Migration of Schistosoma mansoni sambon (Trematoda, Schistosomatidae) from skin to lungs in immunized NZ rabbits (Lagomorpha, Leporidae) by autoradiographic analysis

Pinto, R. Magalhães; Noronha, Dely; Almeida, Marília S. S; Katz, Naftale; Tendler, Miriam

doi:10.1590/S0101-81751994000400006

Abstract

Skin to lung migration of Schistosoma mansoni Sambon, 1907 - (75 Se) - selenomethionine-labeled cercariae was tracked by tissue autoradiography on days 1, 4, 6, 8 after challenge, in rabbits immunized with a S. mansoni derived saline extract. Either in vaccinated animals or in those of the control unprimed group, the peak of skin schistosomula occurs 24hs after infection. Comparison between peaks of lungs migrating larvae showed that, in control animals, the increase of worm burden in this site, is detected on the 6th day post-infection, differing from immunized rabbits, in which this peak occurs on day 4, when skin and lungs counts are still equivalent, decreasing gradually, showing a different pattern of the S. mansoni migration and suggesting that main parasite attrition occurs during the late skin and early lung phases in the immunized group.

Schistosoma mansoni; migration; autoradiography; immunization; rabbits

Migration of Schistosoma mansoni sambon (Trematoda, Schistosomatidae) from skin to lungs in immunized NZ rabbits (Lagomorpha, Leporidae) by autoradiographic analysis

R. Magalhães Pinto^I; Dely Noronha^I; Marília S. S. Almeida^I; Naftale Katz^II; Miriam Tendler^I

^IDepartamento de Helmintologia, Instituto Oswaldo Cruz (FIOCRUZ), Caixa Postal 926, 21045-900 Rio de Janeiro, Rio de Janeiro, Brasil

^IICentro de Pesquisas René Rachou (FIOCRUZ), 39220-000 Belo Horizonte, Minas Gerais, Brasil

ABSTRACT

Skin to lung migration of Schistosoma mansoni Sambon, 1907 - (⁷⁵ Se) - selenomethionine-labeled cercariae was tracked by tissue autoradiography on days 1, 4, 6, 8 after challenge, in rabbits immunized with a S. mansoni derived saline extract. Either in vaccinated animals or in those of the control unprimed group, the peak of skin schistosomula occurs 24hs after infection. Comparison between peaks of lungs migrating larvae showed that, in control animals, the increase of worm burden in this site, is detected on the 6th day post-infection, differing from immunized rabbits, in which this peak occurs on day 4, when skin and lungs counts are still equivalent, decreasing gradually, showing a different pattern of the S. mansoni migration and suggesting that main parasite attrition occurs during the late skin and early lung phases in the immunized group.

Key words:Schistosoma mansoni, migration, autoradiography, immunization, rabbits

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ACKNOWLEDGEMENTS. To Dr. Nicolau Maués da Serra Freire, Universidade Federal Rural do Rio de Janeiro (UFRRJ), for the graphic transposition of the results.

BIBLIOGRAPHIC REFERENCES

Recebido em 01.II.1994; aceito em 08.VIII.1994.

References

Dean, D.A. & B.L. Mangold. 1984. Autoradiographic analysis of resistance to reinfection with Schistosoma mansoni in mice. Evidence that the liver is major site of worm elimination. Am. J. Trop. Med. Hyg. 33: 97-103.
Dean, D.A.; B.L. Mangold; J.R. Georgi & R.H. Jacobson. 1984. Comparison of Schistosoma mansoni_migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis. Am. J. Trop. Med. Hyg. 33: 89-96.
Chandiwana, S.K. 1988. Use of ⁷⁵Se tracer and autoradiographic techniques in the study of schistosomiasis. Parasitology 97: 480-502.
Georgi, J.R. 1982. Schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay autoradiography. Parasitology 84: 263-281.
Georgi, J.R.; D.A. Dean & S.K. Chandiwana. 1982. Quantification of Schistosoma mansoni in mouse lungs by radioassay and autoradiography of ⁷⁵Se-labeled schistosomula. J. Parasitol. 68: 1092-1095.
Georgi, J.R.; D.A. Dean & B.L. Mangold. 1983. Schistosoma mansoni: temporal distribution of radioselenium-labelled schistosomula in lungs of mice during the first two weeks of infection. Parasitology 86: 31-36.
Karnija, H. & D.J. Mclaren. 1987. Schistosoma mansoni: migration potential and radiation attenuated parasites in naive guinea pigs. Exp. Parasitol 63: 98-107.
Knopf, P.M.; D. Cioli; B.L. Mangold & D.A. Dean. 1986. Migration of Schistosoma mansoni in normal and passively immunized laboratory rats. Am. J. Trop. Med. Hyg. 35: 1173-1184.
Mangold, B.L. & D.A. Dean. 1984. The migration and survival of gamma-irradiated Schistosoma mansoni larvae and the duration of host-parasite contact in relation to the indution of resistance in mice. Parasitology 88: 249-266.
Miller, P. & R.A. Wilson. 1978. Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology 77: 281-302.
Pearce, E.J. & D.J. Mclaren. 1983. Reappraisal of the guinea pig as an experimental host for studies of schistosomiasis mansoni. Parasitology 87: 455-464.
Pinto, R.M.; D. Noronha; M.S.S. Almeida; M. Tendler; N. Katz & S.S. Palma, 1987, Schistosoma mansoni: migration patterns in normal and immunized Swiss Webster mice by means of autoradiographic analysis. Mem. Inst. Oswaldo Cruz 82: 231-232.
Pinto, R.M.; D. Noronha; M.S.S. Almeida; M. Tendler & N. Katz. 1990. Autoradiographic analysis of Schistosoma mansoni migration in the NZ rabbit. Mem. Inst. Oswaldo Cruz 85: 91-94.
Smithers, S.R. & R.J. Terry. 1965. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55: 695-700.
Tendler, M.; A.O. Lima; R.M. Pinto; M.Q. Cruz; H.M. Brascher & N. Katz. 1982. Immunogenic and protective activity of an extract of Schistosoma mansoni. Mem. Inst. Oswaldo Cruz 77: 275-283.
Tendler, M.; R.M. Pinto; A.O. Lima; G. Gebara & N. Katz. 1986. Schistosoma mansoni: vaccination with adult worm antigens. Int. J. Parasitol. 16: 347-352.
Tendler, ML; M.S.S. Almeida; R.M. Pinto; D. Noronha & N. Katz. 1991. Schistosoma mansoni - New Zealand rabbit model: resistance induced by infection followed by active immunization with protective antigens. J. Parasitol. 77: 138-141.
Wilson, R.A.; P. Coulson & B. Dixon. 1986. Migration of the schistosomula of Schistosoma mansoni in mice vaccinated with radiation-attenuated cercariae and normal mice: an attempt to identify the timing and site of parasite death. Parasitology 92: 101-116.

