additionally, the ranking of IWM practices and herbicides used in summer crops (soybean, corn, and sunflower) and winter crops (wheat) was determined by considering the frequency of each practice. Responses to multiple-choice questions were converted into percentages and analyzed using descriptive statistics. Absolute frequency was used to determine the number cases of most problematic weeds reported by respondents, and the relative frequency of farming practices and frequency of herbicide was expressed in percentages.

3. Results and Discussion

3.1 Characterization of respondents

According to the survey, 84% of respondents worked in the most important agricultural provinces in Argentina (Figure 1). Among the respondents, 98% were involved in the production of soybeans or corn, 89% in wheat production, and 45% in sunflower production. Approximately 54% to 64% of respondents managed more than 500 hectares of corn, wheat, and soybeans, while 14% to 23% managed 300 to 500 hectares. Around 9% managed 100 to 300 hectares, and 6% to 12% managed less than 100 hectares (Figure 2).

Figure 1
Percentage of respondents of the survey from each province of Argentina
Figure 2
Area managed (%) for each crop grouped according to farm size

3.2 Troublesome weeds

According to the survey, the most problematic weed species included hairy fleabane [Conyza bonariensis (L.) Cronquist], large crabgrass [Digitaria sanguinalis (L.) Scop.], goosegrass [Eleusine indica (L.) Gaertn.], smooth pigweed (Amaranthus hybridus L.), johnsongrass [Sorghum halepense (L.) Pers.], pigweed (Amaranthus sp.), hairy fleabane, (Conyza sp.), Chloris sp., erect dayflower (Commelina erecta L.) and fleabane [Conyza sumatrensis (Retz.) E. Walker] (Figure 3). All of these species have been reported as GR biotypes in Argentina (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
; Asociación Argentina de Productores en Siembra Directa, 2023Asociación Argentina de Productores en Siembra Directa – Aapresid. [Aapresid REM: weeds]. Rosario: Asociación Argentina de productores en Siembra Directa; 2023[access Jan 10, 2023]. Spanish. Available from: https://www.aapresid.org.ar/rem/malezas
https://www.aapresid.org.ar/rem/malezas...
; Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
). Sorghum halepense was the first GR weed registered in Argentina (Vila-Aiub et al., 2007Vila-Aiub MM, Balbi MC, Gundel PE, Ghersa CM, Powles SB. Evolution of glyphosate-resistant johnsongrass (Sorghum halepense) in glyphosate-resistant soybean. Weed Sci. 2007;55(6):566-71. Available from: https://doi.org/10.1614/WS-07-053.1
https://doi.org/10.1614/WS-07-053.1...
) followed by resistance to clethodim and haloxyfop-methyl (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
). Digitaria sanguinalis (Yanniccari et al., 2022Yanniccari M, Vázquez-García JG, Gigón R, Palma-Bautista C, Vila-Aiub M, Prado R. A novel EPSPS Pro-106-His mutation confers the first case of glyphosate resistance in Digitaria sanguinalis. Pest Manag Sci. 2022;78(7):3135-43. Available from: https://doi.org/10.1002/ps.6940
https://doi.org/10.1002/ps.6940...
), E. indica (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
), C. bonariensis (Puricelli et al., 2015Puricelli ECJM, Faccini DE, Metzler M, Torres PS. Differential susceptibility of Conyza bonariensis biotypes to glyphosate and ALS-inhibiting herbicides in Argentina. Agric Sci. 2015;6(1):22-30. Available from: https://doi.org/10.4236/as.2015.61003
https://doi.org/10.4236/as.2015.61003...
) and C. sumatrensis biotypes were also identified as GR (Balassone et al., 2020Balassone F, Puricelli E, Faccini D. [Sensitivity of Conyza sumatrensis biotypes to glyphosate and ALS-inhibiting herbicides at two growth stages]. Agriscientia. 2020;37(2):11-20. Spanish. Available from: https://doi.org/10.31047/1668.298x.v37.n2.25404
https://doi.org/10.31047/1668.298x.v37.n...
). A biotype of C. sumatrensis resistant to acetolactate synthase (ALS)-inhibiting herbicides (Group 2) was also reported by Balassone et al. (2021)Balassone F, Tuesca D, Puricelli E, Faccini D. [Black shoot (Conyza sumatrensis (Retz.) E. Walker) resistant to ALS inhibitors in southern Santa Fe]. In Proceedings of the 3rd Argentine National Weed Congress; Buenos Aires, Argentina. Buenos Aires: Argentine Association of Weed Science; 2021[access April 12, 2023]. Spanish. Available from: http://www.asacim.org.ar/publicaciones/
http://www.asacim.org.ar/publicaciones/...
. The genus Amaranthus has the highest number of herbicide-resistant biotypes reported in Argentina, with the majority being A. hybridus biotypes and among them, GR biotypes (Bracamonte et al., 2018Bracamonte E, Portugal J, Bellucini P, Alcántara-de la Cruz R, Rojano-Delgado AM, Salvidia EA et al. [Resistance of Amaranthus hybridus L. to EPSPS and ALS herbicides and alternative chemical management in the central region of Córdoba]. In Proceedings of the 3rd Argentine National Weed Congress; Buenos Aires, Argentina. Buenos Aires: Argentine Association of Weed Science; 2018[access April 7, 2023.]. Spanish. Available from: http://www.asacim.org.ar/publicaciones/
