Abstract
We report the identification of a 48kDa antigen targeted by antibodies which inhibit Plasmodium falciparum in vitro growth by cooperation with blood monocytes in an ADCI assay correlated to the naturally acquired protection. This protein is located on the surface of the merozoite stage of P. falciparum, and is detectable in all isolates tested. Epidemiological studies demonstrated that peptides derived from the amino acid sequence of MSP-3 contain potent B and T-cell epitopes recognized by a majority of individuals living in endemic areas. Moreover human antibodies either purified on the recombinant protein, or on the synthetic peptide MSP-3b, as well as antibodies raised in mice, were all found to promote parasite killing mediated by monocytes.
malaria; Plasmodium falciparum; merozoite; MSP-3
A novel merozoite surface antigen of Plasmodium falciparum (MSP-3) identified by cellular-antibody cooperative mechanism antigenicity and biological activity of antibodies
Claude Oeuvray1
Hasnaa Bouharoun-Tayoun1
Heléne Grass-Masse2
Jean Paul Iepers3
Laurence Ralamboranto3
André Tartar4
Pierre Druilhe1
Institut Pasteur, Laboratoire de Parasitologie Médicale, Paris, France
Institut Pasteur de Lille, Laboratoire de Chimie des Bio-Molécules, Lile, France
URA, CNRS, Lile, France
Institut Pasteur de Madagascar, Unité de Recherche sur le Paludisme, Antananarivo, Madagascar
We report the identification of a 48kDa antigen targeted by antibodies which inhibit Plasmodium falciparum in vitro growth by cooperation with blood monocytes in an ADCI assay correlated to the naturally acquired protection. This protein is located on the surface of the merozoite stage of P. falciparum, and is detectable in all isolates tested. Epidemiological studies demonstrated that peptides derived from the amino acid sequence of MSP-3 contain potent B and T-cell epitopes recognized by a majority of individuals living in endemic areas. Moreover human antibodies either purified on the recombinant protein, or on the synthetic peptide MSP-3b, as well as antibodies raised in mice, were all found to promote parasite killing mediated by monocytes.
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Publication Dates
-
Publication in this collection
15 June 2009 -
Date of issue
1994