Publication Dates

Publication in this collection
31 July 2009
Date of issue
1994

History

Accepted
08 Aug 1994
Received
01 Feb 1994

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Dean, D.A. & B.L. Mangold. 1984. Autoradiographic analysis of resistance to reinfection with Schistosoma mansoni in mice. Evidence that the liver is major site of worm elimination. Am. J. Trop. Med. Hyg. 33: 97-103.

[2] Dean, D.A.; B.L. Mangold; J.R. Georgi & R.H. Jacobson. 1984. Comparison of Schistosoma mansoni_migration patterns in normal and irradiated cercaria-immunized mice by means of autoradiographic analysis. Am. J. Trop. Med. Hyg. 33: 89-96.

[3] Chandiwana, S.K. 1988. Use of ⁷⁵Se tracer and autoradiographic techniques in the study of schistosomiasis. Parasitology 97: 480-502.

[4] Georgi, J.R. 1982. Schistosoma mansoni: quantification of skin penetration and early migration by differential external radioassay autoradiography. Parasitology 84: 263-281.

[5] Georgi, J.R.; D.A. Dean & S.K. Chandiwana. 1982. Quantification of Schistosoma mansoni in mouse lungs by radioassay and autoradiography of ⁷⁵Se-labeled schistosomula. J. Parasitol. 68: 1092-1095.

[6] Georgi, J.R.; D.A. Dean & B.L. Mangold. 1983. Schistosoma mansoni: temporal distribution of radioselenium-labelled schistosomula in lungs of mice during the first two weeks of infection. Parasitology 86: 31-36.

[7] Karnija, H. & D.J. Mclaren. 1987. Schistosoma mansoni: migration potential and radiation attenuated parasites in naive guinea pigs. Exp. Parasitol 63: 98-107.

[8] Knopf, P.M.; D. Cioli; B.L. Mangold & D.A. Dean. 1986. Migration of Schistosoma mansoni in normal and passively immunized laboratory rats. Am. J. Trop. Med. Hyg. 35: 1173-1184.

[9] Mangold, B.L. & D.A. Dean. 1984. The migration and survival of gamma-irradiated Schistosoma mansoni larvae and the duration of host-parasite contact in relation to the indution of resistance in mice. Parasitology 88: 249-266.

[10] Miller, P. & R.A. Wilson. 1978. Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology 77: 281-302.

[11] Pearce, E.J. & D.J. Mclaren. 1983. Reappraisal of the guinea pig as an experimental host for studies of schistosomiasis mansoni. Parasitology 87: 455-464.

[12] Pinto, R.M.; D. Noronha; M.S.S. Almeida; M. Tendler; N. Katz & S.S. Palma, 1987, Schistosoma mansoni: migration patterns in normal and immunized Swiss Webster mice by means of autoradiographic analysis. Mem. Inst. Oswaldo Cruz 82: 231-232.

[13] Pinto, R.M.; D. Noronha; M.S.S. Almeida; M. Tendler & N. Katz. 1990. Autoradiographic analysis of Schistosoma mansoni migration in the NZ rabbit. Mem. Inst. Oswaldo Cruz 85: 91-94.

[14] Smithers, S.R. & R.J. Terry. 1965. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55: 695-700.

[15] Tendler, M.; A.O. Lima; R.M. Pinto; M.Q. Cruz; H.M. Brascher & N. Katz. 1982. Immunogenic and protective activity of an extract of Schistosoma mansoni. Mem. Inst. Oswaldo Cruz 77: 275-283.

[16] Tendler, M.; R.M. Pinto; A.O. Lima; G. Gebara & N. Katz. 1986. Schistosoma mansoni: vaccination with adult worm antigens. Int. J. Parasitol. 16: 347-352.

[17] Tendler, ML; M.S.S. Almeida; R.M. Pinto; D. Noronha & N. Katz. 1991. Schistosoma mansoni - New Zealand rabbit model: resistance induced by infection followed by active immunization with protective antigens. J. Parasitol. 77: 138-141.

[18] Wilson, R.A.; P. Coulson & B. Dixon. 1986. Migration of the schistosomula of Schistosoma mansoni in mice vaccinated with radiation-attenuated cercariae and normal mice: an attempt to identify the timing and site of parasite death. Parasitology 92: 101-116.