http://www.asacim.org.ar/publicaciones/...
; Dellaferrera et al., 2018Dellaferrera I, Cortés E, Panigo E, Prado R, Christoffoleti P, Perreta M. First report of Amaranthus hybridus with multiple resistance to 2, 4-D, dicamba, and glyphosate. Agronomy. 2018;8(8):1-8. Available from: https://doi.org/10.3390/agronomy8080140
https://doi.org/10.3390/agronomy8080140...
; Perotti et al., 2018Perotti VE, Larran AS, Palmieri VE, Martinatto AK, Alvarez CE, Tuesca D et al. A novel triple amino acid substitution in the EPSPS found in a high-level glyphosate-resistant Amaranthus hybridus population from Argentina. Pest Manag Sci. 2019;75(5):1242-51. Available from: https://doi.org/10.1002/ps.5303
https://doi.org/10.1002/ps.5303...
; García et al., 2019García MJ, Palma-Bautista C, Rojano-Delgado AM, Bracamonte E, Portugal J, Alcántara-de la Cruz R et al. The triple amino acid substitution TAP-IVS in the EPSPS gene confers high glyphosate resistance to the superweed Amaranthus hybridus. Int J Mol Sci. 2019;20(10):1-15. Available from: https://doi.org/10.3390/ijms20102396
https://doi.org/10.3390/ijms20102396...
) but also, to other herbicides such as imazethapyr, chlorimuron, 2,4-D and dicamba (Tuesca, Nisensohn, 2001Tuesca D, Nisensohn L. [Resistance of Amaranthus quitensis to imazethapyr and clhorimuron-ethyl]. Pesq Agropec Bras. 2001;36(4):601-6. Spanish. Available from: https://doi.org/10.1590/S0100-204X2001000400002
https://doi.org/10.1590/S0100-204X200100...
; Bracamonte et al., 2018Bracamonte E, Portugal J, Bellucini P, Alcántara-de la Cruz R, Rojano-Delgado AM, Salvidia EA et al. [Resistance of Amaranthus hybridus L. to EPSPS and ALS herbicides and alternative chemical management in the central region of Córdoba]. In Proceedings of the 3rd Argentine National Weed Congress; Buenos Aires, Argentina. Buenos Aires: Argentine Association of Weed Science; 2018[access April 7, 2023.]. Spanish. Available from: http://www.asacim.org.ar/publicaciones/
http://www.asacim.org.ar/publicaciones/...
; Dellaferrera et al., 2018Dellaferrera I, Cortés E, Panigo E, Prado R, Christoffoleti P, Perreta M. First report of Amaranthus hybridus with multiple resistance to 2, 4-D, dicamba, and glyphosate. Agronomy. 2018;8(8):1-8. Available from: https://doi.org/10.3390/agronomy8080140
https://doi.org/10.3390/agronomy8080140...
). Recent evaluations of over 50 populations from the central area of Argentina revealed that more than 80% of the assessed Amaranthus biotypes were GR. Among them, 71% showed resistance to topramezone (a 4-hydroxylphenylpyruvate dioxygenase [HPPD]-inhibiting herbicide) (Group 27), and 56% were resistant to fomesafen (a protoporphyrinogen oxidase [PPO]-inhibiting herbicide) (Group 14) (Scursoni et al., 2022Scursoni JA, Tuesca D, Balassone F, Morello JP, Medina Herrera D, Lescano MC et al. Response of smooth pigweed (Amaranthus hybridus) accessions from Argentina to herbicides from multiple sites of action. Weed Technol. 2022;36(3):384-9. Available from: https://doi.org/10.1017/wet.2022.9
https://doi.org/10.1017/wet.2022.9...
). Additionally, in Argentina, Palmer amaranth (Amaranthus palmeri S. Watson) populations resistant to glyphosate and other herbicides such as imazethapyr, imazapic, chlorimuron, nicosulfuron, and diclosulam have been identified (Berger et al., 2016Berger S, Madeira PT, Ferrell J, Gettys L, Morichetti S, Cantero JJ et al. Palmer amaranth (Amaranthus palmeri) identification and documentation of ALS-resistance in Argentina. Weed Sci. 2016;64(2):312-20. Available from: https://doi.org/10.1614/WS-D-15-00125.1
https://doi.org/10.1614/WS-D-15-00125.1...
; Larran et al., 2017Larran AS, Palmieri VE, Perotti VE, Lieber L, Tuesca D, Permingeat HR. Target-site resistance to acetolactate synthase (ALS)-inhibiting herbicides in Amaranthus palmeri from Argentina. Pest Manag Sci. 2017;73(12):2578-84. Available from: https://doi.org/10.1002/ps.4662
https://doi.org/10.1002/ps.4662...
; Palma-Bautista et al., 2019Palma-Bautista C, Torra J, Garcia MJ, Bracamonte E, Rojano-Delgado AM, Alcántara-de la Cruz R et al. Reduced absorption and impaired translocation endows glyphosate resistance in Amaranthus palmeri harvested in glyphosate-resistant soybean from Argentina. J Agric Food Chem. 2019;67(4):1052-60. Available from: https://doi.org/10.1021/acs.jafc.8b06105
https://doi.org/10.1021/acs.jafc.8b06105...
; Kaundun et al., 2019Kaundun SS, Jackson LV, Hutchings SJ, Galloway J, Marchegiani E, Howell A et al. Evolution of target-site resistance to glyphosate in an Amaranthus palmeri population from Argentina and its expression at different plant growth temperatures. Plants. 2019;8(11):1-21. Available from: https://doi.org/10.3390/plants8110512
https://doi.org/10.3390/plants8110512...
).

Figure 3
Ranking of weed species in the surveyed agricultural area of Argentina, considering the number of informed cases. In cases without species identification, only the genus was registered

The list of species was similar to that reported by Scursoni et al. (2019)Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
; however, the weed ranking changed. D. sanguinalis rose from 8th place in Scursoni et al. (2019)Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
to the 2nd place in the present survey, confirming the difficulty of managing of this weed with herbicides due to a long emergence period (Oreja et al., 2020Oreja FH, Batlla D, Fuente EB. Digitaria sanguinalis seed dormancy release and seedling emergence are affected by crop canopy and stubble. Weed Res. 2020;60(2):111-20. Available from: https://doi.org/10.1111/wre.12392
https://doi.org/10.1111/wre.12392...
). Lolium sp. decreased from 7th to the 16th place on the present survey, possibly due to the increased use of acetyl-CoA carboxylase–(ACCase)-inhibiting (Group 1) herbicides to control this species (Yanniccari, Gigon, 2020Yanniccari M, Gigón R. Cross-resistance to acetyl-CoA carboxylase–inhibiting herbicides conferred by a target-site mutation in perennial ryegrass (Lolium perenne) from Argentina. Weed Sci. 2020;68(2):116-24. Available from: https://doi.org/10.1017/wsc.2020.1
https://doi.org/10.1017/wsc.2020.1...
), especially in the fallow period before summer crops (Figures 5 and 6). However, the repetitive use of these herbicides is selecting for herbicide-resistant populations (Yanniccari, Gigon, 2020Yanniccari M, Gigón R. Cross-resistance to acetyl-CoA carboxylase–inhibiting herbicides conferred by a target-site mutation in perennial ryegrass (Lolium perenne) from Argentina. Weed Sci. 2020;68(2):116-24. Available from: https://doi.org/10.1017/wsc.2020.1
https://doi.org/10.1017/wsc.2020.1...
). Commelina erecta, a weed that is challenging to control in NT systems due to glyphosate tolerance (Panigo et al., 2012Panigo ES, Dellaferrera IM, Acosta JM, Bender AG, Garetto JI, Perreta MG. Glyphosate-induced structural variations in Commelina erecta L.(Commelinaceae). Ecotoxicol Environ Saf. 2012;76:135-42. Available from: https://doi.org/10.1016/j.ecoenv.2011.10.002
https://doi.org/10.1016/j.ecoenv.2011.10...
) and perennial lifecycle; requiring long-term management instead of single applications, went up from 15th to 9th place in the present survey. The present ranking of the main winter species was: Sonchus oleraceus L. and Conyza sp. (including C. sumatrensis), the latter is a facultative winter species problematic in summer crops (Figure 3). The increasing importance of S. oleraceus importance as a problematic species highlights the growing challenges in its control (Daita et al., 2021Daita F, Gigena PD, Lucero MA, Mulko JA. [Control of Sonchus oleraceus L. "stewweed" with post-emergence herbicides in fallow land]. In: Proceedings of the 3rd Argentine National Weed Congress. Buenos Aires, Argentina. Buenos Aires: Argentine Association of Weed Science; 2021[access April 5, 2023]. Spanish. Available from: http://www.asacim.org.ar/publicaciones/
http://www.asacim.org.ar/publicaciones/...
), however there are no HR biotypes identified in Argentina so far (Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
).

3.3 IWM practices adoption

Compared with the previous survey (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
), the use of IWM practices has increased. Among the most adopted strategies, herbicides are still more adopted than non-chemical weed management practices (Figure 4). Chemical burndown during the fallow period, periodic monitoring of weeds and crop rotation were the most adopted, since almost 100% of the respondents reported always conducting weed monitoring. High use of herbicides during fallow period is the result of the wide adoption of NT crop production (91% in 2019/20) (Asociación Argentina de Productores en Siembra Directa, 2020Asociación Argentina de Productores en Siembra Directa – Aapresid. [Evolution of direct sowing: 2019/20 campaign]. Rosario: Asociación Argentina de Productores en Siembra Directa; 2020[access Nov 14, 2022]. Spanish. Available from: https://www.aapresid.org.ar/archivos/evolucion-siembra-directa2019-2020.pdf..
https://www.aapresid.org.ar/archivos/evo...
; Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales, 2022Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
). Producers commonly use chemical fallow by applying residual and postemergence herbicides to control problematic facultative winter weeds such as Conyza sp. (Walker et al., 2012Walker S, Boucher L, Cook T, Davidson B, McLean A, Widderick M. Weed age affects chemical control of Conyza bonariensis in fallows. Crop Prot. 2012;38:15-20. Available from: https://doi.org/10.1016/j.cropro.2012.03.008
https://doi.org/10.1016/j.cropro.2012.03...
), Lolium sp. and S. oleraceus. They also use herbicides to manage early spring-emerging species like D. sanguinalis, E. indica and Echinochloa crus-galli (L.) P. Beauv. before planting summer crops. The percentage of producers and consultants adopting crop rotation with the response "always" increased from 45% in the previous survey to nearly 67% when considering those responding with "almost always," and when including those adopting crop rotation "often," it reached nearly 98%. This was also observed by the reduction of 2.2 million hectares planted with soybean between the last survey and the present with an increase in corn, wheat and sunflower areas by 2.4, 1.6 and 0.1 million hectares respectively (Bolsa Cereales, 2024Bolsa Cereales. [Reports and data]. Buenos Aires: Bolsa de Cereales; 2024[access May 1, 2024]. Spanish. Available from: https://www.bolsadecereales.com/estimaciones-informes
https://www.bolsadecereales.com/estimaci...
). The intensification of crop rotations could significantly reduce herbicide use without negatively affecting the functional structure or species richness of the weed community (de la Fuente et al., 2021bFuente EB, Oreja FH, Lenardis AE, Fuentes MT, Agosti B, Barrio A et al. Intensification of crop rotation affecting weed communities and the use of herbicides in the rolling Pampa. Heliyon. 2021b;7(1):1-13. Available from: https://doi.org/10.1016/j.heliyon.2021.e06089
https://doi.org/10.1016/j.heliyon.2021.e...
). Among Argentinean producers, the benefits of crop rotation are widely accepted, not only for weed management but also for disease control and improving soil properties. However, the final decision often hinges on short-term crop profitability considerations (Dury et al., 2012Dury J, Schaller N, Garcia F, Reynaud A, Bergez JE. Models to support cropping plan and crop rotation decisions: a review. Agron Sust Develop. 2012;32(2):567-80. Available from: https://doi.org/10.1007/s13593-011-0037-x
https://doi.org/10.1007/s13593-011-0037-...
).

Figure 4
Use of weed management practices expressed as a percentage of the following categories: always, often, sometimes, and never. MOA, mode of action

The rotation of herbicide modes of action (MOA) and measures to prevent weed seed production increased to 20% compared to Scursoni et al. (2019)Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
. Repeated applications of herbicides with the same MOA accelerate herbicide-resistance evolution, such as, several applications of glyphosate in glyphosate-HR soybean monoculture (or glyphosate-HR cotton) in some regions of the United States (Beckie, 2006Beckie HJ. Herbicide-resistant weeds: management tactics and practices. Weed Technol. 2006;20(3):793-814. Available from: https://doi.org/10.1614/WT-05-084R1.1
https://doi.org/10.1614/WT-05-084R1.1...
). Highly simplified systems with minimal crop and herbicide MOA rotation favor weed communities with fewer difficult-to-control species, for instance, the overuse of glyphosate or ACCase- and ALS-inhibiting herbicides (Storkey, Neve, 2018Storkey J, Neve P. What good is weed diversity? Weed Res 2018;58(4):239-43. Available from: https://doi.org/10.1111/wre.12310
https://doi.org/10.1111/wre.12310...
; Oreja et al., 2021Oreja FH, Inman MD, Jordan DL, Leon RG. Population growth rates of weed species in response to herbicide programme intensity and their impact on weed community. Weed Res. 2021;61(6):509-18. Available from: https://doi.org/10.1111/wre.12509
https://doi.org/10.1111/wre.12509...
). The increasing number of herbicide-resistant weed populations (Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
) has prompted producers to adopt herbicide MOA rotation. Among the BMP to delay resistance (Norsworthy et al., 2012Norsworthy JK, Ward SM, Shaw DR, Llewellyn RS, Nichols RL, Webster TM et al. Reducing the risks of herbicide resistance: best management practices and recommendations. Weed Sci. 2012;60(SP1):31-62. Available from: https://doi.org/10.1614/WS-D-11-00155.1
https://doi.org/10.1614/WS-D-11-00155.1...
), MOA rotation is the most widely accepted and adopted by producers. The reduction of 2.2 M ha of soybean and the increase in area of other summer crops, such as corn and sunflower, means that producers are rotating MOA from one crop to the other in fields. Another widely adopted practice is preventing weed seed production, with 36% of respondents reporting its adoption. Late-season weed control may incur additional short-term costs for producers, but it offers long-term benefits by reducing the seedbank size (Bagavathiannan, Norsworthy, 2012Bagavathiannan MV, Norsworthy JK. Late-season seed production in arable weed communities: management im-plications. Weed Sci. 2012;60(3):325-34. Available from: https://doi.org/10.1614/WS-D-11-00222.1
https://doi.org/10.1614/WS-D-11-00222.1...
).

The adoption of practices aimed at enhancing crop competitive ability, such as the adoption of competitive cultivars and adjusting row spacing, increased by 15% to 18%. Approximately 8% of respondents reported making modifications to planting dates and seeding density. The cleaning of tillage and harvesting equipment and the application of the recommended herbicide rate increased by 12% for the "always" response. A significant 78% responded that they always applied the recommended herbicide rate, while the remaining 10% responded with "often". As stated by Scursoni et al. (2019)Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
, these practices do not typically imply high costs for producers and yet are still not fully integrated into weed management programs. The adoption of cover crops reached a total of 75% of respondents when considering the "often" and "always" responses together, representing a 35% increase compared to the previous survey. (Scursoni et al., 2019Scursoni JA, Vera ACD, Oreja FH, Kruk BC, Fuente EB. Weed management practices in Argentina crops. Weed Technol. 2019;33(3):459-63. Available from: https://doi.org/10.1017/wet.2019.26
https://doi.org/10.1017/wet.2019.26...
). The proportion of producers practicing cover crops and consultants recommending this practice "always" (15%) is similar to the 19% reported in the survey by ReTAA (2022)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
. The benefits of cover crops in reducing weed pressure are well-known among producers (Laloy, Bielders, 2010Laloy E, Bielders CL. Effect of intercropping period management on runoff and erosion in a corn cropping system. J Environ Qual. 2010;39(3):1001-8. Available from: https://doi.org/10.2134/jeq2009.0239
https://doi.org/10.2134/jeq2009.0239...
; Bergtold et al., 2017Bergtold JS, Ramsey S, Maddy L, Williams JR. A review of economic considerations for cover crops as a conservation practice. Renew Agric Food Syst. 2017;34(1):62-76. Available from: https://doi.org/10.1017/S1742170517000278
https://doi.org/10.1017/S174217051700027...
). The adoption of cover crops is influenced by various factors, including demographic characteristics, establishment practices, adoption of related management practices, environmental attitudes, and climate (Lee, McCann, 2019Lee S, McCann L. Adoption of cover crops by US soybean producers. J Agric Appl Econ. 2019;51(4):527-44. Available from: https://doi.org/10.1017/aae.2019.20
https://doi.org/10.1017/aae.2019.20...
). Practices with mid to long-term benefits could be a significant adoption barrier (Bergtold et al., 2012Bergtold JS, Duffy PA, Hite D, Raper RL. Demographic and management factors affecting the perceived benefit of winter cover crops in the southeast. J Agric Appl Econ. 2012;44(1):99-116. Available from: https://doi.org/10.1017/S1074070800000195
https://doi.org/10.1017/S107407080000019...
). This concern is particularly relevant in the context of farming on rented land, which constitutes a substantial portion of Argentina's agriculture. Additionally, worries regarding water and nutrient consumption, as well as sowing costs, may limit the widespread adoption of cover crops. Depending on the climate and seasonal precipitation, delaying the termination of cover crops may deplete soil moisture, negatively impacting cash crops (Balkcom et al., 2015Balkcom KS, Duzy LM, Kornecki TS, Price AJ. Timing of cover crop termination: management considerations for the southeast. Crop Forage Turf Manag. 2015;1(1):1-7. Available from: https://doi.org/10.2134/cftm2015.0161
https://doi.org/10.2134/cftm2015.0161...
).

Finally, the use of targeted herbicide application and the adoption of predictive models were the only practices some respondents declared "never" considering in their weed management plans (Figure 4). Despite the increasing availability and well-documented benefits of predictive models, their adoption by producers is still low globally (Evans et al., 2017Evans KJ, Terhorst A, Kang BH. From data to decisions: helping crop producers build their actionable knowledge. Crit Rev Plant Sci. 2017;36(2):71-88. Available from: https://doi.org/10.1080/07352689.2017.1336047
https://doi.org/10.1080/07352689.2017.13...
). According to Wilkerson et al. (2002)Wilkerson GG, Wiles LJ, Bennett AC. Weed management decision models: pitfalls, perceptions, and possibilities of the economic threshold approach. Weed Sci. 2002;50(4):411-24. Available from: https://doi.org/10.1614/0043-1745(2002)050[0411:WMDMPP]2.0.CO;2
https://doi.org/10.1614/0043-1745(2002)0...
, producers have cited several reasons for avoiding the use of models, including the perception that some are too simplistic, based on a single species, neglect spatial distribution of weeds, or are overly complex and require excessive information. The limited adoption of targeted herbicide application is primarily due to its high cost and the absence of explicit short-term monetary benefits (Tidemann et al., 2017Tidemann BD, Hall LM, Harker KN, Beckie HJ. Factors affecting weed seed devitalization with the Harrington seed destructor. Weed Sci. 2017;65(5):650-8. Available from: https://doi.org/10.1017/wsc.2017.23
https://doi.org/10.1017/wsc.2017.23...
).

3.4 Herbicide use

Glyphosate was the most commonly applied herbicide, with more than 90% of responses indicating very frequent use across different crops. These findings align with those reported by ReTAA (2023)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
, where glyphosate ranked as the most used herbicide in terms of the number of applications per season among the most important crops in the country. Since the adoption of GR crops in the mid-90s, the global use of glyphosate has increased significantly (Clapp, 2021Clapp J. Explaining growing glyphosate use: the political economy of herbicide-dependent agriculture. Global Environ Change. 2021;67. Available from: https://doi.org/10.1016/j.gloenvcha.2021.102239
https://doi.org/10.1016/j.gloenvcha.2021...
), and a similar trend has been observed in Argentina (Penna, Lema, 2003Penna JA, Lema D. Adoption of herbicide tolerant soybeans in Argentina: an economic analysis. In: Kalaitzandonakes N, editor. Economic and environmental impacts of agrotechnology. New York: Kluwer-Plenum; 2003. p. 203-220.). The majority of soybean and corn genotypes cultivated in Argentina are GR (Consejo Argentino para la Información y el Desarrollo de la Biotecnología, 2022Consejo Argentino para la Información y el Desarrollo de la Biotecnología – Argenbio. [Transgenic crops]. Buenos Aires: Consejo Argentino para la Información y el Desarrollo de la Biotecnología; 2022[access Nov 5, 2022]. Spanish. Available from: https://argenbio.org/cultivos-transgenicos
https://argenbio.org/cultivos-transgenic...
), especially under NT systems (Asociación Argentina de Productores en Siembra Directa, 2020Asociación Argentina de Productores en Siembra Directa – Aapresid. [Evolution of direct sowing: 2019/20 campaign]. Rosario: Asociación Argentina de Productores en Siembra Directa; 2020[access Nov 14, 2022]. Spanish. Available from: https://www.aapresid.org.ar/archivos/evolucion-siembra-directa2019-2020.pdf..
https://www.aapresid.org.ar/archivos/evo...
). Among the non-selective herbicides used in fallow before summer crops, paraquat has been more commonly used than glufosinate. This is despite the availability of summer crop genotypes tolerant to glufosinate in the market (Consejo Argentino para la Información y el Desarrollo de la Biotecnología, 2022Consejo Argentino para la Información y el Desarrollo de la Biotecnología – Argenbio. [Transgenic crops]. Buenos Aires: Consejo Argentino para la Información y el Desarrollo de la Biotecnología; 2022[access Nov 5, 2022]. Spanish. Available from: https://argenbio.org/cultivos-transgenicos
https://argenbio.org/cultivos-transgenic...
). This pattern is consistent with the findings of a report by Bolsa Cereales (2023Bolsa Cereales. [Datasets]. Bolsa Cereales. 2023[access May 15, 2023]. Spanish. Available from: https://www.bolsadecereales.com/datasets
https://www.bolsadecereales.com/datasets...
), where paraquat was also more frequently used than glufosinate in terms of the number of applications and doses, except in the case of corn. Notably, glufosinate-tolerant corn genotypes have been available in Argentina since 1998, which is earlier than the introduction of glufosinate-tolerant genotypes for other crops such as soybean (the first glufosinate-tolerant genotype was released in 2011) and wheat (the first glufosinate-tolerant genotype was released in 2020) (Consejo Argentino para la Información y el Desarrollo de la Biotecnología, 2022Consejo Argentino para la Información y el Desarrollo de la Biotecnología – Argenbio. [Transgenic crops]. Buenos Aires: Consejo Argentino para la Información y el Desarrollo de la Biotecnología; 2022[access Nov 5, 2022]. Spanish. Available from: https://argenbio.org/cultivos-transgenicos
https://argenbio.org/cultivos-transgenic...
). In North Carolina, glufosinate has been rarely applied initially, primarily due to the availability of other effective herbicides (Jones et al., 2022Jones EA, Cahoon CW, Leon RG, Everman WJ. Surveying stakeholder's perception of glufosinate and use in North Carolina. Weed Technol. 2022; 36(3):443-450. Available from: https://doi.org/10.1017/wet.2022.31
https://doi.org/10.1017/wet.2022.31...
), which may also be the case for other crops apart from corn. Furthermore, a significant proportion of Argentinean producers rent fields in mid-spring when Conyza sp. plants are at an advanced phenological stage, making control challenging. To address this issue, especially in soybean crop, producers employ the double-knockdown technique, which involves the application of glyphosate plus 2,4-D followed by paraquat (Walsh, Poles, 2007Walsh MJ, Powles SB. Management strategies for herbicide-resistant weed populations in Australian dryland crop production systems. Weed Technol. 2007;21(2):332-8. Available from: https://doi.org/10.1614/WT-06-086.1
https://doi.org/10.1614/WT-06-086.1...
). Another reason for the increasing use of non-selective herbicides like paraquat is the proliferation of GR weeds (Heap, 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
; Oreja et al., 2024Oreja FH, Moreno N, Gundel PE, Vercellino RB, Pandolfo CE, Presotto A et al. Herbicide-resistant weeds from dryland agriculture in Argentina. Weed Res. 2024;64(2):89-106. Available from: https://doi.org/10.1111/wre.12613
https://doi.org/10.1111/wre.12613...
). Nearly 90% of those surveyed reported using the double-knockdown technique, with responses indicating that 12% always used it, 23% often used it, and 54% used it sometimes (Figure 5). Finally, it should be noted that the use of glufosinate was usually more expensive than the use of paraquat, which may explain why growers prefer paraquat instead of glufosinate.

Figure 5
Use of herbicides in summer crops and fallow expressed as a percentage of the following categories: always, often, sometimes, and never

Summer crops. In terms of auxinic herbicides (Group 4), the most commonly used were 2,4-D (80% always/often) and dicamba (57% always/often) as a burndown strategy previous to soybean and corn planting, and as post emergent herbicide in corn during the period emergence to V4 stage. Despite being one of the earliest herbicides used in extensive agriculture, 2,4-D continues to be widely used as a chlorophenoxy product (Peterson et al. 2016Peterson MA, McMaster SA, Riechers DE, Skelton J, Stahlman PW. 2,4-D past, present, and future: a review. Weed Technol. 2016;30(2):303-45. Available from: https://doi.org/10.1614/WT-D-15-00131.1
https://doi.org/10.1614/WT-D-15-00131.1...
). This is due to its high efficacy against broadleaf weeds, a relatively low incidence of herbicide-resistant cases (Heap 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
), and its cost-effectiveness compared to other herbicides. These findings align with herbicide usage patterns reported by ReTAA (2022)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
, where 2,4-D was the most commonly used auxin herbicide across all crops, and dicamba was consistently the second most applied auxin herbicide. When considering herbicides with long-term activity in the soil, atrazine, a photosystem II-inhibiting herbicide, was more frequently used (65.7% always/often) than sulfonylureas and imidazolinones, which are ALS-inhibiting herbicides (35%). Atrazine serves as the primary residual herbicide employed in corn cultivation for the control of broadleaf weeds. Its usage has also increased during the fallow period preceding soybean planting to manage Conyza sp. during the winter and early spring (Wu et al., 2008Wu H, Walker S, Robinson G. Chemical control of flaxleaf fleabane (Conyza bonariensis (L.) Cronquist) in winter fallows. Plant Prot Quat. 2008;23(4):162-5.). According to ReTAA (2022)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
, atrazine was the most commonly adopted residual herbicide in corn during the 2019/2020 and 2020/2021 seasons. Conversely, in wheat crops, the most commonly used residual herbicides belonged to the sulfonylurea family (Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales, 2022Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
).

Regarding preemergence herbicides, sulfentrazone (a protoporphyrinogen oxidase [PPO]-inhibiting herbicide), flumioxazin (PPO-inhibiting herbicide) and S-metolachlor (very long-chain fatty acid synthesis inhibiting-herbicide) (Group 15) were the most commonly used (60% often/always). These findings align with those of ReTAA (2022)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
, which reported sulfentrazone and flumioxazin as the most widely used preemergence herbicides in soybean and S-metolachlor in corn. Among the graminicides, clethodim was the more frequently used option (70% often/always) compared to haloxyfop-methyl (44%). This preference for clethodim can be attributed to the increasing number of cases of S. halepense resistance to haloxyfop-methyl in recent years (Heap 2023Heap I. The International Herbicide-Resistant Weed Database. Weedscience. 2023[access Jan 29, 2023]. http://www.weedscience.orgwww.weedscience.org
http://www.weedscience.orgwww.weedscienc...
), a trend also noted by ReTAA (2022)Relevamiento de Tecnología Agrícola Aplicada de la Bolsa de Cereales – ReTAA. [Environmental practices in Argentine agricultural production]. Informes Mensual 53. Feb 23, 2022[access Sept 26, 2022]. Spanish. Available from: https://www.bolsadecereales.com/download/comunicados_contenidos/documento1/1753
https://www.bolsadecereales.com/download...
. In the context of controlling broadleaf weeds in soybeans, particularly Amaranthus sp., fomesafen (a PPO-inhibiting herbicide) was the preferred choice (31% often/always), surpassing lactofen (a PPO-inhibiting herbicide) at 7% and benazolin (an auxin herbicide) at 5%.

Winter crops. Among the auxinic herbicides, both in winter cereals and summer crops, 2,4-D was more frequently used, with 83.4% reporting its use always or often, compared to dicamba at 69% and picloram at 18%. It's worth noting that the use of metsulfuron-methyl (an ALS-inhibiting herbicide) was similar to dicamba but is specifically included in wheat management for residual control. Mixtures of iodosulfuron-methyl-Na + mesosulfuron-methyl (both ALS-inhibiting herbicides) and pinoxaden (an ACCase-inhibiting herbicides) were applied to control grasses in wheat. During the fallow period preceding the wheat crop, clethodim and haloxyfop-methyl were applied to control GR Lolium spp. At pre-planting, the application rates of flurochloridone, flumioxazin, and pyroxasulfone were similar, ranging from 8 to 10% for "always" and "often" responses (Figure 6).

Figure 6
Use of herbicides in wheat and previous fallow expressed as a percentage of the following categories: always, often, sometimes, and never

4. Conclusions

The survey provided a valuable information about most troublesome weeds, weed management practices adopted and herbicide use in the main Argentinian crop producing regions. The most troublesome weeds according to the survey are C. bonariensis, D. sanguinalis, E. indica, A. hybridus and S. halepense and all of these species have herbicide-resistant biotypes in Argentina. Survey results showed that weed management is still very dependent on herbicides and MOA rotation, but producers are beginning to adopt IWM to a certain extent. Despite producers unknowingly adopting IWM, it is necessary to highlight that non-chemical practices do not imply high costs for producers to accelerate IWM adoption. Future management recommendations should focus on IWM strategies to avoid the evolution of herbicide resistance and weed community shifts.

Describing the different types of weed management, and quantifying their frequency, allow the diagnosis and the identification of possible causes and consequences of the adoption of agricultural practices, opening the door to the development of public policies that promote good agricultural practices, conserve the environment and guarantee security in access to markets. This diagnosis is a useful tool to plan political strategies at regional scale promoting the combination of different tactics and to implement proactive integrated management decisions at field scale reducing and preventing weed problems in Argentina, and the approach may be useful to analyze weed problems in extensive crops worldwide.

Acknowledgements

This research was financially supported by the University of Buenos Aires (UBACyT 20020190100285BA). We thank all those who kindly responded to the survey and to those that spread the survey, the Argentine Asociation of Weed Science (ASACIM) and Agroconsultas Online. Thanks to Dr. E. A. Jones for his comments and criticisms of the original manuscript.

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Edited by

Approved by:
Editor in Chief: Carlos Eduardo Schaedler
Associate Editor: Jingxu Zhang

Publication Dates

  • Publication in this collection
    09 Sept 2024
  • Date of issue
    2024

History

  • Received
    06 Oct 2023
  • Accepted
    08 July 2024
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